Technique Talk: The Basics of Immunohistochemistry

Ғылым және технология

This Technique Talk will explore the science behind IHC technology and highlight how it has developed over time. Steven Hrycaj will talk about protocol development, optimization, and troubleshooting, and how sample properties impact protocol decisions. Finally, he will discuss how automation technology has changed IHC, comparing and contrasting automated and manual in situ hybridization protocols and applications.

Пікірлер: 11

  • @thymusictoo364
    @thymusictoo364 Жыл бұрын

    Thank you very much to everyone involved with this and making it publicly available! it's helped me very much in learning how to do IHC 🌻

  • @sinobiologicalinc.5415

    @sinobiologicalinc.5415

    Жыл бұрын

    Great to hear, I'm glad you enjoyed!!

  • @talashpeygir2621
    @talashpeygir2621 Жыл бұрын

    thank you for excellent new information

  • @francescolandini7947
    @francescolandini7947 Жыл бұрын

    Nice presentation! Very Educational! Thanks 🙏

  • @sinobiologicalinc.5415

    @sinobiologicalinc.5415

    Жыл бұрын

    Glad it was helpful!

  • @cyberrosa4720
    @cyberrosa472011 ай бұрын

    Great talk! I can use clarification as to which steps to take towards the embedded block for under or over fixation of the tissue? Is there a protocol for these two scenarios?

  • @SarkarSAziz
    @SarkarSAziz Жыл бұрын

    Hi Sir. Thanks for the informative presentation. I got a question, please. How do antibodies enter the cells? I need an exact answer. Is it because of: 1- mechanical destruction of the cells during microtomy? 2- Using ethanol and xylene? during the tissue preparartion?

  • @SarkarSAziz

    @SarkarSAziz

    Жыл бұрын

    @@jlmjkooj thanks a lot for your answer.

  • @user-zd3km9dr5h
    @user-zd3km9dr5h2 жыл бұрын

    Can you provide the ppt?

  • @bhaskardesai4106
    @bhaskardesai41067 ай бұрын

    Is there any research going on for elimating or stop production of Aquaporin 4 Antibody in spinal Cerebral water channel, a main route cause of Neurological disorder disease, NMOSD. IF possible, kindly give details of present condition in research for NMOSD.

  • @francescolandini7947
    @francescolandini7947 Жыл бұрын

    Hier Pier EPITOPE retrieval! Not Enzyme.. error in the slides

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