Western blotting technique | Principle | Step by Step | In Hindi

This lecture explains about the western blotting technique step by step in Hindi.
The Western blot is meant for detecting proteins. The proteins are first purified from cells and then separated. During the separation you need to choose whether to keep the proteins natural or denature them. Denatured proteins are often easier to separate, but you might be looking for morphological variations. Separation is usually done on a gel followed by electrophoresis.
Often the gel will be stained after the electrophoresis so the proeins are visible. This is done to make sure that the electrophoresis worked.
Then the proteins are blotted from the gel and over to a membrane, usually nitrocellulose, Immediately after the blotting the membrane are blocked so no other proteins will attach themselves to the membrane.
After the blotting the membrane is induced so the proteins can be detected. Usually specific antibodies for the exact protein you are working with will be used.
A secondary antibody that can bind to the first antibody is then added and the secondary antibody has some kind of detection mechanism, either fluorescence, enzyme or color.
Please watch this video to have a clear idea about 2D gel electrophoresis.
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#BaaYo #WesternBlotting #Protein

Пікірлер: 185

  • @BaaYo
    @BaaYo11 ай бұрын

    To access notes and practice questions, please join our Telegram channel t.me/baayo_official

  • @ManpreetSingh-bn8hu
    @ManpreetSingh-bn8hu5 жыл бұрын

    Your simplicity with words to manipulate makes you out of the box ..Nailed..Bold .. Respect

  • @mantastic7666
    @mantastic76662 жыл бұрын

    perfectly explained in lesser time, and i have understood all the concept in just 10 min that's amazing

  • @manojsharma-vn4ij
    @manojsharma-vn4ij3 жыл бұрын

    Simple and classy way of explaining..... really you explained not just protocol but the concept behind it..thanks for the efforts......best wishes for upcoming videos.

  • @moizahmed6149
    @moizahmed61492 жыл бұрын

    Thank you for such an easy and accurate explanation.

  • @KARZDAAR_MUSIC
    @KARZDAAR_MUSIC2 жыл бұрын

    Tomorrow is my presentation on western blotting technique in college, hope this helps :( I'm memorizing this 🙂

  • @ALLINONE-738

    @ALLINONE-738

    6 күн бұрын

    💀 ??

  • @771012pr
    @771012pr3 жыл бұрын

    Thank you madam. You told the theory part. I will be very happy if you show the practical also.

  • @zaisharandhawa7603
    @zaisharandhawa76033 ай бұрын

    This video is really amazing and helped me to understand the western blot. Thank you very much 😊

  • @BruceWayne-rb3wq
    @BruceWayne-rb3wq4 жыл бұрын

    Great Video Ma'am for explaining in the easiest way !

  • @scienceandfiction7108
    @scienceandfiction71086 ай бұрын

    How can you explain such a complex method into such simple language ❤

  • @nazminasultana6745
    @nazminasultana67453 жыл бұрын

    I can understand your language very easy.... Thank you so much ma'am 🥰🥰

  • @sashikantaparida9763
    @sashikantaparida97635 жыл бұрын

    again thank you so much for all your videos

  • @ArmanKhan-nv5oo
    @ArmanKhan-nv5oo5 жыл бұрын

    Thanks mamm you explained it shortly but the way of your explanation is attractive and best......

  • @sidrazafar3635
    @sidrazafar36353 жыл бұрын

    your teaching style is too good & u explained them all very well👌

  • @shehzadahmad5279
    @shehzadahmad5279 Жыл бұрын

    On of my best channel simply explanation from Pakistan 😊

  • @rajatmondal749
    @rajatmondal7494 жыл бұрын

    Very good ma'am, thank you so much Ma'am.

  • @learnwithme1652
    @learnwithme16522 жыл бұрын

    You helped me a lot...thank you so much..💗🙂

  • @anjalibhatt4710
    @anjalibhatt47103 жыл бұрын

    Amazing mam Really appreciated 👍👍

  • @ManiSharma-dn2rm
    @ManiSharma-dn2rm4 жыл бұрын

    Mam thank u very much ur way of explaining awesome

  • @saumyajyotsna8281
    @saumyajyotsna82812 жыл бұрын

    Such a nice and informative video

  • @JYOtiRaNJanMANgaRaj
    @JYOtiRaNJanMANgaRaj5 ай бұрын

    THANK U VEY MUCH GOOD UNDERSTANDING 🙏👌👌🙂🙂

  • @sultanmehmood5022
    @sultanmehmood50222 жыл бұрын

    Oooooooo I found, what I was searching, thnx a lot mam, great

  • @umarfarooq-zu3ov
    @umarfarooq-zu3ov5 жыл бұрын

    Informative 😊

  • @harshshukla1547
    @harshshukla15473 жыл бұрын

    As always very much helpful mam

  • @mridulasoni9335
    @mridulasoni93352 жыл бұрын

    Thank you mam bottom of my heart

  • @tanusingh1390
    @tanusingh13902 жыл бұрын

    Thanks for this..❤️💥

  • @dikshantkumar7660
    @dikshantkumar76602 жыл бұрын

    Thank you very much Ma'am! 😊

  • @rachna4140
    @rachna41403 жыл бұрын

    Amazing ma'am

  • @prarthanabidkar3675
    @prarthanabidkar36754 жыл бұрын

    Best teacher in the world mam😎😎😎😎

  • @BaaYo

    @BaaYo

    4 жыл бұрын

    🤗 love love

  • @gschauhan122
    @gschauhan1222 жыл бұрын

    Mam do min to apko dekhne mein hi chale jate hain phir dhyan aata hain padhna bhi hain . Just joke yes i understand your concept easily Keep uploading ..

  • @Faizabasar
    @Faizabasar2 жыл бұрын

    Thank you Mam You are Gem✨👍

  • @gopanandikes9208
    @gopanandikes92085 жыл бұрын

    You are great

  • @user-xz7mx6kt4f
    @user-xz7mx6kt4f2 ай бұрын

    Nice way of teaching ❤❤ appreciate able ❤

  • @sayambabu7365
    @sayambabu73653 жыл бұрын

    Thanks alot✌✌..

  • @korangaji6673
    @korangaji66735 жыл бұрын

    Good afternoon ma'am. Welcome again after long time.

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thanks Uday😊

  • @loardsofcooking4537
    @loardsofcooking45374 жыл бұрын

    Too much easy explanation

  • @mohinitiwari4775
    @mohinitiwari47753 жыл бұрын

    Thanks bhuttt bda thankkuuu.....

  • @akhileshkumarmaurya1240
    @akhileshkumarmaurya12405 жыл бұрын

    Incidentally i saw ur video.. and now i m ur fan.. Well explained..u doing such a nyc work for hindi medium students.. Like me..thanks a lot. ☺️🙏🙏

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thank you Akhilesh..

  • @namratasahu207

    @namratasahu207

    3 жыл бұрын

    I also

  • @mohdmuaz2763
    @mohdmuaz27634 жыл бұрын

    Best of explain

  • @sureshgoswami177
    @sureshgoswami1774 жыл бұрын

    Ma'am thank you. App bahut achche se samjhati h aur samjh me bhi asani se aa Jata h. Mai Aapke Sare video dekhta hu. Ma'am Corona virus pr bhi video banaiye.

  • @sashikantaparida9763
    @sashikantaparida97635 жыл бұрын

    i love your lecture and the best part is you explain in such a way that everyone can understand

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thanks Saahikanta 😊

  • @aaquib608
    @aaquib6085 жыл бұрын

    Thanks. Please make video on Northern and Southern blotting as well.🙏🙏

  • @sakshishukla03
    @sakshishukla033 жыл бұрын

    Thank you ma'am 🙏

  • @takini4918
    @takini49182 жыл бұрын

    Thank you so much

  • @Rahulkumar-zp2vd
    @Rahulkumar-zp2vd2 жыл бұрын

    MAm m aapse Bhut pehle se padh rha hu.. Aaap Bhut accha Pdhate ho

  • @rinkitewatia7632
    @rinkitewatia76324 жыл бұрын

    Too good mam😚😚😚

  • @anupamsingh1329
    @anupamsingh13294 жыл бұрын

    Very nice ma'am

  • @Sadiqaaa374
    @Sadiqaaa3743 жыл бұрын

    Thnkew so much 💓

  • @lifeisbeautiful499
    @lifeisbeautiful4994 жыл бұрын

    Very nice

  • @pratibhagupta8816
    @pratibhagupta88163 жыл бұрын

    Wowww maza aa aagaya🤩🤩 bss mam vo Bata dijiye anti rabbit anti body use karenge uska mtb kya h ??

  • @dhruvmehta1963
    @dhruvmehta19634 жыл бұрын

    Thank you so much mem

  • @sheljagupta6519
    @sheljagupta65195 жыл бұрын

    Please make the lecture video on food microbiology's topics..

  • @wajihayaqoob6235
    @wajihayaqoob62356 ай бұрын

    Good teacher ❤❤❤lov from Pakistan ❤❤

  • @prajwalgowda3786
    @prajwalgowda37865 жыл бұрын

    Please do make videos on molecular cloning

  • @amitsinghbhadoriya6318
    @amitsinghbhadoriya631811 ай бұрын

    Thanks 👍

  • @BaaYo

    @BaaYo

    10 ай бұрын

    Thank you too

  • @geetarani2875
    @geetarani28755 жыл бұрын

    thanks mam for such a nice video.. you explain in very short and sweet way always. please make some videos on antibiotic resistance,and its types of efflux pumps. thanks again.. keep up..

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Noted Geeta.. Thanks for appreciation 🙏🏻😊

  • @papridas5542
    @papridas55425 жыл бұрын

    thank you mam

  • @nikitampmaurya
    @nikitampmaurya3 жыл бұрын

    Thank you madam 😊

  • @yogeshanjansinger2872
    @yogeshanjansinger28724 жыл бұрын

    Very very Thanku mam

  • @sumanpandeysumanpandey7317
    @sumanpandeysumanpandey73173 жыл бұрын

    Nice maam.. Apke vedio se bht help milti h.. App microbiology se h maam ki biochemistry se

  • @psycho_PQR
    @psycho_PQR2 жыл бұрын

    I am in class 12 this helps me a lot .....👍👍

  • @englishspoken8168
    @englishspoken81689 ай бұрын

    1. Isolate protine 2 sample preparation 3. Sds page 4. Transfer on blotting pape Nitrocellulose and PVDF membrane

  • @muzamilarshad2671
    @muzamilarshad26713 жыл бұрын

    i have a question. why proteins are transferred from gel to membrane? function of secondary antibody?

  • @kkk-jp3zx
    @kkk-jp3zx5 жыл бұрын

    Mam, please do next video on Mossbauer Spectroscopy.

  • @MrFilu13
    @MrFilu135 жыл бұрын

    Mam plz let's know difference between tris base and tris cl.

  • @TOTALGVGAMEROFFICIAL
    @TOTALGVGAMEROFFICIAL2 жыл бұрын

    Thnx

  • @shivanivarshney2500
    @shivanivarshney2500 Жыл бұрын

    Mam, I want to know, is it require to again activate the PVDF membrane after the transfer?

  • @riteshgupta334
    @riteshgupta3344 жыл бұрын

    Mam i love u .... and your teaching technique ❤❣

  • @ramchandrasuthar2848
    @ramchandrasuthar28485 жыл бұрын

    Nice analysis mam

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thank you 😊

  • @bishalbhandari9242
    @bishalbhandari9242 Жыл бұрын

    thanks for your lecture 🇳🇵🇳🇵🇳🇵🇳🇵

  • @akanksha8908
    @akanksha89085 жыл бұрын

    Nice...

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thank u akansha 😊

  • @aniketsarkardava
    @aniketsarkardava9 ай бұрын

    Madam,what kinds of protein detection method can be used in western blotting ?

  • @vinodkushwaha3258
    @vinodkushwaha32584 жыл бұрын

    Mam app bahut aacha samjhati hai. Mam please bioinformatics ke topic per video banaiye

  • @mithleshkurre7685
    @mithleshkurre7685 Жыл бұрын

    Thq mam ✨🎈

  • @ImranKhan-xl8my
    @ImranKhan-xl8my2 жыл бұрын

    Ma'am can you explain on the topic 'schich test'

  • @ramdev822
    @ramdev8222 жыл бұрын

    Ty 💥

  • @waqasahmadkhan1690
    @waqasahmadkhan16902 жыл бұрын

    Well explained 💜 from 🇵🇰🇵🇰

  • @sonalithakur3468
    @sonalithakur34685 жыл бұрын

    Thanks mam...

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thank u Sonali 😊

  • @rabianoor1589
    @rabianoor15892 жыл бұрын

    Madam you are amazing 👏 ❤ love you 😍

  • @priyankamishra732
    @priyankamishra7324 жыл бұрын

    Mam you are the best thank you for your guidence mam I have a question what is the full form of BSA and mam please make a video on CCIEP Please mam

  • @BaaYo

    @BaaYo

    4 жыл бұрын

    Bovine serum albumin :)

  • @bhaktibhudiya9983
    @bhaktibhudiya99833 жыл бұрын

    Ma'am real time pcr and rt PCR na videos hoy to please upload

  • @nitkartchanel1831
    @nitkartchanel18315 жыл бұрын

    Hplc k interview questions pe eak complete video banaye pls.

  • @surajdesai8568
    @surajdesai85682 жыл бұрын

    platelet aggregation test maam ,can u plz share

  • @SMS.WAHEED
    @SMS.WAHEED4 жыл бұрын

    Jazakallah mam thnx

  • @sabreenbanosabreen5908
    @sabreenbanosabreen59083 жыл бұрын

    Thanku mam

  • @nitishdogra1170
    @nitishdogra11704 жыл бұрын

    Why don't we make primary antibody labelled ?

  • @narender444
    @narender4445 жыл бұрын

    Nice video

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Thank you Narender 😊

  • @narender444

    @narender444

    5 жыл бұрын

    BaaYo Dear Madam I had applied in NIPER for VU-VIS spectroscopy, GC and HPLC chromatography Practical training short courses but there is no replay from NIPER. Can u please help me to recommend other Institutions near Chandigrah who provide me such practical training. Waiting for ur reply

  • @varsharawat6581
    @varsharawat65815 жыл бұрын

    Mam lc-ms pe bhi video bnaye n.

  • @paramitachakravarty2714
    @paramitachakravarty27145 жыл бұрын

    Nice ma'am .. plz make a video on cell cycle regulation

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    Sure..will try to upload before 16th june..

  • @paramitachakravarty2714

    @paramitachakravarty2714

    5 жыл бұрын

    Thank you so much ma'am

  • @mdnaimsk7568
    @mdnaimsk75684 жыл бұрын

    What is the limitation of this technique mam ???

  • @YogeshKumar-js7tx
    @YogeshKumar-js7tx5 жыл бұрын

    This video of u is different your voice is more energetic Than your before videos Nd looks like u were excited!

  • @BaaYo

    @BaaYo

    5 жыл бұрын

    So you find it good or not?😊

  • @YogeshKumar-js7tx

    @YogeshKumar-js7tx

    5 жыл бұрын

    I dont know but your serious voice i liked that nd ur cool apperance i guess😅in older videos

  • @prasadchambhare5736
    @prasadchambhare57364 жыл бұрын

    Mam give me a video on ELISA TECHNIQUE

  • @knowledgejunction1643
    @knowledgejunction16435 жыл бұрын

    Mam please make on video of michaelis mention equation( most imt part of enzyme).

  • @ammaralatif3004
    @ammaralatif3004 Жыл бұрын

    👌

  • @Shyam_ladli668
    @Shyam_ladli6683 жыл бұрын

    Mam far western blotting and eastern blotting and zooblotting plz explain

  • @sheetalsoni6065
    @sheetalsoni60653 жыл бұрын

    Mam please make a video on eastern blotting

  • @abdulwahid-rb8ug
    @abdulwahid-rb8ug5 жыл бұрын

    Mam plz ICP OES pr lacture video

  • @icshahid
    @icshahid3 жыл бұрын

    ❤️🔥

  • @OdiaSongLyrics
    @OdiaSongLyrics3 жыл бұрын

    Anti mouse ya anti Rabbit kya hota hai wese

  • @MrFilu13
    @MrFilu135 жыл бұрын

    Mam wat is antimouse antibody...and how these are developed

  • @Thesiblingshow4

    @Thesiblingshow4

    5 жыл бұрын

    Please go through this link for detail : www.thermofisher.com/in/en/home/life-science/antibodies/secondary-antibodies/secondary-antibodies-target-species/anti-mouse-secondary-antibodies.html