I think the representation of -PO4 and -OH group at either end (any one) is wrong (0:57 min). You need to swap the functional groups at one of the two ends to maintain the 5' to 3' orientation correct. But you corrected it afterwards at 1:10 min. The video is very useful by the way. Best wishes!

Learning from Bangladesh.

@animatedbiologywitharpan

Жыл бұрын

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@animatedbiologywitharpan

4 жыл бұрын

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Thank you so much this was super informative

@animatedbiologywitharpan

4 жыл бұрын

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Something to comment, polynucleotide kinase use dNTP labelled in the gamma phosphate, not alpha. This last one is used by polymerase.

Sir how oH is left behind when we remove phosphate ? Nucleotide have 3 phosphate the phasphatase removes all the three phosphates or only one phosphate ?

Can you please make a video on packaging extracts. These videos are great.

@animatedbiologywitharpan

Жыл бұрын

I am unsure about this topic. Could you please elaborate

@_a_celestial9930

Жыл бұрын

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@animatedbiologywitharpan

5 ай бұрын

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Which software do you use for this beautiful representation and diagram

@animatedbiologywitharpan

Жыл бұрын

Only PowerPoint, if you want to learn please email me at arpanparichha1994@gmail.com

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@animatedbiologywitharpan

4 жыл бұрын

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@animatedbiologywitharpan

4 жыл бұрын

debapriya das 9987997041

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@animatedbiologywitharpan

2 жыл бұрын

Please please share my channel link with your friends and help me to reach big audiance

1.40 , Polynucleotide kinase only acts on 5' end , then how it add phosphate in 3' end of PCR products (As you mentioned)🤔🤔🤔🤔🤔🤔🙇🏼‍♂️🙇🏼‍♂️🙇🏼‍♂️🙇🏼‍♂️

I think the PCR product does contain 5' phosphate group and does not require to be phosphorylated after PCR reaction by polynucleotide kinase. Please correct me if I am wrong.

@animatedbiologywitharpan

4 жыл бұрын

No you are correct in your point.... the trick prople use in lab is to put phosphate group also in the 3’ end using pnk so both 3&5’ are phosphate and the vector you have to use you use alkaline phosphatase to cleave 5 prime phosphate and as a result the efficiency increase

@animatedbiologywitharpan

4 жыл бұрын

May be I should have explained it better

@sharduldhole5701

4 жыл бұрын

@@animatedbiologywitharpan Thanks for clarification.

What is exchange reaction by polynucleotide kinase?

@syed-wd8ib

4 жыл бұрын

Can anyone explain it?