Antibiotic Sensitivity Test by Disc diffusion method (Kirby Bauer method & Stokes method)
This video demonstrates how to perform a disk diffusion test includes Kirby Bauer and stokes method. This method is used for testing the susceptibility of bacteria and fungus to different antimicrobials.
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Пікірлер: 57
This really helps me a lot in carrying out sensitivity test in my lab
Very interesting sir..Also you did very nice performance this work. This like videos very useful for all trainers. Thanks for your support. Bye.
Thanks for making this video.
Extremely helpful, thank you so much.
Sir u are simply superb😍
Sir this video helps me a lot thank you
This really helps me a lot about my individual report, thank you very much 💖
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3 жыл бұрын
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3 жыл бұрын
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3 жыл бұрын
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Thanks for sharing!
The best so far
What will be the distance between two disc in 110 mm Mueller Hinton Agar?
Which chart did you use as reference please and where can I download it?
Sir helpful thanks
How can we take reading of 2mm or 2cm fabric by this method
Sir helpfull thanks 👍
Thanks 👏👏👏👏✨⭐️⭐️
How wil yuh add sample at different concentrations??
How many colonies do we need to pick ? Thanks
What's the purpose of comparing the bacterial suspension with the 0.5 McFarland standard?
@rejojacobjoseph
3 жыл бұрын
www.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380
Sir I prepare sample 10mg/ml concentration. For standard I use ofloxacin so I make the standard in same conc as test samples are?
@rejojacobjoseph
2 жыл бұрын
No need to prepare standard in same concentration.
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Wonderful. How do I get the interpretation charts for the antibiotics. Any help will be much appreciated. Thanks
@rejojacobjoseph
Жыл бұрын
Interpretation chart u will get it from the antibiotic company site / u can enquire with the suppliers.
👍May God bless you
But vollume is very slow kindly increase pls
Is incubation not required if bacterial suspension is prepared using MH broth, as with glucose broth or peptone water where the suspension is incubated before making a lawn
@rejojacobjoseph
10 ай бұрын
Incubation is required after inoculation
Can this method work for 2cm or 2mm fabric test
Thanks for this. I however have a question. I did triplicate, so I find the average before comparing to an interpretation chart? Please help me
@rejojacobjoseph
2 жыл бұрын
Yes. U can take the average and then compare with interpretation chart. But the difference of the trip. results should be near by. if ur doing sensitivity test with ur own prepared antibiotic then u need to do triplicate otherwise no need.
@mrstevens445
2 жыл бұрын
@@rejojacobjoseph thank you
Sir i have a question, how big should the antibiotic disc be? Thank you
@rejojacobjoseph
2 жыл бұрын
6mm plain disc (for research purpose) we r getting at 8mm in size
what culture did you use sir, is it s. aureus and e. coli?
@rejojacobjoseph
2 жыл бұрын
S.aureus
Tq sir
In stokes method same antibiotics disc we've to use and same bacteria for test and control?
@rejojacobjoseph
2 жыл бұрын
Same bacteria for test & the control.. e.g: for the test u can use identified Hospital sample and for the control u need to use standard strain (ATCC strains).
Well sir
Why the bacterial suspension is compared to 0.5 Mc farland standard
@rejojacobjoseph
Ай бұрын
It helps to ensure that the number of bacteria will be within a given range to standardize microbial testing.
Why is there no Turkish translation?
👍👍
Sorry, can you write the method here?
Sir is this entire procedure done inside luminar air flow
@rejojacobjoseph
Жыл бұрын
Yes..All the works must done in the safety cabinet only.
👍👏👏👏👏
What is the distance between the disc and the periphery?