How to Use an Autoclave

There are a lot of products that need to be sterilized: pharmaceuticals, equipment, solutions, medical waste, and even tattoo needles! We use a device called an Autoclave to sterilize materials in the lab. In today's demonstration, we'll show 2 common applications:
1. Sterilize a solution using slow exhaust.
2. Sterilize and dry some empty glassware using fast exhaust.
You can explore our interactive autoclave for free at www.ncbionetwork.org/educatio....
*Special thanks to Meghan Davis, Toby Mapes, and Rowan-Cabarrus Community College for making this project possible.

Пікірлер: 56

  • @frederick-nrunkkamara103
    @frederick-nrunkkamara1037 жыл бұрын

    Autoclaves have come along way since I autoclaved media broth

  • @raishahaddad9705
    @raishahaddad97055 жыл бұрын

    I love and supported the people who making this educational videos 😄😄😄

  • @vatsaldabhi1145
    @vatsaldabhi11453 жыл бұрын

    superb to the point explanation!!!

  • @TomsonGaming
    @TomsonGaming9 жыл бұрын

    love educational videos like this helped me alot:)

  • @autoclavedepot1242
    @autoclavedepot12425 жыл бұрын

    Nice job mate!

  • @Usedstryker
    @Usedstryker9 жыл бұрын

    An autoclave is an expensive medical tool and should be handled properly. If you are in doubt on what to do, always consult the user’s guide.

  • @cebidexpharmaceutical6314
    @cebidexpharmaceutical63145 жыл бұрын

    thanks fast and to the point information...

  • @8372cd30
    @8372cd302 жыл бұрын

    ty! This helped a lot

  • @nudratfatima5755
    @nudratfatima575510 жыл бұрын

    very easy for us to explain students

  • @ACG099
    @ACG09910 жыл бұрын

    Thank you very much... it is really a good video to understand the basic mechanism of autoclave... GOD bless you muffin choc answer to your question is "yes", you would use the same procedure but "fast exhaust" one because you are just sterilizing beakers (which are empty glasswares)... remember... liquid turns solid slowly, so slow exhaust for liquid media's and .... solid melts fast, so fast exhaust for solid things (i.e. instruments or empty glass wares) Regards, Dr. Ammar

  • @me-de6hl

    @me-de6hl

    Жыл бұрын

    Hello, thank you

  • @jjptech
    @jjptech11 жыл бұрын

    Thank you for the video. Do you know any FDA or ISO guideline for ussing propperly the autoclave? Thanks.

  • @ashwini16k
    @ashwini16k7 ай бұрын

    Thank u so much 🙏🏻

  • @jillian3047
    @jillian30477 жыл бұрын

    Cramming for a certification exam tomorrow. Thank you!

  • @captin__7658

    @captin__7658

    6 жыл бұрын

    Jillian What exam did you take? I hope you passed 🙂

  • @ilyasjamei1413

    @ilyasjamei1413

    3 жыл бұрын

    @@captin__7658 hello , are you still here?

  • @captin__7658

    @captin__7658

    3 жыл бұрын

    Ilyas Jamei yes?

  • @vantablack1119

    @vantablack1119

    3 жыл бұрын

    @@captin__7658 no

  • @dainanaorbe6965
    @dainanaorbe6965 Жыл бұрын

    May I know the specifications of this autoclave. Thank you. Planning to buy this kind of autoclave. Thank yoj for the response

  • @reyakurd8810
    @reyakurd88104 жыл бұрын

    thank you for video

  • @tandy2740
    @tandy27403 жыл бұрын

    How do you change the length of sterilization time? And about how long does the entire process take (for autoclave to reach the proper temp/pressure, about 15 sterilization, then de-pressurize/cool down)?

  • @funny11744

    @funny11744

    7 ай бұрын

    Question : the object like an agar bottle - must be kept totally in the steam or just half of them?

  • @RynaxAlien
    @RynaxAlien9 жыл бұрын

    Is vacuum also used?

  • @akhanshamenov8924
    @akhanshamenov89248 жыл бұрын

    Using autoclave is important when preparing for a surgical procedures. I wonder if it uses more energy than kettle or microwave.

  • @SpiraSpiraSpira

    @SpiraSpiraSpira

    4 жыл бұрын

    akhan shamenov Yes, an autoclave uses more energy than both. Also most autoclaves are configured to maintain a certain temperature even when not in use so as to make the sterilization process quicker. Even this idle consumption is about the same as a home microwave (1kw or so)

  • @binnykakkassery1869
    @binnykakkassery18696 жыл бұрын

    Is this use for retort pack

  • @isasamuri1061
    @isasamuri10613 жыл бұрын

    what if i use slow exhaust to streil empty glass

  • @bdlibya17
    @bdlibya1710 жыл бұрын

    thanks

  • @zahraothman1529
    @zahraothman1529 Жыл бұрын

    شكرا حبيبتي💜❤️

  • @fvasonline6205
    @fvasonline62054 жыл бұрын

    A good video.

  • @Industrialfoodssolutions
    @Industrialfoodssolutions2 жыл бұрын

    Why not use a universal sterilizer?

  • @angela-xk2ov
    @angela-xk2ov8 жыл бұрын

    when should I turn off the autoclave? after or before opening the lid once the cycle is done? help please:)

  • @nikolebryant7447
    @nikolebryant74473 жыл бұрын

    Is there a specific job in a hospital for sterilization using autoclave? I'm currently a nursing student exploring what I may want to specialize in.

  • @sparkysmom7149

    @sparkysmom7149

    2 жыл бұрын

    You would want to work in a Clinical Laboratory then

  • @billk6494
    @billk649410 жыл бұрын

    Important Videos For New Tattoo Artist! Take Care, Bill Kearney :-)

  • @ilyasjamei1413

    @ilyasjamei1413

    3 жыл бұрын

    are you a tattoo artist?

  • @diao77723
    @diao777236 жыл бұрын

    how does the pressure clean the inside of the containers? surely some extremophiles type bacteria could live on the inside of the sealed containers

  • @purugigi

    @purugigi

    6 жыл бұрын

    Considering where those extremophiles live, I highly doubt they'll make their way into an autoclave.

  • @diao77723

    @diao77723

    6 жыл бұрын

    i mean on the inside of the bottles being cleaned, their sort-of sealed so i dont know how the pressure can get inside the bottles to be cleaned

  • @kiaghods5730

    @kiaghods5730

    4 жыл бұрын

    Diarmuid Mac Mahon you’re not supposed to completely tighten the lid on bottles when putting them in the autoclave.

  • @chickensataybroccoli
    @chickensataybroccoli9 жыл бұрын

    is it 121F ? struggling here in Aus

  • @HammadAsim

    @HammadAsim

    9 жыл бұрын

    Elouise Gaylard its celsius

  • @uknowngirl2531
    @uknowngirl25317 жыл бұрын

    the embed code doesnt works in powerpoint .pls help

  • @arlix6845
    @arlix6845 Жыл бұрын

    isnt inhaling the air coming from the autoclave is bad for us

  • @PandaReefNguyen
    @PandaReefNguyen4 жыл бұрын

    FUNJEE

  • @Ajayjohnson264
    @Ajayjohnson2646 жыл бұрын

    Fun Gee

  • @pronglebeing8389

    @pronglebeing8389

    2 жыл бұрын

    hahahahahahahahahahahahahahahahahahahahahahahahah

  • @Donatellangelo
    @Donatellangelo6 жыл бұрын

    These still can't destroy prions! D:

  • @lijaucolouiserichard2320
    @lijaucolouiserichard23202 жыл бұрын

    121 degrees 15psi 15 minutes 121 degrees 15psi 20 minutes

  • @leewilliam3417
    @leewilliam34179 ай бұрын

    Mmmmnn 😊

  • @karencanaviri1173
    @karencanaviri11732 жыл бұрын

    Escape lento - Líquidos y desechos médicos Escape rápido - Cristalería e instrumentos vacíos

  • @lethalx88
    @lethalx882 жыл бұрын

    Ugh...never sterilize tattoo needles buddy.

  • @mominjaved7616
    @mominjaved7616 Жыл бұрын

    Good.. But don't use music