How does DNA look like ? Agarose gel electrophoresis
Agarose gel electrophoresis is used to resolve DNA fragments on the basis of their molecular weight. Smaller fragments migrate faster than larger ones; the distance migrated on the gel varies inversely with the logarithm of the molecular weight. The size of fragments can therefore be determined by calibrating the gel, using known size standards, and comparing the distance the unknown fragment has migrated. This technique can be used to resolve complex DNAs (i.e., genomic DNA)
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Пікірлер: 38
Mam i have watched almost many of your videos with sir while DNA protocol and CTAB preparations. All of these are well. Kindly upload further videos regarding gene mapping and gene constructions. Namsty to you and whole team who did this well.
Very very good and informative video 🙏❤️ Love from Nepal
Really nice😊keep uploading such videos which will help us further👍
Nice video so thank you sir😊
Very very good video with nicely performed experiment and explaination of each reagents and dyes role😊
@ampliconsofbiotech
5 ай бұрын
Thank you so much
Thank you
Also make video on how to interpret the results before and after PCR.
@ampliconsofbiotech
2 жыл бұрын
Sure
बहोत हार्ड .......👌👌
@swapnilvirokar6650
3 жыл бұрын
Kya bat
Excellent Bhava.
@ampliconsofbiotech
3 жыл бұрын
Thanks a lot
Very good...
@ampliconsofbiotech
3 жыл бұрын
Thanks
Helpful video 👌👌
@ampliconsofbiotech
3 жыл бұрын
Stay connected
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Does smears in the result show that we have a lot of contamination in our sample? We take 2 microliters of loading dye and 3 microliters of DNA .. so we have to change the setting of pipette to 5 microliters everytime we load them?
@ampliconsofbiotech
2 жыл бұрын
You can go for Rnase treatment or do some changes in protocol. Concentration of dye and DNA does not affect the quality, smears shows that the problem lies in protocol itself. Either protein or RNA must not have been removed completely.
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Wow very nice dna bands how did you get it please share protocol in detail.. because my plant dna has lot of smearing and bands are not clear and lot of contamination also please help me
@ampliconsofbiotech
2 жыл бұрын
Share your results on +919834128616 we'll help you with the protocol.
@madhurrathore4021
2 жыл бұрын
@@ampliconsofbiotech ok thank you
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Could you please help me with removal the smear and contamination..even after purification... Thank you...
@ampliconsofbiotech
Жыл бұрын
Which protocol you used for isolation and for which plant sample?
Why smeared DNA ladder occur?
@ampliconsofbiotech
5 ай бұрын
If agarose is not dissolved properly, or ladder may be older/contaminated.
What do bands tells us ??
@ampliconsofbiotech
Жыл бұрын
The size of the DNA molecules can be estimated by comparing the position of the bands on the gel to a standard ladder of known sizes. The smaller DNA fragments will travel further down the gel, while larger fragments will move more slowly and remain closer to the top. The intensity of the bands can provide information about the amount of DNA present in the sample. More intense bands indicate a higher concentration of DNA.
White light wale band hai kya
@ampliconsofbiotech
Жыл бұрын
Yes