Microscopy: Measuring The Point Spread Function (Nico Stuurman)
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Пікірлер: 10
@aatt32093 жыл бұрын
It's incredible to see the astigmatism & spherical aberration demonstrated with the beads through the z-stacking. Nice job!!!
@oljazaitseva46889 жыл бұрын
Thank you for such a clear explanation.
@DaruoshAghajaney4 жыл бұрын
What a machine!!!
@kaleemullah81428 жыл бұрын
Nice Lecture.
@scientia_potentia_est6 жыл бұрын
thanks for the vid! One question though, do you know the reason for those asymmetrical 3D PSF that you got on the Nikon system. Was it that the obj didn't have appropriate spherical aberration correction for the particular emission excitation and/or emission wavelength that you were imaging at? Or was it because of wrong immersion media and/or lack of coverglass thickness correction? Just trying to learn about different sources that cause these asymmetrical 3D PSF.
@zz6761 Жыл бұрын
How to do with tetraspeck? Does it also use ethanol to dilute?
@artityagi55386 жыл бұрын
in my system, sometimes the rings appear in one direction, sometimes in the other and sometimes in both the directions- totally inconsistent. why would that happen?
@jaytaffer96418 жыл бұрын
What are those purple syringe tools? Do they measure a specific amount of a substance? What are they called?
@ike4594
7 жыл бұрын
micropipette... for measuring liquid volumes from 1ml to under
Пікірлер: 10
It's incredible to see the astigmatism & spherical aberration demonstrated with the beads through the z-stacking. Nice job!!!
Thank you for such a clear explanation.
What a machine!!!
Nice Lecture.
thanks for the vid! One question though, do you know the reason for those asymmetrical 3D PSF that you got on the Nikon system. Was it that the obj didn't have appropriate spherical aberration correction for the particular emission excitation and/or emission wavelength that you were imaging at? Or was it because of wrong immersion media and/or lack of coverglass thickness correction? Just trying to learn about different sources that cause these asymmetrical 3D PSF.
How to do with tetraspeck? Does it also use ethanol to dilute?
in my system, sometimes the rings appear in one direction, sometimes in the other and sometimes in both the directions- totally inconsistent. why would that happen?
What are those purple syringe tools? Do they measure a specific amount of a substance? What are they called?
@ike4594
7 жыл бұрын
micropipette... for measuring liquid volumes from 1ml to under
what is the name of the software?