This is the channel for the Aging Research and Drug Discovery meeting (www.agingpharma.org). We reserve the right to remove derogatory, personal and/or non-scientific comments.
Stop funding Washington DC and start funding science of scientists and doctors Last 40 years were a waste of trillions 😳😳😳🍳
@superluminal89Ай бұрын
From DIscord: "Obviously only a few slides, but RPE reprogramming data @ 12:55 in a mouse model of macular degeneration, and neuron reprogramming data @ 14:25. IMO the new optomotor acuity data in mice w/ reprogrammed RPE cells look better than in their prior work in the optic nerve, but these are very different diseases/models so such a comparison isn't really appropriate, especially without data from a full paper (I'm just trying to put into context the extent of vision improvement here). Also they used an iodide model of macular degeneration - but presumably in older mice that are 12 months old, which is still quite young - which while is a standard mouse model in eye research I doubt it recapitulates normal aging and/or age-related macular degeneration well. David also presents some early MIB-626 (his company's NMN) human trial data showing potential improvements in a few cardiovascular biomarkers like total cholesterol, non-HDL cholesterol, and also improved weight loss"
@Ab-jk8pdАй бұрын
Breakthrough, Excellent , Doctor's
@SkipYearSixАй бұрын
I can’t spend 20 years in 24/7 pain with chronic disease 😭
@joaquinvitale1646Ай бұрын
Absolutely ❤
@Lily-yb8qdАй бұрын
How can you have a low calorie yet high fat diet?
@Krunch20202 ай бұрын
I’m looking forward to the results from the fisetin study. Or did it fail??
@norahsdinsdale37282 ай бұрын
I have osteoarthritis and needing two hips i want to volunteer for drug trials, is this possible, im healthy, non smoker, can anyone help me
@lucasguazzinilampugnani36572 ай бұрын
Very interesting.. i Think it won't be on the public radar.. in most countries... or every public pension system will collapse.. The ideal for a government should be stay healthy until a defined age and then die.. live longer = bankrupt (I obviously assume it won't be possible to quickly increase retirement age for most of the jobs)
@user-fs8tl7ni1w2 ай бұрын
Google: GlaxoSmithKline and David Sinclair. You’ll come to know more about David. A lot more.
@thingizi642 ай бұрын
You are brilliant! Thank you for your research!
@peterz533 ай бұрын
On VL's comment about not being a ketogenic diet it nevertheless raises ketones and lowers glucose, alot. I've done the official FMD and my own version based on the macro breakdown and in all case my ketones went up to clinical levels (.0.5 mmol) in the 2 to 3 mmol range by day 3 of 5. I take this as just a marker of low insulin. My glucose went down to 70s from high 80s/low 90s. Highly recommended. But do before and after blood work. And when you come out of transition to a good diet and TRE if you can. I did the FMD as two meals/day about 8 hours apart, so a form of TRE combined with FMD.
@fourthrockonearth3 ай бұрын
Os it just me or did she describe a great leap forward to cure blindness...
@peterbedford26103 ай бұрын
In his book, Longo shows how to do a DIY version of the FMD. Very easy. I do it once a year.
@peterz533 ай бұрын
Yes, I designed my own after doing the official version 3 times. Works very well - Using ketone and glucose levels as markers my version gave same results.
@jaym98463 ай бұрын
Very interesting. Never heard much about IL-11 before.
@nictegki3 ай бұрын
Thank you so much! Muchas gracias from Mexico!
@nictegki3 ай бұрын
Muchas gracias, thank you so much
@Fedeniux4 ай бұрын
Very interesting talk. Love biology
@MrStarchild30014 ай бұрын
Vittorio Sebastiano from Turn Biotechnologies presented at the 10th Aging Research and Drug Discovery conference on cellular and tissue rejuvenation through epigenetic reprogramming. The key points from his presentation are: 1. Epigenetic drift, the progressive accumulation of epigenetic errors in the epigenome, is believed to be a fundamental driver of cellular aging and loss of function. Over time, genes that should be off get turned on and vice versa, impacting many hallmarks of aging. However, unlike the genetic code, the epigenome is reversible and can potentially be reset. 2. Inspiration for epigenetic reprogramming comes from the works of John Gurdon and Shinya Yamanaka showing that somatic cells can be reprogrammed into embryonic-like cells through somatic cell nuclear transfer (Gurdon) or induced pluripotent stem cell techniques (Yamanaka). This suggests aged cells could potentially be made more youthful. 3. In 2014, Sebastiano's group proposed using a controlled partial/transient reprogramming approach to change the age of cells without altering cell identity. They published foundational work on this "epigenetic reprogramming of aging" (ERA) in 2020 using a cocktail of 6 factors delivered as mRNAs. Since then, several studies have validated the approach in various tissues (muscle, skin, brain etc.) and organisms (mice, rats, primates). 4. Turn Biotechnologies is working to translate ERA into clinical applications by combining the biological concept, mRNA delivery vehicles, and lipid nanoparticle (LNP) delivery systems. Using mRNA avoids genomic integration risks and allows fine control of factor expression. Certain factors like MECP2 have beneficial effects when pulsed briefly in combination with others, contrary to some misconceptions. 5. In dermatology, ERA shows promising results: - In primary human fibroblasts, it causes transcriptomic resetting with activation of ECM related genes, anti-inflammatory effects etc. Fibroblast identity genes are appropriately modulated. - In human skin biopsies cultured ex vivo, aging biomarkers are favorably altered after ERA treatment. - An in vivo human skin xenograft model was developed to test ERA delivery and efficacy over several weeks. 6. For immunology, ERA is being explored to rejuvenate T cells: - Treated T cells from old donors proliferate robustly in response to tumor cells without any change in cell identity markers. - They express lower levels of exhaustion markers and maintain better anti-tumor cytotoxic function compared to controls. 7. ERA has potential applications in many tissues like muscle, eye, cartilage, blood, and skin, with dermatology and immunology being the initial focus areas. 8. Delivery of mRNA remains a key challenge, especially achieving sufficient penetration. For skin, microneedling/injections are the near-term approach, with topical solutions being developed. Broader interest and investment in mRNA and gene therapies, catalyzed by COVID vaccines, is expected to accelerate progress on novel delivery systems. In conclusion, Sebastiano presented compelling evidence for ERA as a promising approach to partially reverse cellular aging through transient epigenetic reprogramming. By translating ERA into viable clinical therapies, Turn Bio aims to ultimately increase human healthspan. While delivery hurdles remain, the underlying biology appears sound and reproducible across contexts. ERA represents an exciting frontier in the fight against aging and age-related dysfunction.
@snorremortenkjeldsen6737Ай бұрын
Thank you for the great summary :)
@mikeholman42844 ай бұрын
Doing my first round of FMD. Primarily interested in anti cancer. Wondering if alternating the FMD with a vegan KETO would stress cancer cells more than just FMD. Example, month 1, FMD, month 2 Ketogenic diet (5 days).
@peterz534 ай бұрын
Thanks. Very important. As a 69 yo male very encouraging as I exercise daily these last 12 years. Hba1c stays ~ 5% and hsCRP at <= 0.3 mg/L consistently. Will test IL-6 on morning before exercise to confirm it is low.
@Simone-lb8zr4 ай бұрын
The research is absolutely stunning and many people are pending from any news regarding this study. However, I think the difference between targeting "degenerating" or "dead" retina cells in the end of the talk, could have worth a bit more time explanation. Also, do you plan a glaucoma model for the non-human primate test?
@Nahid07official4 ай бұрын
I've read his book lifespan couple weeks ago.. and thereafter I'm looking for his interviews
@jordanong51144 ай бұрын
If he becomes my teacher, I’d be honored.
@georgiaparaskevopoulou74255 ай бұрын
That is amazing!! I will like to hear more...l would like also read the book Lifespan!!😊by David!!
@anngraves69175 ай бұрын
Pure NMN powder from donotage is really good.
@dalevandenheuvel39275 ай бұрын
I would like to know you said this company is going to make NMN Into a drug, how is that going to affect the rest of the people that you are educating on his benefits, to me that would limit it greatly as to who can get it?
@malcolmlklein5 ай бұрын
I wonder if he has looked at senescent cells? Are they reduced as in senolytic therapies?
@jeanneturnpaw36255 ай бұрын
So VERY DISAPPOINTED that you are associated with the WEF. As we all know Klaus Schwab, Gates and the WEF are for depopulation of the world by billions!! Unless these supplements are only for the elites I am very hesitant to believe what is presented. Maybe the average "useless eater", as Klaus calls the general population is not supposed to hear this information or take the supplements!
@charlessavoie23675 ай бұрын
Did I hear him claim to have dead lifted almost 1,000 pounds?? If so can't he hold a stationary Iron Cross on still rings with 100 extra pounds strapped around his waist?
@diqnu5 ай бұрын
Did he say, penguins are mammals?
@DorianGray-pi4ov4 ай бұрын
It's a bit vague. Something like, "mammals can go long periods of fasting, LIKE these emperor penguins." So the way I read it is that; mammals can fast like penguins. Rather than penguins are mammals (which is incorrect of course, they are birds).
@stefanallard30845 ай бұрын
Methionine is better then acuall sam-e for creating sam e and having a anti depressive effect new studies show. Yet many years back aging docs pushed that methionine restriction increase lifespan. Wich makes no sense as methionine is a must for methylation and sam e creation.
@houndofzoltan5 ай бұрын
I want to move to Singapore now.
@houndofzoltan5 ай бұрын
Seems criminal that I can't make an informed decision by myself to use rapamycin and take the risks: the possible rewards seem worth it. The whole "we need rapalogues cuz pharma isn't interesting in rapamycin cuz it can't be patented is infuriating.
@jujjuj76765 ай бұрын
really the one person to ask a question was some girl who wanted to know what to study...great way to waste it...🙄
@TheRestalyn5 ай бұрын
I can tell he is a toxic PI the way he talks about his student
@houndofzoltan5 ай бұрын
She seemed very nervous, but she did a great job. Very interesting talk.
@heatherkelley92535 ай бұрын
I have done the Prolon 5 day fasting mimicking diet and having the prescribed food made it possible to tolerate the diet because extreme hunger wasn't a factor. I believe in Dr. Longo's research.
@13thbiosphere5 ай бұрын
I'm on a Six-Day fasting having about 1,000 calories in six days I drink olive oil +pollen spirulina and a handful of walnuts, 23 hours bed rest per day, I have a very small amount of high dense nutrition
@user-os2gk7gw5q6 ай бұрын
Love this
@Thaythichgiachanh2626 ай бұрын
Thank you very much for your valuable information ♥👍👍
@user-qb9od7pu3c6 ай бұрын
What is the mechanism to revert age by applying four Yamanaka factors?
@user-qb9od7pu3c6 ай бұрын
It's important to know basic underlying mechaminism resetting epigenetic setting cell. This will be found by some genius.
@larsnystrom66986 ай бұрын
A very interesting talk and subject. I'm surprised so few seems to react on it. Only one comment, for example!
@AkkarisFox6 ай бұрын
A price implies a selective process that perpetuates the cycle of disenfranchisement for those who have no substantial income beyond housing and food.
@SirTenenbaum3 ай бұрын
Unless the cost is covered by third-party payers, such as insurers, charities, and governments.
@JacobAFarmer6 ай бұрын
Wow, that is super-exciting research. Great talk.
@maokai1236 ай бұрын
Fantastic. Dr.Coray provides comprehensive evidences to prove this old prediction is true.
@jip34436 ай бұрын
Bus drive surely could have waited one more question, you have David Sinclair on stage. Come on!
@lubnaahmed12996 ай бұрын
❤❤❤
@larsnystrom66986 ай бұрын
This was most interesting! The connection between mitocondria and senescence is new to me, and seems to change things compleatly. The similarity between apoptosis and senescence is intriguing! I wonder what effect PhotoBioModulation would have on senescencent cells. Since that affects the mitocondria. Would it push them into apoptosis, into recovery, or neither?
Пікірлер
Bla bla bla…..
Stop funding Washington DC and start funding science of scientists and doctors Last 40 years were a waste of trillions 😳😳😳🍳
From DIscord: "Obviously only a few slides, but RPE reprogramming data @ 12:55 in a mouse model of macular degeneration, and neuron reprogramming data @ 14:25. IMO the new optomotor acuity data in mice w/ reprogrammed RPE cells look better than in their prior work in the optic nerve, but these are very different diseases/models so such a comparison isn't really appropriate, especially without data from a full paper (I'm just trying to put into context the extent of vision improvement here). Also they used an iodide model of macular degeneration - but presumably in older mice that are 12 months old, which is still quite young - which while is a standard mouse model in eye research I doubt it recapitulates normal aging and/or age-related macular degeneration well. David also presents some early MIB-626 (his company's NMN) human trial data showing potential improvements in a few cardiovascular biomarkers like total cholesterol, non-HDL cholesterol, and also improved weight loss"
Breakthrough, Excellent , Doctor's
I can’t spend 20 years in 24/7 pain with chronic disease 😭
Absolutely ❤
How can you have a low calorie yet high fat diet?
I’m looking forward to the results from the fisetin study. Or did it fail??
I have osteoarthritis and needing two hips i want to volunteer for drug trials, is this possible, im healthy, non smoker, can anyone help me
Very interesting.. i Think it won't be on the public radar.. in most countries... or every public pension system will collapse.. The ideal for a government should be stay healthy until a defined age and then die.. live longer = bankrupt (I obviously assume it won't be possible to quickly increase retirement age for most of the jobs)
Google: GlaxoSmithKline and David Sinclair. You’ll come to know more about David. A lot more.
You are brilliant! Thank you for your research!
On VL's comment about not being a ketogenic diet it nevertheless raises ketones and lowers glucose, alot. I've done the official FMD and my own version based on the macro breakdown and in all case my ketones went up to clinical levels (.0.5 mmol) in the 2 to 3 mmol range by day 3 of 5. I take this as just a marker of low insulin. My glucose went down to 70s from high 80s/low 90s. Highly recommended. But do before and after blood work. And when you come out of transition to a good diet and TRE if you can. I did the FMD as two meals/day about 8 hours apart, so a form of TRE combined with FMD.
Os it just me or did she describe a great leap forward to cure blindness...
In his book, Longo shows how to do a DIY version of the FMD. Very easy. I do it once a year.
Yes, I designed my own after doing the official version 3 times. Works very well - Using ketone and glucose levels as markers my version gave same results.
Very interesting. Never heard much about IL-11 before.
Thank you so much! Muchas gracias from Mexico!
Muchas gracias, thank you so much
Very interesting talk. Love biology
Vittorio Sebastiano from Turn Biotechnologies presented at the 10th Aging Research and Drug Discovery conference on cellular and tissue rejuvenation through epigenetic reprogramming. The key points from his presentation are: 1. Epigenetic drift, the progressive accumulation of epigenetic errors in the epigenome, is believed to be a fundamental driver of cellular aging and loss of function. Over time, genes that should be off get turned on and vice versa, impacting many hallmarks of aging. However, unlike the genetic code, the epigenome is reversible and can potentially be reset. 2. Inspiration for epigenetic reprogramming comes from the works of John Gurdon and Shinya Yamanaka showing that somatic cells can be reprogrammed into embryonic-like cells through somatic cell nuclear transfer (Gurdon) or induced pluripotent stem cell techniques (Yamanaka). This suggests aged cells could potentially be made more youthful. 3. In 2014, Sebastiano's group proposed using a controlled partial/transient reprogramming approach to change the age of cells without altering cell identity. They published foundational work on this "epigenetic reprogramming of aging" (ERA) in 2020 using a cocktail of 6 factors delivered as mRNAs. Since then, several studies have validated the approach in various tissues (muscle, skin, brain etc.) and organisms (mice, rats, primates). 4. Turn Biotechnologies is working to translate ERA into clinical applications by combining the biological concept, mRNA delivery vehicles, and lipid nanoparticle (LNP) delivery systems. Using mRNA avoids genomic integration risks and allows fine control of factor expression. Certain factors like MECP2 have beneficial effects when pulsed briefly in combination with others, contrary to some misconceptions. 5. In dermatology, ERA shows promising results: - In primary human fibroblasts, it causes transcriptomic resetting with activation of ECM related genes, anti-inflammatory effects etc. Fibroblast identity genes are appropriately modulated. - In human skin biopsies cultured ex vivo, aging biomarkers are favorably altered after ERA treatment. - An in vivo human skin xenograft model was developed to test ERA delivery and efficacy over several weeks. 6. For immunology, ERA is being explored to rejuvenate T cells: - Treated T cells from old donors proliferate robustly in response to tumor cells without any change in cell identity markers. - They express lower levels of exhaustion markers and maintain better anti-tumor cytotoxic function compared to controls. 7. ERA has potential applications in many tissues like muscle, eye, cartilage, blood, and skin, with dermatology and immunology being the initial focus areas. 8. Delivery of mRNA remains a key challenge, especially achieving sufficient penetration. For skin, microneedling/injections are the near-term approach, with topical solutions being developed. Broader interest and investment in mRNA and gene therapies, catalyzed by COVID vaccines, is expected to accelerate progress on novel delivery systems. In conclusion, Sebastiano presented compelling evidence for ERA as a promising approach to partially reverse cellular aging through transient epigenetic reprogramming. By translating ERA into viable clinical therapies, Turn Bio aims to ultimately increase human healthspan. While delivery hurdles remain, the underlying biology appears sound and reproducible across contexts. ERA represents an exciting frontier in the fight against aging and age-related dysfunction.
Thank you for the great summary :)
Doing my first round of FMD. Primarily interested in anti cancer. Wondering if alternating the FMD with a vegan KETO would stress cancer cells more than just FMD. Example, month 1, FMD, month 2 Ketogenic diet (5 days).
Thanks. Very important. As a 69 yo male very encouraging as I exercise daily these last 12 years. Hba1c stays ~ 5% and hsCRP at <= 0.3 mg/L consistently. Will test IL-6 on morning before exercise to confirm it is low.
The research is absolutely stunning and many people are pending from any news regarding this study. However, I think the difference between targeting "degenerating" or "dead" retina cells in the end of the talk, could have worth a bit more time explanation. Also, do you plan a glaucoma model for the non-human primate test?
I've read his book lifespan couple weeks ago.. and thereafter I'm looking for his interviews
If he becomes my teacher, I’d be honored.
That is amazing!! I will like to hear more...l would like also read the book Lifespan!!😊by David!!
Pure NMN powder from donotage is really good.
I would like to know you said this company is going to make NMN Into a drug, how is that going to affect the rest of the people that you are educating on his benefits, to me that would limit it greatly as to who can get it?
I wonder if he has looked at senescent cells? Are they reduced as in senolytic therapies?
So VERY DISAPPOINTED that you are associated with the WEF. As we all know Klaus Schwab, Gates and the WEF are for depopulation of the world by billions!! Unless these supplements are only for the elites I am very hesitant to believe what is presented. Maybe the average "useless eater", as Klaus calls the general population is not supposed to hear this information or take the supplements!
Did I hear him claim to have dead lifted almost 1,000 pounds?? If so can't he hold a stationary Iron Cross on still rings with 100 extra pounds strapped around his waist?
Did he say, penguins are mammals?
It's a bit vague. Something like, "mammals can go long periods of fasting, LIKE these emperor penguins." So the way I read it is that; mammals can fast like penguins. Rather than penguins are mammals (which is incorrect of course, they are birds).
Methionine is better then acuall sam-e for creating sam e and having a anti depressive effect new studies show. Yet many years back aging docs pushed that methionine restriction increase lifespan. Wich makes no sense as methionine is a must for methylation and sam e creation.
I want to move to Singapore now.
Seems criminal that I can't make an informed decision by myself to use rapamycin and take the risks: the possible rewards seem worth it. The whole "we need rapalogues cuz pharma isn't interesting in rapamycin cuz it can't be patented is infuriating.
really the one person to ask a question was some girl who wanted to know what to study...great way to waste it...🙄
I can tell he is a toxic PI the way he talks about his student
She seemed very nervous, but she did a great job. Very interesting talk.
I have done the Prolon 5 day fasting mimicking diet and having the prescribed food made it possible to tolerate the diet because extreme hunger wasn't a factor. I believe in Dr. Longo's research.
I'm on a Six-Day fasting having about 1,000 calories in six days I drink olive oil +pollen spirulina and a handful of walnuts, 23 hours bed rest per day, I have a very small amount of high dense nutrition
Love this
Thank you very much for your valuable information ♥👍👍
What is the mechanism to revert age by applying four Yamanaka factors?
It's important to know basic underlying mechaminism resetting epigenetic setting cell. This will be found by some genius.
A very interesting talk and subject. I'm surprised so few seems to react on it. Only one comment, for example!
A price implies a selective process that perpetuates the cycle of disenfranchisement for those who have no substantial income beyond housing and food.
Unless the cost is covered by third-party payers, such as insurers, charities, and governments.
Wow, that is super-exciting research. Great talk.
Fantastic. Dr.Coray provides comprehensive evidences to prove this old prediction is true.
Bus drive surely could have waited one more question, you have David Sinclair on stage. Come on!
❤❤❤
This was most interesting! The connection between mitocondria and senescence is new to me, and seems to change things compleatly. The similarity between apoptosis and senescence is intriguing! I wonder what effect PhotoBioModulation would have on senescencent cells. Since that affects the mitocondria. Would it push them into apoptosis, into recovery, or neither?
♥️♥️♥️♥️♥️♥️♥️♥️♥️♥️♥️♥️