Thank you for going through the trouble of acting out the procedures just to teach the rest of us. Much appreciated.

Dr. Grand, keepin' it real, as always.

6:10 Man, I'm so forgetful. I don't even remember the last time I forgot something.

@SuperiorDave

4 ай бұрын

Lol

@CrabbyPattydelight

2 ай бұрын

Me too, It can be frustrating when it happens daily, and gets expensive ,but well pull through with some TLC

Omg the tape over label is genius. Been using grafting tape for plates.

@edwardgrand

3 ай бұрын

That is funny. Someone mentioned using Scotch tape to stop Sharpies from running with alcohol on spawn bags the other day. I thought I said the thing about tape in a video somewhere, but couldn't remember where. Thank you :)

Keep up the great work 👍 👏 💪

There he is...thanks for this little tip Ed!! i just started doing this with little glass 10 ml containers from the dollar tree. Now i can store lots of genetics in a small area👍

@edwardgrand

Жыл бұрын

I'm always on the lookout for new containers. I think I've finally settled on the 50ml centrifuge vials and the 2ml cryovials. They are autoclavable and have nice hard threads on the lids. The cryovials are kind of tiny though. Really depends how much keistering I plan on doing ;)

I solved my labeling problem by making labels before I do any mycology work. Therefore, I just attach it to whatever I’m using at the time after I’ve completed the task. The only issue now is remembering to also put the date on them too! Ha ha Best regards from Detroit 🙂

@JoeMushrumski

Жыл бұрын

I use a Dymo 450 with 3rd party labels. When making the "template" in the software, there's date insert that auto changes to the date of the day you open the app. It's great, clean and you can pump out the labels for the day you're working. There are also some hardcore 3rd party labels that are freeze and autoclave proof. If you go Dymo, DO NOT get the new 550. They went DRM with the labels.

@Steveshappylittletrees

Жыл бұрын

@@JoeMushrumski - Excellent. Thanks for the tip🙂

I bought some Sharpie Industrial markers last year and they've been so much better than the standard ones. They work even on slightly moist surfaces, work again after getting wet with a quick line on a sheet of paper to dry out, and while still run with alcohol exposure are much more resistant to it then the old type.

@vincentwu2848

9 ай бұрын

Whoa, being able to write on moist surfaces is huge! But how easy is it to erase with alcohol? I've noticed that it becomes difficult to wipe off anything after it's spent a couple hours in the PC -- somehow even dry erase markers seem to permeate into polypropylene after a stint of 250F heat.

Great video but I wish you would have talked more about actually PCing the vials.

@edwardgrand

21 күн бұрын

What do you want to know?

Ed blessing us with the knowledge. 🍄❤️ Thank you, Sir. For all you do. ✌️

Have a good night teacher and classmates ❤❤❤

Thanks from CT Ed!!! Appreciate the knowledge

Thanks Ed!

Thank you from Az

Thanks for the great information Ed!!

The abbreviations drive me nuts. Talk about forgetting what the hell it is/was. And misplaced the logbook. hhahaha

@edwardgrand

Жыл бұрын

I put it in my phone and send myself an email every few weeks with it all copied.

Thank you for sharing your knowledge! I've been worried about storing my cultures long term with slants I'm going to try this instead

Love the commentary and sense of humor. I love the ideas for cheap plastic wrap. Costco roll of plastic wrap= forever tape. Thanks, Ed!

Thank you! Especially enjoyed the inside humor (thinking you have something special extraordinary, thinking you’re goin to slow down…). Technical tips also spot on of course! Thoughts on dehydrating colonized grain and vacuum sealing it as another long term storage option?

Love your videos dude

Dear Ed, Where have you been my whole life? Another classic Baby Grand, Once you get the message hang up the damn telephone, right? I'm gonna be more deliberate with my labeling and resist the urge to swirl and shake. My animal instincts had already told me it needs to be put it away and just forget about it and resist the urge to agitate the mycelium at work and yet, I'm a effing savage. I'm gonna break these bad habits in your honor. Thanks again Ed, Goodnight🎃

My vote is amazing .. thank you brother. Best KZread channel ever!!

@edwardgrand

Жыл бұрын

Thank you very much. It warms my heart to know I'm helping people and you're enjoying the content.

Hi Edward, love this video, thanks for posting! Do you place water in the cryo vials as well??

@edwardgrand

19 күн бұрын

Yes. I usually use about 30 ml before PCing.

If you put a link to Amazon for all these storage vessels, I would use the link. ✌🏻

@edwardgrand

Жыл бұрын

The links change every day. It would be outdated immediately. Just search '50 ml centrifuge tubes'.

Hope your doing well edd mush love at yeah 🍄❤

How long do you pc these for ?

Great information! You can store these both at room temperature and refrigerated?

@edwardgrand

Жыл бұрын

I just keep them at RT. Saves space in the fridge. Some species apparently don't like the cold.

ive been getting older for years . if i threw away everything i can,t remember then i wouldn,t have much lol. i label but get confused bout the abbreviations . i have the green centrifuge tubes but i hate poring and im afraid of trying no poor with those things . i hate boil over , very frustrating .

@edwardgrand

Жыл бұрын

Just PC tap water and throw normal agar pieces in there. Don't move it, keep it in a dark place. I love the sealable plastic containers/Tupperware. They hibernate. Good for many years. People have pulled live cultures from 20 year old water vials!

Thanks for posting another super informative video! I haven't seen anybody else demonstrate water storage. Despite the increased simplicity and longevity, for some reason a lot of people seem to prefer traditional agar slants. You had me sold at...50,000+ samples stored at room temp :D Btw do you mind sharing what the liquid you have your scalpels immersed in is? Is this a good substitute for using a sterilizer/alcohol burner/heat based technique?

@edwardgrand

Жыл бұрын

70% ethanol.

@vincentwu2848

Жыл бұрын

@@edwardgrand Wow, in your experience just leaving the tools submerged in 70% ethanol is a good substitute for flame sterilizing? I always suspected that flame sterilizing is overkill for our application but have no proof; but my only evidence for the efficacy of ethanol is information published by the CDC.

@edwardgrand

Жыл бұрын

@@vincentwu2848 I don't know if 70% is enough. I also flame sterilize. I always flame sterilize between transfers. The 70% alcohol jar is to store them and cool them down. After working for hours in the hood, the metal handles get quite hot. Hot blade in 70% is gonna kill anything.

@vincentwu2848

9 ай бұрын

@@edwardgrand Ahh okay. Thanks! I'm definitely going to have to copy your technique as it looks way too sensible to pass up on. Btw, there is some literature about long term preservation of fungal culture on anhydrous silica gel -- I'm assuming the type of fungi we are dealing with can't be stored with this method? Just thought I'd run the idea by you, even though I'm definitely going to be copying your water storage techniques as well :D

@edwardgrand

9 ай бұрын

@@vincentwu2848Lyophilization/freeze-drying techniques have been used with some fungi, but I'm not sure how well they work with filamentous fungi. I think most of those techniques are based on spore/conidia producing fungi. I've never explored it due to the cost of the machine and survival rates. I think the water technique might be best of it's ease, expense, and speed. Can you find me on FB, IG or Discord and send me the reference? I would love to discuss it more, but here it is hard. I don't think it'll let you post links here?

Do you suggest also keeping plates at room temp for storage also ?

@edwardgrand

Жыл бұрын

Yes. I keep everything at RT. Well-wrapped, they will be good for 6 months at least.

​ @edwardgrand Is there a source for this that establishes the longevity being claimed?

@edwardgrand

Жыл бұрын

Yes. It is quite well-known. You can find it on the internet. Sorry, I have read it many times, but I'm not up for an internet search.

@edwardgrand

Жыл бұрын

You can start here: www.researchgate.net/publication/248729085_A_miniaturised_system_for_storage_of_fungal_cultures_in_water

How long do you pressure cook it for? I couldn’t understand what you said! Love your videos!

@edwardgrand

8 ай бұрын

1 hour. Let them cool overnight before opening the PC.

@Houseofmycology

8 ай бұрын

@@edwardgrand ok awesome then I’ll be able to keep them on the counter for years?

@edwardgrand

8 ай бұрын

@@Houseofmycologyyes. Try not to disturb them, especially the surface pellicle.

@Houseofmycology

8 ай бұрын

@@edwardgrand I’m new to mycology what’s the surface pellical? I’m learning from your videos!

@edwardgrand

8 ай бұрын

@@Houseofmycology The layer of mycelium that forms on the top of the liquid. Like a SCOBY for Kombucha. It will make the underlying liquid anaerobic, which is what you want to keep the mycelium in hibernation mode.

how long do you throw these in the pc for?

@edwardgrand

3 ай бұрын

1 hour at full pressure and temp

@undeadzomboy

3 ай бұрын

@@edwardgrand the water needs a full hour? wild

@edwardgrand

3 ай бұрын

@@undeadzomboy It doesn't 'need' it, but I'm kind of an overkill guy. I'd rather spend an hour now instead of pulling cultures in 5 years just to find out they are all contam'd. Makes more sense to me.

@undeadzomboy

3 ай бұрын

@@edwardgrand thats fair! thank you!

Do you sterilise the water with the tube before ?

@edwardgrand

Жыл бұрын

Yes. Tap water. 1 hr at 12 psi. Lids 1/4 turn loose. Cover the whole rack with foil. PC. Cool. Tighten lids until use.

@ericboinet7378

Жыл бұрын

@@edwardgrand Thank you

@Sage_Advice

Жыл бұрын

​@@edwardgrandfor the small tubes too?

@edwardgrand

Жыл бұрын

@Sage_Advice cryovials yes. Eppendorfs no. They need a screw lid.

What about PCing them.

@edwardgrand

21 күн бұрын

What do you want to know?

@Mushmammy

21 күн бұрын

@@edwardgrand how long? I’m sure it’s 15psi lol. Also do we just leave the lids unscrewed like with anything else? Put them above the water level? Can we just PC water and then fill them in an SAB or in front of a FH? I appreciate you very mush 🥰

@edwardgrand

21 күн бұрын

@@Mushmammy 1 hour, 15 psi, lids loose, out of the water in a rack, fill in front of FFU

@Mushmammy

20 күн бұрын

@@edwardgrand TY TY TY!!!!!

"EDDY, OH YEAH BROTHERRRR!" (In my macho man voice) ! "Long term storage is coming, AND YOU NEED TO TO LISTEN UP BROTHER! “Best there is; past, present and future! Ohhh yeahh!” "Sometimes your agar is like too hot to handle, and too cold to hold!” “We’re in space, and space is the place!” “Expect the unexpected in the (mycelium) kingdom of Madness!”

@edwardgrand

Жыл бұрын

OH YEAH BROTHER, I CAN DIG IT! That damn Slim Jim commercial with Randy is embedded in my head forever. I'll try to do the voice on the next video if I can remember :)

@edwardgrand

Жыл бұрын

I tried... instagram.com/reel/CuOVifsxrAq/?igshid=MzRlODBiNWFlZA==

Very much appreciate the video bro!