Mushroom Extraction - Bath vs Probe Sonicator - Side-by-Side Comparison

Please visit www.hielscher.com/mushroom for more information about Hielscher sonicators and ultrasonic extraction!
In this video, we compare the extraction power of an ultrasonic bath - also known as an ultrasonic cleaner - with that of a Hielscher UP100H ultrasonic probe. The ultrasonic bath has a maximum output power of 70 watts at 40 kilohertz. The Hielscher UP100H sonicator with 14-mm probe has a maximum output power of 100 watts at 30 kilohertz.
For this demonstration, we use two identical beakers. Each beaker contains 20 grams of crushed dried Chaga mushrooms. We add 180 ml of a cold water-ethanol mixture. A higher alcohol concentration improves the solubilization of water-insoluble components such as betulin, betulinic acid, or phytosterols.
Non-thermal extraction at minus 10 degrees Celsius preserves the heat-sensitive components. This results in a better extract quality and a higher concentration of bioactive constituents. This can lead to a higher potency.
The first beaker is sonicated for 5 minutes in an ultrasonic bath. We sonicate the second beaker with the UP100H sonicator for 4 minutes and 30 seconds - 30 seconds less than in the ultrasonic bath.
After sonication of both beakers is complete, we separate the solids from the liquid using a French press filter. The extract from the UP100H probe-type sonicator is much darker compared to the extract from the ultrasonic bath.
The cell walls of mushrooms contain chitin. The chitin makes the cell structure of mushrooms very rigid and difficult to break or penetrate. Therefore, very strong cavitation is needed to extract mushrooms.
To quantify the difference, we use ultraviolet-visible spectroscopy. Ultraviolet-visible spectroscopy is used to characterize botanical extracts and to compare the extract quality and concentration of the two sonication devices. The UP100H sonicator extraction curve is significantly higher than the curve of the ultrasonic bath over the entire absorption spectrum from 300 to 800 nanometers. In addition, there is a significant increase in yield in the 340 to 440 nm range.
The UP100H probe sonication produces a much better extract profile due to the more efficient extraction. The intense cavitation of the UP100H sonicator extracts water-soluble and water-insoluble components. The ultrasonic bath with its low intensity cannot achieve the same extract quality or yield.
Scientific extraction studies confirm the superior efficacy of Hielscher's ultrasonic technology over conventional methods like bath sonicators. "Probe sonicators fared better than bath sonicators in extracting bioactive compounds while ultrasound assisted with enzymes was more efficient than conventional or alkali extraction methods." (Inguanez, L.; Zhu, X.; de Oliveira Mallia, J.; Tiwari, B.K.; Valdramidis, V.P. Extractions of Protein-Rich Alaria esculenta and Lemna minor by the Use of High-Power (Assisted) Ultrasound. Sustainability2023,15,8024. doi.org/10.3390/su15108024 )
After separation, you can use a rotary evaporator - a Rotovap - to remove alcohol and water at mild temperatures to produce a concentrate.
With the UP100H sonicator you can also extract other botanicals, such as:
- cannabis,
- medicinal plants,
- coffee or tea,
- herbs or spices.
For larger batches or inline processing, Hielscher manufactures ultrasonic homogenizers with more power, such as the UP400St (400 Watts) or industrial inline reactors.
We look forward to discussing your extraction project with you! Please contact us or visit us today at hielscher.com/mushroom!
Hielscher UP100H 100 Watts Homogenizer: www.hielscher.com/100
Hielscher UP400St 400 Watts Homogenizer: www.hielscher.com/400
Hielscher industrial ultrasonicators: www.hielscher.com/industry.htm

Пікірлер: 53

  • @user-nr7xl4gd5d
    @user-nr7xl4gd5d Жыл бұрын

  • @kellykim4794
    @kellykim47945 ай бұрын

    I wonder how long you can use the extracted liquid in room temperature without going bad. And extracting solvent, should I use water , alcohol, or oil? What percentage of the plant can you extract? Please answer me for this. Thanks

  • @HielscherUltrasonics

    @HielscherUltrasonics

    5 ай бұрын

    @kellykim4794 This is not easy to answer. You will have to test various protocols and formulations. We will be glad to help you with your extraction project. Please contact us directly!

  • @LoversBe1
    @LoversBe1Ай бұрын

    curious how chaga is grinded? can you please share with me how this is acheived? what sort of machine is best? i have a bunch of chaga and also looking into a sonicator. i really want to make the highest quality extracts.

  • @HielscherUltrasonics

    @HielscherUltrasonics

    Ай бұрын

    @LoversBe1 You can buy ground Chaga powder. Else, some kitchen blenders work for chunks smaller than 10 millimeters to get to a powder. For highest quality extracts and good yields, get a sonicator! We will be glad to help you with your project. Please contact us directly. Thank you for your attention. Subscribe for more updates and professional content: 👉 kzread.info/dron/G1xa_8clP9cRlq7kIcWQug.html

  • @vhyombhatt3935
    @vhyombhatt39353 ай бұрын

    For mushrooms, is it true that a certain frequency of waves should not be exceeded in the ultrasonic spectrum? if exceeded more than the value, the molecules to be extracted will also be damaged? kindly guide on this

  • @HielscherUltrasonics

    @HielscherUltrasonics

    3 ай бұрын

    @whyombhatt3935 Not true! Ultrasonication produces cavitation. Cavitation extracts from the plant. The ultrasonic wavelength is much to long to affect molecules directly. It’s always the cavitation that produces the effects. 👉 kzread.info/dron/G1xa_8clP9cRlq7kIcWQug.html

  • @Kevin-ox7zp
    @Kevin-ox7zp7 ай бұрын

    What solution should i use for extracting cyanocobalamin? And what type of ultrasonicator should i use if i want to extract it from mold?

  • @HielscherUltrasonics

    @HielscherUltrasonics

    7 ай бұрын

    @Kevin-ox7zp: Cyanocobalamin is water-soluble. Although it can also dissolve in dimethyl sulfoxide (DMSO), which is a non-toxic, polar aprotic solvent that mixes well with water, the need for such an advanced solvent may not be necessary in all cases. I recommend starting with a mixture of water and the substance from which you wish to extract cyanocobalamin. The choice of the most suitable ultrasonicator depends on the volume you need to process. For smaller samples ranging from 50 to 100 mL, consider the Hielscher UP100H, a 100-watt sonicator available at this link (www.hielscher.com/UP100H). Please feel free to contact us directly, and we will be happy to assist you with your extraction process.

  • @eiem9879
    @eiem98796 ай бұрын

    What is the recommended time for making extracts?

  • @HielscherUltrasonics

    @HielscherUltrasonics

    6 ай бұрын

    @eiem9879 This depends on many factors. Please contact us directly with volumes, solvents, mushroom types etc. We will be happy to provide recommendations.

  • @anthonyackerman5556
    @anthonyackerman55563 ай бұрын

    Wouldnt it work if you have the same area of mass as well the same watts?

  • @HielscherUltrasonics

    @HielscherUltrasonics

    3 ай бұрын

    @anthonyackerman5556 The difference between probe type sonication and an ultrasonic cleaning bath is the intensity - the power coupled per surface area. Due the technical design of an ultrasonic bath, the amplitude - this is the mechanical displacement of the surface in contact with the liquid - is limited to approx. 1 micron. Hence, a cleaning tank couples the power across a large surface area at very low intensity. Ultrasonic probes have adjustable amplitudes of up to 100 micron or more. This couples the power using a very small surface at a much higher intensity. The intensity of an ultrasonic bath is much too low to effectively break chitin and cell wall structures. Thanks for watching! Please subscribe to our channel for more interesting videos!

  • @sjoerdhb7507
    @sjoerdhb75078 ай бұрын

    What is the ratio of ethanol to water you use?

  • @HielscherUltrasonics

    @HielscherUltrasonics

    8 ай бұрын

    @sjoerdhb7507 Approximately 6:4 Ethanol:Water. What would you like to extract? Please contact us directly. We will be glad to discuss your extraction project and recommend the most suitable setup. Thanks for watching!

  • @timm5358
    @timm5358 Жыл бұрын

    The bath costs €3000.. not a solution for at home.. unless you have €3000 laying around somewhere

  • @HielscherUltrasonics

    @HielscherUltrasonics

    Жыл бұрын

    @timm5358 If the bath simply cannot extract properly, you waste €100 and got yourself an expensive jewelry cleaner 😆

  • @jhonsmith5567

    @jhonsmith5567

    10 ай бұрын

    right, this couldn't be directed at normal consumers, regular people would buy the 100$ one and just run it for longer vs spending 3000$

  • @HielscherUltrasonics

    @HielscherUltrasonics

    10 ай бұрын

    @jhonsmith5567 That’s like trying to drive a nail into the wood by tapping it with your finger for half an hour. Running something at much lower intensity for longer is not the same as high intensity for a short time. Hope that helps 😉

  • @Jake-jj1zs

    @Jake-jj1zs

    9 ай бұрын

    ​@@HielscherUltrasonics Looking into your products but don't have the money at the moment, plan on buying in the future. But I am wondering even if you were to run the water bath method for 24hours with the extract directly in the stainless pan, it still would not produce similar quality extract? Glad I came here, saw this video and comments, as I had been browsing water baths.

  • @HielscherUltrasonics

    @HielscherUltrasonics

    9 ай бұрын

    @Jake-jj1zs If you sonicate in a bath for 24 hours, the liquid will get hot (boiling). This results in a thermal degradation of the bioactive compounds. Even with pauses for cooling, the low intensity of bath in insufficient for breaking the cellular structure or overcoming the limited permeability of the mushroom matrix. Therefore, a cleaning bath cannot extract the same quality or quantity of bioactive compounds as a probe sonicator.

  • @Steve_toasty
    @Steve_toasty8 ай бұрын

    Couldn’t you use citric acid instead of alcohol?

  • @HielscherUltrasonics

    @HielscherUltrasonics

    8 ай бұрын

    @Steve_toasty Very good question. Yes you can use citric acid to assist the extraction of a specific extract profile.

  • @Steve_toasty

    @Steve_toasty

    8 ай бұрын

    Thank you for the reply ✌🏼

  • @glenmarshall8168
    @glenmarshall8168Ай бұрын

    The price tag, though 😮

  • @HielscherUltrasonics

    @HielscherUltrasonics

    Ай бұрын

    Hielscher sonicators are built for years and years of production use and to deliver excellent performance. They are great value for the price. Buy cheap? Buy twice (or more).

  • @seanyjon3667
    @seanyjon36679 ай бұрын

    So im looking at an Ultrasonicator that only goes up to 20khz. that should still be enough power to achieve these results no?

  • @HielscherUltrasonics

    @HielscherUltrasonics

    9 ай бұрын

    @seanyjon3667 The frequency of 20kHz does not say anything about the power or amplitude. Please contact us directly! Our technical team will be glad to explain this.

  • @DanielSchweinert
    @DanielSchweinert5 ай бұрын

    Of course the cheaper bath is not that efficient because of it's design and higher volume but if you take out the piezo element make the wires watertight and drop it into your glass container you get almost the same effect for much cheaper. It's ridiculous how much these probe sonicators cost (3000,- EUR and upwards).

  • @HielscherUltrasonics

    @HielscherUltrasonics

    5 ай бұрын

    @DanielSchweinert: 🤣🤣🤣 No you don’t. You don’t get even close in amplitude or intensity.

  • @DanielSchweinert

    @DanielSchweinert

    5 ай бұрын

    @@HielscherUltrasonics there are some DIY sonicator tutorials on YT to mimic the UP50/100 but it involves to make a horn on a lathe and tune it to the right resonance frequency.

  • @HielscherUltrasonics

    @HielscherUltrasonics

    5 ай бұрын

    @DanielSchweinert : I suppose, I know the video you are talking about. Performance sonication is about getting power into the liquid - effectively, efficiently and at specific sonication parameters (e.g. amplitude). Ultrasonic effects change drastically with sonication amplitude (and pressure when using a flow cell). 125 micrometers of vibration cause a much different cavitation intensity than 1 micrometer or 20.

  • @DanielSchweinert

    @DanielSchweinert

    5 ай бұрын

    @@HielscherUltrasonics Im new to this ultrasonic stuff but Im learning fast. I just want to do some lions mane tincture but cant spend thousands of EUR for a simple sonicator that consists only of a driver board, transducer and a tuned horn. There must be a cheaper way to break up that chitin cells with other science.

  • @DanielSchweinert

    @DanielSchweinert

    5 ай бұрын

    @@HielscherUltrasonics vielleicht benötigt ihr ja professionelle Produktfotos von so einem UP100H Gerät? Ich hab ein Studio :-)

  • @jaydenimbriano5690
    @jaydenimbriano56902 ай бұрын

    could you not just leave it in the ultrasonic cleaner for twice as long instead and probably get the same result

  • @HielscherUltrasonics

    @HielscherUltrasonics

    2 ай бұрын

    @jaydenimbriano5690 Will you cut through steel with your flashlight, if you shine on it for twice as long as with the cutting laser? Time is no substitute for intensity. We appreciate your interest in our videos. To stay updated with our latest content, please consider subscribing: 👉 kzread.info/dron/G1xa_8clP9cRlq7kIcWQug.html

  • @jaydenimbriano5690

    @jaydenimbriano5690

    2 ай бұрын

    @@HielscherUltrasonics I don't think that's really a fair comparison in this case. If you extract mushrooms in alcohol you will extract much more leaving them to soak in 6 weeks then one week with no added intensity

  • @HielscherUltrasonics

    @HielscherUltrasonics

    2 ай бұрын

    @jaydenimbriano5690 In the absence of sufficient mechanical stress capable of disrupting cellular structures and emulsifying insoluble elements, the extraction process is limited to the fraction that is soluble in alcohol and capable of diffusing through cell membranes.

  • @HielscherUltrasonics

    @HielscherUltrasonics

    2 ай бұрын

    Comparing the cleaning bath to the UP100H probe sonicator is like pitting a kitchen knife against a heavy-duty chainsaw for tree cutting - it's just not a fair match. The cleaning bath simply isn't designed for botanical extraction tasks, especially not for dealing with the robust Chitin-based cell structures found in mushrooms. The cleaning bath is well suitable for cleaning jewelry or even carburetor nozzles, but when it comes to extracting substances from materials like mushrooms, it falls short due to its very limited intensity. Even giving it an extra 30 seconds of sonication time doesn't bridge the gap in effectiveness compared to the UP100H probe sonicator. So, while the cleaning bath has its uses, extracting mushrooms isn't one of them. Thank you for engaging with our video. Enhance your experience by subscribing for more professional insights: 👉 kzread.info/dron/G1xa_8clP9cRlq7kIcWQug.html

  • @EgonSorensen
    @EgonSorensen5 ай бұрын

    "Non-thermal extraction at minus 10 degrees Celsius" 0:47 Then the solution is lowered into a 19 degrees Celsius hot bath - I guess that the solution didn't stay at -10 [C] for verrrry long ;ø)

  • @HielscherUltrasonics

    @HielscherUltrasonics

    5 ай бұрын

    @EgonSorensen: Thermal (= non-non-thermal 😉) extraction mostly refers to exposure to 70 degrees Celsius or more for long periods. In thermal extraction you are just cooking your materials for extraction. At lower temperatures or short exposure, you don’t get much extraction - unless you use sonication. The long exposure to high temperatures of thermal extraction causes heat degradation of materials and bioactive compounds. We will be glad to assist you with your extraction project. Please contact us directly! Don’t forget to subscribe to our channel for more interesting videos.

  • @EgonSorensen

    @EgonSorensen

    5 ай бұрын

    @@HielscherUltrasonics You say that a lower temperature results in better yields and extract quality - then you show a UV VIS Overlaid Spectra showing the difference in quality/yield. If the chilled solution, vs. room temperature solution, quality/yield wise is the method of sonication, then why bring up temperature? I was searching for videos on Lion's mane and how to grow them, benefits, etc - and your video came up. Interesting, and just to be clear - it is not the temperature (mostly - say >80%) but the applied method of sonication that determines yield/quality. Thanks!

  • @HielscherUltrasonics

    @HielscherUltrasonics

    5 ай бұрын

    @EgonSorensen: This video is to compare two non-thermal ultrasonic extraction setups. Many people think sonication is all the same - no matter what setup, intensity or power. That is not true. An ultrasound bath produces a mere 1 micrometer amplitude. While sufficient for cleaning jewelry, this is insufficient for breaking cell walls. The UV Vis overlay is to show that difference resulting from different sonication settings. A thermal/non-thermal comparison would be a topic for another video. However, it’s commonly known, that various lionsmane compounds are heat sensitive. Thanks for watching!

  • @LoversBe1

    @LoversBe1

    Ай бұрын

    what if the plant material is saturated in water and then frozen like in the case of chaga. wouldnt the freezing break the chitin cell walls and then be more effective in a sonic bath? yet perhaps this is still not as good as a wand. would love to hear your input. thanks!! 🙏

  • @HielscherUltrasonics

    @HielscherUltrasonics

    Ай бұрын

    A single freeze-thaw cycle won’t be enough. Also just breaking the cell barrier won’t extract much. Sonication emulsifies insoluble compounds and assists transport from the cells to the liquid phase.