No words to describe how much this video informative for me.

it is important not to move the packed column (like up and down) to avoid column cracks

very helpful video🙌

Its also best to wet-pack the column. That reduces the odds of cracks or unevenness, and makes it quicker to pack. It is imperative to pack it properly by slapping the column with a rubber tube or anything of that sort.

Thank you.please how much silica gel need to weight in wet and dry method? Thank you

what air pressure do you use?

well designed , planned & presented. you all get an 'A'

@Seorful

3 жыл бұрын

Not with that background music...

How much loading capacity of compound??

Dude, the column isn't moving, it's dancing... lol

WHY THE MUSIC

this is not flash chromatography......

OSHA would like to know your location

Where’s your lab coat?😅

This is so bad

@crabcrab2024

2 жыл бұрын

I am very glad someone else noticed it.

@VKA_IITian

Жыл бұрын

What is bad about it? 🧐

@yellosa

Жыл бұрын

@@VKA_IITian Chronic (long- term) exposure to hexane in air is associated with polyneuropathy in humans, with numbness in the extremities, muscular weakness, blurred vision, headache, and fatigue observe

@chrish.942

Жыл бұрын

..d in industral workers working in factories reeking of hexane for years on end. If your lab reeks of hexane i can understand the concern, but with the amounts used here, you're unlikely to get anywhere near the hundred ppm exposure limit at any point. I'd be ever so slightly more concerned about DCM bt even there it's not that big a deal, maybe move it to the fume hood on an exceptionally hot day.

@grebulocities8225

8 ай бұрын

Yeah, I also wouldn't worry about occasional exposures to small amounts of hexane like this. If you are still worried, just substitute heptane - it will work identically, but does not have the same neurotoxic effects and is also somewhat less volatile.

“Ah-loo-ent”...eluent is pronounced “EL-you-ent”