Principle: Reducing Sugars under hot alkaline condition undergoes tautomerization into enediol form. Enediol are strong reducing agents and convertscupric ions of the Benedict'ssolution into cuprous ions. The cuprous hydroxide formed during the process of heating is converted to cuprous oxide as red precipiate.

@DenimDeni

2 жыл бұрын

This is very big sentence

@itsmuaz749

Жыл бұрын

What will happen if we dont heat it? Will it still change colour

@04.d0lly

Жыл бұрын

Thank you so much....

@lolsuprise5094

Жыл бұрын

@@itsmuaz749 nope

@mubasharali8064

Жыл бұрын

p

Thank you for your effort. Please keep uploading videos of more biochemistry test. Thanks

Very helpfull ,plz upload more practicals of biochemistry

Best video...make more video of test sir best

Thanku u for making videosa on practicals such a useful video 🙏🙏thanku so much great work

Sweet voice and good experiment perform

Very good.. Keep it up

Clean sweeep... Covered all points.. It seems... Your student is sincere, obedient than my nouty students..

@Rajesh_Jambhulkar

5 жыл бұрын

😀

@Anush_Sivakumar

3 жыл бұрын

lmfao teacher

@Lilygirl6085

3 жыл бұрын

Lol

@MadridTV12

11 ай бұрын

Nouty 😂😂😂😂😂Rip english 😢😢Rip Such fool teachers 😢😢😢

@pharmatricksforexams5967

11 ай бұрын

@@MadridTV12 hi... Its me... Badri nath... I wrote sweeep also mistake.... Not observed?

Beautiful result...... Thx sir...!

Nice explanation...! You Are The Best ! ❤❤❤ ❤❤❤❤ ❤❤❤❤❤❤ ❤❤❤❤❤❤❤ ❤❤❤❤❤❤❤❤ ❤❤❤❤❤❤❤❤❤❤

Please upload more videos on practicals

Awesome sir 👏 🙌 👌

Outstanding explanation

Good Informative

Thanks Archana ma'am 💓💓💓💓💓💓

Nice video 😊

Very helpful sir by the way I m not MBBS student, I am From Paramedical (MLT) field & sometimes I need to watch your lecture sir & pls sir upload a more Practical video such as it is Benedict's test Thanks sir

@insightfulmotivebyzehra2309

Жыл бұрын

In which clg??

@nitinkumargupta635

Жыл бұрын

@@insightfulmotivebyzehra2309 Sanskriti University

@thesleema6975

Жыл бұрын

Iam also a MLT students

@insightfulmotivebyzehra2309

Жыл бұрын

@@thesleema6975 kis University se ho?

@faizan7343

Жыл бұрын

Tatty university

Thanks dikhsha 💓💓💓

seriouly!!! waterbath is used for heating so the heat spreads equally its so dangerous to shake the tube like that it can also spill

Does the solution turn green *to* brick red *after* cooling? I feel green ppt was due to less concentration of the reducing sugars in the solution, and brick red due to higher concentration. Right? Well explained👍

@gunachandhiran3345

2 жыл бұрын

Depending upon theamount of reducing sugar in the urine the color will deffer such as green(+),yellow(++),orange(+++) and prik red(++++)

Thank u for your wonderful vedio.nyc explanation.

Sir if I learn nicely all ur vedios can I score well in biochem in proff?

i am going to buy test tubes .

Thank you !

Can you use some kind of catalyst for this method , and deposit that copper on som surface ,or exsample gripping a copper mirror?

thanks sir

Thanks a lot

Useful

Thank you so much sir😊

Home sugar positive test?

Keep it up

Or video upload kijiye sir ji practical k liye

Hi, may I know the benedict solution will reacts faster in less or more concentrated glucose solution?

👏👏👏👏

Good

Name of the sample added?

Why there in need to convert cuprous hydroxide into cuprousoxide form by heating??

Provide other exp too

Milk as a sample le to positive test hoga?

👍🏻👍🏻🙏🏻

What is the name of this test particularly?

Why heated alkaline is used

What will the result be if you tested a tomato and if you tested a cucumber. Please help.

what was the sample used?

@UdayRaj-mz5sf

3 ай бұрын

Urine sample

Sample means I mean kesa sample what we can take in it

How to make sample

It’s a pipette not a droper

👍👍👍

Thanks mam

👌👌👌👌👌👌👌

👌👌👌👌👌👌👌👌👌👌👌👌👌❤️

What is sample

Need Benedict test for making assigment

Mam if while heating the colour doesn't change but after 15 minutes you get red ppt settle at bottom, then will it be reducing sugar

Sir if we have to recognize non redusing sugar and reduisng sugar which are in one solution ,how can we identify them separately?

@Abdullahkill3333r

2 жыл бұрын

you would seperate the sample, and test both separately. as far as I know - there's no way to actually test for non reducing sugars and reducing sugars within one test tube

@aahilzaman6682

Жыл бұрын

If the Benedict’s test gives negative result then we have to slightly heat it using hydrochloric acid and then let it cool down.Then add an alkali like sodium hydrogen carbonate to break down the glycosidic bonds in the non reducing sugar and then carry out the test.

❤❤❤🎉🎉🎉🎉

Sample Kya cheze ha?

If Benedict's test shows blue at the start, dont we already know its non reducing, so why do the things after? Y is hcl used instead of water to break down sugar?

@chosnotes162

2 жыл бұрын

i think it's to determine the concentration

Do you do online teaching sister?

What is the sample mixed of

@Sreekuttyvishnu1999

2 жыл бұрын

Urine

Sugar can be tested in a diabetes patient by। Benedict's test or barford test

when auto analyzer was not there how pathologists measured blood sugar?

@Rajesh_Jambhulkar

4 жыл бұрын

By manual methods on colorimeter

What are these gm%? Is these amounts for glucose?

@Rajesh_Jambhulkar

3 жыл бұрын

gm/dl

@nutritalks9938

3 жыл бұрын

Gm glucose present in 100ml solution?

Any pdf

Why should we add only 8 drops of urine because it is a semi quantitative the thing is what happens if we add more drops of urine or less ?

@sumithrashankar9072

2 жыл бұрын

If u add more .the non- carbohydrate which have a reducing property gives false positive for Benedict test .

Why don't u sue thermostatic controlled water bath

Rajesh koothripali

@imanabass5660

3 жыл бұрын

Oh my I remember him from Big Bang theory

@imanabass5660

3 жыл бұрын

Same

@imanabass5660

3 жыл бұрын

Me too

@shotfox6371

2 жыл бұрын

This is sad

What is gm%?

@Rajesh_Jambhulkar

4 жыл бұрын

Gm/100ml

Does a non-carbohydrate substance be positive in Benedict’s test?

@man-mk2bx

3 жыл бұрын

Nope

@limeffsss

2 жыл бұрын

No, maltose and glucose are types of sugars- carbohydrates. Non-carbohydrate substances will definitely not have these two reducing sugars present. The solution will remain blue (CuSO4- Copper Sulphate).

@sumithrashankar9072

2 жыл бұрын

Yes.Non_ carbohydrate like uric acid,ascorbic acid ,glutathione give false positive for Benedict test

Benedict's reagent react with reducing sugar color get yellow,.why? give the scientific reason easy to understand...

@Rajesh_Jambhulkar

5 ай бұрын

Reducing sugar in hot alkaline medium forms strong reducing agent Enediol. This reduces cupric ions to cuprous form. There is formation of Cu(OH)2 and CuO2 which gives different colors dependent on sugar concentration.

اختبار عملي

Please next time use a water bath

Freshy 😂😂😂

It is not right method.

DILI MAKA CHECK OUT. IF DI MAKA TAN.AWS VIDEO HAHHAAH

Hari bokkala