Sandwich ELISA assay

This lecture explains about the Sandwich ELISA assay technique.
Sandwich ELISA is a much less customary variant of ELISA, however is highly effective in pattern antigen detection. Additionally, many business ELISA pair sets are built on this sandwich ELISA.
This video lecture explains the mechanism of Sandwitch elisa assay to detect the presence of antigen trapped inside two layers of antibodies.
For more information, log on to-
www.shomusbiology.com/
Get Shomu's Biology DVD set here-
www.shomusbiology.com/dvd-store/
Download the study materials here-
shomusbiology.com/bio-material...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in KZread. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.com/1136485849827...
LinkedIn - / suman-bhattacharjee-2a...
KZread- / thefunsuman
Thank you for watching the video lecture on Sandwich ELISA assay mechanism and their role in detecting antigen in patient serum.

Пікірлер: 93

  • @dharanik.biotechnologyldc9034
    @dharanik.biotechnologyldc90347 жыл бұрын

    very usefully and now i have got the clear cut idea abtout the sandwich eliza .tx sir u have done a gd job

  • @nwilczyn
    @nwilczyn7 жыл бұрын

    all this talk of sandwich ELISA is making me glad it is almost lunch time

  • @MdMahmud-vd9ck
    @MdMahmud-vd9ck6 жыл бұрын

    You are great teacher ! Keep doing your good work !

  • @ahmadalsalim9630
    @ahmadalsalim96307 жыл бұрын

    thanks, man very clear explanation

  • @sheikhrayees8678
    @sheikhrayees86786 жыл бұрын

    Thanx sir u make every technique easier to understand keep going and best of luck

  • @nehanaranje8134
    @nehanaranje81346 жыл бұрын

    thank you it was very helpful

  • @BalaBiologyWorld
    @BalaBiologyWorld8 жыл бұрын

    wow fantastic.....

  • @hasaankhan1048
    @hasaankhan10488 жыл бұрын

    Could you talk a little bit about a cell-based assay (CBA)? Much appreciated.

  • @kashishsahil3546
    @kashishsahil35466 жыл бұрын

    really good presentation

  • @maryanncrystaluzodinma2391
    @maryanncrystaluzodinma23918 жыл бұрын

    good job sir...

  • @BIBECHYOUTUification
    @BIBECHYOUTUification4 жыл бұрын

    Shomu's Biology is great source of knowledge. Thank you for this information and keep educating us.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

  • @alrobai
    @alrobai8 жыл бұрын

    l looked over all your videos and I didn't find video about pSico nor Lentiviral technologies. Would you pleeeeeese do a lecture about pSico and using Lentiviral vector technology to deliver the gene of interest?? I couldn't understand these two technology.

  • @gayatrideepti6220
    @gayatrideepti62203 жыл бұрын

    Hello! Very nice lecture 👍. I have a question about ELISA. Is it possible to add the secondary antibody first and the enzyme in the next step in the procedure without combining them both and adding it to the sample . If so, would there be any difference?

  • @messedupkeys8457
    @messedupkeys84576 жыл бұрын

    Does the SD HIV1/2 test kit has the same principle as the sandwich EIA?

  • @tamannamalik6931
    @tamannamalik69316 жыл бұрын

    Thank you sir.

  • @BinhPhan-wn4dj
    @BinhPhan-wn4dj4 жыл бұрын

    Thank you for your information. Could you talk more about how to the first Ab can capture on assay plate? What is characterization of assay plate for Sandwich ELISA?

  • @muthyalakapil8743
    @muthyalakapil87434 жыл бұрын

    Sir what is the substrate we have add in this reaction.please clarify my doubt sir

  • @urvashibhatara3626
    @urvashibhatara36267 жыл бұрын

    It was very informative. I have watched your videos on RIA, Competitive ELISA also and liked them. Thank you Sir.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Urvashi Bhatara thank you. Glad you liked my lectures

  • @globalbiologicalacademy2784
    @globalbiologicalacademy27848 жыл бұрын

    Would you please upload a video about Oxidative stress?

  • @nawazishaliqazalbash907
    @nawazishaliqazalbash9077 жыл бұрын

    please explain what is Enzyme-linked fluorescent assay (ELFA)?

  • @jonathanbensonmugaka7608
    @jonathanbensonmugaka76084 жыл бұрын

    I like the video,it makes me understand all types of ELISA easily

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @yonasgebrehiwot2260
    @yonasgebrehiwot22606 жыл бұрын

    GOD BLESS U

  • @reshmasamanta4845
    @reshmasamanta48457 жыл бұрын

    WOW .............NYC SIR.................

  • @Reni8705
    @Reni87056 жыл бұрын

    Thank you so much 🤓🤓🤓

  • @doctoralaaal-wattar2127
    @doctoralaaal-wattar21277 жыл бұрын

    THANKS DEAR

  • @districthospitaltumakuru1556
    @districthospitaltumakuru15564 жыл бұрын

    excellent lecture, very simple and easy to understand, thank you so much

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @bbe3331
    @bbe33314 жыл бұрын

    Thank you for your wonderful and informative lectures.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @isadoras8686
    @isadoras86867 жыл бұрын

    Your videos help me get through my genetics degree, so straight forward and well explained :)

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Isadora Diamond thank you. It's a pleasure to hear that.

  • @khurramkhan7313

    @khurramkhan7313

    6 жыл бұрын

    i love u

  • @himansunayak3917
    @himansunayak39177 жыл бұрын

    sir plz can u send me d link of ELISA???

  • @ankitachoudhury5546
    @ankitachoudhury55463 жыл бұрын

    Is the antibody Z1 and Z2 raised in different species?

  • @meghnajain1576
    @meghnajain15764 жыл бұрын

    You are running an ELISA which uses the polyclonal secondary antibody . What type of ELISA it is? What could be the major issues that can arise due to the use of polyclonal antibody? How will you reduce the background noise in such case?

  • @ymmalladad2663
    @ymmalladad26635 жыл бұрын

    It's Very helpful Sir thank you so much sir ☺☺

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad to hear that you are getting benefit from the videos

  • @yasmeenzia7844
    @yasmeenzia78446 жыл бұрын

    ELISA ka lecture kaha hy mujay nhi mil raha ....

  • @ImranAhmad-df3mi
    @ImranAhmad-df3mi5 жыл бұрын

    Always osm sir nyc explanation as usual thanks a lot Sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Glad to help you out

  • @learntogether8900
    @learntogether89005 жыл бұрын

    Thank You Sir. It's very helpful for my exam.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @ugurboralfan1
    @ugurboralfan14 жыл бұрын

    thank u sir

  • @anshumehta8222
    @anshumehta82224 жыл бұрын

    thank you for this explanation helped a lot

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @sakshichandra7777
    @sakshichandra77773 жыл бұрын

    It's very helpful😊..and easy to understand thank you so much..🙏🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @dhanrajpaulp6678
    @dhanrajpaulp66782 жыл бұрын

    Shomu I love ur way of teaching 💋....

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Thank you so much for appreciating my efforts

  • @durgeshsingh5031
    @durgeshsingh50312 жыл бұрын

    Sir acc. To kuby only enzyme-linked antibody specific for different epitope is added nd thn substrate is added to measure the colour. But u said we add primary antibody after that another secondary antibody.

  • @pieter2160
    @pieter21603 жыл бұрын

    Great explanation!

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @ErtugrulGhazi-mv8yq
    @ErtugrulGhazi-mv8yq4 жыл бұрын

    bht help hori hay bhai shukrya

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    You're welcome

  • @akshayavidhya9325
    @akshayavidhya93257 жыл бұрын

    thank you sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    7 жыл бұрын

    +Akshaya Vidhya you're welcome

  • @anilbudumuru3969
    @anilbudumuru39694 жыл бұрын

    Sir Elisa human body Loki ela enter avutuendi plz reply Sir

  • @soumighosh9075
    @soumighosh90753 жыл бұрын

    Why it's more sensitive?

  • @twiringiyimanaemmanuel9742
    @twiringiyimanaemmanuel97425 жыл бұрын

    nice work . Shomu.

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    Thank you

  • @sangeethatalks8250
    @sangeethatalks82502 жыл бұрын

    Thank you sir♥️

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    You're welcome

  • @sadiamumtaz5057
    @sadiamumtaz50578 жыл бұрын

    great sir g, how can v save these videos for offline listening.....

  • @paramjitKaur-qx3bi

    @paramjitKaur-qx3bi

    4 жыл бұрын

    Download it.

  • @komuravellyvamshikrishna8953
    @komuravellyvamshikrishna89533 жыл бұрын

    Tq fir good explaination sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    Thank you

  • @Fry942
    @Fry942 Жыл бұрын

    clear explanation

  • @shomusbiologyofficial

    @shomusbiologyofficial

    Жыл бұрын

    Thank you

  • @Parul14315
    @Parul143153 жыл бұрын

    Thank you sir 😊

  • @shomusbiologyofficial

    @shomusbiologyofficial

    3 жыл бұрын

    You're welcome. Glad to hear that you're getting benefit from my lectures

  • @linadhote7195
    @linadhote71954 жыл бұрын

    Very good sir...

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Thank you

  • @kaushiksen281
    @kaushiksen2814 жыл бұрын

    It would be really helpful if u can kindly give us an insight about 'Double Antigen Sandwich Elisa'

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    Okay

  • @dr.lakshmisruthi3392
    @dr.lakshmisruthi33927 жыл бұрын

    pls improve audio clarity.

  • @alkakumari3872
    @alkakumari38725 жыл бұрын

    Thanku sir

  • @shomusbiologyofficial

    @shomusbiologyofficial

    5 жыл бұрын

    You're welcome

  • @umamishra1407
    @umamishra14074 жыл бұрын

    Sir why we need two antibodies we can also put only one enzym linked antibody plzzz reply.............waiting eagerly for your answer to clear my doubt🙏🙏🙏🙏🙏

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    That can cause u wanted binding and background noise.

  • @debarshinipoddar7493
    @debarshinipoddar74932 жыл бұрын

    Can you please share where after Masters in Medical Physiology can a person apply for job

  • @shomusbiologyofficial

    @shomusbiologyofficial

    2 жыл бұрын

    Yes you can apply for job in diagnostics

  • @RitikaVishnoi9
    @RitikaVishnoi94 жыл бұрын

    Why can't we use same antibodiy as the antigen is same??????

  • @anchlakumari7704
    @anchlakumari77047 жыл бұрын

    please send me video from kuby

  • @ErtugrulGhazi-mv8yq
    @ErtugrulGhazi-mv8yq4 жыл бұрын

    sir i want cmplt video

  • @shomusbiologyofficial

    @shomusbiologyofficial

    4 жыл бұрын

    U

  • @chandanaraghuraj188
    @chandanaraghuraj1883 жыл бұрын

    👍

  • @rahishaaz4392
    @rahishaaz43925 жыл бұрын

    Sir...strip based hiv test is which type of ELISA???? Plzzz need answer...

  • @sudhabalraj9586
    @sudhabalraj95863 жыл бұрын

    7th day, 10th concept!

  • @debabratadasbarman5283
    @debabratadasbarman52833 жыл бұрын

    Sound is very bad