Sample Preparation by Ashing Method for the Analysis of Heavy Metals & Minerals Using AAS
Sample preparation is very crucial for the analysis of heavy metals & minerals using Atomic Absorption Spectrometer. This video represents a details procedure for the sample preparation of heavy metals & minerals analysis.
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Determination of Ash: • Determination of Ash C...
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Пікірлер: 127
Very much practical oriented. Nice 🎉
Which method dry ashing or wet digestion is suitable for determining minerals in liquid supplements???
Can I use this method to detect heavy metals from cosmetics products like eyeshadow...or should I use wet digestion method
So satisfying to watch.
@MicroChemsExperiments
Жыл бұрын
Thank you
2:03 did u soak the crucible in nitric acid for 12 hours to make sure there's no other component left in there? used deionized water to wash and remove the acid before using? thank you
@MicroChemsExperiments
Жыл бұрын
Yes. You are correct.
I want to see further steps for heavy metal analysis (how to determine quantity of any metal pb/cr/zn)...do you have any video on this??
Hai want to ask, is it necessary to use filter paper Whatman 42 ? because in my lab just have qualitative filter paper such as Ross 1a, Whatman 1PS, Munktell 1F and MGC..
@MicroChemsExperiments
3 жыл бұрын
Yes you can use any of these of smaller pore size among your mentioned filter papers.
9:05 why is there a need to dilute the ash with 1N nitric acid? may we use other acid for this? thank you very much!
@MicroChemsExperiments
Жыл бұрын
Nitric Acid solution is best for heavy metal and mineral analysis
Why is it necessary to grind the sample into powdery form before ashing?
Hello! I just wanted to ask - what is the purpose of the filtering step? What is being filtered out? Thank you!
@MicroChemsExperiments
2 жыл бұрын
Filtration is done to ensure that any sort of interference will be filtered out, if any, present in the Ash. Ash particles, which are not dissolved in the diluent, may be filtered out.
Can this method be used for determining heavy metals in plants? from leaves, roots, and stems?
@MicroChemsExperiments
2 жыл бұрын
Yes. You can use
I plan to measure ash content and then measure mineral content and also heavy metals. For heavy metals i would like to use wet digestion method. In your opinion, do i need to make a wet digestion if i already drying ash from measuring ash content before? Thanks in advance!
@MicroChemsExperiments
10 ай бұрын
Yes. For heavy metal analysis, wet digestion is better method than the dry ashing.
@nurulainnajwasuhaimai4418
10 ай бұрын
@@MicroChemsExperiments okay thank youu btw your videos really helps me 👍🏻
If I do dry ashing before and use AAS next day, can I do like that? And what should I do? Because i don't have time enough for it. Thanks for answering
@MicroChemsExperiments
Жыл бұрын
Keep the Ash sample closed to avoid contamination and prepare the sample from ash on the day of analysis in AAS. Or you can keep and store the prepared solution at 4-8 degree Celsius.
Can we used this method for the determination of heavy metals in water (waste, naturel) ? if yes, wath is the volume used for sampling? Thank you
@MicroChemsExperiments
2 жыл бұрын
Yes. You can. You can use 2ml of sample
In determining heavy metal metals in tea samples , can this method be used ?
@MicroChemsExperiments
2 жыл бұрын
Yes. You can use.
I want to analyze some elements in ash salt (filtrate). The ash was already got from burnt plant residue and the ash salt was prepared by leeching water through the ash material. Is it possible to analyze the already prepared ash salt. It is already in solution.
@MicroChemsExperiments
Жыл бұрын
If the ash salt is prepared correctly (complete ash with no black color) then you can test your sample with this solution
Great work😊
@MicroChemsExperiments
3 ай бұрын
Thank you
can i use this method to determine Calcium, Iron, Copper, Manganese, Zinc, Sodium and Potassium in onion?
@MicroChemsExperiments
Жыл бұрын
Yes you can
Can i use that method for cerels-wheat and maize,and sunflower meal.And any huge loss of mercury with this or not?
@MicroChemsExperiments
Жыл бұрын
You can use this method
why do you use a pen marker? is required special marker stell pin, the pin that uses is no effect disappear in a muffle furnace?
@MicroChemsExperiments
3 жыл бұрын
You are absolutely right. Stell pin was not available to us at that moment, thats why we have shown by pen marker.
Magnificent
@MicroChemsExperiments
3 жыл бұрын
@Mohammad Shahdat Hossain Thank you sir😊😊
can i use UV-visible spectrophotometer instead of AAS
@MicroChemsExperiments
2 жыл бұрын
If you have method for UV then you can prepare your sample by this method.
Can this method be used for determining Lead (Pb) in lead sulfide ores and lead contentrates.
@MicroChemsExperiments
2 жыл бұрын
Yes.
@Chemtoliify
2 жыл бұрын
@@MicroChemsExperiments thank you so much, please keep going
what is the volume of volumetric flask that you use in filtration sample process ? Is it 50ml ?
@MicroChemsExperiments
3 жыл бұрын
You are right. 50ml
@syahirahatiqah9459
3 жыл бұрын
thank you for the reply :)
Kindly mention the maximum temperature and hour. Thank you for your reply
@MicroChemsExperiments
2 жыл бұрын
Max temperature is 650 degree celcius and time is 7 hours.
Can I store the prepared sample in refrigerator and at what temperature? I want to send the sample to another place because my laboratory don't have AAS. Thank you
@MicroChemsExperiments
Жыл бұрын
Store at 4-8 degree Celsius and maintain that temperature during transportation
Can I replace the deionized water with distilled water (aquades)? Thank you
@MicroChemsExperiments
Жыл бұрын
No
Can we use ashing to determine pb ,cu, cd, cromium& mercury in yogurt?
@MicroChemsExperiments
2 жыл бұрын
Yes. You can use this method.
@turkcearapca4297
2 жыл бұрын
Ok, Thanks for your answer❤❤
Hi. May i know this method suitable for Calcium and Phosphorus? Or wet digestion will ne better.??
@teddyku6159
2 жыл бұрын
Be*
@MicroChemsExperiments
2 жыл бұрын
Wet Digestion
hi, can i use this method for determination of arsenic in rice?
@MicroChemsExperiments
Жыл бұрын
Thank to you too
Do I use microwave oven for sample dry? It's possible?
@MicroChemsExperiments
2 жыл бұрын
Yes. You can use Microwave Oven for drying the sample.
can we use this method for calcium, phosphorous, mg, mn, iodine, sodium and iron , if yes can we this method by using HCL
@MicroChemsExperiments
Жыл бұрын
Yes
@foodotechos7600
Жыл бұрын
@@MicroChemsExperiments and with hcl also?
can this method be use to determine the ammount of heavy metal in shrimp
@MicroChemsExperiments
2 жыл бұрын
Yes, you can use
Can this method used for liquid sample in cosmetic sample for example??
@MicroChemsExperiments
Жыл бұрын
Yes. You can
Can we used for PVC VN-65L 1 and PP materials?
@MicroChemsExperiments
11 ай бұрын
Not sure about it
I want to prepare a sample from mango because I need details of the ash content so please give me suggestions about how to prepare a dry sample from mango ie. From fruit.
@MicroChemsExperiments
2 жыл бұрын
1. Remove moisture by following this video: kzread.info/dash/bejne/dqiHqpqnYc3IqbA.html You will get a dry sample. 2. Determine ash content by following this video: kzread.info/dash/bejne/oGaF2cRxgNqeiag.html
in 7:05, if the color still black, does it mean the sample still contain organic matter? is that why we should add some acid?
@MicroChemsExperiments
3 жыл бұрын
You are right. Presence of Black color means that the sample still contains organic matters. At this point we add some acid. This addition of acid facilitates to break down the Carbon strong bonds making free carbon which can easily be removed from the sample by applying additional heat.
@shafadaraillona7850
3 жыл бұрын
@@MicroChemsExperiments thank u so much!!
@MicroChemsExperiments
3 жыл бұрын
@@shafadaraillona7850 most welcome
What's the different between this Ash method and Total Digestion method?
@MicroChemsExperiments
9 ай бұрын
No idea about total digestion method
Can we use the crucible without cover or lid
@MicroChemsExperiments
2 жыл бұрын
You can but you shouldn't as it may lead to the cross contamination
How i use liquid solution and how it filtered
@MicroChemsExperiments
3 ай бұрын
Your question is not cleared
Can this method be used for determining micronutrients in seeds? Plz reply
@MicroChemsExperiments
2 жыл бұрын
Only for heavy metal and mineral determination
@Shashikumar55822
2 жыл бұрын
@@MicroChemsExperiments kindly, tell the process used for the micronutrient analysis in seed? Plz reply
Can this method be used for determining heavy metals in blood serum?
@MicroChemsExperiments
Жыл бұрын
No. use the following method: kzread.info/dash/bejne/qHV528ern7DMh84.html
Kindly tell the process used to determine the micronutrient content in seeds. Plzz
@MicroChemsExperiments
2 жыл бұрын
kzread.info/dash/bejne/qHV528ern7DMh84.html
Sir , what is the need of blank sample in there? please explain me
@MicroChemsExperiments
2 жыл бұрын
Blank is needed to minimise the reading of reagent used for this preparation
Please 🥺 I want information a bout prepar blood serum to assess zinc and selenium by GF AAS
@MicroChemsExperiments
3 жыл бұрын
Karntoka Ali, you can analysis your serum sample for Zinc & Selenium by following our video of WET DIGESTION. No modification is needed
Can you please share the reference of this method .... Thanking in anticipation
@MicroChemsExperiments
2 жыл бұрын
Combined from AOAC method and journals and validated in-house.
Which method did you follow ?
@MicroChemsExperiments
3 жыл бұрын
Taha bazine, we followed ASEAN Manual For Food Analysis
@tahabazine3397
3 жыл бұрын
@@MicroChemsExperiments Thnx 😁
@MicroChemsExperiments
3 жыл бұрын
@@tahabazine3397 You are most welcome
Dnt need to heat with hno3
@MicroChemsExperiments
3 жыл бұрын
Kathuri Kk, No, Ashing Method dont need any heat with HNO3
Can you please share the CRM with me Thanks
@MicroChemsExperiments
2 жыл бұрын
How can I share?
I am asking normal microwave oven ( in home)
@MicroChemsExperiments
2 жыл бұрын
What did you mean by drying? You must need muffled furnace to make ash.
Aapki lab Kha h, Mugha seed oil ki iodine value, and some other properties nikalwani h
@MicroChemsExperiments
3 жыл бұрын
Kalkata main hamara lab hain. Iodine value determination method is already uploaded here. Same mathod should be followed for Mugha seed oil. Thank you.
@nitinyadav5004
3 жыл бұрын
Protein content in oil bhi daal do
@MicroChemsExperiments
3 жыл бұрын
@@nitinyadav5004 Detetmonation of ptotein is already uploaded. Pkease follow that method
@nitinyadav5004
3 жыл бұрын
I want to work in your lab
Don't waste water, you can close the tap while rubbing the crucible pot.
@MicroChemsExperiments
11 ай бұрын
You are correct.
@drabhijeetpachpor5498
11 ай бұрын
Thank you for your response, your videos are detailed and informative. Keep posting
@MicroChemsExperiments
11 ай бұрын
@@drabhijeetpachpor5498 Sure. Stay with us
Can this method be used for the determination of heavy metals in insects?
@MicroChemsExperiments
2 жыл бұрын
Yes. You can use
@jones547
2 жыл бұрын
@@MicroChemsExperiments what about the wet digestion method? Can we use it for insects heavy metal determination?
@MicroChemsExperiments
2 жыл бұрын
@@jones547 Yes. You can also use wet digestion method for insects
@jones547
2 жыл бұрын
@@MicroChemsExperiments Thank you so much 🙏 your video is really helpful for my project 😊
@MicroChemsExperiments
2 жыл бұрын
@@jones547 Thank you
what is the sample?
@MicroChemsExperiments
3 жыл бұрын
Animal Feed sample
@thesupermysteries1355
3 жыл бұрын
@@MicroChemsExperiments ok 😉😉
Please tell filter paper no.
@MicroChemsExperiments
2 жыл бұрын
Whatman Filter Paper-1