Spectrophotometry | Beer-Lambert Law.

This video is about Spectrophotometry, and discusses in details the Spectrophotometer and Beer-Lambert's law.
In this video I discuss the relationship between the absorbance and the concentration:
- A=epsilon x l x c
- how to determine experimentally the molar absorptivity
- find the concentration of an unknown solution.
You can also watch an experiment using the Beer-Lambert law:
• AP Chemistry Investiga...
Students studying Chemistry at different levels could highly benefit from this video.

Пікірлер: 95

  • @AliHayek
    @AliHayek6 жыл бұрын

    I hope you find this video helpful. Please note that the answer at 5:25 is 5.0 x 10^(-3) and not 5.0 x 10^(-2) as it is shown in the video. The preparation for such videos takes lots of time and sometimes small mistakes like this slip easily. Enjoy!

  • @freeradical9123

    @freeradical9123

    5 жыл бұрын

    Nice video easy to understand From India 🤓👌

  • @eldhose5691

    @eldhose5691

    4 жыл бұрын

    Double beam

  • @snehamathivanan7679

    @snehamathivanan7679

    4 жыл бұрын

    Thanks a lot...very informative video...

  • @shanequalawrence4782

    @shanequalawrence4782

    4 жыл бұрын

    i am not too understanding how you get the power

  • @maimoonamumtaz6376

    @maimoonamumtaz6376

    3 жыл бұрын

    Thank you sir... Your student from Pakistan 👍👍👍💓💓

  • @jackyllerena5336
    @jackyllerena53366 жыл бұрын

    Woah, everything was so clear. This video is those kinds of videos where you can pause on a certain time and have all the information you need neatly placed with explanations and everything. Thanks man :D

  • @1babyflix
    @1babyflix6 жыл бұрын

    Really clear and easy to follow explanation. Good job.

  • @Isaac-ru5jb
    @Isaac-ru5jb6 жыл бұрын

    Really good video! helpful and informative and easy to understand, really good job! thank you so much!:)

  • @EJ-qs6qh
    @EJ-qs6qh5 жыл бұрын

    fantastic!! thank you. very clear and perfect pacing.

  • @karanmakwana5129
    @karanmakwana51295 жыл бұрын

    Thank you so much I was facing problems in this topic but now all is clear

  • @marzenasemaj7204
    @marzenasemaj7204 Жыл бұрын

    You explained it really fast and clear. Thank you for this video it will help me in a labolatory!

  • @aayataltamimi9325
    @aayataltamimi93256 жыл бұрын

    Amazing explanation. Thanks a lot!

  • @michaelseraphim1056
    @michaelseraphim10562 жыл бұрын

    You really saved my life. God bless your heart . Thank you so much for sharing your brilliant knowledge 😊 🙏

  • @MrAbdalla1996
    @MrAbdalla19966 жыл бұрын

    THANK U VERY MUCH THE DIAGRAM WITH DESCRIBTION IS VERY USEFUL

  • @shivammkhare
    @shivammkhare5 жыл бұрын

    I am watching this in 2018, you are really great Sir, Good explanation, you deserve million subscribers but it's sad nowadays people wants to watch only Crap, youth is destroying their Time & Career.

  • @md.ibrahimkhalil6011
    @md.ibrahimkhalil60116 жыл бұрын

    Nice informative video. Thank you very much for sharing with us.

  • @SquatBenchDeadliftz
    @SquatBenchDeadliftz5 жыл бұрын

    Very helpful video. Easy to understand. Thanks.

  • @KulbirAhluwalia
    @KulbirAhluwalia6 жыл бұрын

    Awesome video sir! Thank you!

  • @dr.pratikk8930
    @dr.pratikk89304 жыл бұрын

    Best video on Beer lamberts law... Thanks And keep making such videos

  • @sajamas5766
    @sajamas57667 жыл бұрын

    راااائع جد..نريد المزيد من الفيديو

  • @khinmyatthu9289
    @khinmyatthu9289 Жыл бұрын

    Thank you very much. It is really appreciated.🙏

  • @rabiadegia4361
    @rabiadegia43616 жыл бұрын

    lovely explanation thank you!

  • @ramamukumbi5566
    @ramamukumbi55667 жыл бұрын

    man you do a good job thx keep up good work ;)

  • @tiibrahim5714
    @tiibrahim57146 жыл бұрын

    Wow! I really appreciate it Sir

  • @manuelperezruizdiaz9418
    @manuelperezruizdiaz94186 жыл бұрын

    thanks, that's was great it was very helpful

  • @loyalty6495
    @loyalty64953 жыл бұрын

    The explanation was helpful, thank you

  • @dancemabu6341
    @dancemabu63417 жыл бұрын

    very good my man,u explain nicely

  • @tarkeshkandregula6559
    @tarkeshkandregula65597 жыл бұрын

    thank u so muchh.. such a good video :)

  • @shrishantroy2494
    @shrishantroy24943 жыл бұрын

    Wow. Great explanation

  • @edusmart1126
    @edusmart11265 жыл бұрын

    thanks, you are really great sir

  • @debasishmajhi687
    @debasishmajhi6876 жыл бұрын

    Video was very helpful sir thx a lot

  • @jeevaniroyal8407
    @jeevaniroyal84075 жыл бұрын

    Gud lecture sir gathered more information

  • @jamespandiannaraj5744
    @jamespandiannaraj57445 ай бұрын

    Excellent lecture

  • @TheDina132
    @TheDina1323 жыл бұрын

    Thank you! It s so easier!

  • @dannyjean-noeluppiah8270
    @dannyjean-noeluppiah82703 жыл бұрын

    Great explanation! 👍🙏🙏

  • @shubhambhosale4723
    @shubhambhosale47236 жыл бұрын

    Good one bro

  • @TemeraireBjartskula
    @TemeraireBjartskula6 жыл бұрын

    I have a test about this next week and truth to be told, I did not understood a thing about it, this video helped me to clear it all up so thank you for taking the time to make this. It was really helpful!

  • @AliHayek

    @AliHayek

    6 жыл бұрын

    +LuciferHasPinkSparklyWings you are more than welcome.

  • @sylviaemejuru1956
    @sylviaemejuru19564 жыл бұрын

    Thank you very much Sir

  • @binteabdul6107
    @binteabdul61075 жыл бұрын

    tx for uploading very helpful

  • @domlehane2819
    @domlehane28197 жыл бұрын

    thank you very much mate!

  • @adanalvarez6830
    @adanalvarez68304 жыл бұрын

    dude please make more chemistry videos.... every second of the video had a purpose.

  • @oumarshah1335
    @oumarshah13352 жыл бұрын

    Outstanding

  • @piyushsen7502
    @piyushsen75025 жыл бұрын

    Thanx bro it really helpful

  • @mohammedabdalla8077
    @mohammedabdalla8077 Жыл бұрын

    Nice and very useful

  • @aashnagurjar2477
    @aashnagurjar24776 жыл бұрын

    thnq so much sir

  • @samscience444
    @samscience4445 жыл бұрын

    helpful video thaaaaaank you so much

  • @jeevaniroyal8407
    @jeevaniroyal84075 жыл бұрын

    Gud lecture sir and I have few queries regarding biotechnology

  • @anastaciamodo8273
    @anastaciamodo82735 жыл бұрын

    well presented Shukran

  • @fatimasaleh9301
    @fatimasaleh93015 жыл бұрын

    Love this video

  • @adeelbashir5847
    @adeelbashir58476 жыл бұрын

    nice work sir

  • @niranjanavadivel7388
    @niranjanavadivel73886 жыл бұрын

    Nice explanation Ali Hayek

  • @lamp1215
    @lamp12156 жыл бұрын

    JazakALLAH

  • @noorikhan2974
    @noorikhan29745 жыл бұрын

    Thank u so much sir

  • @Starwave...3821
    @Starwave...38216 жыл бұрын

    Very good video

  • @mustaphaaliyumuhammad4656
    @mustaphaaliyumuhammad46562 жыл бұрын

    thank you sir

  • @shadowwhitehart9190
    @shadowwhitehart91906 жыл бұрын

    Time point 5:33, I think there is a mistake: 0.5 and not 5.0

  • @pseudomonas69
    @pseudomonas696 жыл бұрын

    bruh you deserve an emmy for this vid

  • @abdullahshahnawaz6585
    @abdullahshahnawaz65855 жыл бұрын

    Thank you

  • @khinmyatthu9289
    @khinmyatthu9289 Жыл бұрын

    Hello Dr. Hayek, Thank you very much for this video. The explanation is clear, well-organized, and getting on point, which helps me a lot. I have a few questions. Is there any chemical relation between the concentration of the samples and the incident light emitting or absorbing? I am getting unstable absorbance values with measurements at low concentrations but not at high concentrations. This finding is also beyond the understanding of Absorbance = logarithms of transmission though my values are at 0.1% of T. or could you please enlighten me on the keywords I could find for the articles? Thanks a lot.

  • @AliHayek

    @AliHayek

    Жыл бұрын

    Hi there, Light-matter interaction is a known phenomenon. Sometimes it does not affect the structure of the matter, but some other times light can be aggressive and provides enough energy to break weak bonds. If the absorbance is not stable or keeps changing, it could be that the light is transforming the matter into something that interacts less/more with light. It is a broad topic. I hope this will allow you to start your search. Best of luck.

  • @bharathibharathisekar484
    @bharathibharathisekar4843 жыл бұрын

    Tq,sir

  • @esraatarek953
    @esraatarek9533 жыл бұрын

    Thanks very much for this helpful video. But I wanna ask about the references, please?

  • @Edmerls
    @Edmerls5 жыл бұрын

    Nice

  • @alokneet
    @alokneet6 жыл бұрын

    You are doing excellent job. A little humor will make it perfect. Can you tell me what software you are using for making these videos.

  • @AliHayek

    @AliHayek

    6 жыл бұрын

    +BioMania thank you. I use screenflow

  • @nick1f
    @nick1f7 жыл бұрын

    Is I0 actually the intensity of light as read by detector when a sample of pure solvent (water) is placed in the cuvette?

  • @AliHayek

    @AliHayek

    6 жыл бұрын

    Nick F yes it is indeed. And this is called the blank and it is used for the background noise correction.

  • @ericmanso7355
    @ericmanso73557 жыл бұрын

    nice

  • @yogitavarshney3268
    @yogitavarshney32686 жыл бұрын

    Please upload beer law for mixture

  • @tharunstar1554
    @tharunstar15545 жыл бұрын

    Super

  • @ipekkocaoglu9482
    @ipekkocaoglu94824 жыл бұрын

    Hello the video is wonderful. I can not understand one thing which is when we look the transmittance vs wavelenght graph, why do we observe this shape or when we look the absorption vs wavelenght graph, why do we observe this shape? Thank you so much

  • @AliHayek

    @AliHayek

    4 жыл бұрын

    The best way to think about it is that for the absorption spectrum, the higher the intensity of the peak, the more the light is being absorbed by the solution. Now if you think about it in terms of transmittance. The more the light is absorbed, the less of it will transmit. So transmittance measures how much light is going through the solution. So the lower the peak, the lower the percentage of light transmitted. In a simpler way, the transmittance spectrum is the opposite of the absorption spectrum. I hope it is clear now.

  • @isaiaharchibald4438
    @isaiaharchibald44387 жыл бұрын

    great video but the answer is 5.0 x 10-3 not 5.0 x 10-2. the video really help me thanks btw.

  • @debasishmajhi687

    @debasishmajhi687

    6 жыл бұрын

    WATCHDATFREE u know right that calculators of different types gives different value if u don't know then Google it

  • @kelw5787

    @kelw5787

    6 жыл бұрын

    Yeah I calculated this as well and when I heard it was 5.0 x 10-2 I was confused about what I did wrong xD

  • @rahullovesthepayne8690

    @rahullovesthepayne8690

    6 жыл бұрын

    i thought i was the only one lol

  • @vicentesayson2990
    @vicentesayson29904 жыл бұрын

    Good day sir, is the molar coefficient is a constant given per chemical substance or it's something that we can compute too? I am wondering how am I be able to get the Molar Coefficient of unknown Species and Unknown Concentration, that is present in the water for example lake. This is for spectrophotometer experiment, I can only get the Absorbance and Transmittance but not the Concentration and Molar Coefficient

  • @AliHayek

    @AliHayek

    4 жыл бұрын

    In your case you will not be able to determine the concentration or molar extinction coefficient. You will need one of them known to determine the other.

  • @knowledgejunction1643
    @knowledgejunction16435 жыл бұрын

    Suuuupppppeerrrrr

  • @sanaafreen1937
    @sanaafreen19376 жыл бұрын

    At 2:54 % transmittance is denoted as %T and also in last line as A. Didn't get it. Please explain

  • @AliHayek

    @AliHayek

    6 жыл бұрын

    If you read carefully the last line says that the absorbance (A) can be given in terms of (%T) and the expression is given. I hope this helps.

  • @whats1983
    @whats19832 жыл бұрын

    I am watching from Uzbekistan. How can I contact you?

  • @AliHayek

    @AliHayek

    Жыл бұрын

    Go to www.alihayek.com. 👍

  • @premalathagattu907
    @premalathagattu9073 жыл бұрын

    Sir how find (£)coficiant value Please reply

  • @AliHayek

    @AliHayek

    3 жыл бұрын

    This is explained in details by the end of the video. This is the only way to find epsilon. Good luck.

  • @premalathagattu907

    @premalathagattu907

    3 жыл бұрын

    @@AliHayek thank you sir it is very useful video

  • @kourosh61
    @kourosh617 жыл бұрын

    Answer is 5 x 10^-3

  • @v.c.jayaramaiah4815
    @v.c.jayaramaiah48152 жыл бұрын

    more simple calculation of perceent transmittance or OD vaalues in to Percentage will be more useful for basic chemistry students, videos explainigncalculations msy not be available

  • @PHYSICSINSIGHT
    @PHYSICSINSIGHT4 жыл бұрын

    watch this uv visible spectroscopy is explained very briefly... @

  • @nouralrefahi6000
    @nouralrefahi60005 жыл бұрын

    بس لو بالعربي كان فهمت

  • @alysonbechtum6999
    @alysonbechtum69995 жыл бұрын

    :)

  • @binteabdul6107
    @binteabdul61075 жыл бұрын

    tx for uploading very helpful