Prokaryotic Translation INITIATION - How do 70S ribosomes initiate translation? - Deep-dive Video
References/Resources: / the_crux
How does translation (or protein synthesis) start in prokaryotes? How do 30S and 50S subunits assemble and initiation translation in prokaryotes (bacteria)? What are the different initiation factors involved in prokaryotic translation process? What are the different start codons, and what is so special about the initiator tRNA? What are E P and A sites, and how do they form?
Jump to your favorite section:
00:00 Outline
00:45 Two important points
03:15 E, P, and A site
05:51 30S subunit complex
14:34 50S subunit recruitment
17:33 Bonus information
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Пікірлер: 21
Correction: At 13:36, the AGGAGG (Shine-Dalgarno sequence) is on the mRNA, whereas the UCCUCC is on the 16s rRNA. Somehow, I managed to put them the other way around 😢
Thank you so much for this amazing explanation. It's very clear and to the point, with all the necessary details. It's hard for me to understand the process without visualizing what's happening, and your drawings help a lot. You're such a great teacher of complicated molecular processes!
This is extremely detailed and clear, thank you for creating this and the other videos on your channels. As a chemist, I've noticed two details: - you keep naming complementary sequences in the 3'-5' direction, instead of using standard 5'-3' direction; for example the anticodone of AUG is CAU in the standard order, because the coupling is anti-parallel, but you say UAC. I understand that this is for educational clarity, but at times is not immediately obvious which of the two complementary sequences is named in the standard 5'-3' direction. - at 16:00, the way you note the hydrolysis of GTP attached to IF2 contains a contradiction. You can't hydrolise GTP into GDP and PPi (=inorganic pyrophosphate), because this conjures a phosphorus atom out of nowhere: it's either GMP and PPi or GDP and Pi (inorganic orthophosphate). Which is the case? I hope this is helpful.
Your videos help me in my admision test for PhD, thank you so much 💜
@theCrux
8 ай бұрын
So glad to hear this! Good luck :)
Keep the good work Sir. Amazing job 👏
Thank you - amazing work!
Your videos help me alot for preparation of my UG exam & inspires me to learn more about these topics.THANKS ALOT
Loved it.. please upload full translation.. waiting for more such videos..
Great Video...
Thnk u sir for uploading the video soon...waiting for elongation and termination.....
@Ok25ok26
Жыл бұрын
Uploaded or not?
Good One
Legend❤
Please upload a eukaryotic replication video
amazing job! but how the initiator tRNA positiend itself between the factors before the mRNA come into place?
@theCrux
Жыл бұрын
Please see 10:21 There are many specific details that I could have gone into about tRNA positioning... but that seemed like too much information to include in this video.
Sir from which book you refer to
Sir kya aap profesor h or aap brussels?
❤❤❤😢
Wer lernt noch für Brüser?