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Inside a CRISPR Lab

At UC Berkeley, CRISPR researchers are developing better gene-editing enzymes and more efficient delivery into tissues. These innovations will be used to improve the effectiveness and lower the cost of life-saving therapy for genetic disease. Take a peek behind-the-scenes to see what goes into a human genome editing experiment.
Produced by Marica Petrey and Leah Worthington
Cinematography by Mogli Maureal
Edited by Marica Petrey
Sound and color by Mogli Maureal
Featuring Lorena de Oñate, Ph.D. and Rima Mendonsa, Ph.D.
Special thanks to Megan Hochstrasser, Ph.D., Innovative Genomics Institute, UC Berkeley
Music courtesy of Audioblocks.com

Пікірлер: 57

  • @Aztorak_1
    @Aztorak_1Ай бұрын

    Now to unveil my superior alternative: CRUNCHR. In all seriousness though this is excellent.

  • @jibraankadri
    @jibraankadri4 ай бұрын

    very well filmed and documented. great job.

  • @huntingkc1
    @huntingkc12 жыл бұрын

    So whomever did the recording and editing did a great job

  • @leo_amorim
    @leo_amorim12 күн бұрын

    Excelent job on this video. I was studying crispr theoretical part and this video helped me to understand it pretty well. Thanks!!

  • @zqxzqxzqx1
    @zqxzqxzqx12 ай бұрын

    I've been fascinated by CRISPR Cas-9 since it's inception. I follow all the news I can get, and have read Code Breaker (which was more interesting and informative than I expected!) I'm excited to see it already restoring sight and correcting sickle cell. Thanks for this look into a lab.

  • @peters7278
    @peters72783 жыл бұрын

    Thank you for the video! On a side note, I think this process would be an great candidate for automation. It seems that the human has to complete soooo many steps...

  • @LarsLarsen77

    @LarsLarsen77

    Жыл бұрын

    It is automated, they're "teaching" people to do it for $100,000 a year, which those people somehow think benefits them, but really they're just doing what a robot already does.

  • @sandeepgolui8129
    @sandeepgolui81293 жыл бұрын

    Excellent to see...thanks for sharing madam

  • @farahbourek2120
    @farahbourek21202 жыл бұрын

    Can you please give me the details about methods of extraction that you use to get crispr cas 9

  • @Tina-bo1sf
    @Tina-bo1sf3 ай бұрын

    I thought I saw a comment saying this but couldn’t find it. Could you PLEASE figure out the code sequence for the vaccine, then reverse it and fix us??? I’m in California. I’ll be knocking on your door soon. I was 49 when I took it. I’m 51 now. I had heart pain, dizziness, almost passing out and no more periods. Please fix us.

  • @nathanielyoundt4936

    @nathanielyoundt4936

    3 ай бұрын

    The sequence for the mRNA vaccine is in the public domain. No need to decrypt it 👍🏼.

  • @farahbourek2120
    @farahbourek21202 жыл бұрын

    Please can you give me the methods of extraction that you use to get crispr cas 9. Thanks

  • @justanotherguyful
    @justanotherguyful2 жыл бұрын

    It doesnt actually explain the entire process. A lot of the stuff theyre doing looks like it can be done in anyones garage. Very simple process its just that no one has a video actually showing the real lab work from start to finish. If someone made a 20 minute video actually detailing everything from start to finish then anyone could be a genetic engineer if they purchased the right tools.🤓

  • @volution1160

    @volution1160

    Жыл бұрын

    anyone can. purchase the books, learn online, buy the tools, do the experiement, coalese findings in scientific paper, and if it gets published Id say noone would argue you are not a scientist. Prestige, social hierachy, elitsm etc. are why you would probably run into more problems than someone employed at an institution doing exactly what you do in your garage.

  • @kazekagekid

    @kazekagekid

    Жыл бұрын

    @@volution1160tbh I’m more interested in knowing how they’re so certain they won’t introduce their target sequence backwards to the cas9. Do they always have to find the RNA from elsewhere or are we flying actual base pairs together at scale with some sort of machine? the sheer number of molecules in every drop, and the random motion of those molecules within the swirling medium feels like way too much uncertainty.

  • @LarsLarsen77

    @LarsLarsen77

    Жыл бұрын

    Newsflash: You can do crispr in your garage. Nobody's even checking if you know what you're doing or not. (unless you're in europe, then it's super illegal)

  • @LarsLarsen77

    @LarsLarsen77

    Жыл бұрын

    @@kazekagekid It doesn't do it backwards because the guide RNA only binds to the sequence in one direction. You design it to match the target sequence and no others, but sometimes it will make a mistake if you designed it poorly.

  • @LarsLarsen77

    @LarsLarsen77

    Жыл бұрын

    @@kazekagekid And yes you have to give it a target sequence (already in the right order) to stitch in, or else it will just stick whatever is around in there, which will not be what you wanted.

  • @shin-ishikiri-no
    @shin-ishikiri-no4 ай бұрын

    I get that this can be used for life-saving gene modification, but it can be much more than that.

  • @bharat1031
    @bharat10313 жыл бұрын

    cool lab

  • @danlaw1208
    @danlaw12083 жыл бұрын

    Cool job ever

  • @eukaryotic0703
    @eukaryotic0703 Жыл бұрын

    How is the guide RNA made? does it have any special feature or is it just extracted mRNA?

  • @mockondo3011
    @mockondo30116 ай бұрын

    Does this lab sell the purified Cas9 protein? Can it remain active through several days of shipping?

  • @joyaborthakur9407
    @joyaborthakur94072 жыл бұрын

    Please Scientist 🙏 Try to reverse the biological aging process in humans.. And also to eliminate age related diseases..

  • @AsuraVeri

    @AsuraVeri

    Жыл бұрын

    Why? I see no good coming out of it.

  • @FahimHoq

    @FahimHoq

    Ай бұрын

    ​@@AsuraVeriwhy not

  • @mariadelcarmenmendoza8721
    @mariadelcarmenmendoza87213 жыл бұрын

    Great video!!! She's so sweeet :3

  • @mrnerd73
    @mrnerd73 Жыл бұрын

    Thank you for the video ❤️ Learned a lot... Liked your way of presentation

  • @ghulamrasool206
    @ghulamrasool206 Жыл бұрын

    Fascinating

  • @kashifkashif427
    @kashifkashif4272 жыл бұрын

    I have never seen such lab

  • @asnakegetachew
    @asnakegetachew3 жыл бұрын

    Great!

  • @kavithaalapati9850
    @kavithaalapati98502 жыл бұрын

    Useful and informative video

  • @user-bv5lz7fw5m
    @user-bv5lz7fw5m Жыл бұрын

    저도 여기서 연구하고 싶어요

  • @ferryjap5515
    @ferryjap5515 Жыл бұрын

    Can i order CRISPR and deliver to out of your country?

  • @griggsentertainment6463
    @griggsentertainment64632 жыл бұрын

    What city is the Lab in?

  • @JohnDoe-ml1ui
    @JohnDoe-ml1ui3 жыл бұрын

    Don't judge me.... i don't know what she is doing but that "tube scraping" at 3:32 doesn't look professional made at all 🤣

  • @MrLovemydog12

    @MrLovemydog12

    2 жыл бұрын

    She is trying to break up the pellet at the bottom of the tube. These cells want to stick together, therefore the small vibrations help break up the clusters of cells. Flick mixing such a viscous solution of cells will not properly mix, but pipette mixing (and vortexing) could damage the cells. Eukaryotic cells are much larger than bacteria and must be treated with care, using the grate to vibrate the cells is actually pretty genius if you ask me.

  • @JohnDoe-ml1ui

    @JohnDoe-ml1ui

    2 жыл бұрын

    @@MrLovemydog12 Yeah i know but... I mean... still don't look professional 😂 they should make a machine or something made on purpose to do that if it's something needed...

  • @AdrianDucao

    @AdrianDucao

    2 жыл бұрын

    @@JohnDoe-ml1ui lol you mean you need a machine that just flicks a tube couple of times? What a waste of time and energy...

  • @franklinlingga5491

    @franklinlingga5491

    2 жыл бұрын

    @@JohnDoe-ml1ui what machine for that simple work? wasted

  • @saltandpepper3892
    @saltandpepper38922 жыл бұрын

    They can make zombie I guess

  • @sandeepgolui8129
    @sandeepgolui81293 жыл бұрын

    🇮🇳👍🙏🙏👍🇮🇳🧬🧬🧬🧬🌐🌐🇮🇳

  • @tx-sweet-pjg3547
    @tx-sweet-pjg35473 жыл бұрын

    You know Frankenstein was a monster right ?

  • @royaniv

    @royaniv

    3 жыл бұрын

    huh?

  • @FahimHoq

    @FahimHoq

    Ай бұрын

    That's fiction

  • @patricktibbits5475

    @patricktibbits5475

    26 күн бұрын

    Dr. Frankenstein was the creator of a monster.