Capillary Electrophoresis (Part 6): Reversed Electroosmotic Flow
This video is Part 6 of 6 for the e-Lectures series of NUS Chemistry CM3292 experiment titled "Capillary Electrophoresis (CE) of Inorganic Anions".
Contents:
Reversed electroosmotic flow (EOF)
EOF modifiers
The migration rate of cations, anions and neutral species
By the end of this e-lecture, students will be able to:
1. Discuss how to modify the experimental design of CE in order to reverse the EOF.
2. Explain the migration rate and migration order of cations, anions, and neutral species, under the condition of Reversed EOF.
Thanks for watching :)
Presenter: Dr Francis Chong
Producer: Dr Francis Chong
Artworks: Madebyelvis
Пікірлер: 45
it's called a perfection ... i am a college student from sudan .. thx alot
I'm a pharmacy student from Greece struggling with Pharmacy analysis class. Thank you very much for making it easier for me to understand. Great video very helpful!!
i am a college student from Indonesia, and was really hard to understand my lecturer explanation about this, but by looking to ur nice videos, i really understand to this part now. Thank you, sir! really confident to face the exam tomorrow! Thank you once again, Sir Francis! love
@FrancisChongYY
5 жыл бұрын
@Febriyanti Siahaan, thank you for watching the video. Glad that the video created was helpful :)
THANK YOU so much for this video series, especially for explaining the reversed flow and how the electroosmotic flow modifiers work, your presentation explained it better than a book on the subject of capillary electrophoresis I spent a couple of hours reading.
thank you ssoooooo much for these episodes
Amazingly simplified explanation , keep up , I am waiting for more video , this is how the explanation should be ,this indicates that you really master what you explain , God bless you .
I was so confused and didn't get this at at my lectures but these videos has really helped me understand. Thank you so much!
@FrancisChongYY
4 жыл бұрын
Hi @Rebecka Eriksson, thanks for watching. Glad that the videos help :)
perfect!!!
Superb presentation....well explained...
Very helpful , thank you 🤟
Thank you very much, Sir. I really appreciate your videos pretty much. I am looking forward your future videos, sincerely.
@FrancisChongYY
4 жыл бұрын
Hi @juan tang, thanks for watching. Glad you like them!
Thank you 😍
Hey! Im a pharmacy student from Thailand. I just want to say thank you so much for all video about CE. It really help me to understand about my senior project that I will get in the next semester 🙏🏻🙏🏻 thx u sir! 😀😀
@FrancisChongYY
4 жыл бұрын
Hey! @Supasak Boonprachern, you are welcome :)
Super sir..💟
thank you so much for this lecture series! I was wondering if you could make a lecture series on mass spectrometry? I love your videos and they're helping me so much!
That's called perfection 👍 Really great explanation
@FrancisChongYY
3 жыл бұрын
Thanks a lot 😊
Tq Dr Francis..your explanations are very clear....u help me a lot!!
@FrancisChongYY
4 жыл бұрын
@Nur Idayu Mat Ghani, thanks for watching. Glad that the video helps :)
Great job on this series. I've watched them all, and learned a lot. For this episode, I guessed that perhaps lowering pH would reverse the flow. What would lowering the pH do, would it stop the flow?
Excellent and perfect, thank you very much 💖✨
@FrancisChongYY
3 жыл бұрын
Many thanks
Excellent and perfect ,thank you very much ✨💖
@FrancisChongYY
3 жыл бұрын
You are most welcome. Thank you so much for your compliment.
thanks man i loved your videos they are great! (student biomedical laboratory)
@FrancisChongYY
4 жыл бұрын
Thank you @yasser s :)
Hi, Thanks for your video but I have a question regarding the voltage applied. What would happen if we apply + 30kV, positive voltage, to the reversed electrophoresis at 4:20 ? Will it reverse the EOF toward the cathode instead the anode even with the CTAB? Thanks
Very nice and easy explanation
@FrancisChongYY
3 жыл бұрын
Hi @Soodi Khan, thank you very much for your kind words. Thanks for watching.
Thank you for your video. 👍 I have a question. In lecture 2, silica surface charge can change by pH of buffer solution. So if i change pH of buffer 7.7 to 3, EOF could be reversed?
I like your explanation
@FrancisChongYY
5 жыл бұрын
Thank you, @Socheata Heng. Glad that you like it :)
Sir why here in Reveere osmotic flow neutral sps remain unaffected?
Thankyou so much sir for all your video on CE. In my lab, we have agilent 7100 CE machine. I want to learn how to operate the whole system. Can you please make the video. It will really help me.
@FrancisChongYY
4 жыл бұрын
Hi @Ruchi Dagar, thanks for watching. For a quick start, you may refer to this video here created by Robin Stoodley from UBC: kzread.info/dash/bejne/Y2RmybqzcbmwXZs.html For more details on how to operate the machine, we usually refer to the user manual of the instrument: www.agilent.com/cs/library/usermanuals/public/G7100-90000_CE_System_User_ebook.pdf Alternatively, you may contact Agilent's engineers too.
Nice bro
@FrancisChongYY
5 жыл бұрын
Thanks bro
The direction of flow seems reverse! an I wrong!? It should be toward negative not positive!
@Tranggng
4 жыл бұрын
This is another type of CE, which it reverses the migration of the ions. If you watch his other videos, it's always towards the negative.
@FrancisChongYY
4 жыл бұрын
Hi @Trang Nguyen, thanks for watching, and thank you very much for helping to answer @Hojjat Rostami's question. Yes, you are right :)
@FrancisChongYY
4 жыл бұрын
Hi @Hojjat Rostami, thanks for watching. In this video, reversed electroosmotic flow (EOF) was discussed. Under reversed EOF conditions, the direction of EOF is toward the anode, which is more favorable when analyzing anions as the anions will be eluted out of the capillary first, as compared to the cations and the neutral species. In the case you mentioned, I believe you were referring to the normal EOF conditions. Please kindly check out the Part 2 of my e-lecture series on Capillary Electrophoresis for more details: kzread.info/dash/bejne/ZYxhvLWddpzOh8o.html Thank you. Hope this helps clarify :)