This instructional video walks you through the workflow for using the Olink Target 96 platform with the Olink Signature Q100 instrument.
Chapter 1: Introduction to Target 96. An introduction to the Proximity Extension Assay (PEA), the Olink Target 96 kit and the Olink Signature Q100 instrument.
Chapter 2: Before you start. Lab work tips and tricks including how to perform reverse pipetting (2:34) and proper plate vortexing (3:31, but also, see • Olink Vortexing instru... for further plate vortexing tricks).
Chapter 3: Incubation step. The first day of lab work is all preparation before the incubation; setting up the control strip, making the incubation mix and setting up the incubation plate with your samples and the incubation mix.
Chapter 4: Extension and amplification step. The second day starts with preparing your PCR instrument, preparing extension mix, adding the extension mix to each well of the incubation plate and running the PCR.
Chapter 5: Detection step. With our extension product ready, the final step of the protocol starts with priming of the 96x96 IFC (10:45), preparing the detection mix and mixing the extension product and detection mix in the Sample plate. Finally, the primers and samples are added to their respective sides of the primed IFC and the IFC is run on the Olink Signature Q100 instrument.
0:00 Introduction to Target 96
1:50 Before you start
4:09 Incubation step
7:28 Extension and amplification step
10:45 Detection step