Prokaryotic Translation ELONGATION - How do EF-Tu and EF-G move the ribosome? - Deep-dive Video
References/Resources: / the_crux
What is the process of translation elongation in prokaryotes? How does a 70S ribosome translocate? What are the elongation factors involved in the elongation process and how do they work? What are EF-Tu and EF-G and why are they important? How does the Peptidyl transferase reaction occur? How does 23S rRNA catalyze this reaction? How does the ribosome translocate? Find out answers to all these questions in this deep-dive video:
Jump to your favorite section:
00:00 Outline
00:27 70S initiation complex
02:11 EF-Tu
05:29 23S rRNA
06:33 Peptide bond formation
11:03 EF-G and translocation
14:37 Energy consumption
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Пікірлер: 15
Best video on KZread on the topic TRANSLATION.... Very Well explained Sir...👍👍
Great video sir..helping a lot in clearing out all the concepts.. waiting for the next part of the video 👍👍👍👌
Thank you sir, get to understand easily through your video than my lecture notes and videos
thank u for saving my final exam! great explanation..
@theCrux
2 жыл бұрын
I am glad that the content is helpful :)
It is beneficial.
Super sir👌 Can u plz explain what is cis acting and trans acting element
Thank you so much for this crystal-clear explanation! Your handwriting and presentation are super neat. My family who saw me watching this video were also awestruck with the neatness! I have a question: To the A site, an incorrect tRNA can also come, right. So won't it take a lot of time till the right tRNA docks into the ribosome since there are so many different tRNAs and the A site is very small, so only one by one they can come and see if they can fit?
@theCrux
6 ай бұрын
Glad these videos are helpful. And yes, you are correct. Incorrect tRNAs are sampled at the same rate as the correct tRNA - also the reason why ribosomes move so slowly.
@sandhyavasudevan3165
6 ай бұрын
@@theCrux Okay, got it. Thank you!
Hello! Could you explain what is the function of the Ef-Ts factor?
@akhtarulanowar
Жыл бұрын
Recycling of Ef-Tu.GDP to Ef-Tu.GTP
There's 1ATP in forme of 2 energetic bounds used to activate 1 AA there 1Gtp to fix the ribosome and 1 gtp for fixing the rnat-aa on the codon and 1 gtp for moving the 30s and one for termination so badicaly it's 2 atp for activating and dissociating the ribosome and (n-1)×4 juste for moving
@theCrux
Жыл бұрын
I am not sure if I agree with your calculations. Just for elongation: 1 ATP for charging the AA 1 GTP for carrying the AA to ribosome 1 GTP for moving the ribosome and forcing entropic release of tRNA from E-site The release of tRNA from E site and peptide bond formation is entropic, so no energy is used in those steps. In elongation, you DO NOT dissociate the ribosomes. The energy expenditure calculations in elongation should not include Termination step.
U make great videos gg