Southern Blot

Ғылым және технология

( www.abnova.com ) - The Southern blot is used to detect a specific DNA sequence in DNA samples. A general blotting procedure starts with the separation of digested DNA fragments. The DNA can then be transferred to a nylon membrane through a capillary system and detected with a hybridization probe. More videos at Abnova www.abnova.com

Пікірлер: 54

  • @HimanshuSharma-oy9ss
    @HimanshuSharma-oy9ss3 жыл бұрын

    I just came back for the background music.

  • @asiafernandez1345

    @asiafernandez1345

    6 ай бұрын

    Lmao no lie

  • @abduraheemt5735
    @abduraheemt57356 жыл бұрын

    Nice video. Pls show the coloured DNA fragments too.

  • @Aurelius787
    @Aurelius78711 жыл бұрын

    catchy beats yo

  • @shelby_da_great
    @shelby_da_great Жыл бұрын

    Ngl I enjoyed the background music

  • @anumqureshy9831
    @anumqureshy98313 жыл бұрын

    Nice video! Great experience!

  • @liptikantabhoi7665
    @liptikantabhoi7665 Жыл бұрын

    Great practical sir/Ma'am. Really helpful for practical hand.

  • @docjrae
    @docjrae12 жыл бұрын

    Can you show the results please?

  • @lovelyperson9
    @lovelyperson98 жыл бұрын

    Nice video. Also, I like the choice of music : ]

  • @lusubilomalakbungu6032
    @lusubilomalakbungu60328 жыл бұрын

    Is there no addition of Detection buffer at last?Am curious to see the results for this.

  • @luntuyen8381
    @luntuyen83817 жыл бұрын

    what is hybridization probe of abnova that i can use to do like this video

  • @darleneestrada8101
    @darleneestrada81018 жыл бұрын

    This technique was the precursor to much fancier technology now called micro arrays...Thank you Edward Southern.

  • @emilyr6390
    @emilyr63902 жыл бұрын

    To be perfect just needed to show the results at the end

  • @priyadarshini321
    @priyadarshini3214 жыл бұрын

    I love experiments this video is useful for all the students ...

  • @A2Kaid
    @A2Kaid11 жыл бұрын

    Yes I used gel electrophoresis for proteins.

  • @mohanarangan.r9567
    @mohanarangan.r95675 жыл бұрын

    thank you too

  • @moistschmeckles400
    @moistschmeckles40017 күн бұрын

    Background music on 1,5x is something else

  • @forbesavila8827
    @forbesavila88274 жыл бұрын

    A bit curious since there are quite a good educational youtube video, is there a way to cite them in a paper lol?

  • @vovaparkourukraine1
    @vovaparkourukraine16 жыл бұрын

    I don't even know this shit but this was a great video

  • @abdulabdullahi7575
    @abdulabdullahi75758 жыл бұрын

    After blotting What next

  • @hollyredman-peat3869
    @hollyredman-peat38695 жыл бұрын

    where is this song from!!!??

  • @Alex456Ortiz
    @Alex456Ortiz12 жыл бұрын

    What happens next!!!!

  • @zx16381
    @zx1638111 жыл бұрын

    gel electrophoresis can't be used for protiens SDS PAGE is used for proiens

  • @sciencetrainee3583
    @sciencetrainee35834 жыл бұрын

    Wow, this is awesome! Makes sense after watching doing it in practice. I would like to try it out... Though, it seems like it is a very long procedure? Several days to finish?

  • @christopherotis133

    @christopherotis133

    2 жыл бұрын

    you probably dont care but does anybody know a way to get back into an instagram account?? I was stupid lost my login password. I would appreciate any help you can offer me.

  • @apollolane8879

    @apollolane8879

    2 жыл бұрын

    @Christopher Otis instablaster ;)

  • @christopherotis133

    @christopherotis133

    2 жыл бұрын

    @Apollo Lane Thanks for your reply. I got to the site through google and Im in the hacking process now. Takes a while so I will reply here later with my results.

  • @christopherotis133

    @christopherotis133

    2 жыл бұрын

    @Apollo Lane It worked and I finally got access to my account again. I am so happy:D Thanks so much, you saved my account :D

  • @apollolane8879

    @apollolane8879

    2 жыл бұрын

    @Christopher Otis Glad I could help :D

  • @hafsashahzad8072
    @hafsashahzad80722 жыл бұрын

    Why is NaCl used in alkaline and neutralization buffers? How does NaCl play role in dna denaturation? Will neutralizing the solution not rejoin the dna strands?

  • @tettehjamesnartey548

    @tettehjamesnartey548

    Ай бұрын

    NaCl in alkaline and neutralization buffers increases ionic strength, aids in protein precipitation, and stabilizes DNA in solution. NaCl stabilizes the DNA double helix, making it more resistant to denaturation in high salt conditions. Neutralizing the solution can allow for reannealing of DNA strands, but the efficiency depends on DNA size, concentration, and buffer conditions. During plasmid isolation, plasmid DNA reanneals more efficiently due to its smaller size and supercoiled nature compared to fragmented genomic DNA

  • @zyfnic
    @zyfnic12 жыл бұрын

    what a good video for southern blot. By the way, Chinese lab is so kind to public their techniques to the whole world for free of charge. It is kind of the lab.

  • @abdulabdullahi7575
    @abdulabdullahi75758 жыл бұрын

    After autoradiagrahy What nex

  • @thinhhua7638
    @thinhhua76385 жыл бұрын

    How do I find the song in the video? The description doesn't help me find anything

  • @aches6652
    @aches66529 жыл бұрын

    what next :( ?

  • @abcdefghi9
    @abcdefghi912 жыл бұрын

    all dat and i still seein a blank piece of paper????????/

  • @sukhchain9696

    @sukhchain9696

    3 жыл бұрын

    *cough* autoradioscopy *cough*

  • @galvanchristian
    @galvanchristian12 жыл бұрын

    @spongenooob I agree!! :D

  • @blindsurgeonc
    @blindsurgeonc9 жыл бұрын

    Gosh! Sooo long

  • @A2Kaid
    @A2Kaid11 жыл бұрын

    I did this with proteins...

  • @sciencetrainee3583

    @sciencetrainee3583

    4 жыл бұрын

    Western blot? :D

  • @auroraxxx7828
    @auroraxxx78284 жыл бұрын

    online labbing in 2020;(

  • @ranodipkanshabanik4832
    @ranodipkanshabanik4832 Жыл бұрын

    🙂🙂🙂🙂

  • @sinthy8447
    @sinthy84474 жыл бұрын

    কিচ্ছু বাল ই বুঝি না😑😑😑

  • @swiss4ever93
    @swiss4ever934 жыл бұрын

    Science is still so primitive, all this just to see DNA??? and we wanna fly to mars🤣🤣

  • @sukhchain9696

    @sukhchain9696

    3 жыл бұрын

    maybe you should know about about the basics before commenting on it and this technique is not what is used these days.......Real-Time Quantitative PCR is used now, which you may notice is how covid is detected

  • @swiss4ever93

    @swiss4ever93

    3 жыл бұрын

    @@sukhchain9696 and we're doing so great with covid, thanks for emphasizing my point

  • @sukhchain9696

    @sukhchain9696

    3 жыл бұрын

    @@swiss4ever93 Well we are primitive but not in the way you are thinking. Don't trust me just start searching on the internet about the underlying principles of these very primitive technologies you just complimented.........

  • @lumieredice485

    @lumieredice485

    3 ай бұрын

    @@sukhchain9696 interesting...so my class is basically giving out outdated information and links...

  • @sukhchain9696

    @sukhchain9696

    3 ай бұрын

    @@lumieredice485 I wouldn't say outdated, I am sure there are many specific tasks it is still used for, I just don't know. Maybe It's the same as how they teach us all the atomic theories like Thomas, Rutherford, Bohr's atomic models before moving to what is more acceptable today. Some prior easier, maybe outdated/faulty stuff can help in teaching the stuff that's more advanced. A bit of history lesson hidden in all the science to honor those who spent their life for it. Or the fact that we are not ready yet to understand the more advanced voodoo magic. Maybe a little bit of everything, maybe neither of it and I am just rambling nonsense. I don't know 🤷‍♀, I just like talking too much.

  • @gabyzukata1901
    @gabyzukata190112 жыл бұрын

    An okay attempt at a video, but it's sooo boring and amateurish. I'll try to remember to avoid Abnova when I need reagents for an experiment whose data I'm going to publish publically....

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