I cannot stress ENOUGH how helpful this was. Thank you so much

@abmgood

3 жыл бұрын

Hello there, We are really glad to hear that you found the video helpful! :) Thanks so much for watching!

A brilliant, clear and comprehensive video!

I've seen these topics being explained many times but never in such a clear and direct way. Thank you!

@abmgood

3 жыл бұрын

Thank you, Antonio! We are really glad that you found the video helpful ;)

Thank you very much for that material! I had to perform RNA-seq but I didn't fully understand it and I was intimidating to ask my coworkers for explanation and you sir saved me! Your explanation is so clear and without rush. Thank you!

@abmgood

3 жыл бұрын

Hello there, We are really glad to hear that you found the video helpful! ;) Don't forget to check out other videos on our channel too! Thanks for watching :)

I struggled understanding RNA-seq but this presentation made it super helpful and easy to follow! Now I have a much better idea. Thank you!

@abmgood

3 жыл бұрын

Glad you found the video helpful! :) Thanks for watching!

This is the best I have seen so far on RNA-seq... Thank you so much, sensei.

@abmgood

12 күн бұрын

Glad it was helpful!

Excellent work. Thanks so much for sharing.

This is the best explanation on RNAseq. Subscribed and will watch more videos! Thank you very much! This is so very helpful!

@abmgood

2 жыл бұрын

Hello Sage, Glad you found the video helpful! ;) Thanks for watching and stay tuned for upcoming videos!

I am more of a visual leaner and this greatly helped me. Massive thanks

@abmgood

3 жыл бұрын

Hello Bamu, We are so glad to hear that you found the video helpful! :) Thanks for watching!

Very helpful, thanx!!

The best explanation of RNA seq! Much more clear than my lectures.

@abmgood

2 жыл бұрын

Thanks for watching! :) Glad you found the video helpful!

easily understandable and thank you for this this very informative video. is there any ways detect the circularRNA is QC step?

This is the best introduction ever! thank you

@abmgood

3 жыл бұрын

Thanks so much for watching! :)

very nicely explained thanks a lot sir, but even after sign up i cant able to get ppt of this webinar please help me in this regard thank you...

Thank you so much for clarifying such a hard topic in a simple language. It was very helpful.

@abmgood

8 ай бұрын

Glad it was helpful!

Hi this video was useful for me.thank you i just have a question what is the replicants mean in sequencing?

Thank you so much. My professor is a mess trying to explain this, and with this video I understood inmediatly.

@abmgood

3 жыл бұрын

Glad to hear that you found the video helpful! :)

Thank you for the wonderful visual+explanation! 15:53 May I know does the fragmentation occur before cDNA synthesis or after? I look through the protocol from Illumina they fragmented the RNA before cDNA synthesis

Very good guide.

@abmgood

13 күн бұрын

We're glad to hear that!

Great webinar, thanks a lot !! How do I identify a novel transcript? For example, how would I know I discovered a new lncRNA??

@abmgood

4 жыл бұрын

Thanks for watching, Diego! To answer your question, if a transcript has no gene annotation, compared to the reference genome/transcriptome, this would likely represent a novel transcript. Keep in mind, though, that additional validation would be suggested for this.

Thank you !!!

I want to know what (amplicon sequence variant) ASV data means. What do the headers ASV1, ASV2, ...etc. mean? Where can I find such explanation?

Thank you for this webinar. I have a question regarding the Quality Control before proceeding to the actual RNA seq. You have mentioned the Qubit, Bioanalyzer, and qPCR. Is it a step by step process or can use either one of this technology to assure the quality of the sample? Thank you in advance.

@abmgood

3 жыл бұрын

Each of these tools have different roles where Qubit is used to determine the RNA concentration in a sample. This is followed by using an Agilent Bioanalyzer to determine the RNA Integrity Number. The Bioanalyzer has a limited detection range and the Qubit value helps to determine if dilution is necessary prior to running on the Bioanalyzer. If your RNA concentration is usually within range, then the Qubit assay can be skipped. Once a library is prepped, library QC will also be performed using the Agilent Bioanalyzer to determine the library size and purity, and it also provides a Molarity value, though it is not always accurate. If no other downstream quantification method is available, this molarity value can be used for loading onto the sequencer. However, qPCR is highly recommended and is used to precisely quantify the library prior to loading the library onto the sequencer. Overloading or underloading can lead to failures in sequencing or poor quality data.

@keyleanardtabonda1431

3 жыл бұрын

@@abmgood thank you very much. New subscriber here 🙂

@azchannel4045

2 жыл бұрын

oke bosss ku...

It was totally helpful to me, Thanx!

@abmgood

4 жыл бұрын

Thanks for watching!

Happy learning. Thankyou 🙌

@abmgood

Жыл бұрын

Happy to hear that you learn something from our videos! It's our pleasure!

prior knowledge of the gene (of course) that answers the question I had. tyvm

@abmgood

4 жыл бұрын

Hi there, thanks for watching and yes do let us know if you have any questions about the material presented!

@nobodyspecial8127

4 жыл бұрын

@@abmgood Yes thank you very much. I am studying genomic medicine and a lot of the rocket scientist in my course are intimidating so this video is the most helpful video I have seen on rudimentary RNA-Seq and I will recommend it to co-students who are having trouble. My classes are RNA SARS Covid-19 I have a lot more confidence now.

Very helpful and told the subtle differences. I have a question can we actually perform the differential expression fo the genes between the cancer samples (example- subtype or stage etc) without comparing them to control(non-cancer samples)? I appreciate your input

@abmgood

3 жыл бұрын

Yes, during the bioinformatics analysis stage, you can set up your comparisons such that you are comparing cancer to cancer samples or cancer to control samples. I.e. You can choose which set of samples to use for the comparison.

@ChakravarthyGarlapati

3 жыл бұрын

@@abmgood sorry I didn't clearly mention the question in my previous message. To rid off the somatic mutations we use the non cancerous sample control. But can we do it with out performing this as do the differential analysis of cancer to cancer?

Excellent explanation

@abmgood

3 жыл бұрын

Thanks so much! Glad you found the video helpful.

very nice information for bigginers.

@abmgood

2 жыл бұрын

Glad you found the video helpful! Thanks for watching!

Is single cell sequencing the hottest spot in the current sequencing field？

Thanks alot

@abmgood

4 жыл бұрын

Thanks for watching!

Great!

@abmgood

3 жыл бұрын

Thanks for watching! :)

theng you.....boss

THX

@abmgood

4 жыл бұрын

You're welcome! Thanks for watching.

great

@abmgood

12 күн бұрын

Thanks!

data analysis 22:00