Western blotting technique | Principle | Step by Step | In Hindi
This lecture explains about the western blotting technique step by step in Hindi.
The Western blot is meant for detecting proteins. The proteins are first purified from cells and then separated. During the separation you need to choose whether to keep the proteins natural or denature them. Denatured proteins are often easier to separate, but you might be looking for morphological variations. Separation is usually done on a gel followed by electrophoresis.
Often the gel will be stained after the electrophoresis so the proeins are visible. This is done to make sure that the electrophoresis worked.
Then the proteins are blotted from the gel and over to a membrane, usually nitrocellulose, Immediately after the blotting the membrane are blocked so no other proteins will attach themselves to the membrane.
After the blotting the membrane is induced so the proteins can be detected. Usually specific antibodies for the exact protein you are working with will be used.
A secondary antibody that can bind to the first antibody is then added and the secondary antibody has some kind of detection mechanism, either fluorescence, enzyme or color.
Please watch this video to have a clear idea about 2D gel electrophoresis.
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Your simplicity with words to manipulate makes you out of the box ..Nailed..Bold .. Respect
perfectly explained in lesser time, and i have understood all the concept in just 10 min that's amazing
Simple and classy way of explaining..... really you explained not just protocol but the concept behind it..thanks for the efforts......best wishes for upcoming videos.
Thank you for such an easy and accurate explanation.
Tomorrow is my presentation on western blotting technique in college, hope this helps :( I'm memorizing this 🙂
@ALLINONE-738
6 күн бұрын
💀 ??
Thank you madam. You told the theory part. I will be very happy if you show the practical also.
This video is really amazing and helped me to understand the western blot. Thank you very much 😊
Great Video Ma'am for explaining in the easiest way !
How can you explain such a complex method into such simple language ❤
I can understand your language very easy.... Thank you so much ma'am 🥰🥰
again thank you so much for all your videos
Thanks mamm you explained it shortly but the way of your explanation is attractive and best......
your teaching style is too good & u explained them all very well👌
On of my best channel simply explanation from Pakistan 😊
Very good ma'am, thank you so much Ma'am.
You helped me a lot...thank you so much..💗🙂
Amazing mam Really appreciated 👍👍
Mam thank u very much ur way of explaining awesome
Such a nice and informative video
THANK U VEY MUCH GOOD UNDERSTANDING 🙏👌👌🙂🙂
Oooooooo I found, what I was searching, thnx a lot mam, great
Informative 😊
As always very much helpful mam
Thank you mam bottom of my heart
Thanks for this..❤️💥
Thank you very much Ma'am! 😊
Amazing ma'am
Best teacher in the world mam😎😎😎😎
@BaaYo
4 жыл бұрын
🤗 love love
Mam do min to apko dekhne mein hi chale jate hain phir dhyan aata hain padhna bhi hain . Just joke yes i understand your concept easily Keep uploading ..
Thank you Mam You are Gem✨👍
You are great
Nice way of teaching ❤❤ appreciate able ❤
Thanks alot✌✌..
Good afternoon ma'am. Welcome again after long time.
@BaaYo
5 жыл бұрын
Thanks Uday😊
Too much easy explanation
Thanks bhuttt bda thankkuuu.....
Incidentally i saw ur video.. and now i m ur fan.. Well explained..u doing such a nyc work for hindi medium students.. Like me..thanks a lot. ☺️🙏🙏
@BaaYo
5 жыл бұрын
Thank you Akhilesh..
@namratasahu207
3 жыл бұрын
I also
Best of explain
Ma'am thank you. App bahut achche se samjhati h aur samjh me bhi asani se aa Jata h. Mai Aapke Sare video dekhta hu. Ma'am Corona virus pr bhi video banaiye.
i love your lecture and the best part is you explain in such a way that everyone can understand
@BaaYo
5 жыл бұрын
Thanks Saahikanta 😊
Thanks. Please make video on Northern and Southern blotting as well.🙏🙏
Thank you ma'am 🙏
Thank you so much
MAm m aapse Bhut pehle se padh rha hu.. Aaap Bhut accha Pdhate ho
Too good mam😚😚😚
Very nice ma'am
Thnkew so much 💓
Very nice
Wowww maza aa aagaya🤩🤩 bss mam vo Bata dijiye anti rabbit anti body use karenge uska mtb kya h ??
Thank you so much mem
Please make the lecture video on food microbiology's topics..
Good teacher ❤❤❤lov from Pakistan ❤❤
Please do make videos on molecular cloning
Thanks 👍
@BaaYo
10 ай бұрын
Thank you too
thanks mam for such a nice video.. you explain in very short and sweet way always. please make some videos on antibiotic resistance,and its types of efflux pumps. thanks again.. keep up..
@BaaYo
5 жыл бұрын
Noted Geeta.. Thanks for appreciation 🙏🏻😊
thank you mam
Thank you madam 😊
Very very Thanku mam
Nice maam.. Apke vedio se bht help milti h.. App microbiology se h maam ki biochemistry se
I am in class 12 this helps me a lot .....👍👍
1. Isolate protine 2 sample preparation 3. Sds page 4. Transfer on blotting pape Nitrocellulose and PVDF membrane
i have a question. why proteins are transferred from gel to membrane? function of secondary antibody?
Mam, please do next video on Mossbauer Spectroscopy.
Mam plz let's know difference between tris base and tris cl.
Thnx
Mam, I want to know, is it require to again activate the PVDF membrane after the transfer?
Mam i love u .... and your teaching technique ❤❣
Nice analysis mam
@BaaYo
5 жыл бұрын
Thank you 😊
thanks for your lecture 🇳🇵🇳🇵🇳🇵🇳🇵
Nice...
@BaaYo
5 жыл бұрын
Thank u akansha 😊
Madam,what kinds of protein detection method can be used in western blotting ?
Mam app bahut aacha samjhati hai. Mam please bioinformatics ke topic per video banaiye
Thq mam ✨🎈
Ma'am can you explain on the topic 'schich test'
Ty 💥
Well explained 💜 from 🇵🇰🇵🇰
Thanks mam...
@BaaYo
5 жыл бұрын
Thank u Sonali 😊
Madam you are amazing 👏 ❤ love you 😍
Mam you are the best thank you for your guidence mam I have a question what is the full form of BSA and mam please make a video on CCIEP Please mam
@BaaYo
4 жыл бұрын
Bovine serum albumin :)
Ma'am real time pcr and rt PCR na videos hoy to please upload
Hplc k interview questions pe eak complete video banaye pls.
platelet aggregation test maam ,can u plz share
Jazakallah mam thnx
Thanku mam
Why don't we make primary antibody labelled ?
Nice video
@BaaYo
5 жыл бұрын
Thank you Narender 😊
@narender444
5 жыл бұрын
BaaYo Dear Madam I had applied in NIPER for VU-VIS spectroscopy, GC and HPLC chromatography Practical training short courses but there is no replay from NIPER. Can u please help me to recommend other Institutions near Chandigrah who provide me such practical training. Waiting for ur reply
Mam lc-ms pe bhi video bnaye n.
Nice ma'am .. plz make a video on cell cycle regulation
@BaaYo
5 жыл бұрын
Sure..will try to upload before 16th june..
@paramitachakravarty2714
5 жыл бұрын
Thank you so much ma'am
What is the limitation of this technique mam ???
This video of u is different your voice is more energetic Than your before videos Nd looks like u were excited!
@BaaYo
5 жыл бұрын
So you find it good or not?😊
@YogeshKumar-js7tx
5 жыл бұрын
I dont know but your serious voice i liked that nd ur cool apperance i guess😅in older videos
Mam give me a video on ELISA TECHNIQUE
Mam please make on video of michaelis mention equation( most imt part of enzyme).
👌
Mam far western blotting and eastern blotting and zooblotting plz explain
Mam please make a video on eastern blotting
Mam plz ICP OES pr lacture video
❤️🔥
Anti mouse ya anti Rabbit kya hota hai wese
Mam wat is antimouse antibody...and how these are developed
@Thesiblingshow4
5 жыл бұрын
Please go through this link for detail : www.thermofisher.com/in/en/home/life-science/antibodies/secondary-antibodies/secondary-antibodies-target-species/anti-mouse-secondary-antibodies.html