Пікірлер

• 
  @anivietv279Күн бұрын

  Tell me bout genomic technique at least there will work...

• 
  @bio366geethasankar714 күн бұрын

  🙏🏻

• 
  @SlavTiger14 күн бұрын

  Humans hijacking a virus hijacking a virus hijacking a human cell. Wow

• 
  @polinaskrobova563619 күн бұрын

  Hi! What secondary annealing means?

• 
  @faanutk786118 күн бұрын

  hairpin and selfdimer are secoundry annealing

• 
  @richard.f24 күн бұрын

  Very helpful! Greetings from Brazil.

• 
  @abmgood20 күн бұрын

  We're glad to hear that!

• 
  @juanete6928 күн бұрын

  Very good guide.

• 
  @abmgood20 күн бұрын

  We're glad to hear that!

• 
  @MohammadMahmoud-ji1jcАй бұрын

  I have created arms PCR primer; might I perhaps email it for revision?

• 
  @abmgood20 күн бұрын

  Yes of course! Please email your inquiry to [email protected], and our tech team will look into it! :D

• 
  @MohammadMahmoud-ji1jc19 күн бұрын

  @@abmgood thank you

• 
  @rahulbasu1681Ай бұрын

  PAM is located opposite strand compared to the strand where guide binds.

• 
  @only1slick420Ай бұрын

  HIGHLY UNRECOMMENDED

• 
  @rahulbasu1681Ай бұрын

  Fantastic, brief and awesome video, I was trying to understand the Crispr A system, it was so nicely explained here. Thanks a lot!

• 
  @abmgood20 күн бұрын

  We're glad that it helps!

• 
  @SmilingBoomBox-ro7gmАй бұрын

  ❤❤❤

• 
  @Sao733732 ай бұрын

  Such a cute little video with lots of information. Tell me what happens to the experiments? Where do these mature “cells” go? The CRISPR-Cas9 creations?

• 
  @abmgood20 күн бұрын

  We are glad that it helps! We will cryopreservation the cells after screening and QC. Please see this video for more about CRISPR-Cas9! kzread.info/dash/bejne/Y5V-2rqQm6iZdc4.html

• 
  @AGB_2 ай бұрын

  Pq no hay elado n la cafeteria de la USJ? kobhñp noug b

• 
  @AGB_2 ай бұрын

• 
  @Neeraj_91532 ай бұрын

  great

• 
  @abmgood20 күн бұрын

  Thanks!

• 
  @michaelcameron39022 ай бұрын

  I was wondering why you included the PGK-Puro within the AAVS homology arms? If the CMV promoter is constitutive then can't we just select based on red flourescence?

• 
  @michaelcameron39022 ай бұрын

  Also because this way our knock-in only contains the GOI. My understanding is that this is necessary because we wouldn't want to transfect other selectable markers if we were using this for something commercial, like in agriculture.

• 
  @user-jr9tx9sy1m3 ай бұрын

  This is the best I have seen so far on RNA-seq... Thank you so much, sensei.

• 
  @abmgood20 күн бұрын

  Glad it was helpful!

• 
  @mayling10143 ай бұрын

  Thank you for the wonderful visual+explanation! 15:53 May I know does the fragmentation occur before cDNA synthesis or after? I look through the protocol from Illumina they fragmented the RNA before cDNA synthesis

• 
  @tayyabanazim1003 ай бұрын

  ❤

• 
  @user-kk3xd6jr6z3 ай бұрын

  link error: 404 not found

• 
  @nanand293 ай бұрын

  Thank u❤

• 
  @abmgood20 күн бұрын

  You're welcome 😊

• 
  @jd39434 ай бұрын

  Even though this video was 8 years ago, it still seems great. I love it. Thanks a lot

• 
  @kiplimosimon14294 ай бұрын

  Excellent work. Thanks so much for sharing.

• 
  @bashkillszombies4 ай бұрын

  You left out the all important part everyone wants to know. The kit costs $360 USD.

• 
  @PatBythrow4 ай бұрын

  I made cell immortalization part of my morning routine and was able to cut my coffee consumption almost in half!

• 
  @mikebenstead76004 ай бұрын

  Who, if anyone, monitors and regulates CRISPR usage? What's to stop this tech falling into the wrong hands?

• 
  @adnanwali72724 ай бұрын

  Amazing video thanks.. please reduce the speed of audio.

• 
  @habibanazeera4 ай бұрын

  The background music is a noise.

• 
  @JYOtiRaNJanMANgaRaj4 ай бұрын

  ❤❤❤❤❤❤

• 
  @soniakhan94724 ай бұрын

  Can this technique be used to generate knock-in mice?

• 
  @yogeshnepal56434 ай бұрын

  What is the use of ColE1? does that the origin of replication for the replication of plasmid inside the mammalian cells?

• 
  @soniakhan94725 ай бұрын

  A brilliant, clear and comprehensive video!

• 
  @alhaeri15 ай бұрын

  fantastic video

• 
  @quangvinhbui77335 ай бұрын

  Hello, Can you answer this question that if non-engineered lentivirus don't have psi gene for packing then what gene they use to do that ?

• 
  @kiedranFan20356 ай бұрын

  Additionally the repression/activation protiens can be engineered to bind to an extention of the guide RNA's so that multiple reactions can take place simultainously. The dCas9 shown here would only be good for one reaction at a time, where all genes targets are activated or repressed but cant do both for different sets of gene targets. RNA binding components are able to do that though and is also an option.

• 
  @JasonLubega6 ай бұрын

  Very well explained introduction to Adenoviruses as vectors.

• 
  @sam2theammyk96 ай бұрын

  Micromolar filter 😂

• 
  @user-jc2we4sn1i6 ай бұрын

  I taught a neighbor boy how to make an electroporator from an AM FM radio powered by 9 volt batteries.

• 
  @abumohammed96597 ай бұрын

  that is great thank you!

• 
  @nurulainafandi42677 ай бұрын

  What is meant by Tm of product?...what is meant by word product?????

• 
  @alfomeg5887 ай бұрын

  melting temperature

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  @akinyemikindnessoluwole33527 ай бұрын

  THis is my first clear understanding to PCR Am very impressed Thanks a bunch

• 
  @MahdiZia.razzaz8 ай бұрын

  very explanatory, thank you

• 
  @ramakrazavi63148 ай бұрын

  Thank you so much for clarifying such a hard topic in a simple language. It was very helpful.

• 
  @abmgood8 ай бұрын

  Glad it was helpful!

• 
  @chitraanbu64309 ай бұрын

  Tq so much for this informative video

• 
  @rayzhang9289 ай бұрын

  abm u r the goat for making these videos. thank u

• 
  @immylon46089 ай бұрын

  Nice explanation. Is it safe to consider Infection unit in this video is same as PFU(plaque forming units)?

• 
  @AmruMagdy10 ай бұрын

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  @user-jp9pr6qk9e10 ай бұрын

  ❤

• 
  @Mukesh.Behera10 ай бұрын

  Great explanation ❤

• 
  @AliButt-ve1jr10 ай бұрын

  😊