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Protocol 8: Miniprep (QIAprep Spin Kit)

This tutorial explains how to carry out a miniprep using the QIAprep® Spin Miniprep Kit provided by Qiagen®.
References:
QIAprep Miniprep Handbook
www.qiagen.com...

Пікірлер: 13

  • @adityagodara4426
    @adityagodara44267 жыл бұрын

    thank you guys for this video. i am in my last week of semester and was doing mini prep for my project. was so confused about this process but this video saved me. :) :) PS. you deserve extra points for telling why a certain buffer is added every time.

  • @kalkidanberhanu7708
    @kalkidanberhanu77082 жыл бұрын

    Thanks for the great overview! I am very new to molecular biology techniques so this makes me feel more comfortable :)

  • @My5t13
    @My5t137 жыл бұрын

    Thanks so much for this Video . It was such a big help. Had the same experience as Aditya. Now I can feel good about completing the report.

  • @handyhardyman
    @handyhardyman2 жыл бұрын

    Thanks! This video is very useful to learn how to use miniprep!!!

  • @judybrown2864
    @judybrown28644 жыл бұрын

    Note your centrifuge tubes should be lined up so that the hinges are all on the outside...this makes the pellet go to a known location I.e. the back of the tube...label your columns as well in case these get separated from the other tubes...otherwise well done

  • @bardiakhosravi7364
    @bardiakhosravi7364 Жыл бұрын

    very clear instructions, and very informative of the reasons behind each step! Thank you! If I had to add one thing, could you edit it so that you show the procedure if the vacuum manifold is used to wash the spin columns instead of centrifuging?

  • @gus..611
    @gus..6116 ай бұрын

    Quite useful! Thanks!

  • @FootyShorts510
    @FootyShorts5103 жыл бұрын

    thank you! i have a question though, what is the function of potassium acetate during the lysis phase?

  • @dylanjainchill4165
    @dylanjainchill41654 жыл бұрын

    Great video!

  • @annierichards7367
    @annierichards73675 жыл бұрын

    Thank you for your video.

  • @freshtasadat8242
    @freshtasadat82424 жыл бұрын

    hello, may i know the price of this kit please ?

  • @melissahahn8552

    @melissahahn8552

    2 жыл бұрын

    around 1.500 Euro

  • @best0731
    @best07318 жыл бұрын

    kk