GENE CLONING In Detail - Part 3 | CSIR | Replica Plating
Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid selective growth media are inoculated with the same colonies of microorganisms from a primary plate, reproducing the original spatial pattern of colonies.
To understand the process easily, please watch this video.
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Пікірлер: 68
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Thank you Ma'am. Stay healthy and stress-free.😊
Difficult topic is explained with so much ease.... With regards Keep it doing , respected ma'am 🙏
Beautifully explained 😍 thank you Ma'am ✨
Nicely ,lucidly explained ,mam thank you.
Finally mam ab apki video aaney lagi h.... 💐thnku mam🤗
Great lecture mam ,tq so much💖💖💖🥰🥰🙏🙏
Very nice conceptual lecture 😃
Thanks for a nice class
Bahut miss kie mam hm apko.... Itne dino se... Aapke jaane se mujhe hard study krni padi aap padhati hai to me easily samjh jaata hun or study easily ho jaati hain
After long time on your on tube channel mamm am waiting for your new vedios please don't break the continuetion ❤️
Amazing
Amazing mam ...🤗🤗
Thank you so much maam❤️
Thanku soo much mam, ❤️
Thanks mam
Nice to watching back to back lectures. Awaiting for spectroscopy related lecture. As you have taught previously but need some more details.
@BaaYo
2 жыл бұрын
Noted
Nice
Thankyou so much mam to this lacture ....option B correct☑️
@BaaYo
2 жыл бұрын
no, answer C is correct
Mam can you please explain why the answer is c. I have understood the concept but could not related how and why the answer is c.
Thanks ma'am
@BaaYo
2 жыл бұрын
Thank you too
Please upload pcr with different types and primer design with full pcr explanation
thank you maam
@BaaYo
2 жыл бұрын
Thank you too
Ans 3 mam ....first use leu- his+ then use leu-his- in replica plating method
Ma'am answer is C, but 1st plate we will get the self ligated ones, and in replica plate the recombinant ones.
Thank you mam for this beautiful lecture. Answer is B
@BaaYo
2 жыл бұрын
Right answer is 3
Mam please make video on HPTLC chrometograhpy..
Mam plz explain that how the correct option is c?
Option 3 is correct
Maam how the answer will be C? Because in 1st plate we have to take the intact one naa which is Leucine..plz explain this 🙏
Thank you Ma'am for this amazing lecture. The answer for the Question that you asked is Option (B).
@BaaYo
2 жыл бұрын
answer 3
I like you so much🤩
Ans 3..plz checked mam
@BaaYo
2 жыл бұрын
Yes Pooja
@dubeyp5555
2 жыл бұрын
Can you explain Pooja??
Thank you mam for this video, Mam please make video on Capillary electrophoresis , please
@BaaYo
2 жыл бұрын
Noted
Plz es qustion ka detail me samjha do jisne 3 ans kiya hh
Answer will be -B mam ...at frst Leu marker will be add bcz it fixed in both self ligated and Rec. Cell...then replica plating will done by adding both leu and his. marker
@BaaYo
2 жыл бұрын
correct answer is C
@rutujadandekar5622
Жыл бұрын
@@BaaYo since many people are confused with option B & C it is expected from you to give explanation
You looking very beautiful 🥰🥰
Ans 2
How to obtain a clone of specific gene?
Mam yaha transformed cell ka poocha hai to wo to already de rkha hai ...hmein sirf his- ka pta krna hai .....to answr is C.....aise hi hai na mam??
@Thesiblingshow4
2 жыл бұрын
Explanation is not clear but yes correct answer is C option
Answer 3
While performing replica plating how can we use dead bacterial cells ( recombinant transformed ) for further experiments ? If these cells don't have tetracycline resistant gene then they will die ..
@StudyBits3560
5 ай бұрын
Good question ! We usually have large stock .so. After knowing through replica - plating we mark the stock as competent cell stock .they can be used for further experiments.
I did not understand why the answer is c?
Mam will you complete this unit in Unacademy plus?
@BaaYo
2 жыл бұрын
yes
Maam please ye clear ky dy. 1st plate sy tu hm. NY transfer kr diye to waps kaisy ayengy is k pass.
Aapse contact kese kru
3
Ma'am please explain the answer I'm not understanding this! 😓
Mam bioenergetics ka koch topic apka missing hai.
@alizain8555
2 жыл бұрын
Class 12
Hlo didi kuch puchna tha aapse
Ans 3
3
3