Basically passed all my exams from organic chem to molecular biology related subjects in uni by refreshing the contents in my notes through ur lectures. Keep it up sir! Well done!

We do watch your videos all the time and they are so much helpful for us in biotechnology sir. We do almost all the exams in the university by watching your videos. You are capable of teaching even the hardest parts, in simple ways. We like to watch videos which you yourself teach in the white board, very much. Thank you sir...bohoth bohoth shukriya!!

@shomusbiologyofficial

4 жыл бұрын

Thank you so much for appreciating my efforts

The Best and Complete PCR Explanation Video Ever...👏👏 There are 100s of Videos on PCR, But this the best...! Thank You...!🙏🙏

@shomusbiologyofficial

4 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

I wanted this a few times- thank you for breaking down the process of PCR. You don't waste time on unnecessary words or pauses like many "youtube teachers" do,

your videos are good for brushing up concepts rather than reading all the books again when time is short. thanks.

I am very blessed found your tutorial lecture on Molecular Biology in the you tube, you gave a simple way how to understand the application and knowledge about molecular biology, you are very kind to share your knowledge to everybody in the world.....hope you get present from the God....salam from Indonesia....

IT IS SO HELPFUL!!! your explanation is so much better than my tutor's. thank you so much

thanks for perfect videos you share they're both informative and productive. It's brilliant that we have such awesome lecturers like you in KZread who are trying their best to inform others

@shomusbiologyofficial

4 жыл бұрын

Thank you so much for appreciating my efforts

BEST PCR LECTURE EVER🙏thanks a lot sir

@shomusbiologyofficial

Жыл бұрын

You're welcome

a really complete and informative video. good job and thank you :)

Your all videos are really helpful. Thank you so much.

Nice slide and explained every step in authentic way...

God bless u sir . Ur doing a grt help to thousands of people with ur beautiful videos..high quality teaching ♥️

@shomusbiologyofficial

4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

Thanks alot for making this subject easier may God bless you

Thanks...you just made everything so clear!!

very very lucid explanation. Thank you sir.

Thank you sir for the good work .... I have understand the concept of PCR very easily through ur lecture, it's very helpful. . Please upload more videos related to this . Your explanation is very good ....

i am getting hope to clear exam only because of your videos. after 8 yrs gap, i started to persue my carrier, its such a long break , ur videos given me much confidence . i hats off u. u r my role model thanks shomu sir.

@newthinker5829

4 жыл бұрын

Hi r u working now... I am also started studying after 8 yrs of gap hoping to get a job soon

Thank you very much, very informative and helpful video

Thank you very much for your video lecture, much appreciated.

Great lecturer, thank you

Very interesting sir as you said .thank you very much for the lecture.you have provided us with very minute details which are Essential for attending the long questions ans . ❤️❤️

Thank you very much! This is really helpful!

very helpful . The content is very detailed. Great job sir.

@shomusbiologyofficial

5 жыл бұрын

Glad to hear that you are getting benefit from the videos

Very informative video. hoping for t same for other techniques....thanks slot it really helped me

Work well done. thanks sir

U ARE THE BEST.... HELPING ME ALOT DURING MY STUDIES. THANKS

@terrybash5194

2 жыл бұрын

Thank you so much DR RORPOPOR HERBAL on KZread you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes..........

thanks a lot...very nicely explained

It is so much helpful, very informative and descriptive video. Thank you sir😇

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

Thank you so much. It was very helpful.

Thankyou very much for such an informative video.. keep it up.

@shomusbiologyofficial

6 жыл бұрын

+Savita Ct thank you. Glad you liked my lectures

it was an awesome video. 😊 very helpful

Excellent video!

great job God bless you 🙌🙋

very good technique

very informative

thank you Dr Shomu

@shomusbiologyofficial

3 жыл бұрын

Glad to hear that you're getting benefit from my lectures

it is very helpful my lecturer recommend me to watch this

@shomusbiologyofficial

4 жыл бұрын

Glad it helped

excellent video

Tq so much for better understandingsir......I like ur teaching skills sir.....

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

i'm starting to have hope, Thank you sir

@shomusbiologyofficial

4 жыл бұрын

You're welcome

I didn't understand this process even though my teacher took 3 hrs to explain it.. Thanks sir for the explanation

hello tysm for this video.u r genius.u helped me in molecular biology n genetic engineering so much..can u plz provide me yr written material or books...???

great video. tnx

Wel Done sir !

This is very helpful, my doctor ask us to watch your video and it best decisions ever,

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

@terrybash5194

2 жыл бұрын

Thank you so much DR RORPOPOR HERBAL on KZread you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes..........

Very helpful thank you so much🙏🙏🙏

@shomusbiologyofficial

4 жыл бұрын

You're welcome

it sounds good thanks alot

IT IS VERY MUCH HELPFULL

very good.

very informative video

@shomusbiologyofficial

8 жыл бұрын

Thank you. Glad you like my lectures. Stay tuned.

Very nice...

hi shomu i have a question reagarding pcr reaction.. why initial pcr product produce long and varying size product in reaction, and fina reaction produced shorter and uniform size product?

আপনার video গুলো অনেক সুন্দর। অনেক concept clear হয়েছে। আপনাকে অনেক ধন্যবাদ।

@shomusbiologyofficial

4 жыл бұрын

Thank you for appreciating my efforts

Thank you so much sir 🥰🥰🥰🥰

@shomusbiologyofficial

Жыл бұрын

You're welcome

Hello sir, your videos are very helpful to all of us ( me and my batchmates )sir we all just watch your videos and we all have passed our exams with good Marks thank u soo much sir for making every topic so simple to understand. sir I am facing problem in plateau effect in PCR ,I am not able to understand it, so sir can you please help me out

@shomusbiologyofficial

4 жыл бұрын

Thank you. Glad you liked my lectures. Please subscribe and share

Thank you very much sir...oshonkhyo dhonnobad

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

very good keep it up

thank you

Thank you very much sir.

@shomusbiologyofficial

3 жыл бұрын

You're welcome

@terrybash5194

2 жыл бұрын

Thank you so much DR RORPOPOR HERBAL on KZread you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes..........

Really very helpful sir....plse sir can u explain suicide pcr in another tutorial video.

good lecture

@shomusbiologyofficial

5 жыл бұрын

You're welcome

sir, i am confusing the order of the videos, recombinate DNA Technology 61 tutorials are there , but which one is 1st , 2nd..........61 videos, this is my confusing , i hope you pls clearify , thank u.

A primer may bind anywhere on the DNA, obviously to ours complementary sequences but it's not always necessary that these sequences will be present at the very start. So the sequences where primer doens't bind how are those sequences amplified?

impressive command of English

Thank u very much sir. This really help me to understand more about PCR.

Thanks

Got one question! when a gene is encoded on a single strand of DNA called template strand then why do we perform PCR on both strands of DNA using 2 primers?

inspite of being such a good method of amplification why cloning is not completely replaced by pcr?

Thank You!!!

@shomusbiologyofficial

3 жыл бұрын

You're welcome

sir plz explain chemical. synthesis of dna & its 3 types

Thank you very much just a question about the oligonucléotides they are of DNA or RNA????

hello sir, help me to clear my doubt that initially two primers are used for 1st cycle but how many primers are used for 30 cycles

Helpful..

@shomusbiologyofficial

4 жыл бұрын

You're welcome

ty very much

What are the different enzymes used for this process

Why all videos on PCR r not available at u tube???plz make them easily access for all students those who can't afford coaching...

Sir I one big request.. can you make one video about enterococcus feacalis full detail

How sir the dna denaturate at specific site, if you provide heat it should denaturante completely?

Thank you bhai 😭

@shomusbiologyofficial

Жыл бұрын

You're welcome

nice

Does anyone have any experience with trying to PCR with loading dye present? Or any ideas on how to get rid of it so that it doesn't inhibit the enzyme?

what is means by melting temprature

In which base pair does the primer starts binding in annealing steps? It binds to complementary sequence but in which exact bp does it bind?

@craftsandscience860

8 жыл бұрын

It binds first at the 3' end of the primer. That's why the 5 base pairs on the 3' end of the primer are very important In order to promote a proper annealing to the DNA complementary strand. Therefore, better to design a primer with G or C nucleotide at the very end of the 3' end of the primer because it has a strong binging efficiency

Sir why primase is not used ?is that bcz of small size of DNA fragment that is to be copied

Why both primers ?

Heat labile means heat sensitive. So Taq polymerase is not heat sensitive and neither heat labile!!!! Thank you for your video.

@shomusbiologyofficial

2 жыл бұрын

You're welcome

I was wondering... What would happen if the two primers were on the same strands ?? Would there be any PCR's product or nothing ?

@waiting12

7 жыл бұрын

Mike Hunt thinking same ...if we suceeded to any how artificially ....dna manuplating by pahogens could be reduced...

Hi I have a very basic Question regarding PCR. PCR is used to amplify content of DNA. Say for ex. My sample contains 38ng/microL concentration of DNA, using PCR Am I making my solution more concentrated? (from 38 ng to 38 micro/mg?) Please clarify..

@csmobilesolutions8553

7 жыл бұрын

Health Support yes

Grt👍

@shomusbiologyofficial

3 жыл бұрын

Thank you

we need to amplify only one strand of the DNA,as,the sense strand contains the required fragment of DNA,then why do we need the anti-sense strand of DNA for amplification??? why do we add two primers in that case,,,,when we need to amplify only the sense DNA strand?

@SuperAnnanymous

9 жыл бұрын

Sagnik Biswas Because anti sense strand is complementary to the sense strand. We actually get sense strand by amplifying an anti sense strand. This is my thinking. I maybe wrong.

@sagnikbiswas7045

9 жыл бұрын

You're right ☺

what taq polymerase is important

@mdwazid1548

7 жыл бұрын

akriti nipu coz it can resist high degree of temperature

I am afraid that this video is 30 min. hope i will get it

Sir iss topic ki note kaise download karein

@shomusbiologyofficial

2 жыл бұрын

Don't have note now

👏👏

nice english accent!!!

اكو عرب اهنا 😂😂

PCR made easier thanks

Plz speak a little faster