Extracting Plasmid DNA: How To Do a Miniprep

In this method video, Molly takes us into the lab to teach us how to purify plasmid DNA from a liquid culture of bacterial cells. When purifying plasmid DNA from a small volume (3 to 5 ml) of bacterial cells as we do in this video, this technique is called a miniprep.
View this video (and more like it) on LabXchange: www.labxchange.org/

Пікірлер: 65

  • @user-zd7xv7wc2t
    @user-zd7xv7wc2t6 ай бұрын

    This video is one of the best teaching for learning extraction plasmid.

  • @samsonkamau5048
    @samsonkamau50489 ай бұрын

    I am not used to commenting but this one deserves a thumb's up. excellent explanation with clear and precise illustrations

  • @birdo54
    @birdo54 Жыл бұрын

    This is a FANTASTIC video! Thank you very much, this was extremely well done.

  • @LabXchange

    @LabXchange

    Жыл бұрын

    Glad you enjoyed it!

  • @bullymag
    @bullymag Жыл бұрын

    This work deserves way more than that amount of view. Excellent work. Liked and subscribed!

  • @shirinbehnamsani2366
    @shirinbehnamsani2366 Жыл бұрын

    The first time I understood what I was doing this whole time in the lab. Thanks for the amazing explanation!

  • @mateuspaiva5916
    @mateuspaiva5916 Жыл бұрын

    The material in the video is amazing, and the scientist is also an amazing actress! Great job in here, keep up with your inspiring work.

  • @mobinahsansiddiqui
    @mobinahsansiddiqui21 күн бұрын

    Excellent Explanation, concise, and informative. Thanks.

  • @haleemasadia5225
    @haleemasadia52258 ай бұрын

    Thanks a lot Mam for such well illustration! It helped me a lot as I'm a student of MS biochemistry

  • @rufinamenezes7712
    @rufinamenezes77125 ай бұрын

    An amazing and fun way of explaining. Thank you very much. Please continue it so that many may benefit from your explanations

  • @reconeix
    @reconeix9 ай бұрын

    Excellent, detailed, and approachable presentation. You're a natural educator!!!

  • @supermunheeb
    @supermunheeb Жыл бұрын

    Gonna do this tomorrow this helps me a lot! Thanks.

  • @isaacco2876
    @isaacco2876 Жыл бұрын

    this is a great video! thanks for the info!

  • @brilllance8903
    @brilllance89038 ай бұрын

    perfect video. clear narration. overall 10/10

  • @MichaelSmith-ij2ut
    @MichaelSmith-ij2ut5 ай бұрын

    Hours of lecture and explanation distilled in to 15 minutes.

  • @btssketch937
    @btssketch937 Жыл бұрын

    Thanku so much for this amazing video I really appreciate your efforts ❤❤

  • @gapat8213
    @gapat8213Ай бұрын

    What an incredible science humans has developed. This is something to be grateful for everyday. Plasmid will have the potential to cure all diseases when the special interests will allow it.

  • @WeDream18
    @WeDream18 Жыл бұрын

    Amazing explanation...❤ It covers each and everything you need to know. loves the way she explain.

  • @KatayonZare-uq9cm
    @KatayonZare-uq9cm Жыл бұрын

    That was PERFECT thank you for your explanation❤

  • @christiankemakolam7028
    @christiankemakolam70286 ай бұрын

    protocol simplified and made interesting. kudos

  • @harp6198
    @harp6198 Жыл бұрын

    Thank you very much! This was excellent!

  • @natalia8414
    @natalia8414 Жыл бұрын

    Great video!😊

  • @nurmuhammad4540
    @nurmuhammad4540 Жыл бұрын

    Excellent, thank you so much!!

  • @pingsart7402
    @pingsart74026 ай бұрын

    wonderful demo

  • @salmanashraf8790
    @salmanashraf8790 Жыл бұрын

    Super method of explaining lady (teacher)

  • @michaelokeke7783
    @michaelokeke77834 ай бұрын

    Amazing! I love every bit of this video; so refreshing!

  • @srobles1660
    @srobles16609 ай бұрын

    EXCELLENT WORK!!!

  • @UnKnownVlogger01
    @UnKnownVlogger014 ай бұрын

    Every student like me deserve an advisor like you Mam 😅 Very well explained

  • @isabelasoares1168
    @isabelasoares1168 Жыл бұрын

    Thank you for this amazing video!!! This channel will help me a lot

  • @sebastiantraxler5550
    @sebastiantraxler55504 ай бұрын

    Amazing video!

  • @blacktear5388
    @blacktear53882 ай бұрын

    This was super helpful, thank you so much

  • @shubhamaryan2972
    @shubhamaryan29728 ай бұрын

    great explanation...

  • @alsaqar1
    @alsaqar1 Жыл бұрын

    Best video in this subject 🎉🎉

  • @aamnasajid6570
    @aamnasajid65705 ай бұрын

    the best video I ever saw

  • @03500
    @035005 ай бұрын

    It was amazing, can’t thank you enough 🙏🏻🙏🏻

  • @DiemNguyen-gg8gy
    @DiemNguyen-gg8gy9 ай бұрын

    Thank you very much for the excellent video! It is very helpful!❤

  • @hana-al
    @hana-al5 ай бұрын

    It was excellent !! thank you ❤️

  • @parisahouri
    @parisahouri3 ай бұрын

    The best! Thanks a lot 😊

  • @Brume20
    @Brume207 ай бұрын

    Amazing video.

  • @minahil21
    @minahil218 ай бұрын

    Very well explained mam

  • @user-lp5qe1tv7d
    @user-lp5qe1tv7d4 ай бұрын

    Loved it thanks😊

  • @christopherleubner6633
    @christopherleubner663323 күн бұрын

    Very informative. Highly recommended for molecular bio and genetics students. ❤ ill take my plasmids chilled served neat. 😂

  • @UdhayaBalaKanna111
    @UdhayaBalaKanna1115 ай бұрын

    Just amazing!

  • @maxmordente2218
    @maxmordente2218 Жыл бұрын

    Thank you!

  • @ayeshakhan279
    @ayeshakhan279 Жыл бұрын

    Thank You!

  • @Omar-Khaairy
    @Omar-Khaairy Жыл бұрын

    Thanks so much

  • @Ganesh_lamichhane
    @Ganesh_lamichhane Жыл бұрын

    How beautiful your video like your smile. I liked your video and your smile. Keep smiling and make videos.

  • @taylormarkel1094
    @taylormarkel10948 ай бұрын

    thank you so much!!!!!

  • @kadircanvural6814
    @kadircanvural6814 Жыл бұрын

    awesome! thank a lot

  • @shreeramesh9902
    @shreeramesh99026 ай бұрын

    awesome

  • @md.1276
    @md.12762 ай бұрын

    Don't get me wrong but I was here for science but stayed till the end for her smile

  • @arianevitoriasilvestrehann5140
    @arianevitoriasilvestrehann51405 ай бұрын

    I loved it!

  • @alhaeri1
    @alhaeri13 ай бұрын

    legend

  • @emmanuellaamara1876
    @emmanuellaamara187615 күн бұрын

    what kit did you use, I have been trying plasmid extraction for months now, but I haven't gotten a reasonable result

  • @omkarmore7613
    @omkarmore76132 ай бұрын

    Very amazing with a very pretty mam

  • @rogersundeen4022
    @rogersundeen40229 ай бұрын

    Everybody got this? It's going to be on the test.

  • @ghazalehgolmohammadnezhadk5307
    @ghazalehgolmohammadnezhadk5307Ай бұрын

    What happens if I forgot to wait for 1 minute before the last centrifuge?☹️

  • @wednesdaysfrog8422
    @wednesdaysfrog84229 ай бұрын

    Which kit have you used?

  • @lauraschonborn5633
    @lauraschonborn5633Ай бұрын

    You are so sweet and cool 🎉

  • @richardlange7559
    @richardlange75596 ай бұрын

    Some of that equipment is filthy!

  • @bebolovesshrek

    @bebolovesshrek

    16 күн бұрын

    How

  • @ThatStickyNote
    @ThatStickyNote Жыл бұрын

    Please do not inoculate your bacteria by poking a pipet tip by hand. Hold the sterile pipet tip with sterile tweezers (hold the tweezers in a blue flame for a sec) and then poke the colony on the agar. Also work under a flame or in a lateral flow cabinet.

  • @LabXchange

    @LabXchange

    Жыл бұрын

    Thanks for the feedback! In this video, Molly is working with media that includes selective antibiotic. If she were working without antibiotics, she would have used sterile tweezers. It's important to note that best practices can vary, so you should always check with your PI if you have questions.

  • @badangel842
    @badangel842 Жыл бұрын

    I little bit confuse for Explanation O_o

  • @juxyclips671
    @juxyclips6719 ай бұрын

    Whats her @?