Extremely thorough and well-prepared discussion of a topic in which I had no previous understanding. Totally great!

That's just extra ordinary talent. To make such a complicated thing so simple

If only this video was in higher resolution...

@scicommlab

8 жыл бұрын

+Andrew Areva We re-recorded this video a couple of years ago with higher resolution. Check it (and a lot of other microscopy videos) here: kzread.info/dash/bejne/Y4VpuJZtYrmdqKw.html

@brandond8488

2 жыл бұрын

@@scicommlab Great use of super-resolution imaging 👀

@ErichAmMeer

2 жыл бұрын

try confocal

Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks

Thank you so much for this excellent presentation!!! ❤

Excellent video. Very easy to follow. Thank you!

Thanks for an excellent, easy to follow introduction to confocal microscopy! That would have taken me hours to read:) Keep up the great work.

Excellent presentation, very didactic, thanks Kurt

Thanks for this wonderful video

Thank you. Very precise and informative.

Good explanation of the diagrams. Thank you!

Very helpful, thanks!

Excellent presentation!

Thank you for the amazing explanation

Fantastic. Very well explained.

awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though. Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512x512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe

Thank you so much!!

Thank you for making this video.

Thank you is very much for the video!

Very nice video! thanks for uploading :)

Thanks, Thanks ! So great video.

Great help. Thanks!

Thanks for great explanation

Excellent work Kurt!!! I've addad a link on my blog -

Great video.

Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?

explained very clear.

excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you

very clear... helped me a lot... thanks..:)

Thank You very much

Excellent!

this is such a good one! Thank you so much

Thank you!

fantastic!!

I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?

Confocal and multi-photon way to go! Spent years in research using, and later selling LOL!

Nice one!

a life saver !

is there a difference between the dichromatic mirror and dichrioic mirror?

Great! Thanks

very interesting thanks

Excelente! Muchas gracias, esto me ayudó mucho. :-)

Awesome!

awesome!

yes... Awesome!

Very nice presentation there. However, if it was in HD (720p or better), I would have seen and maybe understood more ;)

This sounds inspired by early innovations in the history of television.

wait.. working of spiral spinning disc is not very clear..

13:45 Apparatus. 18:28 Spinning disk. 22:30 Multi-photon.

Thanks a lot

Confocal Microscopy starts at 3:50

hell yes

need elctron microscope for hiv diagnoses

spinning spiral mirror working is not very clear

LIKE.

Opino. Que. Diyegnnoñojia. Es. Maravillosa. Pero. Soy. Una. Simple. S🎃😊😅😂😂

like

unsharp unsharp unsharp unsharp unsharp ..................................

If only microscopists and histology people added scale bars. Don't show me an image without context!

"you can see that there is stuff that is clear and sharp and in focus.." yeah.. nah not really :'D

Why is this potato quality?

@darlantro

8 жыл бұрын

uploaded 5+ years ago. KZread had harsher compression requirements back then.

@darlantro

8 жыл бұрын

also, probably an old camera

Thank you so much for this excellent presentation!!! ❤

Very helpful, thanks!

Thank you so much!!

Awesome!

very helpful, thank you :)

Awesome!