Gene knock Out | Neomycin | G418- Casette I Experimental | PYQ included | CSIR-NET JRF |GATE | DBT
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Gene knockout (KO) is a technique by which the genomic DNA of a cell or a model organism is perturbed, so that the expression of a specific gene is permanently prevented.
please watch this video for more detail
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Ma'am, keep up your great work. Best wishes.
Loved your video maam❣️I was searching for this video.
Thank u mam Your way of teaching superb And you make tough topics easy magically
Thank you mam for explaining this concept 👍🏻
You are excellent superb fabulous fantabulous awesome Teacher a big big big thank You dear mam
2 minutes silence for those students who don't find this type of fabulous Explaination😁😁
@user-ud3yf7sf8u
4 ай бұрын
Right
Literally ma'am, I was just searching on this topic. And I got your notification
@BaaYo
2 жыл бұрын
Glad it was helpful!
Home work pura hua mam 😀 Thank you ❤️🙏
It was very nice video mam , hamesha ki tarah👌👌👌🙏🙏
@BaaYo
2 жыл бұрын
Thank you Mahesh
Answer will be B ,C ,D respectively , thank you ma'am for this awesome lecture and saving me before exams 🥲
Best teaching
Best explanation
Thanku mam for this class
Thank you ma'am ❣️
Thank you so much Ma'am for the informative video. I had a question regarding transfer of gene as said after electroportion vector gets to the genome and the genes get switch, so how the tk+ gene went in the ectopic and not in ideal cell...
Very nicely explained. Thanks ma'am
@BaaYo
2 жыл бұрын
Thanks you
Nice nice nice, thank you so much🥰
@BaaYo
2 жыл бұрын
😍
Well done mam
Thank you maam.
Thank You ma'am for these series. I literally afraid of the unit 13. Finally it reduces some.
@BaaYo
2 жыл бұрын
You can do it!
@Raj-mr9vg
2 жыл бұрын
Bro you are good doctor lol
Wonderful explanation mam... Answers of the given sequence of questions will be 2,3 and 4 respectively
@BaaYo
2 жыл бұрын
🙂
6-b,140-c,135-d
Wow mam Best explanation 👌🏻👌🏻👌🏻❤❤
Thanks🌹🌹 mam
@BaaYo
2 жыл бұрын
❤️
A. O. A Dear respected Mam
Thank you ma'am 🙏
@BaaYo
2 жыл бұрын
Tripti Please share with your batchmates also
@triptisingh8137
2 жыл бұрын
@@BaaYo sure mam
Thanku
@BaaYo
8 ай бұрын
🤩
6-D, 135-C, 140-B. Thank you mam😍
@BaaYo
2 жыл бұрын
try again.. their is some mistake
🙏🙏💕💕
6-B ---bcoz cell survive in the presence of G14 & ganciclovir 140-C :- no transition 135 - D :- not crrct bcoz it is non homologous define Thank you for crrecting me mam😍 again video dekha acche se thrn qusn solve kiye..phle jldbaaji krdi thi😋
@BaaYo
2 жыл бұрын
Perfect ❤️👏👏👏👏👏
@hi-bg6uv
2 жыл бұрын
@@BaaYo 😄
Mam please explain resistance and sensitivity
6.b 104.3 135.2 and 4
2 and 4 are correct answers
Ma'am.. I've a doubt.. in ectopic insertion why the "tk" gene got inserted along with the gene of interest ..
Assalam o alikum! Mam plz crisper Cas9 par b Aik vedio bnaye plz
6-b 134-3 135-4
Question1 :- B Question2 :- c Question3 :- D
Please one vedio on monthly calibration of FTIR Nicole iS5 thermo performance
@GhulamHussain-bw9tj
2 жыл бұрын
Pls specify its application. Which product u r analysing in ftir?
@BaaYo
2 жыл бұрын
noted
6B 140C 135-4. These are the answers maam. Are they correct? Thank you so much maam for ur efforts🙏❤️
@BaaYo
2 жыл бұрын
Mam I do not understand the last few steps having some confusion Please clear I'm preparing for an exam Confusion is that you said at 20.13 that one gene is delete and 1 is functional i understand about the deleted gene but which gene is functional here I mean we take only 1 gene here than which one is functional getting confused here please clear
Transgenesis or Geneknock out mai kya distiction h mam ?
Mam G148resistance ke liye kya responsible he????
Ques: 6_D, 140_C, correct or not? 135 nhi samajh aarha ma'am help me
6- B, 140- C, 135- D. Mam check the answers
@BaaYo
2 жыл бұрын
Correct 👏👏
@akhilthota3860
Жыл бұрын
@@BaaYo i dint understand why 140 - 3 is the correct answer. Can you please explain mam
@rononoazoro1808
Жыл бұрын
@@akhilthota3860 he made a construct that was sensitive to neomycin ( so the neomycin gene will be outside of the homologous terminal region and will be left outside even the recombination is taking place ) and resistant to ganciclovir ( meaning the TK gene will also be outside) hence answer is 3 as both the gene will be outside of the homologous terminal regions
@manharkrishnaojha5252
Жыл бұрын
Ma'am gene targeting topic pr video bna dijye
Add english subtitles guys...
Ques1 (2) Ques2 (3) Ques3 (4)
@BaaYo
2 жыл бұрын
well done
1:-B 2:- c 3:-d
What's the use of tk gene in vector mam
@BaaYo
Жыл бұрын
It is helping in selection of negative cells. Jin cells me random integration hai vo tk gene express krti hai
@user-ob2tp8pw5p
11 ай бұрын
Thank you mam
Ma'am can't understand the last question,why the answer is option D? Please help
@BaaYo
2 жыл бұрын
They have asked for incorrect statement. did you notice that?
@rachanasinha2113
2 жыл бұрын
@@BaaYo Yes ma'am. I am not able to understand the neo gene insertion site.
6. 2
140 ) 3
Mam 6. Answer b kaise ho rha please explain
Maam 140 no question 3 ans kaise hoga
135. 4
Mam, tk ko outside kyo rakha hua h?
@BaaYo
2 жыл бұрын
correct and ectopic insertion ko identify krne me help kreta hai . Agar vo cassette ke beech me hoga to vo bhi neo ke sath switch krega aur cell ke genome me integrate ho jayega aur express hoga. to cell ganciclovir sensitive ho jayegi. Hope its clear
@maheshswami5226
2 жыл бұрын
@@BaaYo thank you pyari mam🥰🥰🙏🙏
Hlo didi aapse kuch guide leni thi
@BaaYo
2 жыл бұрын
btaiye
@harishbhatia3754
2 жыл бұрын
@@BaaYo muje bhul gye kya didi
can we contact you