Promoter and Termination Sites of Transcription
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@hibahaitem7878
2 жыл бұрын
I agree one hundred percent
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@AKLECTURES
8 жыл бұрын
+Arosya haha! thanks for the kind words, great job on your recent exam!
@hibahaitem7878
2 жыл бұрын
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very well explained video, even for forein people! It will make me pass my biochemistry exam in one week :) Greetings from Germany!
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Hat off to you sir. I bet many people will find in these videos the enthusiasm and skill to teach that many times is kacking in their classrooms. Brilliant performance, :)
@AKLECTURES
9 жыл бұрын
Red Orr thank you for the kind words! :)
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@AKLECTURES
9 жыл бұрын
Dayna B Thank you for your kind words Danya!
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excellent explanation. you have made it simple to understand
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+AK LECTURES I think you should distribute this video into two, one for prokaryotes while another for eukaryotes. Thank you very much for your lecture! I really appreciated it! #bigfanfromyou
You are Fantastic
Awesome expalanatin..thank you sir..
@AKLECTURES
9 жыл бұрын
neha verma you're welcome :)
It is written DNA template, isn't it nontemplate stand? Actually 3' to 5' one is template I guess.
감사합니다
sir you are the best
thank you soo much!!
CAAT box sequence is wrongly mentioned as GGNCAATCT,rather than GGCCAATCT !
You are a saint
Genius!
Sir i follow Biochemistry Book by Donald Voet and Judith Voet . I found lot many details in this book do you have videous that talks about these details ... I found your videous very helpfull .. Thanks for nice lectures.. :)
3:30 There is a mistake in the video on the picture there is not a DNA template strand but CODING strand because mRNA is made in 5'-3' end direction and all the names on the DNA strand are given as are on codding strand of DNA not template.
@JongensJavaanse
6 жыл бұрын
It is written wrong but he is explaining it correctly
@issackabdi6946
5 жыл бұрын
Sure
@issackabdi6946
5 жыл бұрын
J
Really cool video! But I'd like to mention, that when you describe CAAT box for eucariotic cells, it is written on the board, that it is GGNCAATCT, which can not be true, because there is no nucliotide N. According to wikipedia, it starts as GGCCAATCT (N is changed to C).
@SixTough
4 жыл бұрын
N stands for anything
Do most eukaryotic transcriptions tend to separate via termination by a stem-loop signaling, or is more thanks to the function of RNA helicase?
Hi thank you for all your videos. Do you have more please especially on biochemistry
@AKLECTURES
Жыл бұрын
check out the website in the description.
Says DNA template, however isn't the DNA template strand the complement of RNA transcript? It should be the coding strand, because it is not the strand that is read.
Super,thanks! A stupid question but how does enhancer sequence just bind to the site as a promoter? Does the DNA just bind to itself? How does it get there?
On the whiteboard is a diagram of 5’ to 3’ DNA with the pribnow promoter box shown to the right of the 5’ end of a DNA single strand. Yet you say it is the compliment of this strand that is read by the RNA polymerase starting at the 3’ end of the not shown “template” strand. If so, then what is the nucleotide sequence for our pribnow promoter site? Shouldn’t the nucleotide sequence of pribnow be the compliment of the shown TATAAT? Which is really ATATTA? Can you see the dilemma here?
bless you
pefect.....thank you :)
@AKLECTURES
9 жыл бұрын
Mohamed Emad You're welcome!
Great video! Thanks! Quick question. Is 35 and 25 distance from transcription in prokaryotes and 1000, 75 and 25 in eukaryotes hard distances, are they an exact average, or are they rough approximations?
@chooyuanchun4836
6 жыл бұрын
They are only rough approximations because it might differs from different individual not every individual having the same amount of nucleotides in a certain strand of DNA hence when it was being transcript then the amount differs too. For approximations of -35 and -10 nucleotides upstream sequence are for prokaryotes while in eukaryotes are -25 to -35 nucleotides upstream sequence ( these are all just rough approximations ) nobody can give you the exact place for every individual as I mentioned it differs. :)
Question Tata box sequence is located on template strand or coding strand ???
Pls sir, the strand the promoter regions are on, is it on the 5' to 3' or on the 3' to 5' . Thanks so much
I was watching your video on eukaryotic gene structure and there you said the RNA Pol II binds to the transcription start site. Now in this video you said it binds to the promoter. im confused....
this video is not inside one of your playlists, is it? If it is, I want to see that playlist!
He literally prints a whole guide on his white board.
which lecture involves the rho?
Well that’s it, this is how I pass Cell Biology.
*looks at top right corner of whiteboard* - Are all eukaryotic genes called Eugene? hehe. Thank you once again!
do you have an RNA transcription for eukaryotes??
@misbahashraf9485
8 жыл бұрын
+Jonathan Weiss i guess so yes.. and its promoter sequence ranges from -25 and -75. and its somewhat obvious if there wont be any transcription process then how ur body would be able to produce proteins, as we know that RNA helps in protein synthesis. I hope m clear to you.
Can i just know ,what does N stands in CAAT box please.
am so confused where is the promoter located is it on the sense dna or what plz answer me
You are incredibly a nice teacher, kindly improve on your conclusive remarks.
Sir can you please give lecture about translation
why transcription factors are in eukaryotic transcription but not in prokaryotes?
@nomsahabeenzu8219
4 жыл бұрын
Woooooooooow awesome 👌 💕 👌 well explained, thanks so much
hello sir, actually i didn't get what do u mean by bacterial gene>? i mean i know what is gene but how transcription can start from -10 units or -35 units from bacterial gene? i just didn't get that point. :D @aklectures thanks for delivering worthy lectures!
@jamesfilosa6277
8 жыл бұрын
+Misbah Ashraf - I think the answer here is that RNA polymerase spans a large region of DNA before the gene. The core enzyme has a mass of hundreds of kDa... So the complex is large enough, and has enough active sites, to recognise promoter sites. Transcription does not start at these sites. Transcription starts at 0. -10 and -35 are in relation to transcription start.
@Iyami001
8 жыл бұрын
+Misbah Ashraf The promoter region is just where RNA Polymerase will bind to the template. It does not actually start the transcription process until it gets to the start codon nearby it.
Why do has the mRNA of prokaryotes no promoter?
What does the N denote in the CAAT box
@holohedx2
8 жыл бұрын
+Cognitive Philosophy Don't quote me on this, but I think it just means nucleotide, as in any base can go there.
@MagicTurdd
8 жыл бұрын
+holohedx2 yes it does
why can't you be my biochem professor :(
LOL we look alike, a little bit. only 99.99999999% identical.
boss