Excellent videos! Short and sweet with appropriate visuals to help understanding. Please do more of these! :D

What a brilliant lecture. Thank you.

Very good Job explaining! This should be higher up in the search list.

Again..your videos are just amazingly explained so clearly

@Microcourses

3 жыл бұрын

Thank you so much 😀

Another top quality vid.

say you're using a 60X objective with a wide field, would binning decrease the resolution? Pixel size w/o binning is probably going to be in the neighborhood of 125nm or so depending on the configuration, and binned pixels will be at least double that, what do you recommend?

@Microcourses

4 жыл бұрын

Optimal pixel size depends on the numerical aperture of the objective lens (in addition to magnification - there is a microcourse on numerical aperture if you aren't familiar with it) and what you are trying to image (ie, diffraction-limited objects would require a different pixel size than whole cells), so there isn't a standard recommendation. I recommend you post the question on our microscopy discussion forum at forum.microlist.org - it's quite active so you should get some answers there!

Thank you for your videos

awesome, thank you :]

Thank you

This was great! Could you please post a video on Fourier transform?

@Microcourses

4 жыл бұрын

Thanks! It's on the list of future videos. In the meantime, Bo Huang's video in the iBiology microscopy lecture series is very good.

@shivalidongre4123

4 жыл бұрын

@@Microcourses thank you! I will check it out

The curve at 1:30 under noise was used in today's online workshop over Zoom!

@Microcourses

4 жыл бұрын

Good eye Tao! Talley made those slides, and we all use them. :)

🦉

increase the subject illumination!!!

@Microcourses

7 ай бұрын

Sure, with the trade-off of increased photobleaching, and phototoxicity in live samples.