Пікірлер

• 
  @jameskajo8322 күн бұрын

  Wonderful presentation, how then do you work with microplastic pollution in aquatic environments please

• 
  @bilqeessaleeman30203 күн бұрын

  Good Presentation

• 
  @FATIMABAS-yb2ut3 күн бұрын

  good lecture

• 
  @npcperson215829 күн бұрын

  So cute. Sweet faces.

• 
  @haizha7112Ай бұрын

  Adorable🥰

• 
  @ShannonJCasey-jg4icАй бұрын

  Brilliant. Very sweet. Making me homesick.

• 
  @FulvioGaАй бұрын

  A lot of information in this video!

• 
  @ogbonnagoodnewsАй бұрын

  Commendable

• 
  @AMINUSIYAKAАй бұрын

  Thanks for the presentation.

• 
  @terenceiutzi40033 ай бұрын

  I take microplastic every day. The brand I prefer is Metamucil.

• 
  @zgrad20083 ай бұрын

  Насколько понимаю, в этой поляризационной пленке есть йод, который и создает поляризацию. Поэтому люди и кушают йод, как витамин.

• 
  @KechidKenzaa-gz9xc4 ай бұрын

  What the name of the this pls where i can find it

• 
  @clivebagshaw28054 ай бұрын

  What do you mean by "it"?

• 
  @KechidKenzaa-gz9xc4 ай бұрын

  That black circle, what's its name? I tried it today in the laboratory, but I did not find better results. Perhaps I was not able to imitate you well

• 
  @clivebagshaw28054 ай бұрын

  @@KechidKenzaa-gz9xc They are called O-rings obtainable at hardware stores or on-line (e.g. www.amazon.com/Assortment-Plumbing-Automotive-Mechanic-Connections/dp/B0BJJM91G4/ref=sr_1_19?crid=27NKCBIU57GXU&dib=eyJ2IjoiMSJ9.rMalqnjJK4ptQgIJLXaeol0CN2hMWmSikj0mL1o6aGlH5g0-8UIIJyiXwOtEuSjqxBKzqwW-IMcVi-_6pHMGryygvNELJ9HPp-0vgETRlN_inr_LJkckcwHABGCxr4WD1YOlBKwzdIFHapbaxmIKQghMH8lKh-Z-aK-GKxunq28ntR_F0x6W0id9ajHGwhK3aVH3o7b3rkDwG9otfOWrci5jI2GN2pDwC7mfw3j6JUg.-Gz4sNxGwVz7M4HdpxvjHgJhkWh9_xkTiSzZhAnAiXM&dib_tag=se&keywords=O+rings&qid=1711297114&sprefix=o+rings%2Caps%2C248&sr=8-19 What results were you looking for? The main challenge is making a waterproof seal. O-rings come in different thicknesses - the bigger the depth, the more volume you can examine but the image quality will be reduced.

• 
  @KechidKenzaa-gz9xc4 ай бұрын

  @@clivebagshaw2805 Thank you very much for the explanation. We will repeat the phytoplancton observation using exactly them

• 
  @robertvarner95195 ай бұрын

  Excellent demonstrations and explanations.

• 
  @legoworks-cg5hk5 ай бұрын

  Wouldn't a uv light be better?

• 
  @clivebagshaw28055 ай бұрын

  That depends on the fluorophore. We used this set up to look at Microplastics stained with Nile Red dye for which blue is best. For autofluoresence would be good.

• 
  @jshellenberger78765 ай бұрын

  - #POW #OOTUS #king

• 
  @BritishBeachcomber5 ай бұрын

  Is it a pet-tree or pee-tree dish? Lost in the pond? It is definitely pet-tree. Wanna fight about it?

• 
  @RadBudaspectrum-photography5 ай бұрын

  I have seen a beautiful images of crystallised paracetamol. I have filtered some paracetamol but crystals are nowhere near the same shape like the ones I have seen. Any clues?

• 
  @clivebagshaw28055 ай бұрын

  Sorry, I don't have any direct experience with paracetamol. I can only suggest slowing down crystal growth by reducing the evaporation rate (e.g. in a partially closed container)

• 
  @RadBudaspectrum-photography5 ай бұрын

  Thank you, I will certainly try that. The covering glass didn't really worked as the paracetamol cristals started to grow outside of the edge of covering piece. Do you know any other substances that grow interesting cristals? I've tried all typical household accessible chemicals. Take care

• 
  @clivebagshaw28055 ай бұрын

  I haven't explored crystals much but have seen some interesting patterns when sea water dries out on a slide sfmicrosociety.org/features/living-in-a-polarized-world@@RadBudaspectrum-photography

• 
  @Nextsession2715 ай бұрын

  Wow! I just found your video tutorial! This is 👍🏿 great! Thankn You. I subscribed 😊

• 
  @JohnnyLaps6 ай бұрын

  Evacuate!!

• 
  @user-bg6ig5dv8w7 ай бұрын

  hi I am thinking of quantifying microplastics using nile red dye in fluorescence spectrophotometer. Cannot find clear methodology need to be adopted for this. Do have any idea on the procedure need to be followed for this technique used for quantifying microplastics?

• 
  @clivebagshaw28057 ай бұрын

  It might be difficult to use a fluorescence spectrophotometer because Nile Red will emit a low background fluorescence from the solvent that might swamp that from the microplastics. Under a microscope with a thin sample (<2 mm) the stained plastic particles can easily be seen and quantified. Also with our own samples, some plankton are stained with Nile Red (especially zooplankton) and we can visually separate them from plastic, but in a bulk sample in a cuvette this would not be possible

• 
  @user-bg6ig5dv8w7 ай бұрын

  what about the nanoplastics, how can we quantify them?

• 
  @user-bg6ig5dv8w7 ай бұрын

  do you have any suggestions on how to use fluorescence spectrophotometer for quantification of microplastics?

• 
  @clivebagshaw28057 ай бұрын

  @@user-bg6ig5dv8w No. You would be better off using a cheap microscope with a blue LED light source than using a spectrophotometer that cannot distinguish the fluorescence from particles and solvent.

• 
  @erikabt89139 ай бұрын

  Very interesting, congratulations! One question, is it useful to observe microplastics inside the insects after having depigmented the cuticle? Thank you!

• 
  @riderinsanjose33710 ай бұрын

  Hello Clive. After having been away from the hobby for quite a while, I've noticed depression slides, or as I've known them as deepwell slides, are not as deep as I recall them to be. The O-ring spacer is an elegant solution to that problem. Thank you very much for putting this video up.

• 
  @voiceactorRU Жыл бұрын

  Thanks. What needs to be purchased for a regular light microscope? Under 20 dollars Thank you in advance

• 
  @clivebagshaw2805 Жыл бұрын

  Polarizing film sheet $9.40 www.amazon.com/gp/product/B004X3XFHU/ref=ppx_od_dt_b_asin_title_s00?ie=UTF8&psc=1 or two circularly polarizing filters at $9.99 each www.amazon.com/Fotasy-Multi-Resistant-Coating-Circular-Filter/dp/B00BAH9AX0/ref=sr_1_11?crid=98NRMY3NYDTZ&keywords=37+mm+polarizing+filter&qid=1684971202&s=electronics&sprefix=37+mm+polarizing+filter%2Celectronics%2C176&sr=1-11

• 
  @chir0pter Жыл бұрын

  How commonly can they be found off California? do they require subtropical waters being moved north?

• 
  @clivebagshaw2805 Жыл бұрын

  I have only seen them a couple of times (in 2021) - we sample weekly at the Santa Cruz wharf

• 
  @chir0pter Жыл бұрын

  @@clivebagshaw2805 I wonder if it was correlated with oceanographic metrics, or just abundance of a prey they like, or both?

• 
  @clivebagshaw2805 Жыл бұрын

  @@chir0pter The water temperature was 17.1 degrees C, which was near the maximium for that year and 1 degree warmer than the year before

• 
  @chir0pter Жыл бұрын

  @@clivebagshaw2805 cool, so perhaps a trip down to Sea of Cortez or Baja coast and one could find them?

• 
  @clivebagshaw2805 Жыл бұрын

  @@chir0pter No need to travel! An Erythropsidinium showed up in today's sample from the Santa Cruz wharf. Water had warmed up to 14.8 degrees (from 12.3 two weeks ago)

• 
  @ham1633 Жыл бұрын

  Hi. I am working on microplastics in my plant sample as well as soil sample. My plant sample is a very small aquatic plant. Its a macrophyte. And my aim is to count MPs adsorbed on its surface. Please guide me that how to count that samples? I am thinking to count it by micrscope. But you please tell me about more effective method. And also i wanna know about the particular amount of NaCl for particulat amount of soil sample in water. Please try to respond me as soon as possible. Because i am currently working on it.

• 
  @clivebagshaw2805 Жыл бұрын

  How are you detecting microplastics? How big are they? In favorable cases you can use automatic methods for counting: see www.mdpi.com/2673-4532/4/1/4 For flotation of plastics using NaCl, it is best to use saturated NaCl - it doesn't matter if you have excess salt as it will settle with the soil at the bottom of the tube, while most plastics will float.

• 
  @ham1633 Жыл бұрын

  @@clivebagshaw2805 appearantly there no Mps on the surfaces but they are detected by microscope adsorbed on my plant sample. For microplastics seperation from sediment sample is challenging for me. Also nile red dye and its protocol related tools are not available for me. For what size of MPs density seperation is effective because they are no larger microplastics that can be seen by naked eye. Also guide me please what to do after adding saturated salt. How to carry those invisible microplastics by density seperation and how to detect them? By simple electric light microscope? Because the only microscope i have is electric light microscope.

• 
  @clivebagshaw2805 Жыл бұрын

  @@ham1633 Microplastics usually float in saturated salt solution regardless of their size. After mixing NaCl to soil sample, allow the soil to settle, then suck off the surface layer with a pipette and transfer to a microscope slide. If there are no visible plastics, add some larger pieces of polythene to the sample to check the floatation method is working. Microplastics less than 1 mm in size are difficult to identify just by looking under a microscope. Try using cross-polarizers to examine the sample: kzread.info/dash/bejne/m4KCzcaBqdnOktY.html Polarizers can be obtained from Amazon: www.amazon.com/Polarizing-Film-Sheet-Gadget-Electronics/dp/B004X3XFHU/ref=sr_1_1_sspa?crid=9X8OOP59XNT1&keywords=polarizing+film+sheet&qid=1681402074&sprefix=polarizing+%2Caps%2C284&sr=8-1-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUEyM1BIUk04TVpKQUMxJmVuY3J5cHRlZElkPUEwODI3ODE0MzE1Ulo2WlcyV1E2TiZlbmNyeXB0ZWRBZElkPUEwMjQ5ODY0UVg0WDFUVVpDSVQ5JndpZGdldE5hbWU9c3BfYXRmJmFjdGlvbj1jbGlja1JlZGlyZWN0JmRvTm90TG9nQ2xpY2s9dHJ1ZQ== Plastic usually show up as bright or colored objects in polarized light - this method is not infallible, which is why Nile Red staining is a useful additional method. Research labs use infrared or Raman microscopy to be more certain particles are made of plastic.

• 
  @ham1633 Жыл бұрын

  @@clivebagshaw2805 Do i need to do chemical digestion after density seperation method? I mean Can i use chemical digestion for Microplastics obtaining from both plant and sediment sample.

• 
  @clivebagshaw2805 Жыл бұрын

  @@ham1633 You will probably need a digestion step for sediment samples. I have not done this for our plankton samples as we do not have lab facilities. We find Nile Red stains zooplankton, especially their membranes and fat stores, but they do not stain phytoplankton very well because the cell wall slows down Nile Red absorption. Nile Red does not stain fragmented fibers of cellulose, which helps distinguish them from synthetic fibers.

• 
  @sayrammukri2890 Жыл бұрын

  Can you please tell me the nile red molecular formula which is used, and also tell me that is the nile red dye is in powder form or liquid form, how did uh dilute it??

• 
  @clivebagshaw2805 Жыл бұрын

  The Nile Red dye was purchased from MP Biochemicals via VWR Ltd. A stock solution of 1 mg/mL in methanol was prepared. Alternatively, Nile Red was dissolved in 91% isopropyl alcohol (rubbing alcohol) available from a local pharmacy. 1 drop (0.02 ml) of Nile Red stock solution was added to 5 ml seawater sample. Formula is at www.mpbio.com/us/nile-red

• 
  @sayrammukri2890 Жыл бұрын

  But if I wanna purchase the nile red then to I have to know whether it same or not ... So please can you tell me the molecular formula of nile red from the bottle which is used??

• 
  @clivebagshaw2805 Жыл бұрын

  @@sayrammukri2890 There is no formula on the Nile Red bottle - just a molecular wt = 318.4. The formula of Nile Red is given en.wikipedia.org/wiki/Nile_red

• 
  @sayrammukri2890 Жыл бұрын

  Thank you that uh helped me, but tell yr Nile red dye was already in liquid form pr powder form...?? And then uh made stock solution by adding methanol?

• 
  @clivebagshaw2805 Жыл бұрын

  There was 1 mg of dry powder in the bottle as supplied and I added 1 ml of methanol to it, to make the stock solution. There is not enough to weigh out. When dealing with small amounts it is best to dissolve in the estimated volume and then check the concentration from the absorption spectrum if you want a more accurate value.

• 
  @ronaldbarrett3112 Жыл бұрын

  Excellent video, very informative. Thank you.

• 
  @dreamfunction4491 Жыл бұрын

  Excellent presentation sir. Thank you.

• 
  @johndubose6589 Жыл бұрын

  Nice, very simple!

• 
  @ioppdal Жыл бұрын

  Great!

• 
  @ElMitocondrio Жыл бұрын

  this is the video that i needed, thanks you!

• 
  @rodneyglover612 Жыл бұрын

  What percentage of ethanol was used

• 
  @clivebagshaw2805 Жыл бұрын

  In the video, we dissolved the Nile Red in 100% methanol to make the stock solution. We have also used 91% isopropyl alcohol (rubbing alcohol). We add around 20 ul of stock Nile Red to 5 ml seawater sample, so the final conc of alcohol is around 0.4%

• 
  @JustDux Жыл бұрын

  Looks like I can use (or make) dark field condenser, replace microscope LED with UV LED and put yellow film on cover slip

• 
  @clivebagshaw2805 Жыл бұрын

  That might work. I tried putting a blue filter in front of the built-in LED and using a dark field patch stop but it was not as bright as using a separate focused LED flashlight.

• 
  @eezyclsmooth9035 Жыл бұрын

  That was very impressive indeed. Dark field condensers, objectives and lamps are incredibly expensive?? It WORKS! Well done. I have both a stereo and compound scopes. I never knew this worked on a stereo scope!

• 
  @jugbrewer Жыл бұрын

  Great video! Would you get a better signal-to-noise ratio if you used a glass filter over the objective lenses instead of plastic? I imagine some of the blue light bouncing off the dish will cause autofluorescence in the plastic filter itself, reducing contrast in the images?

• 
  @clivebagshaw2805 Жыл бұрын

  The plastic filter may show some autofluorescence, but the main problem is that these cheap filters do not block all the blue light, so there is a high background of blue scattered light. Actually, this can be an advantage in this application because all objects can be seen either from their intrinsic scattering or from the yellow, orange or red fluorescence from bound Nile Red. It does however limit the detection of small weakly fluorescent objects.

• 
  @ruchisingh2177 Жыл бұрын

  ,🤞🤞🤞🤞😊

• 
  @md.abdullahebnrashid2 жыл бұрын

  How can I make a 250 gram ZnCl2 aqueous solution with a density of 1.55 g/cc? How much solid ZnCl2 salt and water do I need to make it (in gram)?

• 
  @clivebagshaw28052 жыл бұрын

  Please see email reply

• 
  @ldfreitas94372 жыл бұрын

  Greenway, AKA Greedway, LOST. Down went Measure D like Frazier!

• 
  @frankrimiccijr.3742 Жыл бұрын

  How do We light a fire under the RTC's ass? We have rail for tourists, Why cant the RTC get moving on this issue for residents?

• 
  @Sauron1912 жыл бұрын

  Is it possible to highly intensify the brightness of fluorescence of a given chemical?? I know this may sound a bit strange but I’m a big Star Wars fan and there is a huge market for custom made light sabers, these have polycarbonate blades with either a tri Cree LED in the hilt or a neo pixel strip running inside the blade (Please look up Neo Pixel light saver to see what I mean) with capabilities of colour changing … Now my theory for getting the blades to look a bit more ‘realistic’ (For the want of another term) than just an illuminated tube of plastic would be to add high intensity fluorescent chemicals and add an extra UV LED to the neo pixel strip, Now these blades would be colour locked to a chosen colour depending on the chemical added to the polycarbonate tube during manufacture unlike the standard neo pixel. So for example let’s say a red blade, the neo pixel strip would contain 3 red, 1 white & 1 high intensity UV (of a safe wavelength), orientated facing outwards from the epicentre of the polycarbonate tube with a defusing material of some kind The chemical would have to be translucent enough to allow the visible red light through the tube BUT the UV would make the chemical fluoresce (Highly, intensely hopefully IF the defusing material doesn’t hinder it) creating a red ‘hue’ around the blade … The same could be done with other chemicals to get other colours …. Does this sound feasible??

• 
  @clivebagshaw28052 жыл бұрын

  It should work if you can find the right fluorescent dye. This is the same principle used in washing powders that claim to be "whiter than white". The added brighteners absorb in the uv and fluoresce in the blue. However fluorescent light is relatively weak - it may only be effective in the dark and will not be as bright as colored LEDS.

• 
  @jeffreystrasser53732 жыл бұрын

  This video is very helpful. Thanks for posting!

• 
  @microuruguay2 жыл бұрын

  Super nice!

• 
  @4588ron2 жыл бұрын

  Thank you I am going to give it a go it looks very interesting

• 
  @tembukiso92922 жыл бұрын

  Hello thank you for this. I’m not sure if you’ll see my comment. I’m working on a project and I want to prove the presence of microplastics in food packaging, I’ll be sampling a typical plastic bag that usually contains hot soup (in Asia), I’m wondering how can I use this method because I have failed to acquire a spectroscopy and this is my only available resource. I’m thinking of boiling the plastic (using a protocol from a study that uses FTIR for analysis) and then analayze the boiled water with the miscroscope. I’m not sure if I’m making sense

• 
  @clivebagshaw28052 жыл бұрын

  I have sent a reply via email

• 
  @nafeesaali3539 Жыл бұрын

  Hello

• 
  @nafeesaali3539 Жыл бұрын

  I hve almost project to research how do you design to detect MP

• 
  @Darksagan2 жыл бұрын

  Nice. I have the same microscope.

• 
  @md.abdullahebnrashid2 жыл бұрын

  Peace be upon you. I wanna do separate microplastics from soil and do a small experiment on it.Can you please help me? Thanks!

• 
  @clivebagshaw28052 жыл бұрын

  If you suspend the soil sample in a saturated salt (NaCl) solution, the microplastics will mainly float while the soil will sink. ZnCl2 is even better but NaCl will do. See: drive.google.com/file/d/1mptyePBzVZjZZz2wg0-CcnLZh4SKVIzD/view?usp=sharing

• 
  @WarMarsM2 жыл бұрын

  Is there any particular yellow filter you recommend?

• 
  @clivebagshaw28052 жыл бұрын

  The ones I used are no longer available but these look similar: www.amazon.com/LingoFoto-Filter-Orange-Pockets-Cleaning/dp/B08ZK9J737/ref=sr_1_14?crid=1CTRB6MXPCN41&keywords=37mm%2Bacrylic%2Bcolor%2Bfilters&qid=1652151191&s=electronics&sprefix=37%2Bmm%2Bacrylic%2Bcolor%2Bfilters%2Celectronics%2C159&sr=1-14&th=1

• 
  @loocoo88772 жыл бұрын

  fast lil dudes, would cross polization reveal any crystals in them?

• 
  @clivebagshaw28052 жыл бұрын

  Some copepods show strong colors in their bodies under crossed polarizers, others appear evenly dark.

• 
  @Ardeact2 жыл бұрын

  I found polarization to reveal some interesting "tissue" like structures.

• 
  @haroldchester9042 жыл бұрын

  Just what I wanted - a clearly described method. Thanks.

• 
  @clivebagshaw28052 жыл бұрын

  Glad it helped!

• 
  @yonyon10022 жыл бұрын

  Going to be analysing microplastics in a sample of water but there is lots of dirt and so on in the water. Do you reccomend filtering it with some filter paper or will that affect the results.

• 
  @clivebagshaw28052 жыл бұрын

  Filtering will remove both microplastics and sediment in the water. It might be best to separate them by adding saturated salt (NaCl) to the sample to increase its density - most sediment will sink and most plastics will float on the surface. You can then pipette the surface layer to retrieve the microplastics. You need to do a control experiment with pure water as some salt samples contain microplastics.

• 
  @yonyon10022 жыл бұрын

  @@clivebagshaw2805 Thanks for the quick response! I read on an article outlining a similar method using nile red that ZnCl2 was used to help the microplastics float. Is this necessary or optional?

• 
  @clivebagshaw28052 жыл бұрын

  @@yonyon1002 Yes ZnCl2 is used in the lab because it is denser than NaCl and gives better separation. But for experiments with community scientists, NaCl is easier to acquire.

• 
  @yonyon10022 жыл бұрын

  @@clivebagshaw2805 what concentration of NaCl would you reccomend for this?

• 
  @clivebagshaw28052 жыл бұрын

  @@yonyon1002 Saturated NaCl should work. As the microplastics will float on the surface, having some undissolved NaCl at the bottom of the tube is not a problem.

• 
  @hellishnickolas69812 жыл бұрын

  Nice 👍