Please show how this is done manually without excel

Thankyouu,

with using a Bio Rad plate we obtain a number that is absorbance how we can convert the Vo in concentration/time ?

@azhietheresetangang6576

3 жыл бұрын

In this video I can't really figure out the calculation of the y intercept from the graph.

Does this mean that the approximation method is very wrong? Because the answers for Km and V max are very different

@dr.g5043

2 ай бұрын

Approximation is inherently flawed if your goal is to get a correct answer. However, it can be a very good guide in many instances. The dataset I show here intentionally creates a situation where the approximation is misleading because the dataset is not complete - the measured velocity does not approach Vmax. Consequently it is not possible to accurately estimate Vmax, which means the Km estimate is also flawed. Conversely, the Lineweaver-Burk approach also has some inherent issues that I don't discuss in this video which can result in incorrect Km and Vmax values.

Thank you, it helped so much now haha

@G.p.p.G

10 ай бұрын

Perché b=0.004?

How did you calculate Vo ?

@dr.g5043

Ай бұрын

These would come from individual experiments where you are monitoring the rate of the reaction. For example, if you set up an experiment that monitors the absorbance of a product and plot it vs. time, the slope of that line would be related to the rate - if this is done under controlled conditions, that would be your V0