Antibiotic Sensitivity Test by Disc diffusion method (Kirby Bauer method & Stokes method)

This video demonstrates how to perform a disk diffusion test includes Kirby Bauer and stokes method. This method is used for testing the susceptibility of bacteria and fungus to different antimicrobials.
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Пікірлер: 57

  • @chigbataosaletin1215
    @chigbataosaletin1215Ай бұрын

    This really helps me a lot in carrying out sensitivity test in my lab

  • @TAMSUN14
    @TAMSUN143 жыл бұрын

    Very interesting sir..Also you did very nice performance this work. This like videos very useful for all trainers. Thanks for your support. Bye.

  • @shisampoudel532
    @shisampoudel5322 жыл бұрын

    Thanks for making this video.

  • @aryanhanamshet6823
    @aryanhanamshet68232 жыл бұрын

    Extremely helpful, thank you so much.

  • @relaxingshorts9928
    @relaxingshorts99283 жыл бұрын

    Sir u are simply superb😍

  • @maddelaanjaneyulu4615
    @maddelaanjaneyulu46152 жыл бұрын

    Sir this video helps me a lot thank you

  • @jamaicagamus8967
    @jamaicagamus89673 жыл бұрын

    This really helps me a lot about my individual report, thank you very much 💖

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    @valentinokayden6964

    3 жыл бұрын

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    @zanejimmy6905

    3 жыл бұрын

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    @ryankendrick6350

    3 жыл бұрын

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    @gusomar7213

    3 жыл бұрын

    @Valentino Kayden Yup, I have been watching on Flixzone for months myself :)

  • @Thinker-gd8ck
    @Thinker-gd8ck3 жыл бұрын

    Thanks for sharing!

  • @bashgen4211
    @bashgen42112 жыл бұрын

    The best so far

  • @hddggfdaa
    @hddggfdaa Жыл бұрын

    What will be the distance between two disc in 110 mm Mueller Hinton Agar?

  • @Blevic18
    @Blevic18Ай бұрын

    Which chart did you use as reference please and where can I download it?

  • @divya821
    @divya8212 жыл бұрын

    Sir helpful thanks

  • @shubhadathorat3522
    @shubhadathorat3522 Жыл бұрын

    How can we take reading of 2mm or 2cm fabric by this method

  • @meenageeths9730
    @meenageeths973011 ай бұрын

    Sir helpfull thanks 👍

  • @afnan8610
    @afnan86102 жыл бұрын

    Thanks 👏👏👏👏✨⭐️⭐️

  • @anjua3771
    @anjua3771 Жыл бұрын

    How wil yuh add sample at different concentrations??

  • @salmaallah3703
    @salmaallah370325 күн бұрын

    How many colonies do we need to pick ? Thanks

  • @paballomoono2940
    @paballomoono29403 жыл бұрын

    What's the purpose of comparing the bacterial suspension with the 0.5 McFarland standard?

  • @rejojacobjoseph

    @rejojacobjoseph

    3 жыл бұрын

    www.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380

  • @yasir3719
    @yasir37192 жыл бұрын

    Sir I prepare sample 10mg/ml concentration. For standard I use ofloxacin so I make the standard in same conc as test samples are?

  • @rejojacobjoseph

    @rejojacobjoseph

    2 жыл бұрын

    No need to prepare standard in same concentration.

  • @linussamuel8748
    @linussamuel8748 Жыл бұрын

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  • @benefoebenezer9835
    @benefoebenezer9835 Жыл бұрын

    Wonderful. How do I get the interpretation charts for the antibiotics. Any help will be much appreciated. Thanks

  • @rejojacobjoseph

    @rejojacobjoseph

    Жыл бұрын

    Interpretation chart u will get it from the antibiotic company site / u can enquire with the suppliers.

  • @thuitamwangi5401
    @thuitamwangi54012 жыл бұрын

    👍May God bless you

  • @divya821
    @divya8212 жыл бұрын

    But vollume is very slow kindly increase pls

  • @pink35day5
    @pink35day510 ай бұрын

    Is incubation not required if bacterial suspension is prepared using MH broth, as with glucose broth or peptone water where the suspension is incubated before making a lawn

  • @rejojacobjoseph

    @rejojacobjoseph

    10 ай бұрын

    Incubation is required after inoculation

  • @shubhadathorat3522
    @shubhadathorat3522 Жыл бұрын

    Can this method work for 2cm or 2mm fabric test

  • @mrstevens445
    @mrstevens4452 жыл бұрын

    Thanks for this. I however have a question. I did triplicate, so I find the average before comparing to an interpretation chart? Please help me

  • @rejojacobjoseph

    @rejojacobjoseph

    2 жыл бұрын

    Yes. U can take the average and then compare with interpretation chart. But the difference of the trip. results should be near by. if ur doing sensitivity test with ur own prepared antibiotic then u need to do triplicate otherwise no need.

  • @mrstevens445

    @mrstevens445

    2 жыл бұрын

    @@rejojacobjoseph thank you

  • @davidzhang6076
    @davidzhang60762 жыл бұрын

    Sir i have a question, how big should the antibiotic disc be? Thank you

  • @rejojacobjoseph

    @rejojacobjoseph

    2 жыл бұрын

    6mm plain disc (for research purpose) we r getting at 8mm in size

  • @coffeestarsgalaxy9369
    @coffeestarsgalaxy93692 жыл бұрын

    what culture did you use sir, is it s. aureus and e. coli?

  • @rejojacobjoseph

    @rejojacobjoseph

    2 жыл бұрын

    S.aureus

  • @nyesuraju912
    @nyesuraju9127 ай бұрын

    Tq sir

  • @mdobaid4129
    @mdobaid41292 жыл бұрын

    In stokes method same antibiotics disc we've to use and same bacteria for test and control?

  • @rejojacobjoseph

    @rejojacobjoseph

    2 жыл бұрын

    Same bacteria for test & the control.. e.g: for the test u can use identified Hospital sample and for the control u need to use standard strain (ATCC strains).

  • @sureshalamuri3666
    @sureshalamuri3666 Жыл бұрын

    Well sir

  • @sasikalac4874
    @sasikalac4874Ай бұрын

    Why the bacterial suspension is compared to 0.5 Mc farland standard

  • @rejojacobjoseph

    @rejojacobjoseph

    Ай бұрын

    It helps to ensure that the number of bacteria will be within a given range to standardize microbial testing.

  • @beyzasualp2225
    @beyzasualp22253 жыл бұрын

    Why is there no Turkish translation?

  • @yuvrajkatkar2597
    @yuvrajkatkar25972 жыл бұрын

    👍👍

  • @user-kg5do2vt2p
    @user-kg5do2vt2p7 ай бұрын

    Sorry, can you write the method here?

  • @sasmitadas345
    @sasmitadas345 Жыл бұрын

    Sir is this entire procedure done inside luminar air flow

  • @rejojacobjoseph

    @rejojacobjoseph

    Жыл бұрын

    Yes..All the works must done in the safety cabinet only.

  • @sabhakailash7728
    @sabhakailash77283 жыл бұрын

    👍👏👏👏👏

  • @hddggfdaa
    @hddggfdaa Жыл бұрын

    What is the distance between the disc and the periphery?