Bial's Test for distinguishing Pentose vs Hexose

Ғылым және технология

Bial's Reagent + Boil (be careful of vapours)
Brown colour change = Hexose
Olive-Green or Bluish-Green colour change = Pentose
Negative Control = Water
Positive Control = Glucose (Hexose)
Positive Control = Xylose (Pentose)
Alphabetical list of Samples:
Arabinose, Fructose, Galactose, Glucose, Xylose

Пікірлер: 10

  • @angellyzlagang1348
    @angellyzlagang13482 жыл бұрын

    You should indicate the name of the test tube next time though

  • @ProfessorDrewCollop

    @ProfessorDrewCollop

    2 жыл бұрын

    Thanks for the feedback. The purpose of this video for viewers to determine what each of the test tubes contain. If I wrote the name on the test tube, it would defeat the whole purpose of the video. I just recorded new version of this video and plan to release it in the coming weeks. The new one will have audio. This one does not, as I did not know how to do that when I started creating. I have learned a lot in the past year.

  • @sergiodiaz860
    @sergiodiaz8603 жыл бұрын

    what is the name of #5 to #12?

  • @ProfessorDrewCollop

    @ProfessorDrewCollop

    3 жыл бұрын

    I cannot tell you which is which as this video is for a lab simulation. The goal of the video is for the students to determine the identity of each. I will provide a list (sorted alphabetically) of the possibilities in the description.

  • @qingyuli5460
    @qingyuli54603 жыл бұрын

    Is there maltose there or no?

  • @ProfessorDrewCollop

    @ProfessorDrewCollop

    3 жыл бұрын

    Maltose is a Disaccharide so it was eliminated in the previous reaction (Barfoed's Test). Only Monosaccharides were assessed using Bial's Test.

  • @qingyuli5460

    @qingyuli5460

    3 жыл бұрын

    @@ProfessorDrewCollop thank youu😅

  • @nourkauteich141
    @nourkauteich1412 жыл бұрын

    why pentoses show bluish colour while hexoses do not?

  • @ProfessorDrewCollop

    @ProfessorDrewCollop

    2 жыл бұрын

    Thanks for the question Nour. Bial's reagent contains orcinol (3,5-dihydroxytoluene), hydrochloric acid and ferric chloride. The acid & heat breaks the pentose ring to create furfural. The furfural will then react with orcinol and the iron ion to form a blue precipitate. Hexose rings will also be broken by the acid & heat but creates hydroxymethyl-furfural. This also reacts, but because the chemistry is different it does not create a blue precipitate. Hope this helps.

  • @nourkauteich141

    @nourkauteich141

    2 жыл бұрын

    @@ProfessorDrewCollop thank you Professor ❤!

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