We need a man like this in every molecular biology class, questioning LITERALLY everything. Because EVERYTHING needs a little further explanation Ms. Sawa!! Lol

I LOVE THIS WAY OF EXPLAINING ......GETTING SOMEONE WHO KNOWS AND ONE WHO DOESN'T KNOW THAT WELL

Hi Sal! I am studying pursuing my undergraduate studying molecular biology with a presentation on Real time PCR tomorrow. I really think this is an amazing process especially with respect to the forensic applications! Thanks for this video, it kind of summed up everything I have already covered so I feel really ready! Khan academy has been great since 8th grade and I am still using it now studying for my MCAT so thank you from the bottom of my heart!

Always love how you guys break down the extent of knowledge to be understood :D

I am a layman but I can still understand PCR completely through this awesome video. Thank you Khan Academy!!!

Best explanation I have seen so far! Yeah Khan Academy!

I had such a hard time understanding this material until now... thank you!

Thank you so much for all the contribution. Much improvement. 😊

Thank you, this helped a lot!

Omg this is guy is awesome how clear he is in his explanation thank you thank you thank you comme to teach to our community college in Glendale Cali please!!!!

Exactly.

@machariawanjagi5353

4 жыл бұрын

precisely

fascinating..I am studying Botany at the university of the free state, this video was really helpfull

soo helpful, Thank you !!

love this.. have a test this friday and it was hard to understand my textbook

I love this stuff so much WHAT

Literally all the questions I had you answered wow thanks.

Great video - thanks Sal!!

Every video that I see with this man’s voice…. I automatically know that I will learn something ❤. Love it

Excellent, thank you

That was really good... Thank you

Helped!

I don’t know if the video mentions it but you can also use pcr to generate multiple copies of a mutated strand by introducing a primer containing a mutant base then favoring only mutant by introducing endonuclease to remove parent strand (mostly like nucleases selecting methylated strands) then repeatly coloniezes bacteria until you got a mutant

i hav understood thanks man

wow amazing!!! thank you

I had a great time listening and watching 👍

Glad and grateful to witness your passion in sharing your knowledge. Bless up!!

THIS VIDEO SAVED ME

Great video, thanks

Wow thank you for the lightbulb moment

One of the best 👍💯

awesome

Thanks so much sir ❤❤

Thank you

i'm a newbie for this entire topic. its clear from this description that only a 'small fragment' (if this is the proper term) of DNA is able to be 'amplified' using this method. but for my understanding, what if the goal is to amplify the ENTIRE DNA sequence (say as in 3 billion base pairs of a human genome). can PCR still be successful to do this - if not, why (and what alternative method would be used)? and if yes, is there anything ''different' about the process for an entire sequence? Thanks in advance, for any insights...

Exactly PCR

*Important video*

Thank you I have a question What is the purpose of the positive and negative control in the PCR reaction?

@craftsandscience860

7 жыл бұрын

shakila rasa the negative control to detect if there is any DNA contaminant in my PCR reaction mixture. After the PCR reaction, the negative control should not produce any band on the gel electrophoresis. The positive control is the control that contain a piece of DNA that will be replicated using my primers and produce a bands, it is just to check that there is nothing wrong with my PCR reaction steps, denaturation, annealing and extension.

To be clear, you do NOT exclusively have to use Taq polymerase. My lab uses Phusion. What matters is that it is thermostable.

We use Pcr at out Food Micro Lab..to make sure food is safe for public

How are the nucleotides added and in what form?

The polymerase is from Yellowstone and the guy who discovered it took it without permission. When the patent was sold for 300 million, he gave no royalties to the park service

@grim8391

4 жыл бұрын

That's tuff

yasss bio

Thermus aquaticus*

@crazybull7282

4 жыл бұрын

Both thermophilus and thermus are used.

@atillaogan3654

4 жыл бұрын

@@crazybull7282 thermus thermophilus is a seperate species

how do you select the right primer when you want to detect a new virus (in case of the first ever testing for that supposedly new virus) ? How do we know about the DNA- when we are trying to figure out if this is a new unknown virus?

@biologywithannie

2 жыл бұрын

Primers can be designed only if the sequence is known. So for detecting a new virus, the first step would be to sequence a sample of it isolated from a patient (using techniques like sanger sequencing) and confirm that it is a new strain of virus. Then you can design the primers by using a sequence that is unique to that strain. Now, you can test if this virus is present in the population by PCR. Only if that unique sequence is present in the patient's sample, will the PCR work and amplify DNA. Hope this helps.

How do you know what exact region you want to make copies of from the original template?

Can u plz subtitle ur videos, as you have done on google.

Can a primer which has been involved in one cycle be utilised again in another cycle? Or is it necessary that a fresh primer is necessary?

@tartanhandbag

6 жыл бұрын

the primer becomes part of the new complementary sequence, so it's not "free", as it were, to go float off and bind to some other DNA strand. in that sense, it has no choice; once it binds it is fixed there. as the cycles go on, eventually the amount of primer (as well as dNTPs) get depleted.

Why do we need to cool it down for the nucleotides to bind with the DNA?

@angeloquimoyog6448

6 жыл бұрын

I think Taq polymerase and primer function at a cooler temperature.

@maratmasry

6 жыл бұрын

Wouldn't it have something to do with proteins denaturing under high temperatures? You would have to cool them down first

can't we clone the dna fragment in a suitable host using vector??...

@mmaking8664

7 жыл бұрын

You can. Transfect bacteria with DNA and the bacteria itself will replicate the DNA

I have a question, why don't we just put the separate enzyme in instead of heating it up?

Why are primers made for both sense AND antisense DNA targets.

@craftsandscience860

7 жыл бұрын

Greg Fox to replicate both of the DNA strand, sense and antisense. If you design primers for only the DNA sense strand for example, it will replicate only the DNA sense strand making the number of copies of this strand higher than the DNA antisense strand

@mostirreverent

7 жыл бұрын

Thanks, I came to realize that a few days after :)

@o2xb

7 жыл бұрын

its to make 2 copies and not just one , therefore making it super efficient!!

♡

I Don't Just love these videos as they help with Mathematics but the person who does the videos sounds alot like the quite popular PokeMon KZread +TheMunchingOrange .

Omg First

Can I find primers on black market?

hey y'all. just curious but what's the difference between this channel and khanacademymedicine?

@taniamukherjee8590

5 жыл бұрын

This for those studying for the MCAT to get into med school, and the other is for those in med school studying for their classes. Just technicality but yeah some of the mcat videos dont cover things beyond the scope of the mcat

She sounds like Alexandria Ocasio-Cortez

new-KLEE-yo-tides or new-CLAY-yo-tides?

This isn't the best explanation of PCR, but it helps summarize.

how does RT PCR work?

@SuperHulk1989

6 жыл бұрын

It's the same just add prime and reverse transcriptase to your RNA you will get one DNA strand and then use the same steps in the video...

Thermos Aquaticus found in hot tubes in Yellowstone

How come you have an MCAT tutor that hasn't made PCR before?

@josesomohano6118

7 жыл бұрын

Same way I can teach you how to do CPR without actually ever having to perform it on live person.

@manuelberenguerrojas7271

2 жыл бұрын

I did PCR in my second year of college. In four years I’ve done it over 5 times.

Why all the great teachers on KZread .Why do we need to pay for college by providing us with such an average teaching?

He pronounces nucleotides wrong. :/

Who is the professor here guy or the girl😂

:)

My dad says that this is actually the correct way to pronounce "nucleotides". And I think he knows what he is talking about because he has a BA in Genetics

i ship yall

Info is good, but terribly drawn... The difference between the shortened and original strand is almost non-existing. Make it shorter, use another color or something along those lines.

I'm cringing at how he pronounces "nucleotides."

@ss-nu8xx

8 ай бұрын

Yeah keep focusing on the unintelligent aspects. Low IQ

Seek Christ Jesus YHVH

FORTNITE OR FAIL TEST

Her voice and use of words is really annoying (as she would talk to kids), exAAAActly...