Two different strategies we can use to identify proteins with mass spectrometry.
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Пікірлер: 20
@sallycha7952Ай бұрын
The very best concise explanation! Thank you.
@96Regine2 жыл бұрын
I couldn't find a nicer video with this simplicity and great explanation for my students! And yes, Dr. Kelleher has done some great job, that guy is the Top Down beast!!! Thank you
@arnolddowney98073 жыл бұрын
Honestly, great job! This was really well-done, both the explanations and the video and images to go with it. Just the right amount of humor too to shake it up. Off to check out the rest of your vids to help prepare for my final exam.
@enavigator38213 жыл бұрын
Thank you for the simplicity of your videos
@lukassch8773 Жыл бұрын
Thank you for the Explanation :) Pure gold to find it in my Master's exam phase!
@jikang71333 жыл бұрын
I really love this one!!
@TanTan-ch3vq3 жыл бұрын
You are the best, professor
@profwatad3 ай бұрын
really good explanation, thank you:)
@ornithology112 жыл бұрын
Godlike taste in music Sir.
@sarahajian81027 ай бұрын
Thank you this was very useful
@Automobilestats2 жыл бұрын
thankyou
@seanfitzpatrick83853 жыл бұрын
Is their a superior protein like whey, casein, plant? What is the difference between up regulated and down regulated protein?
@TheTrailGlider3 жыл бұрын
Can you talk about Quantum SI's silicon chip they claim can do single-molecule protein sequencing? I am interested in this as an investor and have been watching your proteomics videos to try and learn more about this field.
@shortchemistry7927
3 жыл бұрын
Nice topic... as someone in the proteomics field, I'd love to see the day where high throughput, single molecule sequencing is routine. But protein sequencing is very different than gene sequencing. Proteomics will always be inherently more difficult, particularly when it comes to sample handling. Then again, as someone who has also founded a company in proteome sample preparation, perhaps my opinion is biased.
@bgayatri11 Жыл бұрын
Hi Prof, in one part of the video you said that the z for the m/z needs to be identified. How is that done?
@gokudegrees
7 ай бұрын
Hey, the recorded m/z = x = \frac{M + n\phi}{n}. for one peak to the left, y = \frac{M + (n+1)\phi}{n+1}. Thus, you can do some simplification and you can get that n, which is the number of charges, n = \frac{y-\phi}{x-y}
@parthibanezhumalai2949 Жыл бұрын
Hi, How to determine molecular weight of the purified protein using tandem MS.
@alandoucette9997
Жыл бұрын
If you measure the intact protein (top down), then the mass can be determined by determining the charge state through a deconvolution calculation. However, in a bottom up experiment, we are relying on other information. The assumption is that "somebody" has already studied this particular protein. They know the precise amino acid sequence, and therefore they know the mass. In bottom up, we identify just a piece of the protein by tandem MS. However, that piece maps onto the original (intact) protein. This information is available for millions of different proteins, in web databases.
Пікірлер: 20
The very best concise explanation! Thank you.
I couldn't find a nicer video with this simplicity and great explanation for my students! And yes, Dr. Kelleher has done some great job, that guy is the Top Down beast!!! Thank you
Honestly, great job! This was really well-done, both the explanations and the video and images to go with it. Just the right amount of humor too to shake it up. Off to check out the rest of your vids to help prepare for my final exam.
Thank you for the simplicity of your videos
Thank you for the Explanation :) Pure gold to find it in my Master's exam phase!
I really love this one!!
You are the best, professor
really good explanation, thank you:)
Godlike taste in music Sir.
Thank you this was very useful
thankyou
Is their a superior protein like whey, casein, plant? What is the difference between up regulated and down regulated protein?
Can you talk about Quantum SI's silicon chip they claim can do single-molecule protein sequencing? I am interested in this as an investor and have been watching your proteomics videos to try and learn more about this field.
@shortchemistry7927
3 жыл бұрын
Nice topic... as someone in the proteomics field, I'd love to see the day where high throughput, single molecule sequencing is routine. But protein sequencing is very different than gene sequencing. Proteomics will always be inherently more difficult, particularly when it comes to sample handling. Then again, as someone who has also founded a company in proteome sample preparation, perhaps my opinion is biased.
Hi Prof, in one part of the video you said that the z for the m/z needs to be identified. How is that done?
@gokudegrees
7 ай бұрын
Hey, the recorded m/z = x = \frac{M + n\phi}{n}. for one peak to the left, y = \frac{M + (n+1)\phi}{n+1}. Thus, you can do some simplification and you can get that n, which is the number of charges, n = \frac{y-\phi}{x-y}
Hi, How to determine molecular weight of the purified protein using tandem MS.
@alandoucette9997
Жыл бұрын
If you measure the intact protein (top down), then the mass can be determined by determining the charge state through a deconvolution calculation. However, in a bottom up experiment, we are relying on other information. The assumption is that "somebody" has already studied this particular protein. They know the precise amino acid sequence, and therefore they know the mass. In bottom up, we identify just a piece of the protein by tandem MS. However, that piece maps onto the original (intact) protein. This information is available for millions of different proteins, in web databases.
it is hard to pronounce PTM lol
bottom... =)