Small tip to consider: for precise cutting circles the best solution seems to be the Olfa Compass Circle Cutter. I made my darkfield filter with it and it turned out perfect. Way better circles than those cut with scissors :)

It is also important to know that light source can affect patch stop size. My microscope has a single small led as a light source so my patch stops have to be much much smaller than these. I still can’t find a way to achieve darkfield with the 40x objective but the 5x and 10x are working great

@Mythreesons137.

2 жыл бұрын

I know what you mean they need to design a variable dark field condenser plus objectives need a variable iris which is no big deal why ?

@nightstormoutdoors6080

2 жыл бұрын

@@Mythreesons137. I need to adjust the condenser's focus for the home-made disk I made to work. The background I get is more grey than black.

Thank you for the video. It makes me wonder why more scopes don't come with these filters, I would guess these are more useful that a blue filter.

@JoeMama-gr3dt

Жыл бұрын

It's only because the filters have to be pretty cheap if you have to sell them with the microscope and it's profitable to sell them separately

I was lucky enough to find a friend with a 3D printer who ran up a set of filters for me. The best bit was that he printed them in blue and this provided some great effects, so just suggesting if you follow Oliver's advice, you try a few patch colours in at least a mid to dark shade.

I love this channel. But is there any videos about DIY polarization microscopy? I made darkfield mask and also i am enjoying a new world with polarization. It adds a lot of new information in the scope. It lets me discover new objects that are hidden otherwise. And i can use the polarization to change from brigthfield to DF in a second. Please do a tutorial for this

thk u mr oliver kim helpfull for my microscope

thank you a lot

Thank you, as always very instructive and educational. Any chance you'll do a video on fluorescence dyes (DAPI,Acridine, etc) ? Not a lot of info out there.

Nice i loke your all videos but can u make a video for that ho to upgrade and make dark field in kids mirror reflecting microscope

Good video. I will try it. I will go out and buy some transparent tejp, print out some black circles, and buy some grapes - Check.

I'm gonna try to make my patch stop by cutting electrical tape into a circle and tape it right to some projector paper. Thanks for your videos. Learning a ton.

@davewinch7677

6 ай бұрын

That is what I ended up doing. For my microscope, I took a triple A battery and stuck some tape to the bottom of it and trimmed around it with a pair of scissors. Stuck that circle on to a piece of heavy plastic, cut to the proper shape and I had my filter. Works great with other colors also.

And i note that u change your background microscopes

Thank you :)

@makSyak

3 ай бұрын

ill try this when i get my micrscope

Thank You! You are an AWESOME scientist!

Buy watch glass cover in 32mm ( or you size).then glue on a dime, pennny, nickel, quarter. Of paint on dots

Hi Oliver! I went into the glass shop near and asked them to cut me a glass filter just like the blue filter that came with the microscope. Then I glued a black plastic patch stop to the middle. It worked well upto a few times, then it started to look very blurry even though I cleaned it. I also tried to make from plastic boxes like you do, but those boxes are not as transparent as glass. Anyway I’m just thinking what could have happened? Also does it really work at x400 aswell because for me at x400 the background and the specimens too are completely pastel white instead of black background and white specimens and I can’t almost distinguish anything.

Awesome! I have a SW380T but can't achieve this results. I tried to manually make darkfield filters but they are far from perfect. Did you change the lighting?

@MicrobehunterMicroscopy

10 ай бұрын

no change in lighting. the disk of the filter must have the right size.

Hello, I am using a Swift SW380T like the one you have but i have a problem with darkfield. it seems like my slides are too dark when i use 40x objective. I tested 15/16/17 mm patches but it's not really working as it should. Am i messing smth else up maybe?

oh so to use darkfield I need an expensive microscope not an inductory one (sw150) but why in my first microscope toy microscope at highest magnification it was dark and at lower it was white

Will any filter holder and condenser work for any microscope

@MicrobehunterMicroscopy

5 ай бұрын

Short answer: no. The parts are not standardized.

Put subtitles on the videos please.

@JM-md6vt

2 жыл бұрын

En español?

@mundomicroscopico-microsco4830

2 жыл бұрын

@@JM-md6vt puede ser

@MicrobehunterMicroscopy

2 жыл бұрын

There are already automatically generated subtitles by youtube in English. You can turn them on by clicking on the icon on the lower right on the video.