I'm working on my supply orders for school next year and you just saved me a lot of money. Thanks for the great information.

Thanks! Great video. So helpful for teachers without much budget!!!

rich content as always - thank you!

Pretty awesome info for the mad scientist on a budget ^_^

It's worth adding that previously reagents like Dragendorff or ninhydrin mentioned in your previous videos work beautifuly with TLC, giving an extra dimension for identification of substances. And even without reagents you can make nice separations eg. dyes and inks (which is used in forensic investigations of documents when there's a suspicion of forgery).

Excellent video.

you are a legend, ty!

nice video!

do you have a website where these instructions are listed? That would be awesome!

very good!

Is it possible to use powdered granules from silica gel packs that come in a vitamin bottle or with your new pair of shoes (you know these desiccant packs). Also is it possible to use the immobilized phase you detail with highly hydrophobic (oily, resinous etc) compounds.

Interesting. I wander how well your alumina/plaster mix would perform in a homemade HPLC-like column...

1.25ml per gram alumina and 15% of binder ???????????? so 1.25 grams of water to 1 gram of alumina to 0.3375 grams of binder or 1.25 grams of water to 1 gram of alumina to 0.15 grams of binder

@pauldell6984

8 ай бұрын

did you ever solve the mystery?

what would u use for colouring as most tlc use fluorescent??

Hey RBT thanks for putting this video up! I use this everyday in lab (along with flash chromatography). I had been wondering what the binding agent was (I would think that the solvents would dissolve the binder). I think it would be cool if you talked a little about the principle of TLC bc most people won't have the solvents, uv lamp, or stains necessary. Cheers!

Can i use calcined alumina or fumed silica ?

what are the amounts again?

good blese

He mentioned that if we don't have access to alumina we can also use cornstarch or talcum powder. So can I use just about any type/brand of cornstarch or talcum powder or do I have to look for something specific? And can I just use it directly or is there some sort of process before? Hoping for a clear answer (I need to do this for a school project)

pretty interesting

I want to make TiO2 plates for experimenting with organic solar, i assume the process is mostly the same. Would you recommend the same binder? What else? What about sodium silicate?

How much does the binder affect retention? Is it possible to use this technique, using the same binder, with polymer-based reversed-phase media? Thanks for the great demo!

sweet

clever

if so made plate will glow in UV ?

@TheWanderingChemist

6 жыл бұрын

A slide prepared this way will not fluoresce or glow unter UV irradiation by itself (unless your substance is a fluorophore itself), for that you will need a Mn-doped Zn2SiO4 layer which will absorb UV light at 254 nm www.sigmaaldrich.com/catalog/product/sial/02554?lang=de®ion=DE

@petevenuti7355

10 ай бұрын

No, it's what absorbs into it that does or does not glow. Take several different color highlighter markers and mix up the colors in a small well (a slot as long as the width of the slide that's about 2-5 mm deep or so) dip the edge in , and the different colors will separate into bands and glow under UV as they climb up the slide before they dry.

kewl

Fail, I've accidentaly arrived at this video while having the TLC plate in front of me.

looks good, hey if it costs less to make them, make them yourself and sell them online for just under the store price and make a small buck =]