Thank you, it might sound crazy but watching you do that has finally given me the confidence to pour my own plates. I appreciate you very much!

@edwardgrand

6 ай бұрын

It isn't too hard. Some will inevitably get contaminated. Don't worry. It's the door to all other advanced techniques.

So refreshing to hear someone call out the gloveless keyboard warriors.

@edwardgrand

Жыл бұрын

I've got a latex sponsor ;) LOL Too bad I only need the gloves :(

@HughJanus9999

4 ай бұрын

Just getting into home mycology and it is incredible that soneone would EVER want to put gloveless hands on anything that is supposed to be sterile or sanitized!!

I just found your channel a few days ago. Idk how i haven't found it before but im enjoying all the knowledge you drop.

I subscribed also. Thank you for taking the time to educate people.

Man ever since I saw you on myco geeky podcast I binged your videos and must say that your awesome personality and humor is amazing and appreciate your knowledge. Mush luv brother

I love this channel

That was flawless, I'm really looking forward to starting real soon.

Great stuff as always....thanks a ton Ed. No FFU, but I started using your suggestions with my oven tech and things have worked great ever since. The little things do make a difference! For those bothered by the condensation, a hot mug of water on top of a stack of cooling plates goes a long way in drying them out. Mush appreciation Ed and thanks for all you give to this community.

It’s awesome you share stuff for free that you had to pay to learn. I feel like I’m watching someone with good practices. And I feel confident I will learn how to do it the right way the first time here instead of having to relearn. Stuffs hard enough for my dumbass to learn the first time

Great tips, Ed!

Michigan in the house I have to turn the music off cuz I keep singing along lol great video i always appreciate your enthusiasm n time Thanks

@edwardgrand

Жыл бұрын

So many Michigan people in Mycology. What happened to us? Alexander Smith, Hesler. Great mycologists! Now we're carrying on the tradition in a slightly different way :)

Thanks for the content!

🎉thank you Sir

Thanks dude just got my hood getting ready to do agar thank for the education

Just found this channel. I like this guy

@goatrock123

3 ай бұрын

The best!

Just made the mistake, despite knowing better, of pouring w/out gloves for the 1st time. I did not realize I had run out of gloves so decided to chance it with just alcohol. A dozen wasted plates, media, and several hours wasted, $???.00. The confidence that I will NEVER pour plates again w/out gloves...priceless. Thanks Doc!

@edwardgrand

5 ай бұрын

Sorry to hear that. I don't understand the no glove faction. Some kind of machismo, ego thing?

@toddwmac

5 ай бұрын

@@edwardgrand Thanks but I knew better, but now I really, really no better. :) Great marathon stream the other day. 9 hrs., wow! Good thing for CoPilot's summary feature. It is great when you and D. Keys get together. In appreciation.

@ZCRAIZED1

5 ай бұрын

I absolutely believe in wearing gloves. I can't argue the use at all, definitely a good things to do. I can't stand gloves on my hands and never use them. I'm not trying to promote anything at all. I simply can't tolerate them for more than a minute or two. I frequently pour 100 plates a week and have done thousands in my life. No flow hood, just an oven with the rack sitting on top and the door of the oven open with the oven on so hot air is moving up. A lot of alcohol frequently. 2 to 5 out of the 100 batch get contaminated. Frequently only 1 to 2 but occasionally 3 to 5. Good work in this video.

@edwardgrand

5 ай бұрын

I like to stack the deck in my favor.@@ZCRAIZED1

@MacCelium

5 ай бұрын

@@ZCRAIZED1I've had similar good luck with oven tek (hundreds not thousands for me) albeit with gloves. It is a simple approach more people could use, If I can ask, how do you store your poured plates? With no FFU, no way I can get a sterile bag over them so I roll the dice with the original bag with pretty good luck. Also, any thoughts on vented vs. non-vented plates? I'm seeing edge lately and was wondering if vents are contributing to it.

Thank you man

Brilliant

Watching you pour plates is effortlessly enjoyable. How many plates do you think you've poured by now Ed? 🤔

@edwardgrand

Жыл бұрын

OMG. I don't even want to do that calculation. It is easily in the 10,000s, maybe 100,000s. I used to use 6 cm plates in grad school and I stored them on sheet baking trays and rollers like they use in bakeries. AHHHH. PTSD!

How long do you let them cool down stacked in front of the hood?

I've learned a lot from your videos. I haven't grown anything yet. I'm still in the info gathering and tooling up stage. What's your opinion on glass vs plastic plates? I don't have a flow hood. Can I PC glass and reuse it?

@edwardgrand

Жыл бұрын

Personal, time and financial choices to consider. Different for everybody.

Thank you for this amazing video. I am just learning but is there a reason for not using media bottles with the no drip lip on them or is this just a personal choice to use the flask ? Also what is a vertical flow hood ? Mine sits a t the back of my work area and blows air over and towards me is that the same thing ?

@edwardgrand

Жыл бұрын

No particular reason for using a flask. Sometimes, I use a media bottle or even a liquor bottle. Whatever you use, make sure it fits in your pressure cooker (PC). Vertical and horizontal hoods/FFU are the same idea. Doesn't matter which you use. Thanks for the watching :)

Does the color help to make growth on the plates more visible or do you use it for record keeping? BTW, love to start my day with one of your videos!!!

@edwardgrand

Жыл бұрын

Mostly I just do it for record keeping. And I like pretty colors. If one plate in that color batch is bad, I can check the others quickly that are the same color. I found out today yellow isn't the greatest though...not enough contrast.

@edwardgrand

Жыл бұрын

Thank you for the lovely comment about starting your day. That makes my day!

Would u reccommend wearing gloves when handling plates? I always think about the finger prints and grime that builds up on the dishes. I just douse everything in iso now before opening😅

So I was wondering, when you do these methods do you leave the lid slightly open with parafilm wrapped around or do you just screw the caps on tight? Also which method out of the bunch is your preferred method?

@edwardgrand

Жыл бұрын

I like aluminum foil 'lids'. Easy to get a 'seal' that still allows air in and out to equalize the pressure.

Do you keep supplies and stuff for mycology in the lab or take your stuf back and forth? Just courious! I wish I had a lab or at least a ffu

@edwardgrand

5 ай бұрын

I have redundancies everywhere.

@Houseofmycology

5 ай бұрын

@@edwardgrand ahh ok

My agar plates would look like I'd just thrown it down some stairs😂perfect practice makes perfect.

@edwardgrand

Жыл бұрын

The fungi don't really care what they look like :) Lucky for me too! I'm not the prettiest specimen.

If you are pouring plates beside a bunsen burner (due to having no fume hood), would you wear gloves?

@edwardgrand

9 ай бұрын

Yes. Skin flakes and other detritus are all over your body. I try to always wear gloves when handling plates. It is a simple/cheap way to prevent extra vectors of contamination.

What are your thoughts on inspecting plates without gloves?

@edwardgrand

Жыл бұрын

Up to you ;) some people don't wash their hands after 💩 :)

🤘

Let’s grow 🤘😬

In the hood today😂

My mycelium actually eats the blue food coloring, so I guess they like it

@edwardgrand

Жыл бұрын

Lots of cool dye degrading enzymes in fungi.

What’s the recipe for your agar?

@edwardgrand

6 ай бұрын

GRYA recipe 2% Glutinous Rice/Whole wheat flour 2% Agar 0.1% Baker's or Nutritional Yeast Food Coloring (if desired) FOR 500 ml Total volume 10 g Glutinous Rice/Whole wheat flour 10 g Agar 0.5 g Baker's Yeast Food Coloring or 1g charcoal if desired. 500 ml tap water - Should make between 20-25 standard size plates.

@Houseofmycology

6 ай бұрын

@@edwardgrand the rice flour that’s just ground up flour? I’m definitely trying this? The rice says glutinous rice on the package?

@Houseofmycology

6 ай бұрын

@@edwardgrand what’s up edward I couldn’t find where it says glutinous rice at the store but can I use brown rice flour? Whole wheat flour is whole wheat rice?

@Houseofmycology

6 ай бұрын

@@edwardgrand does it hurt to add light corn syrup to the mix?

@edwardgrand

6 ай бұрын

@@Houseofmycology I wouldn't.

You’re blocking all of the back vents in the hood with the plates…

@edwardgrand

7 күн бұрын

The sterile air is coming from above. No worries. Non-pathogenic fungi. Limited work area.

I see your bottle says ethanol and not iso.. what gives cheaper, better, sterile and sip, what’s the reason for ethanol over iso.? Thank you very much. ( just kidding bout the sterile and sip, people please don’t dip into your supply (( seriously don’t get high on your own supply, you could die)). GO BLUE!!!

@edwardgrand

Жыл бұрын

Denatured ethanol is cheaper here that isopropanol. It's not the kind of ethanol you want to add to a drink...trust me...me and a friend tried. Works the same as iso. Slightly more volatile and flammable.

@jerodyoumans6002

Жыл бұрын

@@edwardgrand how much you paying per gallon of ethanol?

@edwardgrand

Жыл бұрын

@@jerodyoumans6002 $4

@jerodyoumans6002

Жыл бұрын

@@edwardgrand that’s pretty good. Right now I can get it for $1.00 until the current supply runs out.

@edwardgrand

Жыл бұрын

@@jerodyoumans6002 You should start selling that as automobile fuel!

Who is arguing against wearing gloves while pouring agar lol? Sometimes I wonder if you get singled out By trolls and government anti psychedelic bots

@edwardgrand

3 ай бұрын

Morons...you'd be surprised how much some people hate gloves...mostly dirty hippies. LOL

@etownKeystoned

3 ай бұрын

@@edwardgrand fuckin hippies, they are in no short supply in this space.. i just wish they would use deodorant and the chicks would shave... I think i would give them a pass on the gloves if we could just get those 2 boxes checked

So roughly 7 mins in after repeating him self about 50 times, he actually starts pouring plates…..f.*.*k me

@edwardgrand

10 ай бұрын

Do you feel better now?

@MacCelium

7 ай бұрын

@@edwardgrandDoubtful

@ZCRAIZED1

5 ай бұрын

Too funny! I do the exact same thing all the time. If you don't like what you are watching, change the channel! But either way, shut up!