This video is a full and clear explanation about the principle and the applications of agarose gel electrophoresis.
Жүктеу.....
Пікірлер: 104
@romelsoyza41606 жыл бұрын
A complete and very easy to understand explanation (like the videos for Flow Cytometry and FACS) on the principles behind the technique.
@AyazSamo5 жыл бұрын
She is great a great teacher! Respects from Sindh!
@joan-b-empire576 Жыл бұрын
Love from Cameroon 🇨🇲 thank you
@VarshiiiPrem5 жыл бұрын
A great teacher..... Thanks a lot... Tomorrow is my exam... Very useful
@mohammedholba24986 жыл бұрын
One of the best youtube chanel l ever seen. :)
@walrus42484 жыл бұрын
Will see if you already did capillary electrophoresis, this was such a fine explanation thanks.
@ciao_abhi6 жыл бұрын
This helped me so much for my mcat test
@mohanndri645 жыл бұрын
Such a good and simply way of explanation thank you
@user-ff9vu8ic7b Жыл бұрын
بجد احسن حد شرح الموضوع دة بالتوفيق❤
@gilyasungi45725 жыл бұрын
Well explained! Good explanations linked with real life applications.
@ladushky13 жыл бұрын
Thank you! we have never add the colour right into the gel tho.. but we do add it to the samples we are running on the gel.
@ridzwanfauzi9754 жыл бұрын
great video!!really helped me in my test..thank you so much
@mohammedal-hammadi50855 жыл бұрын
Wow, this video is so clear and useful, besides you are awesome, thank you so much
@RohitPant043 жыл бұрын
Concise & informative! Good job. 🙏
@muhammadjaber22726 жыл бұрын
Your voice like best for presenting and I have get many from your channel
@walrus42484 жыл бұрын
Fantastic, I learned a lot and I didn't know anything about the subject.
@tiaxi67795 жыл бұрын
very informative video, explained in detail for the reason of each step.
@Gbemi784 жыл бұрын
your explanation is second to none.
@dstan162244 жыл бұрын
Thank you very much ma'am.It really helped a lot.
@rahmanebrahimzadegan71982 жыл бұрын
You teach excellent. Thank you.
@Animelover79482 жыл бұрын
Very very well explained...very helpful....people out there can try watching this vid
@poetrylover55613 жыл бұрын
You've nicely explained the topic. Many thanks for sharing ♥️
@sukanyahembrom62137 жыл бұрын
very well explained.... thanx alot for making such video
@Amar456113 жыл бұрын
Thank you Ma'am.. It's a great video! ❤
@srigayathri59802 жыл бұрын
Thank you so muck mam.Because of u i can able to understand the concept now mam.
@worldaviation4k4 жыл бұрын
so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
@bahahos74264 жыл бұрын
Thank u very much i love all your videos
@maggiejameel67255 жыл бұрын
Very well explained, you are an awesome teacher. I SUBSCRIBED
@krisantinimarkam3396 Жыл бұрын
Very well explained and easy to understand. Thank you
@girlschannel115 жыл бұрын
Really good explanation !!!!
@statusboys40362 жыл бұрын
God give me an opportunity to spend 15 mins of my life time in a precious way...
@wonderakpese1901 Жыл бұрын
Concept well explained. Thank you
@qamarhennawi91372 жыл бұрын
You are the best, no one like you
@sanskrutiraj805111 ай бұрын
This video is very helpful for me. Thank you u explained in easy way 🙂
@tanimaferdous506 жыл бұрын
Very very good explanation
@bharathkumart60975 жыл бұрын
Thank you very much for this video ma'am
@yennguyen-tp7ce6 жыл бұрын
everything is just so clear, thanks
@ikhinerawlings124
Жыл бұрын
Well explained. Thanks. Can talk on chromatography in details
@pritichristian26732 жыл бұрын
Thanks. Very clear explanation
@setarehsohail54222 жыл бұрын
It was a very nice lecture!
@abdulmunafisalisuumar70165 ай бұрын
thank you so much maam, for your wonderful explanation of Principle of Agarose gel electrophoresis.
@trona26125 жыл бұрын
Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
@shailendrayadav57994 жыл бұрын
You are great teacher mam
@halafr58615 жыл бұрын
You are the best thank you from my heart 💜
@stephaniehurtado40962 жыл бұрын
Late to the upload but I’ve watch almost all your video and they all been so helpful:) ofc ill like and subscribe!
@insanhabib44974 жыл бұрын
Thanks a lot, ma'am.
@Min-gh1jr6 жыл бұрын
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
@Katherine-mf9wzАй бұрын
Nice job!
@jyothinandakumar619011 ай бұрын
Very well explained
@iliyamohd81543 жыл бұрын
Very good presentation
@krishnanandh79993 жыл бұрын
Supr class. Thank you🤩🤩
@Az-xr5yx5 жыл бұрын
Thanx thats was helpfull
@soheilaazam7994 жыл бұрын
You are the best molec teacher, who I have ever seen!!! Well done 👍😊 and thanks for the Videos. What is your name by the way???
@yamaatomayi43156 жыл бұрын
mem i like your explanation, i love to hear u r voice again and again u r a great talent .. and i really love..
@briang1310
5 жыл бұрын
why do indian people always says mem or ma'am
@SandeepKumar-dd3ie
4 жыл бұрын
@@briang1310 coz it is taught and its logical as well to respect the efforts
@gabecalderon2043
2 жыл бұрын
@@SandeepKumar-dd3ie learn respect then
@tinyikonkuna14792 жыл бұрын
😊😊😊😊Love it here plz make more videos on biology concepts especially 4 varsity students
@dikshashreedevi45685 жыл бұрын
please make some more videos .good explanation
@fasilutubechannel66972 жыл бұрын
thanks i hope another video release in this related video
@robinkhan64192 жыл бұрын
Well presentation
@jiaflair2 жыл бұрын
THANKYOU 💞
@saraabdulaziz37642 жыл бұрын
Very informative🥰
@sahewa1006 жыл бұрын
Very well explained.... thanx alot for making such video. This helped a lot in my thesis research. Please continue to teach us :))
@biomedicalandbiologicalsci4989
6 жыл бұрын
Thank you for your support :)
@thnxm82 жыл бұрын
Thanks a lot
@joyeke63402 жыл бұрын
Thank you
@felixjohnpaulbarqueros66755 жыл бұрын
Hi how can I determine the effectiveness if i will alternative coloring dye for the loading buffer
@geeswags61722 жыл бұрын
Informative
@stevemorton30784 жыл бұрын
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
@Milahh.7 жыл бұрын
Thx, very useful well done
@biomedicalandbiologicalsci4989
7 жыл бұрын
Thank you for your comment ...
@samanthabautista16555 жыл бұрын
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
@ranaaamiraamir34375 жыл бұрын
Brilliant
@jaswantnegi48794 жыл бұрын
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
@MaryGraceBayot Жыл бұрын
Thanks
@nextagro3 жыл бұрын
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
@claudiaaurie81616 жыл бұрын
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
@biomedicalandbiologicalsci4989
6 жыл бұрын
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
@sidratulmuntaha1687
5 жыл бұрын
@@biomedicalandbiologicalsci4989 Can we search it out from the literature which endonuclease to be used?
@kuldeepdinkar49186 жыл бұрын
What is the use of nylon fiber?
@lincolnkarim13 жыл бұрын
This is not my field at all so please excuse my ignorance; Since DNA is a negative molecule (3:48), does that mean it is an ion?
@drgaikwadsir72705 жыл бұрын
Make some videos related with r dDNA technology
@jthomas00072 жыл бұрын
Hi ma'am, could you please talk about PCR and it's various types?
@urmilahussainpiya67355 жыл бұрын
please make a vedio why Taq DNA polymerase is thermostable
@emanwanli77403 жыл бұрын
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
@drgaikwadsir72705 жыл бұрын
Nice mam
@devyaniitware24285 жыл бұрын
What are amplicons?
@stevanstankovic80214 жыл бұрын
Great video, thanks! I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
@ladushky1
3 жыл бұрын
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
@redeemerbadagbor16732 ай бұрын
you do all :salut
@youcare64157 жыл бұрын
Very informative. I guess it is SYBR Green not cyber green ;)
@anirbanhait5998
2 жыл бұрын
Ma'am have pronounced rightly.
@SLNmin
Жыл бұрын
It is SYBR, pronounced as cyber but correct spelling is SYBR.
@Zeenatullah3473 ай бұрын
❤❤❤
@wycliffenyandika9017 Жыл бұрын
like it
@Art-cq1zy2 жыл бұрын
Im confused. Why does the solution not evaporate in the microwave?
@sukantamandal81064 жыл бұрын
Replication
@jsvclubdeciencia62833 жыл бұрын
In silico: kzread.info/dash/bejne/dJ-ItLuGmtzHpKQ.html
@blackseastorm613 жыл бұрын
I don't think anything in nature and universe is junk or useless
@ladushky13 жыл бұрын
Sorry, but there is no such thing as DNA junk sequence anymore.
@nagendrag75623 жыл бұрын
explained very nicely
@abeerm97864 жыл бұрын
Thank you
@samanthabautista16555 жыл бұрын
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
Пікірлер: 104
A complete and very easy to understand explanation (like the videos for Flow Cytometry and FACS) on the principles behind the technique.
She is great a great teacher! Respects from Sindh!
Love from Cameroon 🇨🇲 thank you
A great teacher..... Thanks a lot... Tomorrow is my exam... Very useful
One of the best youtube chanel l ever seen. :)
Will see if you already did capillary electrophoresis, this was such a fine explanation thanks.
This helped me so much for my mcat test
Such a good and simply way of explanation thank you
بجد احسن حد شرح الموضوع دة بالتوفيق❤
Well explained! Good explanations linked with real life applications.
Thank you! we have never add the colour right into the gel tho.. but we do add it to the samples we are running on the gel.
great video!!really helped me in my test..thank you so much
Wow, this video is so clear and useful, besides you are awesome, thank you so much
Concise & informative! Good job. 🙏
Your voice like best for presenting and I have get many from your channel
Fantastic, I learned a lot and I didn't know anything about the subject.
very informative video, explained in detail for the reason of each step.
your explanation is second to none.
Thank you very much ma'am.It really helped a lot.
You teach excellent. Thank you.
Very very well explained...very helpful....people out there can try watching this vid
You've nicely explained the topic. Many thanks for sharing ♥️
very well explained.... thanx alot for making such video
Thank you Ma'am.. It's a great video! ❤
Thank you so muck mam.Because of u i can able to understand the concept now mam.
so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
Thank u very much i love all your videos
Very well explained, you are an awesome teacher. I SUBSCRIBED
Very well explained and easy to understand. Thank you
Really good explanation !!!!
God give me an opportunity to spend 15 mins of my life time in a precious way...
Concept well explained. Thank you
You are the best, no one like you
This video is very helpful for me. Thank you u explained in easy way 🙂
Very very good explanation
Thank you very much for this video ma'am
everything is just so clear, thanks
@ikhinerawlings124
Жыл бұрын
Well explained. Thanks. Can talk on chromatography in details
Thanks. Very clear explanation
It was a very nice lecture!
thank you so much maam, for your wonderful explanation of Principle of Agarose gel electrophoresis.
Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
You are great teacher mam
You are the best thank you from my heart 💜
Late to the upload but I’ve watch almost all your video and they all been so helpful:) ofc ill like and subscribe!
Thanks a lot, ma'am.
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
Nice job!
Very well explained
Very good presentation
Supr class. Thank you🤩🤩
Thanx thats was helpfull
You are the best molec teacher, who I have ever seen!!! Well done 👍😊 and thanks for the Videos. What is your name by the way???
mem i like your explanation, i love to hear u r voice again and again u r a great talent .. and i really love..
@briang1310
5 жыл бұрын
why do indian people always says mem or ma'am
@SandeepKumar-dd3ie
4 жыл бұрын
@@briang1310 coz it is taught and its logical as well to respect the efforts
@gabecalderon2043
2 жыл бұрын
@@SandeepKumar-dd3ie learn respect then
😊😊😊😊Love it here plz make more videos on biology concepts especially 4 varsity students
please make some more videos .good explanation
thanks i hope another video release in this related video
Well presentation
THANKYOU 💞
Very informative🥰
Very well explained.... thanx alot for making such video. This helped a lot in my thesis research. Please continue to teach us :))
@biomedicalandbiologicalsci4989
6 жыл бұрын
Thank you for your support :)
Thanks a lot
Thank you
Hi how can I determine the effectiveness if i will alternative coloring dye for the loading buffer
Informative
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
Thx, very useful well done
@biomedicalandbiologicalsci4989
7 жыл бұрын
Thank you for your comment ...
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
Brilliant
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
Thanks
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
@biomedicalandbiologicalsci4989
6 жыл бұрын
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
@sidratulmuntaha1687
5 жыл бұрын
@@biomedicalandbiologicalsci4989 Can we search it out from the literature which endonuclease to be used?
What is the use of nylon fiber?
This is not my field at all so please excuse my ignorance; Since DNA is a negative molecule (3:48), does that mean it is an ion?
Make some videos related with r dDNA technology
Hi ma'am, could you please talk about PCR and it's various types?
please make a vedio why Taq DNA polymerase is thermostable
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
Nice mam
What are amplicons?
Great video, thanks! I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
@ladushky1
3 жыл бұрын
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
you do all :salut
Very informative. I guess it is SYBR Green not cyber green ;)
@anirbanhait5998
2 жыл бұрын
Ma'am have pronounced rightly.
@SLNmin
Жыл бұрын
It is SYBR, pronounced as cyber but correct spelling is SYBR.
❤❤❤
like it
Im confused. Why does the solution not evaporate in the microwave?
Replication
In silico: kzread.info/dash/bejne/dJ-ItLuGmtzHpKQ.html
I don't think anything in nature and universe is junk or useless
Sorry, but there is no such thing as DNA junk sequence anymore.
explained very nicely
Thank you
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please