You rock man. Normally you go to class to help you understand your book. I'm finding when I'm in class I can't understand my teacher at all, then I end up going to the book to try and get some clarity, which hardly ever happens. All the money I'm paying my college, and basically KZread is the one doing the teaching.

@g-mannG

4 жыл бұрын

Same here! At least in Biochemistry.

You deserve a lot more attention, your knowledge and explanations are amazing. Thanks so much!

14 minutes worth a 2 hrs lecture .THANK YOU 💗💗

I can always count on your lectures to understand any confusing topic in class. Thank you

@AKLECTURES

9 жыл бұрын

thats wonderful to hear! you're welcome :)

Thank you so much AK lectures! You have actually helped me pass my semesters wonderfully well. Please keep posting more and more videos like these. Its been 2 years now that I can actually count on your videos instead of the text. Thanks a lot man!

This video deserved at least a mil views, really awesome presented!

Every thing I want involved in molecular biology I visit your channel because you have answer to all my questions Thanks alot

Been Studying Replication for the past 2 hours looking at notes/and lecture slides. I just need to watch this 14 minute video and a light turns on. This is why I've donated and will continue to.

Your explanations are so clear! I always come out of your lectures really feeling confident about the material!

I could never understand the rationale behind lagging strands, this helped a lot. Thank you

I can't beleive we are blessed with free content like this thank you so much!

Finally understood the DNA replication thank you!

AK ...you are my teacher currently am a student in Kenyan university of mmust ....l love your work ...keep on moving this way ..I love you

This was amazing. I dont really have basic knowledge on DNA but i undertood everthing and you speak very clearly. Thank you.

I will donating money to this cause until I no longer exist on Earth. You are great and appreciated deeply. Thank for your knowledge!

Hi Andrey, I scrolled and scrolled to find your class because I couldn't remind your name. I like your lectures

Just want to add some details. DNApolymerase has 3 subunits. One is reserved for leading while two is for lagging strand. Imagine a person upside down walking backwards towards replication fork where the head synthesise the leading strand while the two legs synthesise the lagging strand. Also the lagging parental strand is twisted inwards for ease of replication. Source : Watson’s Molecular Biology of Cell.

Amazing as usual. You are undoubtedly the best!

AK Lectures "On point", very detailed explanation and I understand it! Thank you!!

always clear always helpful... i prefer your lectures over hundreds of other...

Im very lucky to have found you, thank you

Thank you so much for demonstrating such hard topics and make it easier for us to understand, thank you from the bottom of my heart for your efforts,god bless you

Honestly bless ur soul, I have a AP biology exam tmw and I thought I was gonna fail

Your videos are so good! I owe you at least 10% of my final degree classification :) thanks

@AKLECTURES

9 жыл бұрын

Simon B hah! thats pretty sweet! you're welcome, use your degree wisely!

Your lectures are always awesome

Execellent work i understand more your lecture than any other

thank you so much , you always make it easier .

thanks sir ur brilliant teacher

he is great sometimes speak to quick but keep the line, very well he enjoys, and sometime he enter in a kind of trance very delightful that makes people to understand,

Excellent lecture !! Thank You so muuuuuuuuuuuuuch !

this is sooooo good!!

u make work simplier its even great that an average of lecture hours in university would tke weeks to complete a sub topic u good at it,,,,lov yah

everything now make a perfect scence thank u so much u are a real star

The only thing that you missed here, is the removal of the primer before DNA ligase comes in and seals the DNA molecules together. Great Vide!

@noonoo97204

6 жыл бұрын

Diego I Ortiz Mendiguren Hi, do you know what enzyme removes the primers please?

@gunfighter3890

6 жыл бұрын

Nayla Nayab Flap Endonuclease 1 (FEN1)

@michaelolafitness9491

5 жыл бұрын

Nayla Nayab aren’t they called Okazaki fragments not primerse

@michaelolafitness9491

5 жыл бұрын

?

wonderful work

Thanks for the lesson

Truly, I love you man 🤩🤩🤩🤩

Thank u . your way of teaching is very good. people easy know it

thank you for this video!

this is amazing 🔥

+aklectures awesome video, i"ve been watching a lot of your video to help me in my exam, so I owe you a loottt,,, thank you,,, however this in this video you didn't mention anything about proofreading and 3' -> 5' exonuclease nor any other exo/endonuclease. do you make a separate video to discuss this?

you drawing the leading and lagging strands while explaining just made me realize why the lagging strand is called as it is. It's because the lagging strand/Okazaki fragments grow in the opposite direction of the moving replication fork. Therefore, there needs to be at least some space or room for the primosomes to attach itself first before the individual addition of nucleotides can occur. This need does not arise for the leading strand because there is always room/space made by the unzipping of the helicase. Could the lagging strand's mode of polymerization be the reason why it forms in fragments?

@christine2689

3 жыл бұрын

Just watched. Yes, it is the reason. So that the Okazaki fragments can grow at about the same time, there have to be multiple initial replication points in which the polymerization can then proceed. Does this mean that the lagging strand requires more RNA primers than the leading strand?

@christine2689

3 жыл бұрын

Just watched. Yes, the RNA primase creates more RNA primers for the lagging strand.

@GeneticsLessons

3 жыл бұрын

Yes because replication goes in two direction but DNA polymerase only can ad new nucleotides in 5'-3' direction only.

Thanks a lot !

I m french and i dont know lot of english but your videos are really better than my lessons

I love your explination

Thank you!!!

You are amazing! It is so easy to understand when you explain it. Can I ask about your background? Are you a lecturer? Thank you so much for your lectures.

Very much informative and we'll narrated

Very helpful..thanks alot

Thanks!!

thank u so much for ur lecture

you are truly amaziiiiinnnnnnnggggggg

Thank you so much🙏🏻

Hi, thank you very much for your helpful video. I got the impression from your video that the DNA helicase only moves in one direction but I thought that the replication forks move in opposite directions opening up the DNA on both sides of the replication origin. Could you please clarify this for me.

you are the best

thanks doctor ⚘☘

So good

its very helpful thanks

amazing! Ty

Thank you sir so much !!!!

thanks for help me to uderstad this lesson

Super awesome

great video. is there a video with Telomere function explanation?

thanks now i know what the difference between the lagging and the leading strand

@AKLECTURES

9 жыл бұрын

salma awesome! you're welcome :)

@neomcyfolau2126

5 жыл бұрын

this is cool thank you

Very nice

Hello, I have a remark: I guess there are two helicases per "opened bubble" at work. The point of origin is in the center of a strand that belongs to a bubble.

+ak lectures i really love your lectures its helped me greatly premed student here

@AKLECTURES

8 жыл бұрын

+Shane Savage awesome, keep up the hard work! :)

@shanesavage484

8 жыл бұрын

what do you have your degree in if you dont mind me asking

@AKLECTURES

8 жыл бұрын

+Shane Savage Physics and mathematics and I am working on my MD.

Video content may need to be updated. I think from what I learned from B. subtilis experiment, the origin should start from the middle where the DNA primase is binded and then, half the opening on the 5' to 3' strand on top become the leading strand. And then, DNA polymerase start to make daughter strand, the on the right of the origin in middle, lagging strand start to fill in the gap. Furthermore, the bottom strand start to have okazaki fragments to fill in nucleotides for bottom lagging strands. The part I think this video might be wrong is the origin of where DNA primase should be in the middle of the gap and not the right side of the gap, which is made by helicase. Thank you for correcting me if I am wrong

@henryche121212

7 жыл бұрын

Here is one of my sources: biology.stackexchange.com/questions/31585/does-dna-polymerase-always-go-the-same-direction/31590

@maka55567

3 жыл бұрын

Bacteria have circular chromosomes which is why it’s that way. Ak explained a linear DNA replication so clear your misconceptions.

why should we use only T radiocatively labelled T? is it to measure only replication and not transcription?

HI! is this the DNA-replication that takes place in the human cells or in the bacterial cells?

cooool!!!!!!!!!..... thank yu.......

thank you so much, literally the best explainer😊

Is DNA gyrase and topoisomerase same?

u rock tnx a lot lot

Beautiful lecture The supercoiling you allude to from the removal of the hydrogen bond the biochemical scaffolding as a phosphodiester call the call the double helix tracks Enabling nucleotides hydrogen bonded as complementary pairs to be separated with the helicase gyrase process I'm sure you agree the magic is in the DNA sequence you have the rail the scaffolding down really well but what I'm unsure of the helicase is supercoiling to compensate the removal of the hydrogen bond the lattice wants to wind you say i say spin, as a positive supercoiling, you say again negative supercoiling introduced by the gyrase enzyme Supercoiling that might be a descriptive but what reference point because the next word is negative supercoiling just as a curiosity looking at these molecules is it left or right hand spin? or spiral? anybody know when the DNA is intact with the phosphorus bond and the hydrogen bond? creating a structural lattice the scaffolding with a phosphorus backbone Does the double-stranded unit does it have a propensity to spin left-handed or right-handed using the electron flow physics terminology of Elementary particle electron spin? Left or right hand spin please?

in the lagging strand, why don't we add the RNA primer to the 3' end and we continue adding DNA peacefully? since it is bidirectional synthesis. I think we will get 5'-3' DNA without those fragments. Isn't it?

@alvinlee2522

3 жыл бұрын

EXACTLY! I’VE BEEN WATCHING SO MANY VIDEOS AND READING SO MANY WEBSITES BUT NOTHING IS ANSWERING THIS QUESTION

thanks af

can you mention me feature of d n a replication

🙏

Flap Endonuclease 1 (FEN 1)?

Is it so that every Okazaki fragment consists of one RNA-primer (one RNA-nucleotid) and many DNA-nukleotides???

@GeneticsLessons

3 жыл бұрын

No this is not the only difference between DNA and RNA - in RNA sugar is ribose and in DNA deoxyribose - so A, G, C nucleotides in RNA and DNA are not the same.

YOURE AMAZING THANK YOU

damn, this guy is in the freakin' zone

13:30 full-screen lecture

Supper star

Mistake present in your description of lagging strand.

So this is what dna replication actually looks like 🙃

😍😍😍😍😍😍😍😘😘😘😘😘😘😘🤩🤩🤩🤩🤩🤩

Lmao same man same

I should be paying you my 9K instead of university.

thanks sir ur brilliant teacher