Spectrophotometry | Beer-Lambert Law.
This video is about Spectrophotometry, and discusses in details the Spectrophotometer and Beer-Lambert's law.
In this video I discuss the relationship between the absorbance and the concentration:
- A=epsilon x l x c
- how to determine experimentally the molar absorptivity
- find the concentration of an unknown solution.
You can also watch an experiment using the Beer-Lambert law:
• AP Chemistry Investiga...
Students studying Chemistry at different levels could highly benefit from this video.
Пікірлер: 95
I hope you find this video helpful. Please note that the answer at 5:25 is 5.0 x 10^(-3) and not 5.0 x 10^(-2) as it is shown in the video. The preparation for such videos takes lots of time and sometimes small mistakes like this slip easily. Enjoy!
@freeradical9123
5 жыл бұрын
Nice video easy to understand From India 🤓👌
@eldhose5691
4 жыл бұрын
Double beam
@snehamathivanan7679
4 жыл бұрын
Thanks a lot...very informative video...
@shanequalawrence4782
4 жыл бұрын
i am not too understanding how you get the power
@maimoonamumtaz6376
3 жыл бұрын
Thank you sir... Your student from Pakistan 👍👍👍💓💓
Woah, everything was so clear. This video is those kinds of videos where you can pause on a certain time and have all the information you need neatly placed with explanations and everything. Thanks man :D
Really clear and easy to follow explanation. Good job.
Really good video! helpful and informative and easy to understand, really good job! thank you so much!:)
fantastic!! thank you. very clear and perfect pacing.
Thank you so much I was facing problems in this topic but now all is clear
You explained it really fast and clear. Thank you for this video it will help me in a labolatory!
Amazing explanation. Thanks a lot!
You really saved my life. God bless your heart . Thank you so much for sharing your brilliant knowledge 😊 🙏
THANK U VERY MUCH THE DIAGRAM WITH DESCRIBTION IS VERY USEFUL
I am watching this in 2018, you are really great Sir, Good explanation, you deserve million subscribers but it's sad nowadays people wants to watch only Crap, youth is destroying their Time & Career.
Nice informative video. Thank you very much for sharing with us.
Very helpful video. Easy to understand. Thanks.
Awesome video sir! Thank you!
Best video on Beer lamberts law... Thanks And keep making such videos
راااائع جد..نريد المزيد من الفيديو
Thank you very much. It is really appreciated.🙏
lovely explanation thank you!
man you do a good job thx keep up good work ;)
Wow! I really appreciate it Sir
thanks, that's was great it was very helpful
The explanation was helpful, thank you
very good my man,u explain nicely
thank u so muchh.. such a good video :)
Wow. Great explanation
thanks, you are really great sir
Video was very helpful sir thx a lot
Gud lecture sir gathered more information
Excellent lecture
Thank you! It s so easier!
Great explanation! 👍🙏🙏
Good one bro
I have a test about this next week and truth to be told, I did not understood a thing about it, this video helped me to clear it all up so thank you for taking the time to make this. It was really helpful!
@AliHayek
6 жыл бұрын
+LuciferHasPinkSparklyWings you are more than welcome.
Thank you very much Sir
tx for uploading very helpful
thank you very much mate!
dude please make more chemistry videos.... every second of the video had a purpose.
Outstanding
Thanx bro it really helpful
Nice and very useful
thnq so much sir
helpful video thaaaaaank you so much
Gud lecture sir and I have few queries regarding biotechnology
well presented Shukran
Love this video
nice work sir
Nice explanation Ali Hayek
JazakALLAH
Thank u so much sir
Very good video
thank you sir
Time point 5:33, I think there is a mistake: 0.5 and not 5.0
bruh you deserve an emmy for this vid
Thank you
Hello Dr. Hayek, Thank you very much for this video. The explanation is clear, well-organized, and getting on point, which helps me a lot. I have a few questions. Is there any chemical relation between the concentration of the samples and the incident light emitting or absorbing? I am getting unstable absorbance values with measurements at low concentrations but not at high concentrations. This finding is also beyond the understanding of Absorbance = logarithms of transmission though my values are at 0.1% of T. or could you please enlighten me on the keywords I could find for the articles? Thanks a lot.
@AliHayek
Жыл бұрын
Hi there, Light-matter interaction is a known phenomenon. Sometimes it does not affect the structure of the matter, but some other times light can be aggressive and provides enough energy to break weak bonds. If the absorbance is not stable or keeps changing, it could be that the light is transforming the matter into something that interacts less/more with light. It is a broad topic. I hope this will allow you to start your search. Best of luck.
Tq,sir
Thanks very much for this helpful video. But I wanna ask about the references, please?
Nice
You are doing excellent job. A little humor will make it perfect. Can you tell me what software you are using for making these videos.
@AliHayek
6 жыл бұрын
+BioMania thank you. I use screenflow
Is I0 actually the intensity of light as read by detector when a sample of pure solvent (water) is placed in the cuvette?
@AliHayek
6 жыл бұрын
Nick F yes it is indeed. And this is called the blank and it is used for the background noise correction.
nice
Please upload beer law for mixture
Super
Hello the video is wonderful. I can not understand one thing which is when we look the transmittance vs wavelenght graph, why do we observe this shape or when we look the absorption vs wavelenght graph, why do we observe this shape? Thank you so much
@AliHayek
4 жыл бұрын
The best way to think about it is that for the absorption spectrum, the higher the intensity of the peak, the more the light is being absorbed by the solution. Now if you think about it in terms of transmittance. The more the light is absorbed, the less of it will transmit. So transmittance measures how much light is going through the solution. So the lower the peak, the lower the percentage of light transmitted. In a simpler way, the transmittance spectrum is the opposite of the absorption spectrum. I hope it is clear now.
great video but the answer is 5.0 x 10-3 not 5.0 x 10-2. the video really help me thanks btw.
@debasishmajhi687
6 жыл бұрын
WATCHDATFREE u know right that calculators of different types gives different value if u don't know then Google it
@kelw5787
6 жыл бұрын
Yeah I calculated this as well and when I heard it was 5.0 x 10-2 I was confused about what I did wrong xD
@rahullovesthepayne8690
6 жыл бұрын
i thought i was the only one lol
Good day sir, is the molar coefficient is a constant given per chemical substance or it's something that we can compute too? I am wondering how am I be able to get the Molar Coefficient of unknown Species and Unknown Concentration, that is present in the water for example lake. This is for spectrophotometer experiment, I can only get the Absorbance and Transmittance but not the Concentration and Molar Coefficient
@AliHayek
4 жыл бұрын
In your case you will not be able to determine the concentration or molar extinction coefficient. You will need one of them known to determine the other.
Suuuupppppeerrrrr
At 2:54 % transmittance is denoted as %T and also in last line as A. Didn't get it. Please explain
@AliHayek
6 жыл бұрын
If you read carefully the last line says that the absorbance (A) can be given in terms of (%T) and the expression is given. I hope this helps.
I am watching from Uzbekistan. How can I contact you?
@AliHayek
Жыл бұрын
Go to www.alihayek.com. 👍
Sir how find (£)coficiant value Please reply
@AliHayek
3 жыл бұрын
This is explained in details by the end of the video. This is the only way to find epsilon. Good luck.
@premalathagattu907
3 жыл бұрын
@@AliHayek thank you sir it is very useful video
Answer is 5 x 10^-3
more simple calculation of perceent transmittance or OD vaalues in to Percentage will be more useful for basic chemistry students, videos explainigncalculations msy not be available
watch this uv visible spectroscopy is explained very briefly... @
بس لو بالعربي كان فهمت
:)
tx for uploading very helpful