Nice lecture. For the hard question. I assume at low pH the ester at the bottom will become protonated. If you move a pair of electrons from one of the sugar oxygens attached to the tricyclic rings at the top of the molecule. You can move them around (curly arrows) to release the carboxy group at the bottom to give you the extended conjugated structure which will now fluoresce. The freed up carboxyl group can then be used for further conjugation reactions. Very neat.

Amazing video, totally need this for flow cytometry!

Thanks a lot sir... Wonderful video

Wow! Real life Sheldon!

Which probes can be used for targeting of lysosomes

What software can be used to create a video like this?

@lbochtler

6 жыл бұрын

pecatum666 adobe after effects with a screencapture software and a greenscreen as a background.

This is one of the lectures I found it very informative. Especially the HOMO and LUMO relation. Most lectures they just skip this fundamental principle. By the way, why not call HOMO highest occupied ELECTRON orbital instead of molecular? after all, it is the electron causing such phenomena. Thanks!

@mrmadmaxalot

8 жыл бұрын

+Wulin Teo This is an old comment, but since no one responded, the reason is because the term comes from molecular orbital theory which makes distinction between atomic orbitals and molecular orbitals. Since these are molecules the behaviour of the molecular orbital is what they are working with.

@WulinTeo

8 жыл бұрын

+bv Thanks!

Are Fluorescence labels and Fluorescence probes are same ??

GREAT!!!