Bial's Test for distinguishing Pentose vs Hexose
Ғылым және технология
Bial's Reagent + Boil (be careful of vapours)
Brown colour change = Hexose
Olive-Green or Bluish-Green colour change = Pentose
Negative Control = Water
Positive Control = Glucose (Hexose)
Positive Control = Xylose (Pentose)
Alphabetical list of Samples:
Arabinose, Fructose, Galactose, Glucose, Xylose
Пікірлер: 10
You should indicate the name of the test tube next time though
@ProfessorDrewCollop
2 жыл бұрын
Thanks for the feedback. The purpose of this video for viewers to determine what each of the test tubes contain. If I wrote the name on the test tube, it would defeat the whole purpose of the video. I just recorded new version of this video and plan to release it in the coming weeks. The new one will have audio. This one does not, as I did not know how to do that when I started creating. I have learned a lot in the past year.
what is the name of #5 to #12?
@ProfessorDrewCollop
3 жыл бұрын
I cannot tell you which is which as this video is for a lab simulation. The goal of the video is for the students to determine the identity of each. I will provide a list (sorted alphabetically) of the possibilities in the description.
Is there maltose there or no?
@ProfessorDrewCollop
3 жыл бұрын
Maltose is a Disaccharide so it was eliminated in the previous reaction (Barfoed's Test). Only Monosaccharides were assessed using Bial's Test.
@qingyuli5460
3 жыл бұрын
@@ProfessorDrewCollop thank youu😅
why pentoses show bluish colour while hexoses do not?
@ProfessorDrewCollop
2 жыл бұрын
Thanks for the question Nour. Bial's reagent contains orcinol (3,5-dihydroxytoluene), hydrochloric acid and ferric chloride. The acid & heat breaks the pentose ring to create furfural. The furfural will then react with orcinol and the iron ion to form a blue precipitate. Hexose rings will also be broken by the acid & heat but creates hydroxymethyl-furfural. This also reacts, but because the chemistry is different it does not create a blue precipitate. Hope this helps.
@nourkauteich141
2 жыл бұрын
@@ProfessorDrewCollop thank you Professor ❤!