The principle of SDS PAGE-a full and clear explanation of the technique and how does it work

Ғылым және технология

This video is to understand everything about SDS-PAGE, its principle, the technique, the discontinuous gel system, and more.
2D gel Electrophoresis video:
• The principle of 2D Ge...
Western Blotting video:
• Blotting Techniques/ T...

Пікірлер: 294

  • @Kevinsungeun
    @Kevinsungeun7 жыл бұрын

    Why can't my professor just memorise this video and rap it during the lab. Very useful!

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    7 жыл бұрын

    Haha thank you ... maybe you can recommend it to your colleagues ;)

  • @MrPridizzle

    @MrPridizzle

    7 жыл бұрын

    throw that ish on Rap Chat yo, that would be FIRE!

  • @julietten5614

    @julietten5614

    6 жыл бұрын

    your professor probs done thousands of 'gels' while doing his first research work. I m assuming he's just not interested any more:-))

  • @tartanhandbag

    @tartanhandbag

    5 жыл бұрын

    this is why professors should do research and teachers should teach. not always the case; some professors are excellent, lively, dynamic and patient teachers also ...but most just want to get back to overseeing their projects, publishing papers and finishing grant applications before the dreaded deadlines.

  • @jimmyolum4344

    @jimmyolum4344

    3 жыл бұрын

    Hehe

  • @gabrieldynkin5078
    @gabrieldynkin50786 жыл бұрын

    I just have to say it was the best explantion i've ever heard about anything! Thank you, it answered all of my questions....:)

  • @4MoonGlade4
    @4MoonGlade46 жыл бұрын

    Hello! This video really saved me! Detailed and exhaustive. I was studying this technique for an exam and didn't understand it really...now it's all clear. Thanks! 😊

  • @brindabandas3341
    @brindabandas33412 жыл бұрын

    This is the first technique video which I have completely understood without any practical experience. Thank you so much for making such a great video.

  • @UQMD
    @UQMD4 жыл бұрын

    Thanks for the fantastic video! We just watched your video in class and it definitely helped us understand electrophoresis. I especially loved the pace of your video :)

  • @saniyapamnani8494
    @saniyapamnani84942 жыл бұрын

    This is the best video I found on the internet about SDS PAGE. Thank you so much for all the details.

  • @ismailgbadamosi8321
    @ismailgbadamosi83214 жыл бұрын

    This is the best video I have seen on the working principle of SDS PAGE. Thank you

  • @murielmirus5641
    @murielmirus56414 жыл бұрын

    thank you so much for the hard work you put into this video! it is very much appreciated :) your video has helped me a lot in the lab today!

  • @medinlab141
    @medinlab1413 жыл бұрын

    Thank you so much for the explanation, especially for the stacking gel's part! I found it very clear.

  • @tedwills-topic9524
    @tedwills-topic95242 жыл бұрын

    This helped me a lot before even doing my practicals. Thank you

  • @RevesAbsurdes1
    @RevesAbsurdes16 жыл бұрын

    Perfect ! I spend time looking for those informtion on internet and you give them (and a lot more) very clearly, good job !

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    6 жыл бұрын

    Hello, thank you for your comment ... happy you got what you need from the video :)

  • @RevesAbsurdes1

    @RevesAbsurdes1

    6 жыл бұрын

    Biomedical and Biological Sciences yes i actually have an exam about all those technics soon and you saved my life haha !

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    6 жыл бұрын

    Wow, this is good ... I have a playlist on my channel containing videos about all the electrophoresis techniques, have a look at it, it will help you for your exam: kzread.info/dash/bejne/gaKTvNGtlL3LqKw.html

  • @justcat6042
    @justcat60426 жыл бұрын

    This is the most helpful video i have ever watched about sds page. Now i understand much more about this! Thank you so much

  • @ranahaddad5446
    @ranahaddad54462 жыл бұрын

    I had watched many videos to understand this topic, but this video is definitely the best! Thank you so much!

  • @daniellegoodwin3517
    @daniellegoodwin35173 жыл бұрын

    Thanks so much for a very clear explanation! I tried to understand the technique by reading a description online but it was so confusing, thanks for clearing it up for me!

  • @KJKP
    @KJKP6 жыл бұрын

    More detailed than other videos on SDS-PAGE.

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    6 жыл бұрын

    yup, stay tuned for more videos :)

  • @maharmibhattacharya4327
    @maharmibhattacharya43275 жыл бұрын

    thank you so much, the explanation was very descriptive and reasonably good.

  • @swagpunjabiada6521
    @swagpunjabiada65216 жыл бұрын

    Mam please keep uploading such videos so that we can have clear concept about each technique ur way of teaching is great😄

  • @emilievu4846
    @emilievu48465 жыл бұрын

    Very well explained! Do you have any sources for this? I would like to refer to it in my masters.

  • @julymgreenday1993
    @julymgreenday19936 жыл бұрын

    I finally undertood Electrophoresis!!!! THANK YOU! Greetings from Argentina ♥

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    6 жыл бұрын

    Hei, Glade you liked the video ... stay tuned :)

  • @sumantahalder1027
    @sumantahalder10277 жыл бұрын

    I am too pleased to watch your videos, these are very clear concept growing video. Thank you very much keep it up.

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    7 жыл бұрын

    Thank you for your comment ... I will ... Stay tuned :)

  • @harveenkaur1713
    @harveenkaur17136 жыл бұрын

    The most helpful video ever! Thank you so much!

  • @rogsang9764
    @rogsang97642 жыл бұрын

    thanks for the explanation, the best thing is that you dedicate time to explain the principle of the gel, its the best explanation i´ve seen so far :D

  • @junghyunlee1714
    @junghyunlee17147 жыл бұрын

    Umm... I have one question. At the stacking gel, speed of the ions is Cl(-)>protein>glycine(n/-). In my opinion, Cl is faster than glycine these distance gonna be far each other. I wanna know details what is happened in stacking gel and these speed.

  • @richardjoy8522
    @richardjoy85223 жыл бұрын

    Really really really good explanation...I was searching for this type of detailed explanation in whole of KZread.....

  • @giorgiasartori6334
    @giorgiasartori63343 жыл бұрын

    you are so amazing, keep going like this!! this is THE ONLY VIDEO i found explained well

  • @bobmvideos
    @bobmvideos3 жыл бұрын

    OMG thanks, my wife has been doing these for like 25 years, now I know what the hell she has been talking about. Now to watch your video on westerns...

  • @francestorreysirdevan4774
    @francestorreysirdevan47745 жыл бұрын

    Superb video!! Insanely helpful! Thank you so much!!

  • @sylaslau302
    @sylaslau3026 жыл бұрын

    Thank you!! It's comprehensive and concise at the same time. I wonder what would be the technical difference (composition and steps) between this and Urea-PAGE for ssDNA or RNA.

  • @shreyasen5614
    @shreyasen56145 жыл бұрын

    Love the video! Very informative. Thank you so much

  • @ZAIUAMED
    @ZAIUAMED7 жыл бұрын

    Very helpful keep it up.

  • @MuhammadRizwan-dt5us
    @MuhammadRizwan-dt5us6 жыл бұрын

    i find it very useful thankyou so much for make that kind of videos

  • @mariakln9654
    @mariakln96545 жыл бұрын

    Congrats! Very helpful video, I understood everything I needed for my lab. Thank you! (the explanation is very good and well constructed)

  • @roulala8993
    @roulala89934 жыл бұрын

    Thank you very much for the video. You're a good teacher. 😊

  • @soniyahalan9051
    @soniyahalan90516 жыл бұрын

    Thank you so much video is very helpful to understand better SDS page

  • @allysadati9710

    @allysadati9710

    4 жыл бұрын

    True

  • @sundasshareef8691
    @sundasshareef86916 жыл бұрын

    Perfect .👍 simply wht i was actually searching for🙌

  • @allaboutfootball5729
    @allaboutfootball57296 жыл бұрын

    Thank u very much u r wonderful keep making these videos I learnt a lot and many things become clear

  • @yeny7194
    @yeny71943 жыл бұрын

    Thank you this helps a lot! the article i'm reading talks about 3 different gels running gel, gradient gel and stacking gel, In this video we are shown separating gel, which one of the two (running or gradient gels) would the separating gel be equivalent to?

  • @Laladear-sz4hz
    @Laladear-sz4hz5 жыл бұрын

    thanku for clearing my doubts about this techniques

  • @ashiqahamed7740
    @ashiqahamed77404 жыл бұрын

    Very well put together. loved this video Thank you :)

  • @joannchu14
    @joannchu144 ай бұрын

    Beautiful explanation! Love this ❤

  • @pavanhebbar5843
    @pavanhebbar58432 жыл бұрын

    For SDS, I just watch this... Thank you for this indepth information mam🙏🙏

  • @claudiaaurie8161
    @claudiaaurie81616 жыл бұрын

    very good explanation. Thank u!

  • @simiralucialamberty5971
    @simiralucialamberty59716 жыл бұрын

    Your videos are excellent!

  • @punithagr3339
    @punithagr33396 жыл бұрын

    Clear and to the point. Thank you

  • @avishekdas180
    @avishekdas1804 жыл бұрын

    Nicely done yet again.

  • @bharathreddy9337
    @bharathreddy93376 жыл бұрын

    I wish I will have a teacher like you in the future. terrific and detailed explanation

  • @orfeasorfeas9346
    @orfeasorfeas93465 жыл бұрын

    I finally understood it! Thanks!

  • @alfankoshable1
    @alfankoshable15 жыл бұрын

    thank you very informative ! keep going from Saudi arabia Salam

  • @abidakhadim1935
    @abidakhadim19356 жыл бұрын

    Thank you mam .for giving a good visual memory of SDS.page .

  • @elmaspower2356
    @elmaspower23563 жыл бұрын

    Awesomely explained.. Thanks very much mam. Lots of love and respect from Bangladesh

  • @batuhantekmen6607
    @batuhantekmen66073 жыл бұрын

    Why and how proteins have been aligned in the stacking gel? Thank you.

  • @adityasinha1166
    @adityasinha11663 жыл бұрын

    Thankyou so much for the explanation. I loved it 😊😊. Good work.

  • @dancingbiologist2688
    @dancingbiologist26883 жыл бұрын

    This was very helpful! Thank you!

  • @leticiaalvescavalcante9593
    @leticiaalvescavalcante95937 жыл бұрын

    OH MY GOD!!!! Thank you SO much!!! I'm a student from Brazil and I was looking for something that would make me understand the electrophoresis tecnique and I was not findind anything so simple and easy like this video! I'm so happy right now... I was almost giving up haha I was reading, and reading, and reading and not understanding at all, but now is everything so clear in my mind because of you...Omg, THANK YOUUUUU!!!!!!

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    7 жыл бұрын

    WOOW .. thank you for the nice comment ... stay tuned, you might find everything you are searching for in this channel :)

  • @colin-kun3611
    @colin-kun36113 жыл бұрын

    Solid video! Thanks a million times! Subbed!

  • @priyadharshini-jx6jz
    @priyadharshini-jx6jz6 жыл бұрын

    So clear ,good job 👍

  • @Ash-be1nv
    @Ash-be1nv2 жыл бұрын

    extremely helpful video for beginners

  • @genomics3754
    @genomics37544 жыл бұрын

    thank you mam....for such a well explained lecture.

  • @preciousndzimandze4348
    @preciousndzimandze43482 жыл бұрын

    So helpful, thanks so much!

  • @geralthasan
    @geralthasan2 жыл бұрын

    you have my thanks madam for saving me from mid exam disaster. Thank you so much

  • @elenatantardini4932
    @elenatantardini49324 жыл бұрын

    Best WB video ever!

  • @danielhernandezbanuelos5581
    @danielhernandezbanuelos55816 жыл бұрын

    thank you, very well explained.

  • @ruchinambiar8005
    @ruchinambiar80053 жыл бұрын

    This video was very useful. Thank you ma'am

  • @kaushikilohiya2137
    @kaushikilohiya21374 жыл бұрын

    This is a really great explanation of d topic

  • @TheQaseeh
    @TheQaseeh6 жыл бұрын

    thank you!! clear and detail explanation....

  • @trish88881
    @trish888813 жыл бұрын

    This is so useful. Life Saver!

  • @vavakhannan3335
    @vavakhannan33354 жыл бұрын

    Very well explained........thank you so much mam

  • @physicstutor9127
    @physicstutor91274 жыл бұрын

    Very helpful. Thank you!

  • @kanayoikeh363
    @kanayoikeh3635 жыл бұрын

    Thank you for the really great explanation. I still don't quite understand why the stacking gel is needed in the first place. Can someone please help?

  • @aydamohamed7983
    @aydamohamed79833 жыл бұрын

    Thnx sooooo much. Very clear and important

  • @pannalalroat5394
    @pannalalroat53943 жыл бұрын

    Really such a amazing explanation 😘🤩

  • @muhammadashraf-iy1gd
    @muhammadashraf-iy1gd10 ай бұрын

    Outstanding explanation ❤

  • @StalwartsCo
    @StalwartsCo7 жыл бұрын

    indeed best video for beginners.

  • @krishnanandh7999
    @krishnanandh79993 жыл бұрын

    Very informative. Thank you😍

  • @MayankSingh-uj6hi
    @MayankSingh-uj6hi6 жыл бұрын

    Very good explanation. Thank u sooo much mam

  • @poetrylover5561
    @poetrylover55613 жыл бұрын

    Very nice explanation of a difficult concept of SDS PAGE 👍👍👍👍

  • @evelinjoseph5008
    @evelinjoseph50084 жыл бұрын

    Just amazing....y der are no more videos posted? It will b great if u post about centrifuge

  • @shdrone94
    @shdrone943 жыл бұрын

    very good explained !

  • @uzoechisamuel
    @uzoechisamuel2 жыл бұрын

    And somebody has been messing with my brain all this while. Anytime I see you in person, I will give you a bottle of win.

  • @darkgamingyt1659
    @darkgamingyt16593 жыл бұрын

    Superb explaination

  • @pkjaihind3472
    @pkjaihind34723 жыл бұрын

    Excellent Explanation 👍👏👏👏 Keep it up 👍

  • @biotechnologymahasarakhamu4757
    @biotechnologymahasarakhamu47577 жыл бұрын

    Awesome teaching. Thank you so much.

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    7 жыл бұрын

    welcome ... Thank you for the comment :)

  • @susmita93
    @susmita936 жыл бұрын

    Very good explanation ...really impressed..please make biochemistry molecular biology cell biology related videos to help us..thank you

  • @user-bc1hv2cc9p
    @user-bc1hv2cc9p4 жыл бұрын

    thank you so much it's very helpful

  • @smart9924
    @smart99245 жыл бұрын

    Mam your videos are very good. It is very easy to understand this. Mam can you please make a video on immunoelectrophoresis. Thank you so much 😊

  • @sadeghsadeghzadeh8170
    @sadeghsadeghzadeh81706 жыл бұрын

    It was very useful. Thanks alot

  • @Umbrella_Corporation1
    @Umbrella_Corporation16 жыл бұрын

    how much do voltage apply for SDS PAGE unit for the protein gel seperation ???

  • @ikz8439
    @ikz84392 жыл бұрын

    you're helpful thanks!

  • @ruhiyyihdyrdahl-young3846
    @ruhiyyihdyrdahl-young38467 жыл бұрын

    Thanks what a great explanation!

  • @biomedicalandbiologicalsci4989

    @biomedicalandbiologicalsci4989

    7 жыл бұрын

    You are welcome :D

  • @realmetv2970
    @realmetv29702 жыл бұрын

    Such a perfect video

  • @ArielStar
    @ArielStar5 жыл бұрын

    Amazingly explained, thank you!!

  • @debjitdey216
    @debjitdey2163 жыл бұрын

    Finally I understood 🤗🤗🤗 thank uuuuuuu

  • @shivaprasad5446
    @shivaprasad54463 жыл бұрын

    well explained, thanks for the video :)

  • @ARD100
    @ARD1005 жыл бұрын

    Principally what should be the contents of cell lysis buffer for preparing protein samples for SDS PAGE

  • @firasbn1582
    @firasbn15822 жыл бұрын

    Thank you for the Video

  • @deegallarupananda6927
    @deegallarupananda6927 Жыл бұрын

    Thank you very much!

  • @sunnychatterjee5487
    @sunnychatterjee54873 жыл бұрын

    Explanation is fantastic but it will very well if you explain how stacking and separating gel is prepared...

  • @nabilamursyidah5416
    @nabilamursyidah54163 жыл бұрын

    thank you for the video. can you make a video of how to read the protein electrophoresis result?

  • @M7MD99.
    @M7MD99. Жыл бұрын

    كثر الله خيرك

  • @user-eq8mc2hi3y
    @user-eq8mc2hi3y3 жыл бұрын

    Oh my god so useful video... you save my grade

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