PCR types in Hindi |colony PCR, nested PCR, inverse PCR, real-time PCR, rt PCR

Pcr types in hindi - This lecture explains about types of Pcr. Different types of Pcr technique discussed in this video including the following -
Colony Pcr, nested Pcr, hot start Pcr, inverse Pcr, rt pvr, real-time Pcr, quantitative Pcr, qpcr, touchdown Pcr, inverse Pcr and many more types of Pcr.
Stay tuned to know more about polymerase chain reaction types.
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Thank you for watching the molecular biology lecture on Pcr types and examples.

Пікірлер: 189

  • @monikasingh6839
    @monikasingh68396 ай бұрын

    Beautifully explained

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 ай бұрын

    Thank you

  • @Amar45611
    @Amar456113 жыл бұрын

    You are the best Sir! I salute your efforts towards teaching us with such integrity❤

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @sanjayagarwal2339
    @sanjayagarwal23394 жыл бұрын

    Sir you are amazing ...thank you sir

  • @narottamsahu1435
    @narottamsahu14354 жыл бұрын

    Thank you sir very nice explain in hindi ,, 🙏🙏

  • @pragyasingh6445
    @pragyasingh64455 жыл бұрын

    Thanku very much sir for all videos

  • @shadabkarim216
    @shadabkarim2164 ай бұрын

    It was explained superbly❤❤❤

  • @thecerebrum.
    @thecerebrum.5 жыл бұрын

    You’re the best sir!!!!!!!! You should get prize in biology 🧬

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you so much for appreciating my efforts

  • @anjaligupta6180

    @anjaligupta6180

    4 жыл бұрын

    yes..he should be awarded

  • @nidakhan8314

    @nidakhan8314

    4 жыл бұрын

    Yes indeed itny difficult topics itny asan tareqy sy explain kiye ha

  • @madanmohanmishra2759

    @madanmohanmishra2759

    2 жыл бұрын

    He has csir net qualified

  • @drshinwarishinwari1253
    @drshinwarishinwari12534 жыл бұрын

    I like your Hindi you explained very well in Hindi 😍👍🏻

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you. Glad you liked my lectures

  • @bindjoieiiouieasingh7029
    @bindjoieiiouieasingh70295 жыл бұрын

    Thankyou so much sir

  • @deepikadev9361
    @deepikadev93616 жыл бұрын

    thanks sir hindi vedio bnaane ke liye

  • @manishakhewadia6133
    @manishakhewadia61335 жыл бұрын

    Awesome.....thnks....aapke videos bhut achhe hote h nd helpful hote h👏👏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

  • @urbashisharma3893
    @urbashisharma38935 жыл бұрын

    Sir I love you soo much ...mne bahut wait Liya ki kB aap hindi m daaloge...am soooooo happy ..thanks

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad I can make you happy to learn

  • @ekramul3338
    @ekramul33386 жыл бұрын

    Thank U sir........

  • @ananditajha6718
    @ananditajha67183 жыл бұрын

    Really superb... Mr Suman. God bless you 😇

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @radhaagarwal9407
    @radhaagarwal94073 жыл бұрын

    Hats off 🙏🙏🙏🙏 sir thanks for this video

  • @swatichauhan2531
    @swatichauhan25313 жыл бұрын

    Excellent job sir👍👍👍 You have good technical knowledge .You explained PCR in such simple way that I am finding easy to learn all this.Your hindi too is good.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank You're very much for appreciating my efforts

  • @rishabhthakur3711
    @rishabhthakur37116 жыл бұрын

    Gjb sir

  • @snehak7582
    @snehak75823 жыл бұрын

    I am so used to your english videos that its weird to watch you explaining in hindi!😂

  • @totalgaming5792

    @totalgaming5792

    3 жыл бұрын

    Yas

  • @sweta8135
    @sweta81356 жыл бұрын

    Thanku sir.. It's really useful.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    You're welcome

  • @shikhakumari6575
    @shikhakumari65756 жыл бұрын

    Sir, kindly make a tutorial on lymphocyte trafficking.. I really need to understand it..

  • @samgaming91632
    @samgaming91632 Жыл бұрын

    Best of the best

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you so much for appreciating my efforts

  • @ayushisamriyar4732
    @ayushisamriyar47323 жыл бұрын

    Very well explanation sir.. Clearly understood by anyone.. Your way of explanation is so clear nd smooth... Thnku so much sir...

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @ayushisamriyar4732

    @ayushisamriyar4732

    3 жыл бұрын

    @@shomusbiologyofficial sure sir..

  • @aayushipathak6666
    @aayushipathak66665 ай бұрын

    Thankyou so much sir 🙂

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 ай бұрын

    You're welcome

  • @letstravel8840
    @letstravel88406 жыл бұрын

    Plz presentation on blast and fasta

  • @meghavatsa8191
    @meghavatsa81913 жыл бұрын

    Very well explained 🙏🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @payelbasu1321
    @payelbasu13213 жыл бұрын

    Sir your teaching is just incomparable to anyone ....please can U explain ITS region Nd related topics

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

  • @masroorkhalil9655
    @masroorkhalil96555 жыл бұрын

    sir your hindhi is best ever

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you

  • @masroorkhalil9655

    @masroorkhalil9655

    5 жыл бұрын

    Shomu's Biology si pls make a video on how to write thesis in scientific writing pharaphases in hindhi pls pls pls sir

  • @vasundhararaina6849
    @vasundhararaina68495 жыл бұрын

    Moreover in inverse PCR the DNA molecule used initially is basically wat a whole genomic DNA or a small part of it. Coz of its whole genome then adding a restriction enzyme may produce several small segments. How will we then isolate the desired one to be used for inverse PCR.

  • @jyotisingh448
    @jyotisingh4483 жыл бұрын

    Sir as we designed the second set of primers for the target dna in nested pcr why can't we designes the specific primers of the target dna for the common pcr.....sir kindly answer i really wanted to know

  • @amanagrahari12
    @amanagrahari128 ай бұрын

    too good sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    8 ай бұрын

    Thank you

  • @aboyfromhills2559
    @aboyfromhills25596 жыл бұрын

    First to watch. Thanks for every videos sir. I am going through your videos all my semesters. ♥️

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Glad you liked my lectures

  • @avinashkumar-ow7pq
    @avinashkumar-ow7pq Жыл бұрын

    Thank you for explaining the things in Hindi

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    You're welcome

  • @xubivet3295
    @xubivet32954 жыл бұрын

    Sir itna easy bana diya❤️

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @sushreemonali36
    @sushreemonali362 жыл бұрын

    Salute to you 👍👍👍🙏🙏🙏🙏🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @sahilprabhakar4788
    @sahilprabhakar47886 жыл бұрын

    Sir plz give complete information about ICAR - ARS exam

  • @deepikamahto8039
    @deepikamahto80398 ай бұрын

    Nice explanation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    8 ай бұрын

    Thank you

  • @sayantansaha53
    @sayantansaha533 жыл бұрын

    Greatest ever explanation Sir.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @sayantansaha53

    @sayantansaha53

    3 жыл бұрын

    Welcome sir

  • @rishtanandinisingh4526
    @rishtanandinisingh452611 ай бұрын

    THANK YOU SO MUCH SIR ! U R THE BEST BIOLOGY TEACHER ON KZread

  • @shomusbiologyofficial

    @shomusbiologyofficial

    11 ай бұрын

    Thank you so much for appreciating my efforts

  • @hemmahur1891
    @hemmahur18913 жыл бұрын

    Sir please make a video on Carrier detection in Haemophilia A PATIENTS by using linkage markers

  • @sandeshhulgunde5709
    @sandeshhulgunde57092 ай бұрын

    Nice hindi sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 ай бұрын

    Thank you

  • @user-vg6wm3fy1e
    @user-vg6wm3fy1e6 ай бұрын

    Excellent efforts...special thanks❤❤❤ i have one question, where does the primer attach the DNA strand in reverse PCR??? Because when known sequence is in mid, primer is attached at its sides and continues to add nucleotides further next to itself in the direction of known sequence but now the known sequence is at ends so how will primer be attached???

  • @pabitraamadhurima6960
    @pabitraamadhurima69603 жыл бұрын

    In the case of inverse PCR, if the sequence at the end are unknown then how do we identify the desired restriction enzyme to cleave and form sticky ends prior to the formation of circular DNA?

  • @SHUBHAMSINGH-gh6ln

    @SHUBHAMSINGH-gh6ln

    Жыл бұрын

    Same question

  • @gauriargulwar6035
    @gauriargulwar60354 жыл бұрын

    U speck very nice hindi,

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

  • @ImranAhmad-df3mi
    @ImranAhmad-df3mi4 жыл бұрын

    Thanks sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @tarannumkhatoon5070
    @tarannumkhatoon50706 жыл бұрын

    such me bahut hi achi vidio hai wo bhi hindi me

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    You're welcome

  • @Chia5555
    @Chia55556 жыл бұрын

    Thnku so much sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    You're welcome

  • @mukeshmurmu471
    @mukeshmurmu4714 жыл бұрын

    Thank you sir achha hai

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @nikitavatandar1666
    @nikitavatandar16663 жыл бұрын

    very nice vdo loved it

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you so much for appreciating my efforts

  • @arkhamyt6052
    @arkhamyt60522 жыл бұрын

    Sir in nested PCR , then what is the need of normal primers? we can directly add the nested primers on the target sequence and amplify our desired gene. Then what is the need of normal primers then?

  • @mamtarathore6641
    @mamtarathore66414 жыл бұрын

    Very very nice 👏👏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @rubabikram4619
    @rubabikram46194 жыл бұрын

    if no plz tell the difference between nested or hemi-nested pcr

  • @anshusharma2438
    @anshusharma24385 жыл бұрын

    thanks you sir👌👌👌👌

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @zulqarnanh
    @zulqarnanh6 жыл бұрын

    Awesome

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    You're welcome

  • @biologyc554
    @biologyc5546 жыл бұрын

    Nice video sir.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    You're welcome

  • @punambardhan9147
    @punambardhan91473 жыл бұрын

    I want to understand Degenerate PCR primer designing..Could you??

  • @shreyatiwari5271
    @shreyatiwari52716 жыл бұрын

    Awsm sir ...thanku so much jo ap hm logo k liye itna struggle krte hai ...thanku sir ..God bless u Sir mujhe apse ek Favour chahiye plzz help us ... Sir microscopy ke kuch video ap jaldi krne ka try kr skte hai ... I m sure apki list me to hoge hi ye topic but me thoda jaldi krne ka abhi favour mag rhi hu ...i hope u accept my request..thanku sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    I will make many more videos in hindi

  • @juwairiahmazhar9607
    @juwairiahmazhar96073 жыл бұрын

    👍👍👍

  • @komal9405
    @komal94054 жыл бұрын

    thankyou sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @sidrakhan8589
    @sidrakhan8589 Жыл бұрын

    Your Hindi/,Urdu video are much more good for understanding. Even though English are also v well elobarated. What about starting a separate new channel of shomu biology Hindi

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you

  • @yogeshanjansinger2872
    @yogeshanjansinger28724 жыл бұрын

    Thanku sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @rubabikram4619
    @rubabikram46194 жыл бұрын

    is hemi-nested or semi-nested pcr same?

  • @pinshusamariya7873
    @pinshusamariya78735 жыл бұрын

    Kya PCR hiv test me bhi kaam aata hai, or iski accuracy kitni he

  • @anilkohali6198
    @anilkohali61985 жыл бұрын

    thk u sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @ART-fs2wd
    @ART-fs2wd4 жыл бұрын

    Wow Sir your hindi 👌👌👌

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @ASHWANI81576
    @ASHWANI815763 жыл бұрын

    Please suggest any book of molecular biology i m bsc mlt student

  • @minakshee7240
    @minakshee72406 жыл бұрын

    Nice sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Thank you.

  • @arjitakaushal8116
    @arjitakaushal81164 жыл бұрын

    Sir... Please make a video about how to help pcr for diagnosis disease

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Okay sure

  • @indranisahu5527
    @indranisahu55273 жыл бұрын

    Nice sir ji 🤗🤗 PDF mil sakta hai Kya

  • @pk.educationclasses7733
    @pk.educationclasses77336 жыл бұрын

    Nice video sir ekad uhi hindi m b video bana dijiyega

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Thank you. Glad you liked my lectures

  • @Vanshikajain2
    @Vanshikajain22 ай бұрын

    If in the question paper given RT PCR then it is real time or reverse transcriptase PCR ???

  • @rambhawan2643
    @rambhawan26435 жыл бұрын

    Evolution video in hindi please sir

  • @BTS_army_V30
    @BTS_army_V302 жыл бұрын

    Hello sir . semi-nested pcr pr b vedio bna de .iska concept clear nhi ho rha

  • @sunilkumarsahu2283
    @sunilkumarsahu22836 жыл бұрын

    Sambhu bro I love you

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Thanks

  • @prabhudasnair5636
    @prabhudasnair5636 Жыл бұрын

    In Rt pcr why rna primer is used instead of dna primer? While in other type of pcr dna primer is used Please reply

  • @gautambharali4492
    @gautambharali4492 Жыл бұрын

    Late PCR kya hai please video sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Okay

  • @davidsweeney111
    @davidsweeney1116 жыл бұрын

    Shomu could you make this video in English as well please

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    It's already there

  • @Prabhjotsingh-fs3vp
    @Prabhjotsingh-fs3vp4 жыл бұрын

    Thanku sir make a video in Hindi medium ..... I wish u will make a video Hindi in future

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Sure

  • @tarannumkhatoon5070
    @tarannumkhatoon50705 жыл бұрын

    👌👌👌☺

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you. Glad you liked my lectures

  • @ilmasiddiqui110
    @ilmasiddiqui1103 жыл бұрын

    make ur profile on instagram too sir ...so that more people get to know about you ...and get benefited by watching ur vdos..

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Sure

  • @jaidtechy2762
    @jaidtechy27624 жыл бұрын

    Sir notes mil saktey hai?

  • @sonamdhanava3098
    @sonamdhanava30986 жыл бұрын

    Will I eligible for UGC net exam after the M. Tech in cosmetics..?

  • @rajarora4592
    @rajarora45924 жыл бұрын

    RNA RT-PCR is confermetory test and target not detected means

  • @anmol786
    @anmol7865 жыл бұрын

    Sir aap BSC zoology honours ki whole video hindi me bnao either payed video ya free depend on your .....bt mghe need h kaise hoga pls help me sir..

  • @devarshkumar9955
    @devarshkumar99554 жыл бұрын

    what type of polymerase we use in real time PCR as in taq man probe it uses exonuclease activity

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Probe or dye

  • @sunilkumarsahu2283
    @sunilkumarsahu22836 жыл бұрын

    Texonomy k upper video upload karo please

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    I will.

  • @tanvisharma812
    @tanvisharma8123 жыл бұрын

    Sir i have a question related to nested pcr.. That we are adding both outer and nested primers in a definite concentration... It is true that outer primer will amplify the unwanted DNA and nested one will amplify the target DNA.. But the whole process is going on for 30 cycles lets say... And the unwanted DNA along with outer primer they too are in the reaction till the reaction ends for 30 cycles how come we are going to identify wheather the unwanted DNA is not amplified as the reaction has outer primer and DNA till the end.... I mean are we adding any enzymes that will. Specifically cut the target DNA from the rest unwanted DNA

  • @rishiivirgo

    @rishiivirgo

    9 ай бұрын

    by gel electrophoresis. best example of nested PCR is identification of Rotavirus

  • @Pearlblack6387
    @Pearlblack638720 күн бұрын

    i need notes of this video urgently. can anybody have?

  • @bhagyashreechatterjee959
    @bhagyashreechatterjee9593 жыл бұрын

    Aamr akhon Mone hoi.. amr jibone zoology r kono chapter e r kono somossa hbena .. karon shomus biology ache ✌️✌️✌️✌️

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    I am glad to hear that you're getting benefit from my lectures. Stay tuned

  • @vasundhararaina6849
    @vasundhararaina68495 жыл бұрын

    Nested always keeps me wondering ABT it's need. Wn one already has the inner primer set then y to use the external one. Anyway practically things are not so common wd the theoretical aspects.

  • @rekhasaini7728

    @rekhasaini7728

    4 жыл бұрын

    Same here.... I am still not able to understand nested PCR 😢

  • @nathadil6765
    @nathadil67653 жыл бұрын

    Sir, can you plz upload csir life science videos

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Many videos are uploaded. There are more than 2300 videos

  • @hiteshpegu2844
    @hiteshpegu28445 жыл бұрын

    Can we use same pcr for testing dna of different species i.e human ,plant ,bacteria

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Different chambers

  • @kolasrinivasarao1096
    @kolasrinivasarao10963 жыл бұрын

    PCR English plz

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Already there

  • @yashpathology
    @yashpathology4 жыл бұрын

    Flanking sequence means sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Means the sequence present as single stranded region of DNA

  • @glowupwithnature145
    @glowupwithnature145 Жыл бұрын

    Why we don't use just nested primer ? Why we use 1st primer if it binds unwanted sequnces?

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    One primer set can make more unwanted amplification. Two set can reduce unwanted amplification

  • @tarannumkhatoon5070
    @tarannumkhatoon50706 жыл бұрын

    sir ap pls hindi me bhi kuch vidio banyakariye pls ☺

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    I am making more videos in hindi

  • @kumaresangomathykumaresan7817
    @kumaresangomathykumaresan78176 жыл бұрын

    Can you make videos in english plz

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    You will get this video in English in my channel as well

  • @Dineshsharma-qp6os
    @Dineshsharma-qp6os4 жыл бұрын

    Sir hindi me hi video banaya kro

  • @ilatiwari2807
    @ilatiwari28072 жыл бұрын

    Sir ,types of PCR ,csir net me pucha jata h kya?

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Yes

  • @NehaRajput-ly7do
    @NehaRajput-ly7do3 жыл бұрын

    Ur hindi is not that bad sir...... 🙂 ur method of teaching best in all the ways

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @anuragsingh-hp4ko
    @anuragsingh-hp4ko6 жыл бұрын

    I think Ye topic eng m cover ho gya h?

  • @shomusbiologyofficial

    @shomusbiologyofficial

    6 жыл бұрын

    Ues