Lecture 32 Isolation and Purification of Proteins
Protein purification, IPTG, cell pellet, lysis buffer, FPLC system, PMSF, lysozyme, sonication, Ni-NTA column, BME, SDS-PAGE
Protein purification, IPTG, cell pellet, lysis buffer, FPLC system, PMSF, lysozyme, sonication, Ni-NTA column, BME, SDS-PAGE
Пікірлер: 34
Can't believe so much information of soo much value this video offers!
Very comprehensive and methodical presentation. Kudos to the presenter.
Very interesting and well done. An excellent education material for our students! Thanks a lot.
This is a awesome video , very helpful. Great demonstration. Accurate protein purification demonstration
Thankyou so much . Cleared all the doubts .
This is the excellent, could you please make a video on mammalian expression systems eg. CHO-S and HEK293T cell lines.
A great work indeed!!! Too much valuable information in a single video... Carry on this work please... My first ever comment on you tube... You just compelled me for appreciatiom. Many Thanks
Amazing teaching madam, thank you so...much
Helpful video 😊
Really helpful. Thankyou.
Great video...
It helped me a lot mam for my protein estimation.
great video
Thank u so muchhhhhh
Thank you mam
Well explained....nice
Sangat mudah dipahami 🙏
nice method
Thank you mam this is a best valuable video mam please make more videos like this so that it will be helpful for different students on the field of research.
its all useful for gate xl
Nice video! Which protein did you purify?
Can you tell me how much quantity of lysis buffer you have used for resuspension the cell pallet
She is pretty
What will be the effect of ultrasonication and centrifugation on the fat content in the sample. I want to remove only protein from my sample and keep my fat inside the sample..is it OK to use these processes to keep fat in the sample?
very helpful and informative video maam.. i have one question ..what if the ice melts ? is the temperature maintained inside is cold ? or what kind of ice should we use maam ?
How we extract protein from animal tissue pls guide
What is meant by PI ?
@himanshupanda1690
3 жыл бұрын
Isoelectric Point...At which pH the net charge of the protein becomes ZERO
@shardabharti2091
3 жыл бұрын
Isoelectric point
madam can you share the email please I want to discuss about my samples. Thank you
Aduthathada pls kojam tamil la soluga
@tamilbiology5078
2 жыл бұрын
It's a central university bro tamil ah sollamaatanga
@rajavishnuvardhana6830
2 жыл бұрын
She is not talking about Sangam literature here...