Sometimes I think why don't we get professor like you in university? U r like a....... Complete this line friends

@manyamanoj4041

8 ай бұрын

😇

@swagatikapallai1526

3 ай бұрын

Blessing ❤

I'm a student of biotechnology. I found that ur 100time's better than my prof. Thank you sir. May god bless you.

@shomusbiologyofficial

5 жыл бұрын

Glad to hear that you are getting benefit from the videos. Please subscribe and share

@taslimkilledar9359

4 жыл бұрын

Exactly...I am also biotechnology student in msc..

@tanaya_ayarekar

3 жыл бұрын

Exactly 💯

@saranshnigam3896

3 жыл бұрын

I also

@forbii8778

9 ай бұрын

​@@taslimkilledar9359Bro is it worth doing msc in biotechnology??

SAYING "THANK U" WILL NEVER ACTUALLY THANK YOU SIR. YOU HAVE MADE THIS TOPIC CRYSTAL CLEAR TO ME BY YOUR AMAZING TEACHING STYLE. U. ARE. THE. BEST. I NEEDED U. YOU HELPED ME FOR THE PREPARATION OF MY PRESENTATION. U ARE AN ABSOLUTE GREAT TEACHER.I CAN NEVER THANK U ENOUGH EVER!!! LOTS OF LOVE & RESPECT FROM PAKISTAN 💜🌸 , FEELING BEYOND LUCKY TO FIND YOUR CHANNEL.💜😇 YOUR NEW SUBSCRIBER 👉💜👈

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

@BTSARMY-sb8sh

2 жыл бұрын

@@shomusbiologyofficial U are most welcome Sir 💜

You are a machine my friend. I've watched a lot of videos about SB but a clear explanation of the principle of blotting was nowhere to find until I found your video. Thank you for helping me and you have my respect for what you do here. Sharing and explaining the information is an honorable thing to do. I am glad that there are such people as you.

Sir your biology lectures has saved me in my graduation and now they are still a blessing for me in my master's degree.🥺..thankyou so much sir

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

I LOVE your accent. I can easily understand because you point out all the important parts with accent. And also you know what kind of questions listeners would have and you answered all of them. It is like taking a private lesson. Thank you so much! From Korea :)

@shomusbiologyofficial

5 жыл бұрын

Thank you so much for appreciating my efforts

@bishopgorge3578

5 жыл бұрын

His intonation is appreciable!

Thank you sooo much sir for your efforts .... i must say that you are the best biology teacher i ever have

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@baikunthdwivedy5447

2 жыл бұрын

Abhinav goyal sir ka naam suna ha ?

I have an exam on Thursday on this and I never understood this process until I listened to you explain it. Thank you so so much for your help❤️

@shomusbiologyofficial

6 жыл бұрын

Glad to hear that

This man is MASHALLAH :) I've just started watching his tutorials and getting information that I couldn't get during my B.S and M.S. No one can teach better than him. ALLAH bless u

@sarmadghafoor1484

4 жыл бұрын

Welllll....allah is best at everything so your wrong XD

Thank you for making this video. You've explained this way better than my prof!

@shomusbiologyofficial

8 жыл бұрын

+Laura Prince thank you very much.

@sourabhsingh2934

6 жыл бұрын

My teacher finished in 5 min and i was not able to understand😑

This is the first ever lecture that I cried out of satisfaction ! As I have quite being long struggling for this topic .Thank you so much sir.😊😊😊

@shomusbiologyofficial

4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

Very comprehensive. I am a doctor and you have very well explained about all blotting methods.Thank you

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

@normadicn5700

4 жыл бұрын

Same here. He is so on point

@kathmandunepal9547

3 жыл бұрын

I think the lab technicians would know more about blotting than doctors? Because it's literally what they do everyday!

Thank you so much sir I'm graduating just by watching your videos you have saved my life ❤

@shomusbiologyofficial

6 ай бұрын

All the best for future career

Great video! I have Southern Blotting as the subject for my biology presentation, and this is the only thing that helped me understand what Southern Blotting is. Thank you!

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

i have been watching your vids since many years now.. and you have really improved in ur explanations... ur older vids are nice as well.. but the newer ones are even better.. i prefer this format over the older s.blot video!

Whenever i need to clear my doubts i type the topic name in youtube followed by shomu’s biology because literally nobody clears my concepts like he does , he is a blessing sent to us by gods 😭😭

@shomusbiologyofficial

8 ай бұрын

Glad to hear that you're getting benefit from my lectures. Stay tuned for more. Thank you for your appreciation

Finished the video, thanks so much again. Can't stop complimenting you. Such a job has been made... I hope you're continue progressing, evolving.

New subscriber here....I always left out the genetics topics without studing in detail in school, even in puc and degree....but now when I started knowing about genetics in master's degree, your videos helped alot for me to understand the basics and made me to learn and know more about molecular biology and genetics...

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts to help

somus biology is one of the most interesting online class an I can able to get any topic easily in my mind I love this channel in the form of understanding level

You are totally amazing! I find all of you videos interesting and extremely helpful. Thanks God for watching such a great video!!! And thank you for all of you effort too. Student from Thailand.

No professors explained what southern blotting really is. So you are the best professor for me. Thank you 😊 Good luck sir, From Sri Lanka 🇱🇰

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts

Sir you are a savior of my life specially before exam . You teach every portion in a very simplified and lucid way . Always keep us helping like this. Thank you sir.

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

What an excellent way to teach Man!! And covering all the contents in easy way with Full of Interest!!! Shomu Sir hats off to you, You are my inspiration, and i wish i will be as intelligent as you, and explain all topics like you Ameen Tnx for these excellent way to explain topics that makes them interesting.

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

Thank you very very much. There is no such explanation in the youtube even in 2022. May God bless you.

@shomusbiologyofficial

Жыл бұрын

Thank you

I have been watching your vedio since 4 years. Your lessons help me so much to learn clearly about the different branch of biology. Thank you so much sir.

Thanks bro for simplifying such techniques so efficiently..It is far better than those complex contents given in the books....

@shomusbiologyofficial

5 жыл бұрын

Glad you liked my lectures

Thanks Sir doing a incredible job towards learning community irrespective of age, degree or position. Thanks a lot. Sir, u r better than most of teachers, Professor's who don't know how to teach learners. Kudos to u Sir.

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts

again after searching so many sites ,I have reached to the best site .....best explanation

@shomusbiologyofficial

7 жыл бұрын

+chhavi saxena thank you. Glad you liked my lectures. Please subscribe and share

You are a life savior sir! I tried to understand this multiple times for 1and half month couldn't get but now I have got it clearly. U just saved me

@shomusbiologyofficial

5 жыл бұрын

Glad to hear that you are getting benefit from the videos

Your teaching style is best sir 🥰🥰

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts

thank you sir... your video has actually cleared all my concepts... this is the first time when i actually understood the exact meaning of probe n need of using nitro cellulose filter..... thanks a lot sir... god bless u.

@shomusbiologyofficial

7 жыл бұрын

+Meenu Garg thank you. Glad it helped.

it took more time to read d same thing from book but just some minutes r required to understand d same thing in more clear way from your lectures...the way u taught d thing and make us learnt is really awesome.

The video made me understand southern blotting very easily. Off to watch northern blotting! Looking forward to more of your content!

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

thank you very much. i have my exams the day after tomorrow and this really helped.

Denaturation of fragmented dsDNA is done before transferring to NItro cellulose membrane. Otherwise how this DNA will attach to the membrane.

@batkuparpohtam8070

4 жыл бұрын

U r right it should have done before tranfering into nitogenous paper

@saneha7020

3 жыл бұрын

Hey can you tell me why those ss dna dont bind to each other again before binding to probe ???

Excelent explanation! Very easy to follow and very clear. Thank you so much!

You are the best biology teacher. Thank you so much sir. Before all of my exams, your videos always give me a crystal clear concept.

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

I am a medical student and you explained it so well. Thank you

@shomusbiologyofficial

3 жыл бұрын

You're welcome

Amazing teacher..... Thank you soo much sir...and respect u a lot.....

@shomusbiologyofficial

7 жыл бұрын

+Amrita Rathore thank you. Glad you liked my lectures

You have so much knowledge and the best thing is you know how to share it as well, keep up the good great.. you're really amazing 💎💎

Awesome video :) Keep up the great work! Im a doctor and I love teaching too, and your videos show me how to be a better teacher. Cheers!

i regret why didn't i find it before... thanks a lot

Mr Shomu I had a presentation for university about Southern blot , I didn't get it at all before but ur video literally was satisfying and amazing, thank u sooo much man , I did a rly nice presentation today all based on your video with the same way of explication ❤

@shomusbiologyofficial

9 ай бұрын

Glad to hear that you're getting benefit from my lectures

I have check severals youtube video looking for some tutoral for my master in microbiology, as a physician i find this concepts very difficult to understand, I am a teacher myself i can notice this is pretty good explaining material you just dont explain the technique itself you relate everything in a way that is easy to learn and you also explain for what is gonna get usefull. Kudos to you. Wish me an approval in my exam

@shomusbiologyofficial

Жыл бұрын

Glad to help you out. All the best 👍

excellant video... even books also dont explain this well. thanks...

@shomusbiologyofficial

8 жыл бұрын

+indranil bhattacharjee thank you very much. Glad you liked this lecture.

Love the way of your teaching..😊😊😊

Thank u sir for getting concepts clear within minutes..

Thank you so much.. for helping us such an effective way... how you explain its great to watch. I love to watch your videos it is helping me a lot.

Wow! Best explanation ever. I love your videos. They are so easy to understand and it makes the subject more interesting. Thank you for your efforts sir.

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts

@ChrististhereasonLecheKoza

3 жыл бұрын

@@shomusbiologyofficial Welcome sir

Sir your lectures are awesome. Thanks for your such effort.

@shomusbiologyofficial

7 жыл бұрын

+Amisha Patel glad to hear that

Southern blotting was a foreign concept to me before I watched this video. Thank you, Shamu....as usual!

Thank you, this helped me a lot, and it was explained very well.

an amazing video!!!!! thank you very much!!!!

@shomusbiologyofficial

7 жыл бұрын

+Joanna Przybyszewska glad you liked my lectures

U r awsm sir I am going to see ur all lecture ...to crack CSIR..I need this way of lecture to understand 😊 THANK YOU SO MUCH

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

what a great energy you have! you have really helped me to understand these otherwise abstract concepts

@shomusbiologyofficial

7 жыл бұрын

+Heather van Wolf thank you. Glad you liked my lectures

This video was so helpful! It was the best explanation on Southern blotting I have ever seen. Thank you so much!

@shomusbiologyofficial

9 ай бұрын

Glad to hear that you're getting benefit from my lectures

I know I'm late but I have an exam tomorrow (Btech Biotechnology) and this is simply the best video on Southern Blotting on KZread. Not only the content but the enthusiasm with which you explain is so good to see that I understood everything and am left with a smile on my face. Hats off to you sir!

@shomusbiologyofficial

Жыл бұрын

Thank you so much for appreciating my efforts. Glad you liked it

Thank you soo much sir...I love Ur passion about teaching..

@shomusbiologyofficial

6 жыл бұрын

Glad to hear that

I had a chemistry exam today at uni and now i am studying for a molec genetics exam for tmrw and i just wanted to say that god may bless him. 7 yeqrs later and he has helped countless of students.

@shomusbiologyofficial

8 ай бұрын

Thank you so much for appreciating my efforts. Stay tuned for more

Sir you teach very will and the best method of teaching very helpful best teacher from the youtube great sir keep making videos we will be supporting you Brother

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

☺️ that's yu sir... ever i get understood all d concept nd i feel Gud fr it... 💯 teaching no error... love wd teaching... untill i saw ur vd i throught wt a sucking topic.. but now im gud wd it... Can score Gud ❤❤ 💯... wish u to upload more

@shomusbiologyofficial

4 жыл бұрын

Glad you liked my lectures

@reshmimishra648

4 жыл бұрын

😊😊.. thanku sooo much 💓.. just xam got over wrote Gud

Thank you so much sir❤ your way of teaching is very understandable..ALLAH pak bless you🤲

@shomusbiologyofficial

Жыл бұрын

Thank you

Sir when i search my topic on you tube and i see ur video of that topic my tension becomes half becoz u r great and your videos are too much helpful thank you so much sir😊

@shomusbiologyofficial

4 жыл бұрын

Thank you. Glad you liked my lectures

u r that friend who helps us during xams by explaining everything for a person like me who is a visual thinker. thanx a ton buddy

@shomusbiologyofficial

7 жыл бұрын

+Ann Martin thank you. Glad you liked my lectures

You are the best teacher! I am doing undergrad in Biotechnology and class lectures and slides exhaust me. You are the one who is making the graduation process a little less harder! Respect.

@shomusbiologyofficial

Жыл бұрын

Thank you so much for appreciating my efforts

please keep doing these stuffs

@shomusbiologyofficial

7 жыл бұрын

+shivam jar thank you. Glad you liked my lectures

Thank you for making this video. I stumbled on it today and I am definitely subscribing.

@shomusbiologyofficial

Ай бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

Ur super duper a1 .... ur way of teaching is very easy... u usually don't left any questions in our minds we got whole fully info about our desire topics...

Great man

Wow what a lecture..♥️

@shomusbiologyofficial

Жыл бұрын

Thank you

Hello Shomu Bhattacharjee ! I'm a 2nd year student who had to deliver seminar about the same topic .The whole concept of SBT has been told by you in a very easy and understandable manner , you video has been very beneficial for me , Thanking you for the same .

@shomusbiologyofficial

Жыл бұрын

Glad it helped

Amazing as always!! Thanks for sharing and keep up the great job!!

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked the lecture

U are a best teacher ever found ❤❤

@shomusbiologyofficial

Жыл бұрын

Thank you. I am honoured

Why do you need alkaline buffer? What would happen if I used acidic buffer, water or Histidine? Why does it HAVE to be alkaline buffer for the imprint to work?

@jakkuwolfinsomnia8058

7 жыл бұрын

Thank you ^.^ I understand now

@kristinwade344

5 жыл бұрын

dna is negatively charged(phosphate group). alkaline buffer is more basic (ready to share an H+) and acidic buffer is ready to take an H+. in this reaction dna is the acid and is reacting with the basic buffer... this is my thinking..and reference from my orgo chem class... i think the phosphate group which consists of P(-)H3, where the phosphate atom is negative, would give away other H if combined with something more acidic or some compound wanting a H more. Causing the dna to become more reactive.

@mellamopepe619

5 жыл бұрын

Acids contain excess H+ ions, which bind quickly to the phosphate (PO4)- backbone and cancel out the negative charge. Without charge, DNA can't migrate because it won't be attracted to the (+) electrode. The alkaline buffer does the opposite, neutralizing any H+ (or Ca2+, Mg2+, or other + ion) by pulling them off the DNA, into solution. Alkaline means excess OH-, so by dissolving any (+) ions, it makes sure that the DNA will be (-) and be attracted to the positive electrode (allowing migration and development of bands).

স্যার আপনি অনেক ভালো করে conceptual বোঝান এতে আমার অনেক প্রবলেম গুলো clear হয়ে যায়।। thank u sir

@shomusbiologyofficial

3 жыл бұрын

Dhonyobaad

Beautifully explained!! thank-you!!

Sir I am have a doubt. How do we know that the DNA of the required sequence is 100% present? Cause we have prepared complementary probe of that sequence right? How do we know that's sequence is present? :/ Also we can do sequencing to know whether it's there or not? I just don't understand. And also sir can you please explain regarding restriction mapping and restriction digestion. Cause I've watch all of those videos but you haven't mentioned about the partial digest double digest and all. And no one can explain better than you its a requesttttttttttt. And i am in th ir d year biotechnology can you suggest reference books for RECOMBINANT DNA TECHNOLOGY.

@malikmudasir3568

5 жыл бұрын

actually as far as i know we do sB in order to confirm the gene of interest. we have gene banks where we have sequenced the genome and we make the probes against the segments whose secquuences we have already done and because of hybridization technique ie probes we can diagnose

@malikmudasir3568

5 жыл бұрын

I hope u have got it this fanda is diagnosis ☺️

But what if the restriction endonuclease (DNase I ) cuts in between the target DNA , Then how our probe will bind to target DNA?

@tanyaguduru2606

7 жыл бұрын

megha sharma the restriction endonuclease cuts in specific places only. If you know the sequence of the target dna then you can control which endonuclease to use to cut in what location.

@julianunez7489

6 жыл бұрын

creo que la enzima de restricción solo corta en ciertas partes.

Very nice explanation. Very much easy to understand. Thanks

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

love ur video mate, keep it up. You are a better teacher than my biology teacher!

Don't we use nylon membrane in Southern Blotting and nitrocellulose in Western Blotting? Im confused..

@shadowxxrami6669

7 жыл бұрын

Aishwarya D my same initial thought . can anybody explain ? I'm confused

@jeevankishore8196

7 жыл бұрын

Aishwarya D either nitrocellulose or nylon membrane can be used in both the technique .

@ner0xic537

6 жыл бұрын

both can be used

You have made a great explanation sir.Your technique of teaching is very effective that clears many concepts. Thank you so much.

@shomusbiologyofficial

4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

Today i learnt southren blotting by this video... 😁😍 Yesterday i learnt Western blotting. Hats off for uploading these type of informative lectures.

@shomusbiologyofficial

6 жыл бұрын

+Ganwal Byanka glad to hear that

Excellent explanation! Thank you~

Your lecture are easy to understand and you are amazing 👏

@shomusbiologyofficial

7 ай бұрын

Thank you

Your lectures are so much concept clearing sir Two doubts i have from this lecture 1. How to place the gel on the buffer as it is fragile?? 2. How to confirm that all the DNA fragments from gel had migrated to the nitrocellulose paper?? Thank you for clearing concepts of me and many lakhs of students

awesome explanation which is not in our practical classes.. thank you very much..I love you man..

I think there is no other person who explains like you in a simpler way Even though it is complicated.....

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

Thank you shomu. You are the GOAT

This the mixture of electrophoresis and pcr😅😅😅 at the middle the blotting done...thank u so much sir... great explanation of each and every steps❤❤❤

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

Thank you so much sir.. your very talented and u can teach very clearly and simply.. Better than some prof.s Well done good job.. Again THANK YOU SOO MUCH..!!!

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts. Stay tuned for more

Your video is so much helpful for better understanding I clearly understood southern blotting from Ur video thank you 😊 so much

Thank you so much you're brilliant! You did a great job!🌼

@shomusbiologyofficial

5 жыл бұрын

Thabk you. Glad you liked my lecture

Thank you so much...You explained very well...It really helps me to understand the whole process thoroughly...😊 But the only thing that I think you miss to tell is the washings of the membrane filter which is important for removal of unwanted stuff....

Thankyou for helping us. May God bless u.

Thank u so much.... Really.. I was so confused with it... But now it's clear.. Ur the best 👍💯..

Thank you Dr. Shomus you are amazing.

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

Thank you sir soo much due to this lock down I was not able to understand blotting in online lectures from my college but now you have completely made me understand.

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

That video was awesome. Gave full understanding. Keep up good work brother

@shomusbiologyofficial

Жыл бұрын

You're welcome

Easy to understand after your explanation sir. Thank you🙏