i have been looking for this for so long thank you so much

@MicroChemsExperiments

3 жыл бұрын

Mike tyson, It's pleasure. Stay with us

@abdullahaljobair5189

11 ай бұрын

​@@MicroChemsExperiments is it 28.05 or 28.5?? Where does it come from in the formula? Is it a factor??

Thq so much for a wonderful video...Tmrw i have to explain it and guide my frnds to perform it in laboratory...so this video helped me a lot...🥰 Thq so much

@MicroChemsExperiments

2 жыл бұрын

You are most welcome. Stay with us

Very informative video, now my all doubts regarding to this practical are clear.. thank you so much ❤️

@MicroChemsExperiments

2 жыл бұрын

Thank you. Stay with us.

Thanks for the great video

Very perfect lab work👏👏👏

@MicroChemsExperiments

2 жыл бұрын

Thank you very much!

when i did this experiment, i mixed 3.4g KOH+ 2ml distilled water+rest of ethanol to make upto 100 ml volume(0.5 N solution). I made this ethanolic KOH using directions I found on the web. Then I directly dissolved 1g oil sample in 50 ml of prepared mixture. It got clear only in 5 minutes. I prepared 0.5 N Hcl by adding 10ml in 250 ml water(37% Hcl). When I titrated, I was getting a very large saponification value(about 500). The formula I used is [(blank titre-sample titre)*56*normailty of KOH]/weight of sample. Can you tell me what am I doing wrong? And what is the purpose of the methods u have used like use of aluminium foil and initially boiling koh with ethanol?

You provide us more and morer video which is very important for our laboratory work But........ I still waiting for analysis video of sugar percentage...... 🙏🏻🙏🏻🙏🏻

@MicroChemsExperiments

3 жыл бұрын

We already worked on Sugar percent but due to some unavoidable issues we are unable to complete the task. We will finish it as early as we can. Thanks for being with us.

Excellent explaination

@MicroChemsExperiments

2 жыл бұрын

Thanks

Thank you.

@MicroChemsExperiments

10 ай бұрын

You are welcome

Zero error practical....❤️❤️

@MicroChemsExperiments

3 жыл бұрын

Thank you. Stay with us.

Best channel

@MicroChemsExperiments

2 жыл бұрын

Thanks

Thanks for your wonderful video and I wonder what the concentration of the NaOH that using to titration HCl 0,5N?

@MicroChemsExperiments

Жыл бұрын

0.5N NaOH solution

Very helpful video,,,

@MicroChemsExperiments

10 ай бұрын

Thanks

THANKS YOUR TUTOR! HOW DETERMINE THE CONCENTRATION OF FLUORIDE AND CHLORIDE IONS FOUND IN GROUNDWATER ARGENTOMETRICALLY?

@MicroChemsExperiments

Жыл бұрын

Noted the parameter. We will make video soon

Thank you

@MicroChemsExperiments

2 жыл бұрын

You are welcome

Good work and great video. Can you do titre test for oil also?

@MicroChemsExperiments

2 жыл бұрын

We will try. Stay with us.

Thanks 👍❤️

@MicroChemsExperiments

2 жыл бұрын

You are welcome

Thanks mam..... For useful💐

@MicroChemsExperiments

2 жыл бұрын

Thanks

It's a good video with very few analytical errors

@MicroChemsExperiments

3 жыл бұрын

Thank you so much. We will try to avoid errors in our next videos.

Thank you so much

@MicroChemsExperiments

Жыл бұрын

You're welcome!

@brandonhu285

Жыл бұрын

@@MicroChemsExperiments Your video contributed to me passing AP Chem with a “ college like” teacher 🫶🏼

Can i use any other solvent like IPA or Methanol instead of Ethanol

Thank you for this vid. I just want to know, how do you handle these chemicals as waste after the analysis?

@MicroChemsExperiments

Жыл бұрын

We take help from third party company who handle these waste chemicals to make it discardable to environment

I'm learning a lot from your videos. More grease to your elbow

@MicroChemsExperiments

Жыл бұрын

Thank you.

Can please explain final calculation part of saponifications because my calculations is different when i done with same values hiw it's 187.88

Super tq

@MicroChemsExperiments

3 жыл бұрын

Thank you

Why do we still have to heat the blank if there's no sample to dissolve

Hello. Thanks for the video. I just wanted to ask the importance of making a blank. And also, can distilled water together with the ethanolic potassium hydroxide solution be used as a blank?

@MicroChemsExperiments

2 жыл бұрын

You must have to prepare blank as shown in this video

@billsmathers7787

Жыл бұрын

The reason for the blank is to account for any water present in the KOH.

@priscillayabani6017

Жыл бұрын

@@billsmathers7787 Thanks

thanks for your awesome tutorial. But, How do I test the fats of snack items like chanachur/chips? Waiting for this tutorials....

@MicroChemsExperiments

3 жыл бұрын

We already uploaded. video link for fat determination from chanachur: kzread.info/dash/bejne/ZGilubiFnZrOmNo.html thank you

@Biplob_BD.Officials

3 жыл бұрын

Thanks

@MicroChemsExperiments

3 жыл бұрын

@@Biplob_BD.Officials Welcome

I want to saponified xanthophyll esters .May I use isopropanol instead of absolute alcohol?

@MicroChemsExperiments

Жыл бұрын

No. You should use ethanol

Great and helpful video..👍😊 Can you make a video on FFA% or acid value of oil?

@MicroChemsExperiments

2 жыл бұрын

Already made: kzread.info/dash/bejne/hpWCk6WinajXlrw.html

Good video! I got Dhatelo Seed Oil from Nepal. However, I am not able to find the SAP value. How can I use it for making soap without SAP value? Thanks.

@MicroChemsExperiments

2 жыл бұрын

Please take help from an expert of soap manufacturing company.

How are you video very helpful for us. Can you make a video of P anisidine value. ( oxidation value)

@MicroChemsExperiments

Жыл бұрын

Noted

When refluxing or heating the KOH and fat...the KOH disappears leaving something like a whitish powder. I then need to refill again with KOH. Why does this happen?

@MicroChemsExperiments

Жыл бұрын

Not sure about that. Never faced this kind of problem

👌👌

@MicroChemsExperiments

2 жыл бұрын

😊😊

U r good btw

@MicroChemsExperiments

3 жыл бұрын

Thank you so much.

Great video, can you explain what is function of reflux 1,67g KOH in 200mL absolute ethanol? Can we only use 6g KOH in 150mL absolute ethanol for preparing 4% ethanolic KOH? Thanks..

@MicroChemsExperiments

2 жыл бұрын

Reflux of ethanol with KOH followed by distillation is normally done to make the ethanol more active for this test. But you can just dissolve 6g in 150ml.

@muhammadrizkyfauzi5003

2 жыл бұрын

Ok thanks a lot..

@hafizishfaq8319

Жыл бұрын

Great video plz make a video .how to check erucic acid in canola oil.

Its my dream to use those gadgets of yours

May I know why we need to dissolve KOH for alcoholic KOH preparation in cold water?

@MicroChemsExperiments

2 жыл бұрын

We need to dissolve KOH in cold environment to prevent the vigorous reaction of KOH with ethanol which may lead to the loss of properties of KOH solution.

in the standard formula for SV, it uses the molecular weight of KOH (56.1g/mol), but in this method, it applies 28. 05? my question is what this number represents?

@MicroChemsExperiments

3 жыл бұрын

Mike Tyson, which standard formula are you talking about. In this test, we followed the AOAC 920.160 official method strictly. In AOAC 920.160 method, 28.05 is used in the equation, so we did.

@TridibSarma

2 жыл бұрын

@@MicroChemsExperiments This is because HCl concentration is 0.5 N. Basically the equation involved HCl concentration too. When it is 0.5 N Molecular wt of KOH is 56.1 which will eventually lead to the value of 28.05.

@MicroChemsExperiments

2 жыл бұрын

@@TridibSarma Thank you so much for the clear explanation.

@user-vs5xc9hd1i

2 жыл бұрын

@@TridibSarmaI don't understand calculations explain please.

@harshitakaushik9058

Жыл бұрын

56.1 x0.5N = 28.05

Ma’am .. as we are always taking 0.5 N HCl for saponification..then why we are getting different range of values for blank .Is there any range for blank value while using 0.5 N HCl .. pls reply

@MicroChemsExperiments

3 жыл бұрын

Sample flask contains sample, thats why during saponification, the concentration of KOH in sample is decreased, thus HCl is needed more in compared with blank.

@MicroChemsExperiments

3 жыл бұрын

Please search on google. You will get idea in details. Thank you.

Mam sample weight is 5gms, but u have concluded that xx mg ofKOH is required to saponify 1gm of fat or oil

❤️

@MicroChemsExperiments

3 жыл бұрын

❤❤

I would like to use this in a paper. What journal can I cite?

@MicroChemsExperiments

3 жыл бұрын

This procedure is not taken from any journal. It is collected from official method of AOAC 920.160. Please cite the AOAC book

Please what is the value 28.05 used in the calculation

what is the purpose aluminum foil for preparing ethanolic?

@MicroChemsExperiments

3 жыл бұрын

Samrawit Alebel, Thank you for your commemts. Aluminum foil acts as a catalyst here and helps to carry out the reaction of KOH with ethanol faster.

Hello! Is it okay to use 95% ethanol instead of 99% in saponification? Thank you

@MicroChemsExperiments

Жыл бұрын

Yes. You can use

Can we use potassium hydroxide flakes instead of pallets

@MicroChemsExperiments

2 жыл бұрын

No. You have to use KOH pallets.

Can you give me the "Determination of Oil Analysis" manual if I need it?

@MicroChemsExperiments

2 жыл бұрын

We do not provide any document

Kindly post sediment test video in Soyabean DOC

@MicroChemsExperiments

3 жыл бұрын

Anil Soni, Noted. We will work on your topic very soon. Stay with us.

👍🏻

@MicroChemsExperiments

2 жыл бұрын

Thanks

Please make a video SAP value on solids also if possible

@MicroChemsExperiments

11 ай бұрын

Okay.

Love from Pakistan

@MicroChemsExperiments

3 жыл бұрын

Thank you so much. Stay with us. Help us to grow.

What should be the temperature for reflux in saponification of coconut oil ???

@MicroChemsExperiments

2 жыл бұрын

Same temperature

Good work, keep it up. But how do you arrive at 4.1ml of HCl? I want to know its percentage purity and density and how you arrived at 4.1ml and must it be 0.5N? Can't 0.1N be used. Thanks as you reply

@MicroChemsExperiments

3 жыл бұрын

0.83ml HCl should be added to make 0.1N HCl solution and 4.1ml is added to make 0.5N HCl solution and make the final volume 100ml. Please watch our video for the preparation of 0.1N HCl. Video link: kzread.info/dash/bejne/noyuo6Rxp9KnlJs.html We used 37% HCl (fuming) of molecular weight: 36.46.

Is the SV of Oil (in the form of triglycerides) less than the SV of the same oil after hydrolysis (free fatty acids after reaction - splitting)?

@ashishshrivastv8375

11 ай бұрын

Yes, because molecular weight decreases after hydrolysis & as the mol wt decreases SV in increase,so definitely SV of split fatty acid will be more than oil before hydrolysis.

how do you say 187.88 mg of KOH required to saponify 1g of fat? you have taken 5 g of sample.

@MicroChemsExperiments

2 жыл бұрын

I divided the equation by the sample weight (5g) to convert the result for 1g of sample. Please take another look at the calculation.

1.How to find SAP value of solid state sample like alovera ? 2. Will the SAP value be same if I use sodium hydroxide? 3.Difference between KOH and NAOH? Please do reply

@MicroChemsExperiments

Жыл бұрын

1. No idea about other sample but you can try this method 2. KOH use is mandatory as said by Reference method. 3. Please take help from other source

@rakeshbh4185

Жыл бұрын

@@MicroChemsExperiments thank you for help

@rakeshbh4185

11 ай бұрын

In formula how did this 28.05 came

If i got 148.5 as a sap value .. Which oil it may be mam ?

@MicroChemsExperiments

2 жыл бұрын

It is may be the oil extracted from almond butter.

please do " determination of total fat matter" in soap..

@MicroChemsExperiments

3 жыл бұрын

We are already working on the parameter. Coming soon. Stay with us.

Some websites are showing this formula of saponificatio = 56.1×(A-B)×N÷W and some showing 28.05 ×(A-B)÷W I am confused, which is correct , what is difference.? Please reply

@MicroChemsExperiments

3 жыл бұрын

Both calculations are same. You will get the same result. Second equation, you mentioned, is simplified form of calculation. First mentioned calculation is the original calculation formula. You can use both.

@aone9548

3 жыл бұрын

@@MicroChemsExperiments thanks for reply

@aone9548

3 жыл бұрын

@@MicroChemsExperiments what is normality of alcohlic KOH solution you have prepared

@MicroChemsExperiments

3 жыл бұрын

@@aone9548 Please watch the video again.

what is the 28.05 in the equation? can u please clear my doubt on 15.00 time

@MicroChemsExperiments

2 жыл бұрын

It is a calculation factor

@ashishshrivastv8375

11 ай бұрын

56.1(mol wt of KOH) *0.5( normality of HCl) = 28.05

hello please share the relationship between sap no and saponifiable matter

@MicroChemsExperiments

Жыл бұрын

Noted

That test can we use Iso Propyl Alcohol

@MicroChemsExperiments

2 жыл бұрын

No. You have to use ethanol

How to prepare 05 N KOH as in how much quatity of KOH is added?

@shraddhapiligaonkar3639

Жыл бұрын

I m asking about ethanol

@MicroChemsExperiments

Жыл бұрын

0.5N KOH is not needed for this test

Ma'am I'm looking process of determination of hydroxyl value of oil any videos or documents

@MicroChemsExperiments

2 жыл бұрын

Noted.

In the equation you put 28.05 and on calculating its become 28.5.

From the calculation aspect, where is 28.05 gotten from???

@16-sahildube31

4 ай бұрын

It is a half of KOH molecular weight 56.11%2 = 28.05

@MicroChemsExperiments

4 ай бұрын

Correct

Oil me insoluble impurities ka video banao

@MicroChemsExperiments

2 жыл бұрын

Noted.

For the calculation why we don't include the normally 0.5 N of the Hcl.

@MicroChemsExperiments

3 жыл бұрын

@Goutam Gupta, this test method and calculation are strictly followed the AOAC 920.160 official method. AOAC given the calculation. The reason behind it is: Sample and blank are Titrated with the same concentration of HCl (0.5N), thats why 0.5 become minimized from each side of sample and blank.

@goutamgupta8816

3 жыл бұрын

@@MicroChemsExperiments thanks for clearing doubt. This channel is really very helpful for we student.keep on ❤️

@MicroChemsExperiments

3 жыл бұрын

@@goutamgupta8816 thanks for being with us.

@ashishshrivastv8375

11 ай бұрын

SV= 56.1*N*(B-S)/w Put Normality -: 0.5N in given equation then you will get SV = 28.05*(B-S)/w

make a video on tomato paste analysis and calculation

@MicroChemsExperiments

Жыл бұрын

Please mention the test parameter of tomato paste

@hassanraza-ng5xh

Жыл бұрын

@@MicroChemsExperiments total energy acidity salt sugar fats protine concentration coler measurement viscosity

@hassanraza-ng5xh

Жыл бұрын

reducing sugar biological analysisi shelf life totsl solids

Why calculate saponification =( (v blank - v sample)× N hcl × 56.11)/sample weight I don't know difference calculate in video?

@MicroChemsExperiments

2 жыл бұрын

The calculation shown in this video is collected from AOAC method. So don't be confused.

@ashishshrivastv8375

11 ай бұрын

Because we are titrating residual KOH by HCl

Determination of Saponification Value of Oil or Fat Sample_A Complete Procedure (AOAC 920.160)

@MicroChemsExperiments

Жыл бұрын

You copied the title. What did you mean?

Same way please upload unsaponification matter which is USM

@MicroChemsExperiments

3 жыл бұрын

NUTHAKKI RAJESH, Okay we will upload USM soon. Please stay with us.

@MicroChemsExperiments

3 жыл бұрын

NUTHAKKI RAJESH, we uploaded the video for Unsaponifiable Matter. Please check it out. Link: kzread.info/dash/bejne/eYCNj6uSm87cZJM.html

Why that 28.05 in the calculation part?

@MicroChemsExperiments

2 жыл бұрын

Calculate Factor given by AOAC

All set but in my calculation answer is 190.90 according to your formula nd what is this 28.05 in formula?

@MicroChemsExperiments

2 жыл бұрын

Calculation factor

@bilalmughal901

2 жыл бұрын

@@MicroChemsExperiments how u make this factor?

@fabihajahan5798

Жыл бұрын

28.05 is the multiplication of (concentration of HCl and the Molecular weight of KOH)

@bilalmughal901

Жыл бұрын

@@fabihajahan5798 okay gud

Please perform the unsonparable matter in oil

@MicroChemsExperiments

3 жыл бұрын

Phool Khoshboo, Noted. We will work on it. Be with us.

@MicroChemsExperiments

3 жыл бұрын

Phool Khoshboo, we uploaded the video for Unsaponifiable Matter. Please check it out. Link: kzread.info/dash/bejne/eYCNj6uSm87cZJM.html

Must And Should Use pipette Not Use Measuring cylinder

@MicroChemsExperiments

4 ай бұрын

Here you don't need accuracy at that much. You can also use measuring cylinder. But using pipette is best practice always

Bellier Turbidity Temperature Test (BTTT) oil test

@MicroChemsExperiments

9 ай бұрын

Noted

Which oil you have used

@MicroChemsExperiments

2 жыл бұрын

Rice Bran Oil

Where does the 28.05 came from? Thank you

@MicroChemsExperiments

3 жыл бұрын

Its a calculation factor given by AOAC official method.

@allanjaymartirez

3 жыл бұрын

@@MicroChemsExperiments it is also 56.11 g/mol (MW of KOH) divided by 2.

Refilling burrette may lead to analytical errors... Should have refilled before starting the titration

@MicroChemsExperiments

3 жыл бұрын

Martin Okyere, Thanks for your observations. You are right about error. We should use 100ml burette to avoid refilling. We have tried to refill it fast to avoid the error as we dont have 100ml burette.

FSSAI revised manual say preparation of Alcoholic KOH different method

@MicroChemsExperiments

2 жыл бұрын

Ok. We will check.

28.05 was written in the first line but in the next line 28.5

@tarunkumar8841

3 жыл бұрын

In the calculation part

@MicroChemsExperiments

3 жыл бұрын

@@tarunkumar8841 Sorry, it was a mistake. 28.05 is the correct factor. So 28.05 should be written in second line also. But we wrote 28.5 by mistake.

how to prepare ethanolic potassium hydroxide solution in procedure

@MicroChemsExperiments

3 жыл бұрын

Preparation of ethanolic KOH is already given in this video. Please follow the step-1.

@amanueldaniel5342

3 жыл бұрын

@@MicroChemsExperiments IS there any optional to prepare without reflux condenser

@MicroChemsExperiments

3 жыл бұрын

@@amanueldaniel5342 You can prepare it without reflux but that can result in some error in the final result. The result will not be exact but can be considerable.

@amanueldaniel5342

3 жыл бұрын

@@MicroChemsExperiments how? please show in procedure

@MicroChemsExperiments

3 жыл бұрын

@@amanueldaniel5342 Just dissolve KOH pellets in absolute ethanol and skip the distillation step. Take help from this saponification video and just skip the distillation part and disoolve KOH in ethanol

Pungency test method please

@MicroChemsExperiments

2 жыл бұрын

Noted

hello sir oil and fat Ka FRA ki full Analysis

@MicroChemsExperiments

3 жыл бұрын

What did you mean by FRA?

What is initial value Vs??

@MicroChemsExperiments

2 жыл бұрын

Vs is the volume of 0.5N HCl which was needed during the titration of saponified sample.

How to make 0.5N hcl solution?

@MicroChemsExperiments

3 жыл бұрын

You can prepare 0.5N HCl solution by followng our video: kzread.info/dash/bejne/noyuo6Rxp9KnlJs.html Just multiply the amount of HCl, needed to prepare 0.1N HCl, by 5. Then follow the whole procedure to standardize the solution.

@factxfact5549

3 жыл бұрын

@@MicroChemsExperiments Hi do you have any idea which tests are important for oil testing according to fssai? I mean on what parameters does fssai check the quality of oil, say mustard oil?

@MicroChemsExperiments

3 жыл бұрын

@@factxfact5549 To test an oil quality, you must go for the following tests: Peroxide value, iodine value, Free Fatty Acid and Acid Value, Rencidity, Saponification value, Unsaponifiable matter, RM value, Polensky Value, Moisture, Refrective Index, Density etc.

@factxfact5549

3 жыл бұрын

@@MicroChemsExperiments every test we have to do if we are manufacturing mustard oil?😳 There are more than 20 tests.

@MicroChemsExperiments

3 жыл бұрын

@@factxfact5549 Yes. You have to do all of those tests.

Where is the 0.5 N in formula ???

@MicroChemsExperiments

2 жыл бұрын

Not needed that value

hello sir oil and fat Ka moisture content

@MicroChemsExperiments

3 жыл бұрын

Already uploaded. Link: kzread.info/dash/bejne/dqiHqpqnYc3IqbA.html

Where come from 28.05

@MicroChemsExperiments

3 жыл бұрын

It is the calculation factor given by AOAC method of saponification value determonation.

@ashishshrivastv8375

11 ай бұрын

Its nothing bus 56.1*0.5

Why say saponification of blank when there is no fat in blank...

@MicroChemsExperiments

3 жыл бұрын

We call it unofficially to make it easy to understand by all including unskilled lab analysts.

Why that 28.05 in calculation

@MicroChemsExperiments

2 жыл бұрын

Calculation factor

This is link of my channel

@MicroChemsExperiments

Жыл бұрын

What do you mean?

De arrr

@mousinalom2007

10 ай бұрын

Dee arrrr

@MicroChemsExperiments

10 ай бұрын

Please write in English

Please don't add background music

@MicroChemsExperiments

2 жыл бұрын

Thanks for you suggestion

Good video, but why does the narrator always talk with a fake accent? Removes the fun of watching the informative video

@MicroChemsExperiments

2 жыл бұрын

We have the limitation of talking and we are very sorry about that. In future we will develop our accent.