It's amazing that this channel makes it possible to practice straight away, making it very easy for us to work in the laboratory. Thank you🙏

@MicroChemsExperiments

8 ай бұрын

Thank you

I can never find someone explains better than you did . Thank you

@MicroChemsExperiments

3 жыл бұрын

Hassiba Hassani, Awww, How sweet. Thank you so much for your comment. Be with us. ^_^

I really appreciate your effort for showing as such an amazing virtual lab. All the description was exactly in touch with each and every moments of the whole experimental framework. # as a reminder, please present as how wijs reagent is prepared in lab. Thanks.

@MicroChemsExperiments

2 жыл бұрын

Okay. Thank you so much.

@hernandorodrigues9259

Жыл бұрын

@@MicroChemsExperiments Como saber se o carvão ativado está acima de 800% de iodo

This is the best channel I have ever seen, thanks for your effort ❤

@MicroChemsExperiments

2 жыл бұрын

Thank you so much. Where are you from Ma'am, may I know?

@esraamossad3970

2 жыл бұрын

@@MicroChemsExperiments Egypt

@MicroChemsExperiments

2 жыл бұрын

@@esraamossad3970 You can contact us in Facebook also. Link is given in the description section.

@esraamossad3970

2 жыл бұрын

@@MicroChemsExperiments I have already followed you on Facebook, thanks MicrChem ✌

@MicroChemsExperiments

2 жыл бұрын

@@esraamossad3970 Thank you Ma'am. Stay with us.

Great presentation! Please continue the good work. Knowledge is for sharing.

@MicroChemsExperiments

3 жыл бұрын

Thank you so much. Stay with us.

I have learn a lot through this your channel sir Thanks for bringing value to this noble channel. I appreciate your effort.

@MicroChemsExperiments

3 ай бұрын

Glad to hear that. Thank you

You are awesome! Thank you for sharing!

@MicroChemsExperiments

2 жыл бұрын

Thank you for supporting us.

Ihave iodine mono chloride in the labe but if iuse it it doesnot give me the good reading for the blank low readeing what can ido any way to increse the wigis solution strength

Hi, thanks for making this video! It is really helpful. I have been doing my experiment about how the number of times heating the corn oil till boiling will affect the oil’s iodine value and I realized that as I did titration, there was a layer of oil-like solution at the bottom of the flask. Do you know anything about this and why does it happen? Because it has became really obvious and disturbing since the oil darkened as the heating number increased.

Hi MicroChem´s, can i make this determination of iodine value in activated carbon??

please how can Ido iodine value in oil by automatic titration machine ihave thermo brand t940 orion this test is redox and back titration but cant to do the steps can any one guide me

Hello, may i ask what were the molarity of the Wijs solution and carbon tetrachloride used here? I would like to see how i can change the calculations if i am using different concentrations

@MicroChemsExperiments

2 жыл бұрын

Wijs (iodine monochloride) solution: 0.1 mol/L Carbon Tetrachloride: 99%

it's good job.thank you very much

Thanks for your wonderful and passionate video. It's really useful for my work. I wonder some point that could you explain for me, please. 1. Why we certain use Carbon Tetrachloride in this experiment. Can you explain in detail the reason why you use Carbon Tetrechloride? Can I replace it by other substane? 2. I wonder if you put Potassium Iodide crystal around the stopped? Why don't we use the solution of Potassium Iodide? 3. In the phase that you pour distlled water into the flask, you titrate the flask with Sodium Thiosulfate until the yellow is lighter before you add the starch solution in the flask. So I wonder the reason why you must to titration before adding the starch solution. Is there any theory in this phase? I really want to know. I truly appriciate your support and I am looking forward to hearing from you. Many thanks

If the blue color keeps coming back within the 30s, what could be the problem? Right after turning milky-white, the blue color returns. This happens repeatedly even after titrating more of the thiosulfate

Thanks for sharing with us your valuables information

What an excellent explanation ❤❤ thank you so much

@MicroChemsExperiments

Жыл бұрын

Thank you

Can please ask why are we still multiplying with normality if we have converted to mass already?

I like your explanation and demonstration Thanks

@MicroChemsExperiments

3 жыл бұрын

Thank you too. Stay with us.

hi , I enjoyed the video but shouldn't the sample amount been pipette out instead of poured in because the majority of the sample was on the side of the flask not at the bottom

Thank you so much for your important video❤❤❤

@MicroChemsExperiments

9 ай бұрын

It's my pleasure

Before do any lab experiment i search it your channell.it is very usefull to me. Though i get wuections😉😊😊😊😊

@MicroChemsExperiments

3 жыл бұрын

Kasthuri Kk, It's our pleasure ^_^ We are planning to make videos for almost every laboratory tests. Just be with us.

How to prepare wij's solution?? 50ml iodine monocloride desolved in how many acitic acid in ml?? Please🙏 reply

Appreciate your effort on preparing such useful videos.

@MicroChemsExperiments

3 жыл бұрын

Thank you very much. Stay with us.

My question​ is not about AOAC but about AOCS methods, that before weighting the sample, sample must adjust temperature to 68 -​71 celsius deg. I wonder what is the purpose of sample temperature​ adjusting?

@MicroChemsExperiments

2 жыл бұрын

Chemistry is all about chemical reactions. Chemical reactions takes place differently at different temperatures. So the adjustment of temperature is very crucial for any chemical analysis of sample.

All tests which i need in solvent plant laboratory testing, all tests are there on your channel thank you so much

Hi love the video！Just wanted to ask why put the flasks in dark for 30 min？will the time of this have an effect on the titration?

@MicroChemsExperiments

Жыл бұрын

This is done to allow reactions of some light sensitive chemicals.

Also when I carry out the procedure, there is a purple coloured layer at the bottom of the solution in the blank and a pink coloured in the sample, do you know anything about this?

@MicroChemsExperiments

Жыл бұрын

Nothing noticed like that. Prepare chemicals and reagents freshly and try it again

i took 0.216 gm sample of groundnut oil its value came 93. isit in expected iv range or this answer is wrong

1. Quality testing of raw as well as finished products of confectionary. 2. Preparation of: 1. candies, 2. Caramel, 3. toffees. 3. Collection of various types of confectionary packages. 4. Determination of sugar in confectionary product by saccharometer. 5. Determination of refractive index of sugar - solutions of different consistencies

@AbhishekSharma-mo3nb

2 жыл бұрын

Pls make vedios on this tests

@MicroChemsExperiments

2 жыл бұрын

Okay.

@AbhishekSharma-mo3nb

2 жыл бұрын

Thank you so much

@MicroChemsExperiments

2 жыл бұрын

@@AbhishekSharma-mo3nb you are most welcome

Informative video tutorial.

@MicroChemsExperiments

Жыл бұрын

Thanks

thank you

@MicroChemsExperiments

Жыл бұрын

You are welcome

You are just superb!! Can you plz make a video on sugar determination from carbohydrates...i mean fehling solns..at which point we can consider the end of the titration.

@MicroChemsExperiments

3 жыл бұрын

Astha Jain, Thank you. We have already started making video for Sugar determination. Stay with us.

Which oil u have used in this pls tell

Thanks. Was helpful for exams in my masters Thanks a lot

@MicroChemsExperiments

Жыл бұрын

Thanks

Thank u so much ...☺️

@MicroChemsExperiments

3 жыл бұрын

You are most welcome.

Nice your analysis

@MicroChemsExperiments

3 жыл бұрын

Saroj Sahu, thank you for your comment. Stay with us.

Thank you for this vid. I just want to know, how do you handle these chemicals as waste after the analysis?

@MicroChemsExperiments

11 ай бұрын

We have third party company to handle this

Can you guide me how to calculate ! Especially the blank thinks

this was so well explained💝

@MicroChemsExperiments

11 ай бұрын

Thank you

Ma'am I have a simple question what is purpose of this test or what does tell us this test?

Very informative video

@MicroChemsExperiments

Жыл бұрын

Glad you think so!

This was great work. One question, wouldn't the KI crystals placed on the top interference with the reaction?

@MicroChemsExperiments

3 жыл бұрын

Aksania Morris, thank you for your comment. No, Addition of KI wouldn't interfere the reaction. In AOAC it ia said to add KI.

@aksaniamorris3042

3 жыл бұрын

@@MicroChemsExperiments ok thank you

@MicroChemsExperiments

3 жыл бұрын

@@aksaniamorris3042 Welcome. Thanks for being with us.

@Cookie-wt4uz

2 жыл бұрын

Actually the KI helps to liberate the unreacted I from Wij's solution

@newsofislam1284

Жыл бұрын

@@MicroChemsExperiments You can add 10% KI solution 15 ml into flask,

Hi! where is the sample based on the expecetd iodine value table from? I cant find it on the internet

@MicroChemsExperiments

3 жыл бұрын

The expected value of different samples is given nowhere at a place. You have to find one by one. Say for example, you want to test iodine value of soybean oil. Then search on google by "Iodine value of soybean oil". ***Google will show you normal iodine value of soybean oil. ***Another source is Published Journals about "Studies on Soybean Oil". ***You can also find the normal iodine value range of your country's "Standard Of Soybean oil" Search the value following above 3 ways and get the idea. NB: If not works above steps, then test your sample thrice considering medium, high & low value. Then you will get an idea about the expected Iodine value of your sample. Thank you.

@sahilrohilla3863

3 жыл бұрын

Constanza Uribe Cejudo

@sahilrohilla3863

3 жыл бұрын

Heyyy , i can help you in your exams and assignments , i am a medical expert .

I have been given unknown kind of fat or wax maybe but the point is this sample is in solid nature, how will I predict it's Iodine value so that I'm able to weigh the supposed weight of the sample?

@MicroChemsExperiments

Жыл бұрын

Go for the first trial taking any amount of sample which will give you an idea about the iodine value (approximate). After getting the idea, go for the second and final trial taking sample weight according to the iodine value you got from the first trial

Thanks

@MicroChemsExperiments

Жыл бұрын

Welcome

how does heat affect this experiment? how would I heat the solution after keeping it in the dark if I want to check temperature affects on iodine value?

@MicroChemsExperiments

3 жыл бұрын

You can set an experiment by applying heat and let us know the result. But I thing heat will affect the test result because iodine will be removed from the flask during heating and a false result will come out from your experiment.

Can you please help me with a soft copy of the method Thanks

Thank you for sharing. may ask question why add KI at neck of flask when during preparation of sample and blank?

@MicroChemsExperiments

2 жыл бұрын

It is said to add in AOAC.

@umeshyadav-xj2uz

2 жыл бұрын

@@MicroChemsExperiments 👍👍

@waytohellgamers5201

2 жыл бұрын

Bcoz iodine vapours doesn't go outside of iodine flask

@newsofislam1284

Жыл бұрын

You can add 10% KI solution 15 ml into flask,

Hi. Why did you not add 10-15% KI b4 stopping with water? Thank you!

@newsofislam1284

Жыл бұрын

You can add 10% KI solution 15 ml into flask,

Its It's very good to see experiment very clearly I have thought our chemists by showing the video... many thanks... Please update USM UNSAPONIFICATION MATER ALSO

@MicroChemsExperiments

3 жыл бұрын

NUTHAKKI RAJESH, okay we will work on unsaponification mater as early as possible. Be with us.

@rajesh7999

3 жыл бұрын

@@MicroChemsExperiments thanks

@MicroChemsExperiments

3 жыл бұрын

NUTHAKKI RAJESH, This video will help you. Please take a look. kzread.info/dash/bejne/dqSbz6qxXcfSYaQ.html

Hi could you please let me know any alternative for CCL 4 ( apart from chloroform ). Thanks

@MicroChemsExperiments

2 жыл бұрын

No replacement to be known for CCl4 other than Chloroform

@kavindibalasinghe8144

2 жыл бұрын

@@MicroChemsExperiments thanks alot for the reply

nice

@MicroChemsExperiments

3 жыл бұрын

Shrikrushna Chaudhari, Thank you very much. Be with us.

Where Didi you get this 12.69

Iodine molecular weight is 126,90477 g/mol . Why was this (12,69 g/mol ) written in the formula?

Hi, thank you for the video! But I have a question. What is the purpose of adding potassium iodide crystal? And can you quantify the amount of potassium iodide crystal added around the surface of the stopper? I'm a student planning to do this experiment in schoolwork.

@MicroChemsExperiments

Жыл бұрын

Potassium Iodide helps to release Iodine. You can add 500mg into the flask

@IvyLin-is8bi

Жыл бұрын

@@MicroChemsExperiments Thank you!

Hi, what's the concentration of wij's solution did you use?

@MicroChemsExperiments

Жыл бұрын

1.623%

Blank me starting me kya liya tha befor adding ccl4

Why don't we add starch indicator in the beginning of titration? What only when the colour of the solution becomes lighter?

@MicroChemsExperiments

2 жыл бұрын

It is a two spet titration. More accuracy is achieved by adding starch when the colour is faded out.

what is the difference between this method and ASTM D4607-94 method? Can I use this method for activated carbon?

@MicroChemsExperiments

3 жыл бұрын

No. Our method is for foods and oils. We will prepare method for activated carbon later. Stay with us.

@user-df5is8jy6n

3 жыл бұрын

@@MicroChemsExperiments oh thank you very much, looking forward to it.

@MicroChemsExperiments

3 жыл бұрын

@@user-df5is8jy6n Welcome.

What is the unit.? Of IV in this calculation.?

Thanks for your knowledge sharing... please make a video on "urease activity of soya bean meal"...

@MicroChemsExperiments

3 жыл бұрын

Ok...we will do it soon. Stay with us.

Where can I buy or how can I get the AOAC manual for the different methods? I'll still keep watching you! 🙏🐾

@MicroChemsExperiments

2 жыл бұрын

You can purchase it from Amazon.

@bishow8781

2 жыл бұрын

Full meaning AOAC

i have prepare Wij stock solution using 50g ample in 500ml (makeup)acetic acid and for working solution i have take 80ml of stock solution and makeup with 500ml of acetic acid is this correct

@MicroChemsExperiments

Жыл бұрын

We will make video for the presentation of wijs solution soon

hello! what is the solvent used fir wij's solution? can water be used?

@MicroChemsExperiments

Жыл бұрын

use acetic acid as solvent

It was great work. I have a question? Will there be any problem if I use saturated KI solutions?

@MicroChemsExperiments

2 жыл бұрын

You can use saturated KI solution

@user-jk6yb4wz3s

2 жыл бұрын

@@MicroChemsExperiments ♥️♥️♥️

@user-jk6yb4wz3s

2 жыл бұрын

@@MicroChemsExperiments Can you give me your number, please?

@MicroChemsExperiments

2 жыл бұрын

@@user-jk6yb4wz3s knock in our Facebook page. Link is given in the description section

what does the potassium iodide do, is it needed for the experiment

@MicroChemsExperiments

Жыл бұрын

Yes. It is needed

thank you for making a test video tutorial like this. the video is very clear and easy to understand. It's just that there are some points that explain it too quickly, so I feel that there is something missing, but it turns out that the explanation is just too fast.

@MicroChemsExperiments

3 жыл бұрын

@Nadia Qonita, You are most wecome and thanks to you for your observations. We will try to make our videos more clear.

Can you give me the "Determination of Oil Analysis" manual if I need it?

@MicroChemsExperiments

2 жыл бұрын

We do not provide any document

What is filter paper number used to prepare 1 percent starch solution?

@MicroChemsExperiments

Жыл бұрын

Number 1

@Athokshaya_V

Жыл бұрын

@@MicroChemsExperiments can we use whatman no. 4 filter paper?

0.21 gm sample h to expected iv groundnut oil me kitna hona chahiye

I watch iodine value analysis video many time.which is very good & clear.but i want to know without putting iv flash in dark test can be done?

@MicroChemsExperiments

Жыл бұрын

Putting iv flash in dark is necessary

Hii! first of all, your videos are very helpful. But may i ask something, why is blank titration necessary in the this determination(iodine value det.)? I hope you answer me ,thank you so so much

@MicroChemsExperiments

3 жыл бұрын

Blank titration is needed to subtract reagent reading.

@biancaysabelvicente215

3 жыл бұрын

@@MicroChemsExperiments Thank youu Ma'am/Sir.

@MicroChemsExperiments

3 жыл бұрын

@@biancaysabelvicente215 You are most welcome.

@sahilrohilla3863

3 жыл бұрын

Heyyy , i can help you in your exams and assignments . I am a medical expert.

After preparing sample in IV flask And during kept in dark for 30 minutes why we use KI Salt on the top of the cap of the flask ??

@MicroChemsExperiments

3 жыл бұрын

Goutam Gupta, Thanka for your comment. According to AOAC method, you need to add KI. KI crystal is added on the top of the cap. The reason is: it prevents the transportation of volatile substance from the flask to the outside environment. Rather KI crystal absorb the volatile material from the cap and enter into the flask during washing with water.

@goutamgupta8816

3 жыл бұрын

@@MicroChemsExperiments thank for clearing doubt .

@MicroChemsExperiments

3 жыл бұрын

@@goutamgupta8816 you are most welcome. Stay with us.

Super

@MicroChemsExperiments

3 жыл бұрын

Thank you

Thanks for the video, But if result 38 for olive oil and compared with reference rang and show less than from rang what writing comment?

@MicroChemsExperiments

2 жыл бұрын

The quality of that oil is not good and maybe mixed with other cheaper oil.

Who the hell disliked. Idiots...perfect demonstration. Pls low noice back ground sound adjust. We lv your channel.thanks. plc to hplc aflatoxin analysis

@MicroChemsExperiments

3 жыл бұрын

@Kasthuri Kk, Ha ha ha...1 dislike among 89 like is negligible. They have rights to express their opinion. So plz don't argue with them who disliked. What you can do, you can love the channel more & more. We will make the background sound lower from the next video. Which aflatoxin (among B1, B2, M1,M2,G1or G2) do you want video for?

@Titi-je3ff

3 жыл бұрын

B1,B2,G1,G2 now im currently initiatimg in my lab sri lanka.if you can pls do other also.it will be really usefull.u guys are amizing.

@MicroChemsExperiments

3 жыл бұрын

@@Titi-je3ff Okay. We will make it as early as possible. Thank you for your comment.

Hi Mam Is it possible to analyse cholesterol content in edible oil by volumetric titrations..??? Without chromatography or spectroscopy plzz reply me mam...

@MicroChemsExperiments

2 жыл бұрын

We will upload video later.

Why uou mention 12.69 Can You explain plz

Please how to prepare Ipa lactic acid solution for sv test of wheat flour.

@MicroChemsExperiments

Жыл бұрын

Did you mean sedimentation test of wheat flour?

Hello, what oil is used in the sample oil?

@MicroChemsExperiments

2 жыл бұрын

Coconut Oil

Mam jo dark m rkhte ho vo only 30minutes which type of dark you use plzz tell

@MicroChemsExperiments

2 жыл бұрын

I used cabinet where no light can enter.

Nice good explanation,i have one question,Why we kept sample in a dark place for exact 30 minutes.

@MicroChemsExperiments

Жыл бұрын

To allow the chemical reaction satisfactorily that could be halted in sunlight

how to test charcoal to see if it is activated above 800% iodine

@MicroChemsExperiments

Жыл бұрын

We never tried with charcoal. So no idea. You can try with this method.

Why initial value sample of 25.6 ml and blank 2.7 ml take in burette

@MicroChemsExperiments

3 жыл бұрын

We started titration of sample first. In this case, initial reading was 25.6 and the titration was finished when the final burette reading was 39.2. We refill the burette again and start blank titration when the initial burette reading was 2.7 and finished when the final burette reading was 39.1. NB: we did not refill the burette completely thats why the initial burette reading was started at 25.6 and 2.7 for sample and blank. There is no specific reason for it. You can start both titrations (sample & blank) from 0, 1 or any other initial point. There is no limitation in it. It's up to you that from which point you want to start.

Any reference book for this experiments

@MicroChemsExperiments

3 жыл бұрын

Rakshinda Sayed, Yes. This test is strictly followed by the method 'AOAC 920.159'.

Hi, I was wondering why is it so important that it should be closed immediately after pouring the wij's solution. By the way, this is a great video it has helped me so much. If you have any other papers, videos, or references about determining iodine value in oils by wij's method I would be thrilled If you could reference it. Again thank you.

@MicroChemsExperiments

3 жыл бұрын

Ekin Gursoy, Thanks for your comment. Actually, when Wij's solution is added, it reacts with Iodine present in the sample to form slightly volatile compound. If we left open then loss of iodine may occur. That's why we keep the flask closed immediately after adding carbon tetrachloride and Wij's solution. Other two references for this test are: IS-548 and FASSAI MAnual.

@ekingursoy1779

3 жыл бұрын

@@MicroChemsExperiments thank you very much

@MicroChemsExperiments

3 жыл бұрын

@@ekingursoy1779 You are most welcome.

@khemist2631

3 жыл бұрын

@@MicroChemsExperiments Great video, very interesting to watch. However, why was the iodine crystals added around the stopper after closing?

@MicroChemsExperiments

3 жыл бұрын

@@khemist2631 It is added to set a barrier for passage of gas from the flask to the outside during the 30min of the reaction time.

What is the 12.96 in the formula.Can you tell me.

@MicroChemsExperiments

2 жыл бұрын

Calculation factor

For the blank , we should to find iodine value also ?

@MicroChemsExperiments

Жыл бұрын

Not iodine value actually. Blank reading represents the chemical error for iodine value test

At the end point pink color emulsion kya usko bhi milky bnana h

@MicroChemsExperiments

3 жыл бұрын

Milk color emulsion along with white solution will form at the end of the titration.

Why we need to weigh the sample?

@MicroChemsExperiments

Жыл бұрын

Sample weight is required for the calculation

Please post a Video for Determination of TVBN in Dried Fish Meal sample AOAC

@MicroChemsExperiments

Жыл бұрын

Noted

I have 2-3 questions,how can i contact you? Plz ans..

@MicroChemsExperiments

Жыл бұрын

Already answered. Do this test exactly as done in this video

AOAC Official Method 920.159 *lodine Absorption Number* of Oils and Fats, this is the method that I referred to.

@MicroChemsExperiments

2 жыл бұрын

Yes

If we don't know iodine value limit of any sample then how much weight should we have to take for calculating I.V.

@MicroChemsExperiments

2 жыл бұрын

You can get idea of iodine value for specific oil from google and journals.

@user-eu6ff2dm9m

6 ай бұрын

for groundnut oil what weight we should select @@MicroChemsExperiments

I need a blechability test for cruid oil ,plese share

@MicroChemsExperiments

2 жыл бұрын

Noted.

Very useful information, however one issue tho, you do not heat a Volumetric Flask!

@MicroChemsExperiments

Жыл бұрын

Noted

There's a mistake in your analysis the equivalent weight of sodium thiosulfate is 248.16g so if we want to make 0.1 N Sodium thiosulfate we need 24.6g not 2.48 g

In here which is value of 12.69???? How u find it?m

@MicroChemsExperiments

11 ай бұрын

It is a calculation factor given by the reference method of AOAC.

can I replace carbon tetrachloride with something else ?

@MicroChemsExperiments

2 жыл бұрын

No replacement is applicable here

@harshitakaushik9058

2 жыл бұрын

You can use chloroform.