Sandwich ELISA assay
This lecture explains about the Sandwich ELISA assay technique.
Sandwich ELISA is a much less customary variant of ELISA, however is highly effective in pattern antigen detection. Additionally, many business ELISA pair sets are built on this sandwich ELISA.
This video lecture explains the mechanism of Sandwitch elisa assay to detect the presence of antigen trapped inside two layers of antibodies.
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Thank you for watching the video lecture on Sandwich ELISA assay mechanism and their role in detecting antigen in patient serum.
Пікірлер: 93
very usefully and now i have got the clear cut idea abtout the sandwich eliza .tx sir u have done a gd job
all this talk of sandwich ELISA is making me glad it is almost lunch time
You are great teacher ! Keep doing your good work !
thanks, man very clear explanation
Thanx sir u make every technique easier to understand keep going and best of luck
thank you it was very helpful
wow fantastic.....
Could you talk a little bit about a cell-based assay (CBA)? Much appreciated.
really good presentation
good job sir...
Shomu's Biology is great source of knowledge. Thank you for this information and keep educating us.
@shomusbiologyofficial
4 жыл бұрын
Thank you
l looked over all your videos and I didn't find video about pSico nor Lentiviral technologies. Would you pleeeeeese do a lecture about pSico and using Lentiviral vector technology to deliver the gene of interest?? I couldn't understand these two technology.
Hello! Very nice lecture 👍. I have a question about ELISA. Is it possible to add the secondary antibody first and the enzyme in the next step in the procedure without combining them both and adding it to the sample . If so, would there be any difference?
Does the SD HIV1/2 test kit has the same principle as the sandwich EIA?
Thank you sir.
Thank you for your information. Could you talk more about how to the first Ab can capture on assay plate? What is characterization of assay plate for Sandwich ELISA?
Sir what is the substrate we have add in this reaction.please clarify my doubt sir
It was very informative. I have watched your videos on RIA, Competitive ELISA also and liked them. Thank you Sir.
@shomusbiologyofficial
7 жыл бұрын
+Urvashi Bhatara thank you. Glad you liked my lectures
Would you please upload a video about Oxidative stress?
please explain what is Enzyme-linked fluorescent assay (ELFA)?
I like the video,it makes me understand all types of ELISA easily
@shomusbiologyofficial
4 жыл бұрын
You're welcome
GOD BLESS U
WOW .............NYC SIR.................
Thank you so much 🤓🤓🤓
THANKS DEAR
excellent lecture, very simple and easy to understand, thank you so much
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
Thank you for your wonderful and informative lectures.
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
Your videos help me get through my genetics degree, so straight forward and well explained :)
@shomusbiologyofficial
7 жыл бұрын
+Isadora Diamond thank you. It's a pleasure to hear that.
@khurramkhan7313
6 жыл бұрын
i love u
sir plz can u send me d link of ELISA???
Is the antibody Z1 and Z2 raised in different species?
You are running an ELISA which uses the polyclonal secondary antibody . What type of ELISA it is? What could be the major issues that can arise due to the use of polyclonal antibody? How will you reduce the background noise in such case?
It's Very helpful Sir thank you so much sir ☺☺
@shomusbiologyofficial
5 жыл бұрын
Glad to hear that you are getting benefit from the videos
ELISA ka lecture kaha hy mujay nhi mil raha ....
Always osm sir nyc explanation as usual thanks a lot Sir
@shomusbiologyofficial
5 жыл бұрын
Glad to help you out
Thank You Sir. It's very helpful for my exam.
@shomusbiologyofficial
5 жыл бұрын
You're welcome
thank u sir
thank you for this explanation helped a lot
@shomusbiologyofficial
4 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
It's very helpful😊..and easy to understand thank you so much..🙏🙏
@shomusbiologyofficial
3 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
Shomu I love ur way of teaching 💋....
@shomusbiologyofficial
2 жыл бұрын
Thank you so much for appreciating my efforts
Sir acc. To kuby only enzyme-linked antibody specific for different epitope is added nd thn substrate is added to measure the colour. But u said we add primary antibody after that another secondary antibody.
Great explanation!
@shomusbiologyofficial
3 жыл бұрын
Thank you
bht help hori hay bhai shukrya
@shomusbiologyofficial
4 жыл бұрын
You're welcome
thank you sir
@shomusbiologyofficial
7 жыл бұрын
+Akshaya Vidhya you're welcome
Sir Elisa human body Loki ela enter avutuendi plz reply Sir
Why it's more sensitive?
nice work . Shomu.
@shomusbiologyofficial
5 жыл бұрын
Thank you
Thank you sir♥️
@shomusbiologyofficial
2 жыл бұрын
You're welcome
great sir g, how can v save these videos for offline listening.....
@paramjitKaur-qx3bi
4 жыл бұрын
Download it.
Tq fir good explaination sir
@shomusbiologyofficial
3 жыл бұрын
Thank you
clear explanation
@shomusbiologyofficial
Жыл бұрын
Thank you
Thank you sir 😊
@shomusbiologyofficial
3 жыл бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
Very good sir...
@shomusbiologyofficial
4 жыл бұрын
Thank you
It would be really helpful if u can kindly give us an insight about 'Double Antigen Sandwich Elisa'
@shomusbiologyofficial
4 жыл бұрын
Okay
pls improve audio clarity.
Thanku sir
@shomusbiologyofficial
5 жыл бұрын
You're welcome
Sir why we need two antibodies we can also put only one enzym linked antibody plzzz reply.............waiting eagerly for your answer to clear my doubt🙏🙏🙏🙏🙏
@shomusbiologyofficial
4 жыл бұрын
That can cause u wanted binding and background noise.
Can you please share where after Masters in Medical Physiology can a person apply for job
@shomusbiologyofficial
2 жыл бұрын
Yes you can apply for job in diagnostics
Why can't we use same antibodiy as the antigen is same??????
please send me video from kuby
sir i want cmplt video
@shomusbiologyofficial
4 жыл бұрын
U
👍
Sir...strip based hiv test is which type of ELISA???? Plzzz need answer...
7th day, 10th concept!
Sound is very bad