Yifan Cheng (UCSF & HHMI) 1: Single Particle Cryo-EM
Ғылым және технология
www.ibiology.org/biophysics/s...
Yifan Cheng overviews the principles of Cryo-EM, and describes how advances in this technique have allowed scientists to solve biological structures to atomic resolution.
Part 1: Single Particle Cryo-EM: Yifan Cheng overviews the principles of Cryo-EM, and describes how advances in this technique have allowed scientists to solve biological structures to atomic resolution.
Part 2: Single particle Cyro-EM of membrane proteins: Yifan Cheng shows how his laboratory has used Cryo-EM to study the atomic resolution of membrane proteins.
Talk Overview:
Cryo-Electron Microscopy (Cryo-EM) is a form of transmission electron microscopy in which a beam of electrons is transmitted through a frozen-hydrated sample, and is used to study the molecular structure of biological samples. In this lecture, Dr. Yifan Cheng overviews the principles of Cryo-EM, and describes how advances in this technique have allowed scientists to solve biological structures to atomic resolution.
In his second lecture, Cheng shows how his laboratory has used Cryo-EM to study the atomic resolution of membrane proteins. It is challenging to use conventional methods to study membrane protein structure, given that the 3D structure of most membrane proteins is dependent on their interaction with the phospholipid bilayer. Cheng describes how his laboratory has overcome these challenges to successfully solve the protein structure of the TRPV1 ion channel in different conformations at atomic resolution. He describes the benefits of using of amphipols, lipid nanodiscs, and Fab-assisted approaches to facilitate structural studies.
Speaker Biography:
Dr. Yifan Cheng is a professor of biochemistry and biophysics at the University of California, San Francisco, and a Howard Hughes Medical Institute Investigator. Cheng obtained his bachelor’s degree in Physics at Wuhan University in 1982, and completed his doctoral degree in Physics at the Chinese Academy of Sciences in 1991. In 2006, he joined the faculty of University of California, San Francisco, where his lab continues to use Cryo-EM to study the 3D structure of macromolecules.
Learn more about Cheng’s research at his lab website:
cryoem.ucsf.edu/index.html
Пікірлер: 29
Yifan is elected as member of national academy of sciences. Congrats!!!
This is mindblowing work. Absolutely incredible advancements. I'm a layman and don't really care much about biology, but the microscopy physics aspect is absolutely stunning. This technique and the single microcrystal electron diffraction technique using single electron counting imagers from angewandte chemie a couple years ago that allows the molecular structure of any small molecule to be determined ab initio is going to totally revolutionize biochem and materials science in the coming years.
I am only a beginner in Cryo-EM, however, I can understand most of the content in this talk due to the vivid explanation! Thanks a lot!
Very clear explanation about the problems and his solution .My previous view about was G.Jensen work ...astonishing work .Well do it ,good work.
Excellent, clear, brief, and very dynamic
I just saw it. It is a great talk and very clearly explained the Cryo-EM. I am a medicinal chemist and very excited to see the remarkable progresses made in the protein structural determinations. Do you have more updated new talks?
Such a good video. Great explanation!
Really helpful! Thanks for your every picture
Very clearly explained
Amazing Xie Xie!
Very nice. Would be useful to list a bunch of classical papers on the subject.
@murpholinox
3 жыл бұрын
The link to Cheng's research lab is down :(
@scicommlab
3 жыл бұрын
Fixed! Thanks for letting us know :-)
抢个沙发！
程老师牛啊！
@harvdaguy
4 жыл бұрын
Yifan Hu 胡老师牛！
@yifanhu1546
2 жыл бұрын
@@harvdaguy 哈老师更牛！
Put on ice. (7:47)
3-D reconstruction due to electron microscopy images is necessary. What is more necessary is to understand how quantum computation achieve many operations simultaneously, before we can know the complex evolutionary pattern of organs/cells.
@BIoodBath
5 жыл бұрын
What the fuck are you on about? Get your shit somewhere else.
relativistic correction is only 10%, it can be ignored for this talk
i tired to follow..before i switched it off
@DSAIRAMMSI
5 жыл бұрын
Start with Grant Jensen's 'Getting started in Cryo-EM'. This is an abridged version as stated by Dr.Cheng.
@Muonium1
4 жыл бұрын
I'm a layman and I found it very clear and easy to follow
@chemgoodinfo8642
Жыл бұрын
因为你是垃圾， 无法理解科学术语，，，，因为你是垃圾， 无法理解科学术语，，，，因为你是垃圾， 无法理解科学术语，，，，
Broken English, not impressive.
@sirranhaal3099
6 жыл бұрын
That was perfectly good English. --Native English speaker
@gaymo69
5 жыл бұрын
Ironically, i dont think you understand what broken english means. All of his statements are in perfect english.
@chemgoodinfo8642
Жыл бұрын
因为你是垃圾， 无法理解科学术语，，，，因为你是垃圾， 无法理解科学术语，，，，因为你是垃圾， 无法理解科学术语，，，，