Its a good try, but there are few things which has to be dealt for better understanding like: 1. what chemicals are used specifically to cleave a certain nucleotide (G-DMS, A-Formic acid, C-Hydrazine+NaCl, T-Hydrazine) together with piperdine to cleave the phosphodiester bond of modified nucleotide 2. How to make single stranded DNA fragments (heat denaturation or using helicase) 3. Dimer formation are in combinations of A+G and C+T If a band in the DNA sequence appears in both the G-reaction and the G+A-reaction lanes, then that the nucleotide is a G. If a band in the DNA sequence appears only in the G+A-reaction lane, then it is an A. The same decision process works for the C-reaction and the C+T-reaction lanes. 4. What quantity of sample DNA is required for sequencing (if not sufficient, have to run PCR or do cloning to amplify) 5. What about the left part of DNA strand after cleavage (they cant be visualized, due to lack of isotope) 6. What percentage of gel is preferred (6% polyacrylamide gels are used, bcz they are too sensitive in differentiating the DNA bands with 1bp size)

@ProfSardarMNiaz

7 жыл бұрын

Praveen Kumar you are stunning.help me a lot

@savitapal9943

6 жыл бұрын

hello friends

@rishabhjain7690

6 жыл бұрын

Praveen Kumar Thanks

@roshanaatreyapaudel4033

5 жыл бұрын

Thanks

@yingliweng9759

5 жыл бұрын

thanks

I just realised that I've been passing my entire uni thanks to you but never said a proper thank you so: Thank you! (=

@Sheikhandbirds

3 жыл бұрын

Same 😂😂😂

@rosh_serendipity939

Жыл бұрын

Samee 🥹

I'm doing an essay on genomic analysis, and these videos on sequencing are amazing for a proper, scientific understanding of how sequencing has developed over the years. Thank you so much for the clear and helpful video!

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

I came across this video , and may i say you are the best youtuber teacher we have got Hands down sir, you are brilliant

seriously... The Best Lecturer Out there.. I find it so effective.

Interesting and very clear explanation. don't bother about the time because it's very interesting and understandable. Thanks shomu!

when you write down on board that method is good for understanding and due to only this difference between you and other you tubers i was watching your videos

no need for short videos....you are doing great job... because it is helpul for begginers,,,if you start to make short videos ...then the beginners will not understand....thanks alot...very helpul.....

@shomusbiologyofficial

7 жыл бұрын

+Ejaz Khan thank you.

I am a veterinary student .....GADVASU....number one veterinary university in india......still we dont have any teacher like u sir......thank u very much❤️❣️

@shomusbiologyofficial

4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

Dude you are the BEST! KEEP IT UP

Thankyou sir.....you re the best teacher...😍

indeed its helpful...........complicated topic bt after watching twice /thrice i can understand for sure..

@shomusbiologyofficial

7 жыл бұрын

+Alwina Anam thank you very much

This is so helpful but I almost got confused at the initial result interpretation but thank God, I later understood it. Thank you Shomu Biology

@shomusbiologyofficial

11 ай бұрын

You're welcome

I love your explanation♥️ Thank you so much ♥️

Awesome. It helped me a looooot. THANK YOU

Beautiful sir... God bless you.. 🙏🙏🙏🙏🙏🙏🙏

thank's very much now I depend on you very much to understand any thing .I wish I Can reply the favour ❤❤ and please don't stop many students depend on you . Marwa from egypt

but what about the first nucleotide ? how to know it?

Really really helpful... Understood the topic thoroughly... ☺️

@shomusbiologyofficial

7 жыл бұрын

+mayur tupe thank you. Glad you liked my lectures

@CringeDealer69

7 жыл бұрын

Shomu's Biology My pleasure... ☺️

My best BIOLOGY teacher over whole youtube

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts

@drsahivlogs676

3 жыл бұрын

@@shomusbiologyofficial Love From Pakistan

1. Not Chemical Synthesis. It is Chemical Cleavage (two completely opposite meanings) 2. First nucleotide determination without which DNA sequencing is incomplete. From my understanding, you are supposed to initially cut/cleave the DNA sample with a restriction enzyme. From this you get to know where the DNA is getting cleaved (as you know the restriction sequence of the enzyme). This is then followed by the steps explained in the video.

@sukhmanpreetkaur7951

4 жыл бұрын

can u plz refer me any book for this topic

Awesomely explained. This is my first video on Maxam-Gilbert, n you cleared the concept seamlessly. I won't go into technical details, I will just say that concept-wise this lecture is awesome. :-}

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@anjiman5890

3 жыл бұрын

@@shomusbiologyofficial,your class is really helpful, sir you take an example of 12 nucleotide, and in autoradiography in the 11th portion the 12th nucleotide come..can you please explain it

Sir your channel is v helpful . I get everything here

Thank you very much Sir. It helped me a lot

Thanks for this video. It's really helpful for me.....This topic is in my syllabus....thanks a lot

@shomusbiologyofficial

2 жыл бұрын

You're welcome

thank you... great explanation...

doing a great job man (Y)

you have a simple way in teaching and presenting the idea

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

great explanation. thanks :)

The video was quite helpful and made understanding easier. Thank you very much

@shomusbiologyofficial

5 жыл бұрын

You're welcome

simply awesome!! got it :)

praveen Kumar.....thanks for adding more information

Thank you sir for explaining it beautifully!

@shomusbiologyofficial

5 жыл бұрын

You're welcome

sir carry on your vedios are very helpful

It’s very clear nd helpful .thank you for this🙏🏻

@shomusbiologyofficial

2 жыл бұрын

You're welcome

Very nice sir ! no words to tell and your classes helped a lot. thank you soo much sir

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

You are the best💜💜💜

thanks for your explain

infact it is helpful. Great.

Great work

Better than my class work ❤🎉

@shomusbiologyofficial

3 ай бұрын

You're welcome

@Serwada237

2 ай бұрын

Iam so happy that you replied to me I have used your site to undertand concepts for my molecular biology masters degree. At Makerere university Uganda… thank you we appreciate your efforts to teach the world science

waah sir...lovely...very easy

I think whole biotechnology students are graduating seeing your videos ❤

@shomusbiologyofficial

2 жыл бұрын

Thank you so much for appreciating my efforts

@mahiratariq7864

Жыл бұрын

Yeah🥰

@Akshita23436

Жыл бұрын

Absolutely right

that was some top explanation man. thamks!

@shomusbiologyofficial

Жыл бұрын

You're welcome

Thank you so much SIR

you are AMAZING

How did you know that the very first base in the gel is Adenine?? Someone pls answer

Thank you so much! You helped me alot. I owe you.

@shomusbiologyofficial

5 жыл бұрын

Thank you. Glad you liked my lectures

@khalidmehsud54

3 жыл бұрын

Hain?

Thanks! what is the sequencing QiaSeq target amplicon method different from Sanger sequencing method ? which is superior?

I love this guy

i have a question.. why we select G and C bases separately.. i mean we took purines and pyrimidines in two test tubes n G and C in rest of the two so why not A and T??? and we treated T+C tube with the chemical to cleave T and upto some extent C as well.. so why that chemical isn't working for T?? n why the result is same for C and T+C???? reply

Great job sir

Thank you sir

Thanks alot👌👍

Thank you sir....

Thank u very much

And what about first base Adenine...gel doesn't have this info.. so whats the reason sir..plzz...tell us

It's really helpful...

@shomusbiologyofficial

6 жыл бұрын

Thank you. Glad you liked my lectures

Sorry sir can you tell me a little beat in interpretation of result sequence if you have C and G of the same size what base will you labble first

Awsome great work love you

Thankyou for the wonderful explanation sir❤❤

@shomusbiologyofficial

8 ай бұрын

You're welcome

It's really helpful.. Thank you so much

@shomusbiologyofficial

2 жыл бұрын

You're welcome

plzzz sir explain this in black board . its a humble request from my side because i saw this explanation in black board on many other u tube channel's. but for me sir only u have abality to explaining in black board with ease. otherwise this presentation is also very good sir👍👍

Does each reaction require many single-strand DNAs ? ( cause there is too many fragments )

Thanks a lot.

thank you bro i got help from this video.. keep it up

@shomusbiologyofficial

6 жыл бұрын

Thank you

Thank you very much sir... 🙏

@shomusbiologyofficial

2 жыл бұрын

You're welcome

thank u so much!!!!!!!

Great sir very easy and understanding english best method of teaching 🇵🇰

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

THANKS U SIR TO INFORMATIVE

good lecture

U are a super teacher 😍🙏

@shomusbiologyofficial

4 жыл бұрын

Thank you.

Good job

Bamh1 recognise 6 base pair of DNA in palindromic sequence if the first part sequence is given below what is rest of it? 5 C A A3

Won't their be be combinations for cleaving out T in 3rd tube Like 1 and 2 T And another one 1 and 3 , 1 and 4, 2and 3, 2 and 4?

Sir I have a question As we can see that while chemical degradation sequence is cleaved at the site just before to that perticular residue so what about A which is radiolabled as a first residue. How we will know that A is present at first becs when it get cleaved we will get only phosphate without sequence?

Thank you soo much professor 🙂

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

How can you identify A at Terminal 5 prime end. As it shows the band in T+C.

extremely helpful!!!

@shomusbiologyofficial

7 жыл бұрын

+samjhana stha thank you. Glad you liked my lectures

Thank u so much.. really helpful

@shomusbiologyofficial

7 жыл бұрын

+Suprita Dash Glad it helped

Thank you so much sir. Your teaching method is very easy and very helpful for understanding. Sir please make a video lecture on Evolutionary Game Theory.

@shomusbiologyofficial

3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@tusharsujgure7379

3 жыл бұрын

@@shomusbiologyofficial sir please make video on Evolutionary Game Theory

Great...... Thank you

@shomusbiologyofficial

3 ай бұрын

You're welcome

During chemical termination is the single strand added newly to each test tube everytime?

Maxan and Gilbert sequencing explain very easily.. Suman sir please make video lecture on Game Theory..

@shomusbiologyofficial

3 жыл бұрын

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@shubhamphatangare6062

3 жыл бұрын

@@shomusbiologyofficial sir please make video lecture on Game Theory

but how can we get the first base by using this method

Thx ⭐️

Like many others I also want to know whether 1st base can be detected correctly from this method or not.......if not why

Cysteine.....😅 repeated twice lol But nice explanation as always your videos are helpful keep it up👍

thanx a lot

Sir why we Have To elute out the heavier band and work with only the lighter band after the denaturation of DNA double-strand?? Please reply sir

At 1 guanine reaction ,u told that DMS will cleave the guanine ,so no stretch of DNA will form . But how it produce 3 types of strand at guanine reaction ,because DMS will break sequence ,so we will get same type stretch DNA only sir ,???

amazing video

@shomusbiologyofficial

4 ай бұрын

Thank you

why the first base (A) is not identified..?

Sorry sir...i m not able to getting this in last...how we do count ...if we don't know the sequence of complementary then how we count the Nucleotide bases....i saw this video repeatedly but i didn't get it after electrophoresis ..... please tell

Can u give the link of the biochemistry book

just good

In the results interpretation part, you appear to be removing even terminal nucleotides (like you removed the C from the T+C tube). Didn't you say that the terminal nucleotides cannot be removed ? Also in what manner are the nucleotides being fragmented ? There should be atleast 4 different types of fragments in the G tube for example if we remove the G from the middle.

The last step is quite complicated. ..

Thank you

@shomusbiologyofficial

6 жыл бұрын

You're welcome

Thank you 😊

@shomusbiologyofficial

3 жыл бұрын

You're welcome